
Maki Ohno
Faculty of Pharmaceutical Sciences,Department of Pharmaceutical Sciences | Associate Professor |
Last Updated :2025/10/07
■Researcher basic information
■Career
Career
■Research activity information
Paper
- Anatase and rutile titanium oxide nanoparticles induce acute kidney injury by coadministration with paraquat, cisplatin or 5-aminosalicylic acid.
H Kamiyama; C Nonaka; H Saitoh; M Ohno; Y Shimizu; K Isoda
Die Pharmazie, 29 Feb. 2024, [Reviewed]
Nanoparticles are used in a variety of fields; for example, titanium oxide nanoparticles are used in paints, food additives, cosmetics, and sunscreen materials. Although the use of titanium oxide nanoparticles is regulated, their safety has not been established. Furthermore, the interaction between titanium oxide nanoparticles and various chemical substances and pharmaceuticals is unknown. We co-administered rutile-type titanium oxide nanoparticles (nTR) or anatase-type titanium oxide nanoparticles (nTA) to mice together with paraquat (PQ), cisplatin (CDDP), or anti-5-aminosalicylic acid (5-ASA), and investigated the extent, if any, of liver and kidney injury. As a result, when nTA and nTR were administered alone, no increases were observed in aspartate aminotransferase (AST) and alanine aminotransferase (ALT), which are indicators of liver damage, or urea nitrogen (BUN), which is an indicator of kidney damage. Next, nTA and nTR were co-administered with PQ, CDDP or 5-ASA. Although no increase in ALT or AST was observed, BUN levels increased significantly and acute kidney injury was induced. The findings suggested that titanium oxide nanoparticles induce acute kidney injury through their interaction with chemicals and drugs. - Addition of L-cysteine to the N- or C-terminus of the all-Denantiomer [D(KLAKLAK)2] increases antimicrobial activities against multidrug-resistant Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli
Maki K Ohno, Teruo Kirikae, Eisaku Yoshihara, Fumiko Kirikae, Isao Ishida
PeerJ, Nov. 2020, [Reviewed]
Background: Antimicrobial peptides have a broad spectrum of antimicrobial activities and are attracting attention as promising next-generation antibiotics against multidrug-resistant (MDR) bacteria. The all-d-enantiomer [D(KLAKLAK)2] has been reported to have antimicrobial activity against Escherichia coli and Pseudomonas aeruginosa, and to be resistant to protein degradation in bacteria because it is composed of D-enantiomer compounds. In this study, we demonstrated that modification of [D(KLAKLAK)2] by the addition of an L-cysteine residue to its N- or C- terminus markedly enhanced its antimicrobial activities against Gram-negative bacteria such as MDR Acinetobacter baumannii, E. coli, and P. aeruginosa. Methods: The peptides [D(KLAKLAK)2] (DP), DP to which L-cysteine was added at the N-terminus C-DP, and DP to which L-cysteine was added at the C-terminus DP-C, were synthesized at >95% purity. The minimum inhibitory concentrations of peptides and antibiotics were determined by the broth microdilution method. The synergistic effects of the peptides and the antibiotics against MDR P. aeruginosa were evaluated using the checkerboard dilution method. In order to assess how these peptides affect the survival of human cells, cell viability was determined using a Cell Counting Kit-8. Results: C-DP and DP-C enhanced the antimicrobial activities of the peptide against MDR Gram-negative bacteria, including A. baumannii, E. coli, and P. aeruginosa. The antimicrobial activity of DP-C was greater than that of C-DP, with these peptides also having antimicrobial activity against drug-susceptible P. aeruginosa and drug-resistant P. aeruginosa overexpressing the efflux pump components. C-DP and DP-C also showed antimicrobial activity against colistin-resistant E. coli harboring mcr-1, which encodes a lipid A modifying enzyme. DP-C showed synergistic antimicrobial activity against MDR P. aeruginosa when combined with colistin. The LD50 of DP-C against a human cell line HepG2 was six times higher than the MIC of DP-C against MDR P. aeruginosa. The LD50 of DP-C was not altered by incubation with low-dose colistin. Conclusion: Attachment of an L-cysteine residue to the N- or C-terminus of [D(KLAKLAK)2] enhanced its antimicrobial activity against A. baumannii, E. coli, and P. aeruginosa. The combination of C-DP or DP-C and colistin had synergistic effects against MDR P. aeruginosa. In addition, DP-C and C-DP showed much stronger antimicrobial activity against MDR A. baumannii and E. coli than against P. aeruginosa. - Sensitivity of human dental pulp cells to eighteen chemical agents used for endodontic treatments in dentistry
Morio Kobayashi; Takeo W. Tsutsui; Tomoko Kobayashi; Maki Ohno; Yukari Higo; Tomohiro Inaba; Takeki Tsutsui
ODONTOLOGY, Jan. 2013, [Reviewed] - Cell Shape Regulation Based on Hepatocyte Sheet Engineering Technologies
Soichi Takagi; Maki Ohno; Kazuo Ohashi; Rie Utoh; Kohei Tatsumi; Teruo Okano
CELL TRANSPLANTATION, 2012, [Reviewed] - Possible involvement of loss of imprinting in immortalization of human fibroblasts
Kotaro Okamura; Maki Ohno; Takeki Tsutsui
INTERNATIONAL JOURNAL OF ONCOLOGY, Apr. 2011, [Reviewed] - Development of Novel Culture System Using Nano-biotechnology
Akiyoshi Taniguchi; Ken-ichi Wada; Maki Ohno
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, Apr. 2010, [Reviewed] - Maturation of the Extracellular Matrix and Cell Adhesion Molecules in Layered Co-cultures of HepG2 and Endothelial Cells
Maki Ohno; Kiyoto Motojima; Teruo Okano; Akiyoshi Taniguchi
JOURNAL OF BIOCHEMISTRY, May 2009, [Reviewed] - Induction of Drug-Metabolizing Enzymes by Phenobarbital in Layered Co-culture of a Human Liver Cell Line and Endothelial Cells
Maki Ohno; Kiyoto Motojima; Teruo Okano; Akiyoshi Taniguchi
BIOLOGICAL & PHARMACEUTICAL BULLETIN, May 2009, [Reviewed] - Up-Regulation of Drug-Metabolizing Enzyme Genes in Layered Co-Culture of a Human Liver Cell Line and Endothelial Cells
Maki Ohno; Kiyoto Motojima; Teruo Okano; Akiyoshi Taniguchi
TISSUE ENGINEERING PART A, Nov. 2008, [Reviewed] - Up-regulation of liver functions in layered co-culture of hepatocyte and endothelial cells
Akiyoshi Taniguchi; Maki Ohno; Teruo Okano
8th World Biomaterials Congress 2008, 2008 - Expression of P450 genes in double layered co-culture using thermo-responsive culture dish
Maki Ohno; Kiyoto Motojima; Teruo Okano; Akiyoshi Taniguchi
8th World Biomaterials Congress 2008, 2008 - Clastogenic activity of seven endodontic medications used in dental practice in human dental pulp cells
Hitomi Someya; Yukari Higo; Maki Ohno; Takeo W. Tsutsui; Takeki Tsutsui
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, Jan. 2008, [Reviewed] - Ability of root canal antiseptics used in dental practice to induce chromosome aberrations in human dental pulp cells
Hiroyuki Nishimura; Yukari Higo; Maki Ohno; Takeo W. Tsutsui; Takeki Tsutsui
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, Jan. 2008, [Reviewed] - Ability of root canal antiseptics used in dental practice to induce chromosome aberrations in human dental pulp cells.
Nishimura H; Higo Y; Ohno M; Tsutsui TW; Tsutsui T
Mutation research, Jan. 2008, [Reviewed]
Root canal antiseptics are topically applied to root canals within the pulpless teeth to treat the root canal and periapical infections. Because the antiseptics that are applied to root canals can penetrate through dentin or leak out through an apical foramen into the periodontium and distribute by the systemic circulation, it is important to study the safety of these antiseptics. In the present study, we examined the ability to induce chromosome aberrations in human dental pulp cells of five root canal antiseptics, namely, carbol camphor (CC), camphorated p-monochlorophenol (CMCP), formocresol (FC), calcium hydroxide, and iodoform which are most commonly used in dental practice. Statistically significant increases in the levels of chromosome aberrations were induced by CC, FC, or iodoform in a concentration-dependent manner. Conversely, CMCP and calcium hydroxide failed to induce chromosome aberrations in the absence or presence of exogenous metabolic activation. The percentages of cells with polyploid or endoreduplication were enhanced by FC or iodoform. Our results indicate that the root canal antiseptics that exhibited a positive response are potentially genotoxic to human cells. - Clastogenic activity of seven endodontic medications used in dental practice in human dental pulp cells.
Someya H; Higo Y; Ohno M; Tsutsui TW; Tsutsui T
Mutation research, Jan. 2008, [Reviewed]
Numerous and varied chemical agents are used for endodontic treatments in dental practice. Endodontic medications are administered directly to the teeth in relatively high concentrations and chemical agents applied to enamel or dentin can penetrate the dental pulp tissue and circulate through the body in the bloodstream. In the present study, to assess safety regarding mutagenicity, we investigated the ability of seven endodontic medications to induce chromosome aberrations in human dental pulp cells. Chromosome aberrations were induced in cells treated with each of six endodontic medications, eugenol, guaiacol, modified phenol, phenol, thymol, and zinc oxide. The other endodontic medication, zinc chloride, failed to induce chromosome aberrations in the presence or absence of exogenous metabolic activation. The percentages of cells with polyploid or endoreduplication were not enhanced by any of the endodontic medications tested. Our results indicate that the endodontic medications that exhibited a positive response are potentially mutagenic to human cells. - Assessment using human dental pulp cells of clastogenicity of antiseptics used in dental practice and agents for root canal enlargement and cleaning
Itsuro Hori; Yukari Higo; Maki Ohno; Takeo W. Tsutsui; Takeki Tsutsui
ODONTOLOGY, Jul. 2007, [Reviewed] - Effects of a catechol-O-methyltransferase inhibitor on catechol estrogen-induced cellular transformation, chromosome aberrations and apoptosis in Syrian hamster embryo cells
Yutaka Hirose; Takeo W. Tsutsui; Maki Ohno; J. Carl Barrett; Takeki Tsutsui
INTERNATIONAL JOURNAL OF CANCER, Apr. 2007, [Reviewed]
MISC
Research Themes
- 多剤耐性緑膿菌に対する抗菌薬の感受性を高める一本鎖抗体の開発
01 Apr. 2021 - 31 Mar. 2024 - Three-dimensional culture of the colony-forming parenchymal hepatocytes and endothelial cells for prediction of drug metabolism
Grant-in-Aid for Young Scientists (B)
Teikyo Heisei University
2010 - 2012
Primary human hepatocytes are used extensively to study drug-metabolizing enzymes such as the cytochrome P450 enzymes. However, the activities of these enzymes decrease rapidly during culture. Thus, a more functional culture system is required to obtain the usually high levels of activity and regulation ability of drug metabolizing systems. In the present study, using a thermo-responsive culture dish, layered co-culture was achieved by placing a pulmonary artery endothelial cell sheet onto the human small hepatocyte. The expression levels of drug-metabolizing enzymes were significantly increased, compared with the monolayer cultured small hepatocytes.