Chisato Kurisaka
| Faculty of Pharmaceutical Sciences,Department of Pharmaceutical Sciences | Lecturer |
Last Updated :2026/03/31
■Researcher basic information
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■Research activity information
Paper
- IgE-Crosslinking-Induced Luciferase Expression Test as a Sensitive Indicator of Anisakis Allergy.
Haruyo Akiyama; Masashi Niwa; Chisato Kurisaka; Yuto Hamada; Yuma Fukutomi; Reiko Teshima
Antibodies (Basel, Switzerland), 25 Feb. 2025, [Reviewed]
Background:Anisakis allergy has been increasing, and the diagnosis of it is based on specific serum IgE detection. Recently, the IgE-crosslinking-induced luciferase expression (EXiLE) test has been proposed as convenient tool for detecting functionally specific IgE antibodies. Here, we investigated if the EXiLE test is a useful tool in the diagnosis of Anisakis allergy. Methods: HuRa-40 cells were sensitized using six serum types from three patients with Anisakis allergy at the time of the initial test and after 6-12 months. Thereafter, various concentrations of Anisakis worm protein (AWP) were reacted to measure the degree of EXiLE. The degree of EXiLE was compared with Anisakis-specific IgE antibody levels measured by the CAP-FEIA method, and the IgE-antibody-binding protein profile was examined using IgE immunoblotting. Results: The results showed a good correlation between the CAP-FEIA values and EXiLE obtained with 5 μg/mL of AWP (R = 0.91, p < 0.01), a strong response on IgE immunoblotting in the region containing proteins weighing ≥40,000 Da. In addition, after the onset of Anisakis allergy, the degree of serum EXiLE decreased in two patients whose Anisakis-specific IgE antibody levels decreased over time but increased in one patient whose specific IgE antibodies increased after repeated antigen sensitization. Conclusions: Based on these data, the AWP-induced EXiLE test seemed to be useful and convenient for the diagnosis of Anisakis allergy, supplementing specific IgE determinants. After allergy onset, the use of this method to observe changes in specific IgE levels over time may be important for predicting the risk of recurrence. - Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells.
Haruyo Akiyama; Chisato Kurisaka; Kenichi Kumasaka; Ryosuke Nakamura
Journal of immunological methods, Jun. 2024, [Reviewed]
BACKGROUND: The measurement of antigen-specific serum IgE is common in clinical assessments of type I allergies. However, the interaction between antigens and IgE won't invariably trigger mast cell activation. We previously developed the IgE crosslinking-induced luciferase expression (EXiLE) method using the RS-ATL8 mast cell line; however, the method may not be sensitive enough in some cases. METHODS: In this study, we introduced an NF-AT-regulated luciferase reporter gene into the RBL-2H3 rat mast cell line and expressed a chimeric high-affinity IgE receptor (FcεRI) α chain gene, comprising an extracellular domain from humans and transmembrane/intracellular domains from rats. RESULTS: We generated multiple clones expressing the chimeric receptor. Based on their responsiveness and proliferation, we selected the HuRa-40 clone. This cell line exhibited significantly elevated human α chain expression compared to RS-ATL8 cells, demonstrating a 10-fold enhancement of antigen-specific reactivity. Reproducibility across different batches and operators was excellent. Moreover, we observed a detectable response inhibition by an anti-allergy drugs (omalizumab and cyclosporin A). CONCLUSIONS: HuRa-40 cells-which carry the human-rat chimeric IgE receptor-comprise a valuable reporter cell line for the EXiLE method. Their versatility extends to various applications and facilitates high-throughput screening of anti-allergy drugs. - Consideration of the EXiLE test for predicting anaphylaxis after diclofenac etalhyaluronate administration
Haruyo Akiyama; Chisato Kurisaka; Dai Muramatsu; Shuhei Takada; Kei Toyama; Keiji Yoshioka; Ryosuke Nakamura
Journal of Immunotoxicology, 2024, [Reviewed] - Identification of human plasma C1 inhibitor as a target protein for staphylococcal superantigen-like protein 5 (SSL5).
Oku T; Kurisaka C; Ando Y; Tsuji T
Biochemical and biophysical research communications, Jan. 2019, [Reviewed] - Stimulation of Peritoneal Mesothelial Cells to Secrete Matrix Metalloproteinase-9 (MMP-9) by TNF-α: A Role in the Invasion of Gastric Carcinoma Cells.
Oku T; Shimada K; Kenmotsu H; Ando Y; Kurisaka C; Sano R; Tsuiji M; Hasegawa S; Fukui T; Tsuji T
International journal of molecular sciences, Dec. 2018, [Reviewed] - Generation of a Monoclonal Antibody Against Staphylococcal Superantigen-Like Protein 5 (SSL5) That Discriminates SSL5 from Other SSL Proteins.
Oku T; Soma H; Kurisaka C; Tsuji T
Monoclonal antibodies in immunodiagnosis and immunotherapy, Nov. 2018, [Reviewed] - Role of sialic acid-containing glycans of matrix metalloproteinase-9 (MMP-9) in the interaction between MMP-9 and staphylococcal superantigen-like protein 5
Chisato Kurisaka; Teruaki Oku; Saotomo Itoh; Tsutomu Tsuji
Microbiology and Immunology, 01 Mar. 2018, [Reviewed]
MISC
- IgE依存型アレルギーにおける第2世代EXiLE法と既存検査法との比較検討
Oct. 2025 - EXiLE法によるJOYCLU®誘発アナフィラキシーの要因検出における有用性評価
Oct. 2025 - アニサキスアレルギー診断におけるEXiLE法の有用性評価
Oct. 2025 - 病態・薬物治療学分野における薬学モデル・コア・カリキュラム（令和4年度改訂版）に基づく薬剤師国家試験対策
Aug. 2025 - Analysis of Generative AI Responses to Pharmacist National Examination Questions and Educational Implications
Aug. 2025 - Search for mast cell activation inhibitors using chimeric IgE receptor-expressing cell line HuRa-40
栗坂知里; 大山朋希; 村尾千帆; 相澤優帆; 岩田悠郁; 中村亮介; 秋山晴代
日本薬学会年会要旨集(Web), 2025 - Correlation of treatment response to Japanese cedar pollen SLIT and EC50 of EXiLE response
栗坂知里; 菅原健; 蛯名梨音; 齊藤美優; 中村亮介; 土井雅津代; 秋山晴代
日本薬学会年会要旨集(Web), 2024 - Development of in vitro allergy testing method using saliva
秋山晴代; 栗坂知里; 小川裕子; 大橋知子; 皆川範亨; 原田佳英; 渡部明日香; 池本守; 中村亮介; 矢ノ下良平
日本薬学会年会要旨集(Web), 2024 - Development of high-throughput screening method for environmental allergy exacerbating substances by evaluation of human IgE cross-linking activity
秋山晴代; 栗坂知里; 渡部明日香; 大橋知子; 原田佳英; 中村亮介
日本免疫毒性学会学術年会講演要旨集, 2023 - Establishment of high-throughput screening system to evaluate IgE cross-linking activity.
秋山晴代; 栗坂知里; 石井巧; 佐々木彩夢; 原有彩; 中村亮介
日本薬学会年会要旨集(Web), 2023 - 高感度I型アレルギー試験法の開発を目的としたIgEキメラ受容体発現細胞の作製
Mar. 2022 - Development of highly-sensitive allergy test using chimeric IgE receptor-expressing cultured mast cells
秋山晴代; 栗坂知里; 田所哲; 熊坂謙一; 中村亮介
日本免疫毒性学会学術年会講演要旨集, 2022 - コロナ禍におけるセミナー科目のオンライン化対応と授業効果測定の検証
Mar. 2021 - 加熱・加圧処理による魚肉低アレルゲン化の検討 EXiLE法による応答性評価
Oct. 2020 - EXiLE法を用いた加熱・加圧処理による魚肉アレルゲン性低減化の評価
Mar. 2020 - 黄色ブドウ球菌SSL5およびSSL11の白血球MMP-9に与える影響
Sep. 2019 - 黄色ブドウ球菌Staphylococcal superantigen‐like protein 5(SSL5)の宿主免疫系に与える影響
奥輝明; 栗坂知里; 栗坂知里; 安藤祐介; 安藤祐介; 人見祐基; 築地信; 伊藤佐生智; 伊藤佐生智; 辻勉, 辻勉
Pharmaco-Hematologyシンポジウム講演要旨集, 08 Jun. 2019 - 黄色ブドウ球菌Staphylococcal superantigen‐like protein5(SSL5)はヒト血漿C1インヒビターに結合する
奥輝明; 栗坂知里; 安藤祐介; 辻勉
日本薬学会年会要旨集(CD-ROM), 2019 - Staphylococcal superantigen‐like protein5とMatrix metalloproteinase9の結合
栗坂知里; 奥輝明; 伊藤佐生智; 伊藤佐生智; 辻勉
Pharmaco-Hematologyシンポジウム講演要旨集, 13 Jun. 2015
Research Themes
- IgE架橋を近接ライゲーションアッセイで捉える高感度セルフリーアレルギー試験の開発
01 Apr. 2023 - 31 Mar. 2027 - 唾液検体を用いたin vitroアレルギー試験法の確立
01 Apr. 2023 - 31 Mar. 2027 - スギ花粉舌下免疫療法の奏効メカニズム解明と奏効性予測法の確立
01 Apr. 2020 - 31 Mar. 2023 - IgEの架橋活性を指標とする新規セルフリーアレルギー試験法の開発
01 Apr. 2020 - 31 Mar. 2023
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