Feb. 2024
The Scientific Research Honor Society, USA, Full Member, Sigma Xi
Scholarly achievements and contributions to the advancement of knowledge
Takashi Izumi

Mar. 1978

AVID mouse: A versatile platform for real-time, multiscale ATP imaging and spatial systems metabolism analysis in living mice
Yuichiro Ohnishi; Daiki Setoyama; Hideki Miwa; Norimichi Koitabashi; Riki Ogasawara; Seri Kitada; Naoki Matoba; Takahito Ayano; Kaoru Hiramoto; Ryuto Yasui; Yuki Sugiura; Takahisa Anada; Kosuke Ino; Hinako Matsuda; Takahiro Noma; Shigenori Nonaka; Takashi Izumi; Masahiko Kurabayashi; Makoto Suematsu; Yuya Kunisaki; Motoko Yanagita; Hiromi Imamura; Masamichi Yamamoto
Cell Reports, 12 Sep. 2025, [Reviewed]

Ultrasensitive Specific Detection of Anti-influenza A H1N1 Hemagglutinin Monoclonal Antibody Using Silicon Nanowire Field Effect Biosensors
Hui Zhang; Fumiya Osawa; Haru Okamoto; Yawei Qiu; Zhiheng Liu; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
ACS Applied Bio Materials, 15 Jan. 2025, [Reviewed]

Branched-chain amino acid metabolism is a crucial modulator of cellular senescence
Yuma Aramaki; Kazuki Irie; Hideru Obinata; Shinya Honda; Takuro Horii; Satoko Arakawa; Aiko Tsuchida; Junki Hoshino; Ryosuke Kobayashi; Takashi Izumi; Izuho Hatada; Shigeomi Shimizu; Yoji A. Minamishima; Akimitsu Konishi
10 Sep. 2024
Abstract

Cellular senescence is a complex stress response that results in the permanent arrest of cell proliferation. The accumulation of senescent cells occurs during aging in living organisms, and contributes to tissue dysfunction. Although there are growing lines of evidence that various metabolic changes occur in senescent cells, the link between cellular metabolism and senescence is not yet fully understood. In this study, we demonstrate that alterations in the metabolism of branched-chain amino acids (BCAAs) play a crucial role in establishing cellular senescence. Furthermore, we identified mitochondrial BCAA transamination as a crucial step in this process. Our findings show that various types of cellular stress lead to a reduction in the expression of BCAA aminotransferase 2 (BCAT2), one of the BCAA catabolic enzymes, resulting in decreased catabolism of BCAAs and reduced synthesis of glutamate. The reduction of BCAA catabolites, together with the consequent limitation in glutathione production from glutamate, triggers cellular senescence. Furthermore, we demonstrate that a reduction in BCAT2 levels alone is sufficient to induce cellular senescence, both in cultured cells and in mice. Additionally, our results demonstrate that aging alters BCAA metabolism in both mice and humans. Our findings provide new insights into the metabolic mechanisms underlying cellular senescence, with a particular focus on the role of BCAAs.

GDE5/Gpcpd1 activity determines phosphatidylcholine composition in skeletal muscle and regulates contractile force in mice
Rahmawati Aisyah; Noriyasu Ohshima; Daiki Watanabe; Yoshiko Nakagawa; Tetsushi Sakuma; Felix Nitschke; Minako Nakamura; Koji Sato; Kaori Nakahata; Chihiro Yokoyama; Charlotte R. Marchioni; Thanutchaporn Kumrungsee; Takahiko Shimizu; Yusuke Sotomaru; Toru Takeo; Naomi Nakagata; Takashi Izumi; Shinji Miura; Berge A. Minassian; Takashi Yamamoto; Masanobu Wada; Noriyuki Yanaka
Communications Biology, 20 May 2024, [Reviewed]
Abstract

Glycerophosphocholine (GPC) is an important precursor for intracellular choline supply in phosphatidylcholine (PC) metabolism. GDE5/Gpcpd1 hydrolyzes GPC into choline and glycerol 3-phosphate; this study aimed to elucidate its physiological function in vivo. Heterozygous whole-body GDE5-deficient mice reveal a significant GPC accumulation across tissues, while homozygous whole-body knockout results in embryonic lethality. Skeletal muscle-specific GDE5 deletion (Gde5 skKO) exhibits reduced passive force and improved fatigue resistance in electrically stimulated gastrocnemius muscles in vivo. GDE5 deficiency also results in higher glycolytic metabolites and glycogen levels, and glycerophospholipids alteration, including reduced levels of phospholipids that bind polyunsaturated fatty acids (PUFAs), such as DHA. Interestingly, this PC fatty acid compositional change is similar to that observed in skeletal muscles of denervated and Duchenne muscular dystrophy mouse models. These are accompanied by decrease of GDE5 expression, suggesting a regulatory role of GDE5 activity for glycerophospholipid profiles. Furthermore, a DHA-rich diet enhances contractile force and lowers fatigue resistance, suggesting a functional relationship between PC fatty acid composition and muscle function. Finally, skinned fiber experiments show that GDE5 loss increases the probability of the ryanodine receptor opening and lowers the maximum Ca²⁺-activated force. Collectively, GDE5 activity plays roles in PC and glucose/glycogen metabolism in skeletal muscle.

Capillary electrophoresis using triple layer modified capillary facilitating salivary ion analyses: Application to search for potential stress markers induced by cold pressure test
Hironori Myochin; Noriyasu Ohshima; Takashi Izumi; Tatsuya Hisajima; Romanas Chaleckis; Masanobu Mori
Journal of Chromatography A, 12 Apr. 2024, [Reviewed]

The possibility of ultrasensitive detection of biomolecules using silicon nanowire biosensor with structural optimization
Hui Zhang; Mayuna Abe; Fumiya Osawa; Yawei Qiu; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
Japanese Journal of Applied Physics, 12 Mar. 2024, [Reviewed]
Abstract

Silicon nanowire (SiNW) biosensors, operating as field-effect transistors (FETs), demonstrate remarkable capabilities for the ultrasensitive detection of specific biomolecules. Our prior work specifically explored the impact of SiNW widths on biosensor sensitivity, highlighting that narrower SiNWs significantly enhance detection sensitivity. While experimental studies provide valuable insights, theoretical investigations into the combined effect of multiple parameters on sensing performance are crucial. However, theoretical studies have been relatively scarce in the research of SiNW biosensors. In response to this gap, we developed a numerical model of SiNW biosensor using the finite-element method in COMSOL Multiphysics. By leveraging simulations, we explored the sensing performance of SiNW biosensors across various widths, thicknesses, impurity concentrations, and their combined effects, addressing a previously unexplored area in this research. Based on the simulations, the optimal structure that exhibits both high sensitivity and measurable current was predicted. To ascertain the reliability of our simulations, a subset of the results was compared with experimental data. Our findings indicate the potential for achieving ultrasensitive biomolecule detection using SiNW biosensors through structural optimization.

Clinical effect of phytosterol-enriched saw palmetto extract on urinary function in healthy middle-aged and older males: a double-blind, placebo-controlled randomized comparative study
Hideaki Waki; Takuya Suzuki; Takashi Izumi; Tatsuya Hisajima
Functional Foods in Health and Disease, 09 Feb. 2024, [Reviewed]
Background: Saw palmetto extract (SPE) is one of the most widely used supplements for benign prostatic hyperplasia (BPH) due to its pharmacological effects. β-sitosterol-enriched saw palmetto oil (VISPO™) contains more than 3% β-sitosterol and 85% total fatty acids and has been shown to improve symptoms in patients with BPH. Lower urinary tract symptoms (LUTS) require primary preventive measures in countries with accelerated population aging, including Japan. VISPO™ is expected to be effective against the early symptoms of LUTS. However, the effect of VISPO™ on urinary function in healthy individuals has not been elucidated.Objective: This clinical study evaluated the efficacy and tolerability of VISPO™ in the management of urinary complaints among healthy middle-aged and older men who did not require treatment.Methods: We conducted a randomized, double-blind, placebo-controlled trial that included 54 male participants who were assigned to consume VISPO™ or placebo (olive oil) capsules for 12 weeks. Biometric data, uroflowmetry results, and salivary hormone levels were assessed at weeks 1 and 12. Urinary function and quality of life were assessed at weeks 1, 4, 8, and 12 using the International Prostate Symptom Score (IPSS), Overactive Bladder Symptom Score (OABSS), Core Lower Urinary Tract Symptom Score (CLSS), and WHOQOL-26.Results: Two-way analysis of variance revealed that IPSS and OABSS were significantly lower in the VISPO™ group that in the placebo group. However, there were no significant differences in urine volume, urinary flow rate, or salivary hormone levels. In addition, no participants reported adverse effects (diarrhea, abdominal pain, etc.). Conclusion: The results of the present study, which is the first randomized controlled trial of VISPO™ conducted among healthy participants, demonstrate the effectiveness of VISPO™ in the management of lower urinary tract complaints that do not require treatment. Trial Registration: University Hospital Medical Information Network Clinical Trials Registry (UMIN000046386).Keywords: saw palmetto, VISPO™, β-sitosterol, fatty acids, healthy subjects, primary preventive, IPSS, OABSS, lower urinary tract symptoms (LUTS)

Estimation of the Depletion Layer Thickness in Silicon Nanowire-Based Biosensors from Attomolar-Level Biomolecular Detection
Hui Zhang; Yawei Qiu; Fumiya Osawa; Meiko Itabashi; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
ACS Applied Materials & Interfaces, 12 Apr. 2023, [Reviewed]
Silicon nanowire (SiNW) biosensors have attracted a lot of attention due to their superior sensitivity. Recently, the dependence of biomolecule detection sensitivity on the nanowire (NW) width, number, and doping density has been partially investigated. However, the primary reason for achieving ultrahigh sensitivity has not been elucidated thus far. In this study, we designed and fabricated SiNW biosensors with different widths (10.8-155 nm) by integrating a complementary metal-oxide-semiconductor process and electron beam lithography. We aimed to investigate the detection limit of SiNW biosensors and reveal the critical effect of the 10-nm-scaled SiNW width on the detection sensitivity. The sensing performance was evaluated by detecting antiovalbumin immunoglobulin G (IgG) with various concentrations (from 6 aM to 600 nM). The initial thickness of the depletion region of the SiNW and the changes in the depletion region due to biomolecule binding were calculated. The basis of this calculation are the resistance change ratios as functions of IgG concentrations using SiNWs with different widths. The calculation results reveal that the proportion of the depletion region over the entire SiNW channel is the essential reason for high-sensitivity detection. Therefore, this study is crucial for an indepth understanding on how to maximize the sensitivity of SiNW biosensors.

PPARα activation partially drives NAFLD development in liver-specific Hnf4a-null mice
Carlos Ichiro Kasano-Camones; Masayuki Takizawa; Noriyasu Ohshima; Chinatsu Saito; Wakana Iwasaki; Yuko Nakagawa; Yoshio Fujitani; Ryo Yoshida; Yoshifumi Saito; Takashi Izumi; Shin-Ichi Terawaki; Masakiyo Sakaguchi; Frank J Gonzalez; Yusuke Inoue
The Journal of Biochemistry, 23 Jan. 2023, [Reviewed]
Abstract

HNF4α regulates various genes to maintain liver function. There have been reports linking HNF4α expression to the development of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis. In this study, liver-specific Hnf4a-deficient mice (Hnf4aΔHep mice) developed hepatosteatosis and liver fibrosis, and they were found to have difficulty utilizing glucose. In Hnf4aΔHep mice, the expression of fatty acid oxidation-related genes, which are PPARα target genes, was increased in contrast to the decreased expression of PPARα, suggesting that Hnf4aΔHep mice take up more lipids in the liver instead of glucose. Furthermore, Hnf4aΔHep/Ppara−/− mice, which are simultaneously deficient in HNF4α and PPARα, showed improved hepatosteatosis and fibrosis. Increased C18:1 and C18:1/C18:0 ratio was observed in the livers of Hnf4aΔHep mice, and the transactivation of PPARα target gene was induced by C18:1. When the C18:1/C18:0 ratio was close to that of Hnf4aΔHep mouse liver, a significant increase in transactivation was observed. In addition, the expression of Pgc1a, a coactivator of PPARs, was increased, suggesting that elevated C18:1 and Pgc1a expression could contribute to PPARα activation in Hnf4aΔHep mice. These insights may contribute to the development of new diagnostic and therapeutic approaches for NAFLD by focusing on the HNF4α and PPARα signaling cascade.

Characterization of recombinant murine GDE4 and GDE7, enzymes producing lysophosphatidic acid and/or cyclic phosphatidic acid.
Binderiya Tserendavga; Noriyasu Ohshima; Chiaki Fujita; Koji Yuzawa; Mari Ohshima; Noriyuki Yanaka; Yoji Andrew Minamishima; Takashi Izumi
Journal of biochemistry, 15 Sep. 2021, [Reviewed]
GDE4 and GDE7 are membrane-bound enzymes that exhibit lysophospholipase D (lysoPLD) activities. We found that GDE7 produced not only lysophosphatidic acid (LPA) but also cyclic phosphatidic acid (cPA) from lysophospholipids by a transphosphatidylation reaction. In contrast, GDE4 produced only LPA. The analysis of substrate specificity showed that 1-alkyl-lysophosphospholipids were preferred substrates for both enzymes rather than 1-alkyl-lysophospholipids and 1-alkenyl-lysophospholipids. Among the various lysophospholipids with different polar head groups that were tested, lysophosphatidylglycerol and lysophosphatidylserine were preferred substrates for GDE4 and GDE7, respectively. The detailed analysis of the dependency of the enzyme activities of GDE4 and GDE7 on divalent cations suggested multiple divalent cations were bound in the active sites of both enzymes. Taken together, these results suggest the possibility that GDE7 functions as a cPA-producing enzyme in the body.

High-Precision Automated Workflow for Urinary Untargeted Metabolomic Epidemiology
Isabel Meister; Pei Zhang; Anirban Sinha; C. Magnus Sköld; Åsa M. Wheelock; Takashi Izumi; Romanas Chaleckis; Craig E. Wheelock
Analytical Chemistry, 19 Mar. 2021, [Reviewed]

Simultaneous capillary electrophoresis of anions and cations in a single injection using an anion exchanger-modified capillary for determination of salivary ions in combination with statistical analyses
Masanobu Mori; Keisuke Aoyagi; Toshihiro Tomoda; Fumi Ishikawara; Shou Sakamoto; Hironori Myochin; Midori Kuga; Daisuke Kozaki; Noriyasu Ohshima; Takashi Izumi; Hideyuki Itabashi; Yoshifumi Shoho; Akihiro Yoshida; Katsuhiko Tsunekawa; Takao Kimura; Masami Murakami
Journal of Chromatography A, 04 Jan. 2021, [Reviewed]

Design and Fabrication of Silicon Nanowire-Based Biosensors with Integration of Critical Factors: Toward Ultrasensitive Specific Detection of Biomolecules
Hui Zhang; Naoki Kikuchi; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
ACS Applied Materials & Interfaces, 03 Nov. 2020, [Reviewed]

CD8+ regulatory T cells are critical in prevention of autoimmune-mediated diabetes.
Chikako Shimokawa; Tamotsu Kato; Tadashi Takeuchi; Noriyasu Ohshima; Takao Furuki; Yoshiaki Ohtsu; Kazutomo Suzue; Takashi Imai; Seiji Obi; Alex Olia; Takashi Izumi; Minoru Sakurai; Hirokazu Arakawa; Hiroshi Ohno; Hajime Hisaeda
Nature communications, 22 Apr. 2020, [Reviewed]
Type 1 diabetes (T1D) is an autoimmune disease in which insulin-producing pancreatic β-cells are destroyed. Intestinal helminths can cause asymptomatic chronic and immunosuppressive infections and suppress disease in rodent models of T1D. However, the underlying regulatory mechanisms for this protection are unclear. Here, we report that CD8+ regulatory T (Treg) cells prevent the onset of streptozotocin -induced diabetes by a rodent intestinal nematode. Trehalose derived from nematodes affects the intestinal microbiota and increases the abundance of Ruminococcus spp., resulting in the induction of CD8+ Treg cells. Furthermore, trehalose has therapeutic effects on both streptozotocin-induced diabetes and in the NOD mouse model of T1D. In addition, compared with healthy volunteers, patients with T1D have fewer CD8+ Treg cells, and the abundance of intestinal Ruminococcus positively correlates with the number of CD8+ Treg cells in humans.

The role of autonomously secreted PGE2 and its autocrine/paracrine effect on bone matrix mineralization at the different stages of differentiating MC3T3-E1 cells.
H Suzuki; N Ohshima; K Tatei; T Taniguchi; S Sato; T Izumi
Biochem Biophys Res Commun, Feb. 2020, [Reviewed]

Fabrication of Ultrahigh-Sensitivity Silicon Nanowire Biosensor by CMOS-Compatible Process for Detecting Biomolecules at Low Concentrations　　　　　　　　　　　　　　　
Hui Zhang; Kei Okabe; Naoki Kikuchi; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
Proceedings of International Conference on Mechanical, Electrical and Medical Intelligent System 2019 (ICMEMIS2019), Dec. 2019, [Reviewed], [Invited]

Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM- and albumin-deficient mice
Hideru Obinata; Andrew Kuo; Yukata Wada; Steven Swendeman; Catherine H. Liu; Victoria A. Blaho; Rieko Nagumo; Kenichi Sato; Takashi Izumi; Timothy Hla
The Journal of Lipid Research, Nov. 2019, [Reviewed]

Light Stress-Induced Increase of Sphingosine 1-Phosphate in Photoreceptors and Its Relevance to Retinal Degeneration.
Terao R; Honjo M; Ueta T; Obinata H; Izumi T; Kurano M; Yatomi Y; Koso H; Watanabe S; Aihara M
Int J Mol Sci., Jul. 2019, [Reviewed]
Sphingosine 1-phosphate (S1P) is a potent lipid mediator that modulates inflammation and angiogenesis. In this study, we investigated the possible involvement of S1P in the pathology of light-induced retinal degeneration in vivo and in vitro. The intracellular S1P and sphingosine kinase (SphK) activity in a photoreceptor cell line (661W cells) was significantly increased by exposure to light. The enhancement of SphK1 expression was dependent on illumination, and all-trans-retinal significantly promoted SphK1 expression. S1P treatment reduced protein kinase B (Akt) phosphorylation and increased the protein expression of cleaved caspase-3, and induced photoreceptor cell apoptosis. In vivo, light exposure enhanced the expression of SphK1 in the outer segments of photoreceptors. Intravitreal injection of a SphK inhibitor significantly suppressed the thinning of the outer nuclear layer and ameliorated the attenuation of the amplitudes of a-waves and b-waves of electroretinograms during light-induced retinal degeneration. These findings imply that light exposure induces the synthesis of S1P in photoreceptors by upregulating SphK1, which is facilitated by all-trans-retinal, causing retinal degeneration. Inhibition of this enhancement may be a therapeutic target of outer retinal degeneration, including age-related macular degeneration.

Regulation of MC3T3-E1 differentiation by actin cytoskeleton through lipid mediators reflecting the cell differentiation stage.
Hiraku Suzuki; Kazuaki Tatei; Noriyasu Ohshima; Seiichi Sato; Takashi Izumi
Biochemical and biophysical research communications, 25 Jun. 2019, [Reviewed]
Actin cytoskeleton is reported to be related in various functions of osteoblast, bone-forming cell. However the function of actin cytoskeleton in osteoblasts is not fully understood, since bone formation is derived from extracellular interactions of functional proteins produced from osteoblasts, including osteocalcin (Ocn), and it is a result of closely and complex organized sequence of biochemical events. In this study, we showed that actin cytoskeleton of MC3T3-E1 cells functioned in recognition of cell condition and regulation of extracellular matrix mineralization, bone formation. Maturation of MC3T3-E1 cells by 14 days of culture reduced F-actin filaments, while induced expression of Ocn mRNA known as late stage differentiation marker and matrix mineralization, terminal stage of cell differentiation. The disruption of actin cytoskeleton with Cyto D in immature MC3T3-E1 cells significantly increased expression of Ocn mRNA in 24 h. Both PTX-induced inhibition of signal transduction through GPCRs and celecoxib-induced suppression of lipid mediators in immature MC3T3-E1 cells reduced actin filaments and suppressed matrix mineralization. Furthermore, addition of lipid mediators extracted from culture mediums of differentiated MC3T3-E1 cells by Bligh-Dyer method induced actin cytoskeleton reorganization and matrix mineralization change in MC3T3-E1 cells. Taken together, our data suggest that actin cytoskeleton of MC3T3-E1 cells regulates activation of developmental pathway reflecting cell differentiation stages through lipid mediators. The function we identified is important for bone formation tightly regulated by mechanical stress, since actin cytoskeleton is also known as a mechanosensor of osteoblasts.

Fabrication of N-Type Silicon Nanowire Biosensor for Sub-10-Femtomolar Concentration of Immunoglobulin
Tashiro Tomoya; Hui Zhang; Kakeru Oshima; Yuya Sakurai; Takaaki Suzuki; Noriyasu Ohshima; Takashi Izumi; Hayato Sone
Key Engineering Materials, Nov. 2018, [Reviewed]
A simple fabrication process of an n-type silicon nanowire (SiNW) biosensor for sub-10 femtomolar (fM) concentration immunoglobulin detection was presented in this work. The SiNWs with different widths of 80-190 nm were fabricated using electron beam lithography and reaction ion etching techniques. The electrical characteristics of SiNWs with various widths were measured. And it can be observed that thin SiNW has high resistance, which is in agreement with electrical resistance theory. Furthermore, the surface of the fabricated SiNW was functionalized by 3-aminopropyltriethoxysilane for making the biosensor device to detect the binding of immunoglobulin G (IgG) molecules. The responsivity of the biosensor was investigated by observing electrical performance in response due to IgG with various concentration from 6 fM to 600 nanomolar (nM). The resistance changing ratio based on the current voltage (I-V) characteristics was analyzed and it increased with increasing of the IgG concentration. As a result, it demonstrated that the n-type SiNW biosensor has the ability to detect the IgG molecules with low concentration of 6 fM.

GDE5 Inhibition Accumulates Intracellular Glycerophosphocholine and Suppresses Adipogenesis at a Mitotic Clonal Expansion Stage
Yuri Okazaki; Keishi Nakamura; Shuto Takeda; Ikumi Yoshizawa; Fumiyo Yoshida; Noriyasu Ohshima; Takashi Izumi; Janet Klein; Thanutchaporn Kumrungsee; Jeff Sands; Noriyuki Yanaka
American Journal of Physiology, Nov. 2018, [Reviewed]

Inhibition of Phosphorylated c-Jun NH(2)-terminal Kinase by 2 ',4 '-dihydroxy-6-methoxy-3,5-dimethylchalcone Isolated from Eugenia aquea Burm f. Leaves in Jurkat T-cells
Melisa I. Barliana; Ajeng Diantini; Anas Subarnas; Rizky Abdulah; Takashi Izumi
PHARMACOGNOSY MAGAZINE, Jul. 2017, [Reviewed]

Fabrication of Silicon Nanowire Biosensor Using Electron Beam Lithography and Low-Concentration Antibody Detection
電気学会バイオ・マイクロシステム研究会資料, 2017, [Reviewed]

Enzymatic characterization of recombinant rat DDHD2: a soluble diacylglycerol lipase
Mari Araki; Noriyasu Ohshima; Chizu Aso; Akimitsu Konishi; Hideru Obinata; Kazuaki Tatei; Takashi Izumi
JOURNAL OF BIOCHEMISTRY, Nov. 2016, [Reviewed]

TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends
Akimitsu Konishi; Takashi Izumi; Shigeomi Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2016, [Reviewed]

Protein purification and cloning of diacylglycerol lipase from rat brain
Chizu Aso; Mari Araki; Noriyasu Ohshima; Kazuaki Tatei; Tohko Hirano; Hideru Obinata; Mikiko Kishi; Koji Kishimoto; Akimitsu Konishi; Fumio Goto; Hiroyuki Sugimoto; Takashi Izumi
JOURNAL OF BIOCHEMISTRY, Jun. 2016, [Reviewed]

New Members of the Mammalian Glycerophosphodiester Phosphodiesterase Family GDE4 AND GDE7 PRODUCE LYSOPHOSPHATIDIC ACID BY LYSOPHOSPHOLIPASE D ACTIVITY
Noriyasu Ohshima; Takahiro Kudo; Yosuke Yamashita; Stefania Mariggio; Mari Araki; Ayako Honda; Tomomi Nagano; Chiaki Isaji; Norihisa Kato; Daniela Corda; Takashi Izumi; Noriyuki Yanaka
JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 2015, [Reviewed]

Nicotine exposure alters human vascular smooth muscle cell phenotype from a contractile to a synthetic type
Shinji Yoshiyama; Zhenyi Chen; Tsuyoshi Okagaki; Kazuhiro Kohama; Ritsuko Nasu-Kawaharada; Takashi Izumi; Noriyasu Ohshima; Takeharu Nagai; Akio Nakamura
ATHEROSCLEROSIS, Dec. 2014, [Reviewed]

医師の生涯教育のニーズ ー臨床研究と研修に関してー
Oct. 2014

Expression pattern of class I phosphoinositide 3-kinase and distribution of its product, phosphatidylinositol-3,4,5-trisphosphate, during Drosophila embryogenesis
Xin Xi; Kazuaki Tatei; Yumiko Kihara; Takashi Izumi
GENE EXPRESSION PATTERNS, Jul. 2014, [Reviewed]

Neutralization of leukotriene C4 and D4 activity by monoclonal and single-chain antibodies.
Kawakami Y; Hirano S; Kinoshita M; Otsuki A; Suzuki-Yamamoto T; Suzuki M; Kimoto M; Sasabe S; Fukushima M; Kishimoto K; Izumi T; Oga T; Narumiya S; Sugahara M; Miyano M; Yamamoto S; Takahashi Y
Biochimica et biophysica acta, Jun. 2014, [Reviewed]
BACKGROUND: Cysteinyl leukotrienes (LTs) are key mediators in inflammation. To explore the structure of the antigen-recognition site of a monoclonal antibody against LTC4 (mAbLTC), we previously isolated full-length cDNAs for heavy and light chains of the antibody and prepared a single-chain antibody comprising variable regions of these two chains (scFvLTC). METHODS: We examined whether mAbLTC and scFvLTC neutralized the biological activities of LTC4 and LTD4 by competing their binding to their receptors. RESULTS: mAbLTC and scFvLTC inhibited their binding of LTC4 or LTD4 to CysLT1 receptor (CysLT1R) and CysLT2 receptor (CysLT2R) overexpressed in Chinese hamster ovary cells. The induction by LTD4 of monocyte chemoattractant protein-1 and interleukin-8 mRNAs in human monocytic leukemia THP-1 cells expressing CysLT1R was dose-dependently suppressed not only by mAbLTC but also by scFvLTC. LTC4- and LTD4-induced aggregation of mouse platelets expressing CysLT2R was dose-dependently suppressed by either mAbLTC or scFvLTC. Administration of mAbLTC reduced pulmonary eosinophil infiltration and goblet cell hyperplasia observed in a murine model of asthma. Furthermore, mAbLTC bound to CysLT2R antagonists but not to CysLT1R antagonists. CONCLUSIONS: These results indicate that mAbLTC and scFvLTC neutralize the biological activities of LTs by competing their binding to CysLT1R and CysLT2R. Furthermore, the binding of cysteinyl LT receptor antagonists to mAbLTC suggests the structural resemblance of the LT-recognition site of the antibody to that of these receptors. GENERAL SIGNIFICANCE: mAbLTC can be used in the treatment of inflammatory diseases such as asthma.

復帰支援に求められること-「医師の生涯教育・復帰支援に関するアンケート調査」より-
Aug. 2013

Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine gamma-lyase, an animal model of cystathioninuria
Hidenori Yamada; Noriyuki Akahoshi; Shotaro Kamata; Yoshifumi Hagiya; Takako Hishiki; Yoshiko Nagahata; Tomomi Matsuura; Naoharu Takano; Masatomo Mori; Yasuki Ishizaki; Takashi Izumi; Yoshito Kumagai; Tadashi Kasahara; Makoto Suematsu; Isao Ishii
FREE RADICAL BIOLOGY AND MEDICINE, May 2012, [Reviewed]

Azelnidipine is a calcium blocker that attenuates liver fibrosis and may increase antioxidant defence
T. Ohyama; K. Sato; K. Kishimoto; Y. Yamazaki; N. Horiguchi; T. Ichikawa; S. Kakizaki; H. Takagi; T. Izumi; M. Mori
BRITISH JOURNAL OF PHARMACOLOGY, Feb. 2012, [Reviewed]

【リン脂質代謝と脂質メディエーター研究の最新の成果】 (第2部)リゾリン脂質を中心とした脂質メディエーター 脂質メディエーターとしての酸化遊離脂肪酸 G2Aの役割を中心に
Jun. 2011, [Reviewed]

Genomic and proteomic characterization of the largeMyoviridaebacteriophage ϕTMA of the extreme thermophileThermus thermophilus
Masatada Tamakoshi; Aya Murakami; Motoki Sugisawa; Kenji Tsuneizumi; Shigeki Takeda; Toshihiko Saheki; Takashi Izumi; Toshihiko Akiba; Kaoru Mitsuoka; Hidehiro Toh; Atsushi Yamashita; Fumio Arisaka; Masahira Hattori; Tairo Oshima; Akihiko Yamagishi
Bacteriophage, May 2011, [Reviewed]

Prototype of frame-type cantilever for biosensor and femtogram detection
Hayato Sone; Shoichi Ichikawa; Yuji Matsubara; Mitsumasa Suzuki; Haruki Okano; Takashi Izumi; Sumio Hosaka
Key Engineering Materials, 2011, [Reviewed]

Oxidized free fatty acids as lipid mediators and functions of G2A as their receptor
Koji Kishimoto; Hideru Obinata; Mikiko Kishi; Noriyasu Ohshima; Kazuaki Tatei; Takashi Izumi
Seikagaku, 2011, [Reviewed]

Arachidonate 5-Lipoxygenase Establishes Adaptive Humoral Immunity by Controlling Primary B Cells and Their Cognate T-Cell Help
Tsutomu Nagashima; Shingo Ichimiya; Tomoki Kikuchi; Yoshiyuki Saito; Hiroshi Matsumiya; Shihoko Ara; Shigeru Koshiba; Jun Zhang; Chizuru Hatate; Akiko Tonooka; Terufumi Kubo; Rui Carrie Ye; Bungo Hirose; Hideaki Shirasaki; Takashi Izumi; Tsuyoshi Takami; Tetsuo Himi; Noriyuki Sato
AMERICAN JOURNAL OF PATHOLOGY, Jan. 2011, [Reviewed]

群馬大学における重粒子線がん治療
Oct. 2010

A Novel Glycerophosphodiester Phosphodiesterase, GDE5, Controls Skeletal Muscle Development via a Non-enzymatic Mechanism
Yuri Okazaki; Noriyasu Ohshima; Ikumi Yoshizawa; Yasutomi Kamei; Stefania Mariggio; Keiko Okamoto; Masahiro Maeda; Yoshihito Nogusa; Yuichiro Fujioka; Takashi Izumi; Yoshihiro Ogawa; Yoshitsugu Shiro; Masanobu Wada; Norihisa Kato; Daniela Corda; Noriyuki Yanaka
JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 2010, [Reviewed]

Cystathionine gamma-Lyase-deficient Mice Require Dietary Cysteine to Protect against Acute Lethal Myopathy and Oxidative Injury
Isao Ishii; Noriyuki Akahoshi; Hidenori Yamada; Shintaro Nakano; Takashi Izumi; Makoto Suematsu
JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 2010, [Reviewed]

Detection of G protein-coupled receptor-mediated cellular response involved in cytoskeletal rearrangement using surface plasmon resonance
Kexin Chen; Hideru Obinata; Takashi Izumi
BIOSENSORS & BIOELECTRONICS, Mar. 2010, [Reviewed]

Identification and Analysis of Two Splice Variants of Human G2A Generated by Alternative Splicing
Ai Ogawa; Hideru Obinata; Tomoyasu Hattori; Mikiko Kishi; Kazuaki Tatei; Osamu Ishikawa; Takashi Izumi
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, Feb. 2010, [Reviewed]

Detection of GPCR signals in living cell by SPR.　　　　　　　　　　　　　　　
K Chen; H Obinata; T Izumi
Biotech International, 2010

G2A as a receptor for oxidized free fatty acids
Hideru Obinata; Takashi Izumi
PROSTAGLANDINS & OTHER LIPID MEDIATORS, Sep. 2009, [Reviewed]

Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1
Tohko Hirano; Mikiko Kishi; Hiroyuki Sugimoto; Ryo Taguchi; Hideru Obinata; Noriyasu Ohshima; Kazuaki Tatei; Takashi Izumi
Biochim Biophys Acta- Molecular and Cell Biology of Lipids, Aug. 2009, [Reviewed]

Identification of nuclear localization and nuclear export signals in Ets2, and the transcriptional regulation of Ets2 and CTP:phosphocholine cytidylyltransferase alpha in tetradecanoyl-13-acetate or macrophage-colony stimulating factor stimulated RAW264 cells
Koichi Okamura; Satoko Yamashita; Hiromi Ando; Yasuhiro Horibata; Chieko Aoyama; Kenji Takagishi; Takashi Izumi; Dennis E. Vance; Hiroyuki Sugimoto
Biochim Biophys Acta- Molecular and Cell Biology of Lipids, Mar. 2009, [Reviewed]

Ceramide Induces Myogenic Differentiation and Apoptosis in Drosophila Schneider Cells
Hidemasa Kawamura; Kazuaki Tatei; Tetsuo Nonaka; Hideru Obinata; Tomoyasu Hattori; Ai Ogawa; Hideko Kazama; Nobuyuki Hamada; Tomoo Funayama; Tetsuya Sakashita; Yasuhiko Kobayashi; Takashi Nakano; Takashi Izumi
JOURNAL OF RADIATION RESEARCH, Mar. 2009, [Reviewed]

Identification of novel peptide agonists from a random peptide library for a 5-oxo-ETE receptor, a receptor for bioactive lipids
Yoshinori Sasaki; Takashi Tsujii; Shigeki Takeda; Hideru Obinata; Takashi Izumi; Keiichi Yamada; Ryoichi Katakai
Journal of Peptide Science, Dec. 2008, [Reviewed]

CDK5-dependent Phosphorylation of the Rho Family GTPase TC10 alpha Regulates Insulin-stimulated GLUT4 Translocation
Shuichi Okada; Eijiro Yamada; Tsugumichi Saito; Kihachi Ohshima; Koshi Hashimoto; Masanobu Yamada; Yutaka Uehara; Takafumi Tsuchiya; Hiroyuki Shimizu; Kazuaki Tatei; Takashi Izumi; Keishi Yamauchi; Shin-ichi Hisanaga; Jeffrey E. Pessin; Masatomo Mori
JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2008, [Reviewed]

キーワード解説 PAF受容体　　　　　　　　　　　　　　　
Dec. 2008

Genetic background conversion ameliorates semi-lethality and permits behavioral analyses in cystathionine beta-synthase-deficient mice, an animal model for hyperhomocysteinemia
Noriyuki Akahoshi; Chiho Kobayashi; Yasuki Ishizaki; Takashi Izumi; Toshiyuki Himi; Makoto Suematsu; Isao Ishii
HUMAN MOLECULAR GENETICS, Jul. 2008, [Reviewed]

Proteomic analysis identifies proteins that continue to grow hepatic stem-like cells without differentiation
Toshihiko Saheki; Hitomi Ito; Akihiro Sekiguchi; Atsuyoshi Nishina; Toshihiro Sugiyama; Takashi Izumi; Itaru Kojima
CYTOTECHNOLOGY, Jun. 2008, [Reviewed]

G2A plays proinflammatory roles in human keratinocytes under oxidative stress as a receptor for 9-hydroxyoctadecadienoic acid
Tomoyasu Hattori; Hideru Obinata; Ai Ogawa; Mikiko Kishi; Kazuaki Tatei; Osamu Ishikawa; Takashi Izumi
JOURNAL OF INVESTIGATIVE DERMATOLOGY, May 2008, [Reviewed]

プロトン感知性GPCR研究の展開　　　　　　　　　　　　　　　
Feb. 2008

The CNS abnormality in adult cystathionine beta-synthase-deficient mice
Noriyuki Akahoshi; Chiho Kobayashi; Yasuki Ishizaki; Takashi Izumi; Toshiyuki Himi; Makoto Suematsu; Isao Ishii
NEUROSCIENCE RESEARCH, 2008, [Reviewed]

Morphine is an exogenous ligand for MrgX2, a G protein-coupled receptor for cortistatin
Natsuyo Akuzawa; Hideru Obinata; Takashi Izumi; Shigeki Takeda
Journal of Cell and Animal Biology, 2008, [Reviewed]

オーファン受容体のリガンド探索 新たな生命現象の理解と創薬を目指して　　　　　　　　　　　　　　　
Mar. 2007

Purification and characterization of lysophospholipase D from rat brain
Sayaka Sugimoto; Hiroyuki Sugimoto; Chieko Aoyama; Chizu Aso; Masatomo Mori; Takashi Izumi
Biochim Biophys Acta -Molecular and Cell Biology of Lipids-, Dec. 2006, [Reviewed]

Differential induction of cystathionine gamma-lyase in the livers and kidneys of mouse dams during gestation and lactation
Noriyuki Akahoshi; Takashi Izumi; Yasuki Ishizaki; Isao Ishii
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Sep. 2006, [Reviewed]

Possibility of a femtogram mass biosensor using a self-sensing cantilever
S Hosaka; T Chiyoma; A Ikeuchi; H Okano; H Sone; T Izumi
CURRENT APPLIED PHYSICS, Jun. 2006, [Reviewed]

Femtogram mass biosensor using self-sensing cantilever for allergy check
H Sone; A Ikeuchi; T Izumi; H Okano; S Hosaka
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, Mar. 2006, [Reviewed]

5-oxo-eicosatetraenoic acid-induced chemotaxis: Identification of a responsible receptor hGPCR48 and negative regulation by G protein G(12/13)
D Koike; H Obinata; A Yamamoto; S Takeda; H Komori; F Nara; T Izumi; T Haga
JOURNAL OF BIOCHEMISTRY, Mar. 2006, [Reviewed]

Ｇタンパク質共役型受容体の最新知見　　　　　　　　　　　　　　　
2006

Sp1 is a co-activator with Ets-1, and net is an important repressor of the transcription of CTP : Phosphocholine cytidylyltransferase alpha
H Sugimoto; K Okamura; S Sugimoto; M Satou; T Hattori; DE Vance; T Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2005, [Reviewed]

Stress-induced nuclear export of 5-lipoxygenase
H Hanaka; T Shimizu; T Izumi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Dec. 2005, [Reviewed]

Identification of 9-hydroxyoctadecadienoic acid and other oxidized free fatty acids as ligands of the G protein-coupled receptor G2A
H Obinata; T Hattori; S Nakane; K Tatei; T Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY, Dec. 2005, [Reviewed]

An atypical protein kinase C, PKC zeta, regulates human eosinophil effector functions
M Kato; T Yamaguchi; A Tachibana; M Suzuki; T Izumi; K Maruyama; Y Hayashi; H Kimura
IMMUNOLOGY, Oct. 2005, [Reviewed]

Short report: Lethal malaria in cytosolic phospholipase A(2)- and phospholipase A(2)IIA-deficient mice
S Ishikawa; N Uozumi; T Shiibashi; T Izumi; M Fukayama; T Shimizu; J Watanabe; S Nogami
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Jun. 2004, [Reviewed]

Effects of heavy-ion irradiation on the early embryo of Drosophila melanogaster　　　　　　　　　　　　　　　
TATEI KAZUAKI; IZUMI TAKASHI; OBINATA HIDERU; NONAKA TETUO; NAKANO TAKASHI
TIARA Annual Report 2004, 2004

Glucocorticoids up-regulate leukotriene B-4 receptor-1 expression during neutrophilic differentiation of HL-60 cells
H Obinata; T Yokomizo; T Shimizu; T Izumi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Sep. 2003, [Reviewed]

Actin assembly is a crucial factor for superoxide anion generation from adherent human eosinophils
M Suzuki; M Kato; H Hanaka; T Izumi; A Morikawa
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Jul. 2003, [Reviewed]

基礎医学から呼吸器へのメッセージ：アラキドン酸代謝物と呼吸器　　　　　　　　　　　　　　　
Jul. 2003

Identification of Ets-1 as an important transcriptional activator of CTP : phosphocholine cytidylyltransferase alpha in COS-7 cells and co-activation with transcriptional enhancer factor-4
H Sugimoto; S Sugimoto; K Tatei; H Obinata; M Bakovic; T Izumi; DE Vance
JOURNAL OF BIOLOGICAL CHEMISTRY, May 2003, [Reviewed]

ロイコトリエンと呼吸器疾患　　　　　　　　　　　　　　　
2003

Inhibition of protein kinases A and C demonstrates dual modes of response in human eosinophils stimulated with platelet-activating factor
T Takizawa; M Kato; H Kimura; M Suzuki; A Tachibana; H Obinata; T Izumi; K Tokuyama; A Morikawa
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Aug. 2002, [Reviewed]

Leukotriene receptors: Classification, gene expression, and signal transduction
T Izumi; T Yokomizo; H Obinata; H Ogasawara; T Shimizu
JOURNAL OF BIOCHEMISTRY, Jul. 2002, [Reviewed]

Characterization of mouse cysteinyl leukotriene receptors mCysLT(1) and mCysLT(2) - Differential pharmacological properties and tissue distribution
H Ogasawara; S Ishii; T Yokomizo; T Kakinuma; M Komine; K Tamaki; T Shimizu; T Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY, May 2002, [Reviewed]

Receptor-dependent metabolism of platelet-activating factor in murine macrophages
Noriyasu Ohshima; Satoshi Ishii; Takashi Izumi; Takao Shimizu
Journal of Biological Chemistry, 22 Mar. 2002, [Reviewed]

Nuclear-localization-signal-dependent and nuclear-export-signal-dependent mechanisms determine the localization of 5-lipoxygenase
H Hanaka; T Shimizu; T Izumi
BIOCHEMICAL JOURNAL, Feb. 2002, [Reviewed]

Intracellular localization of 5-lipoxygenase
H Hanaka; T Shimizu; T Izumi
OXYGEN AND LIFE: OXYGENASES, OXIDASE AND LIPID MEDIATORS (International Congress Series), 2002, [Reviewed]

Characterization of two distinct types of leukotriene B-4 receptor
T Shimizu; T Yokomizo; T Izumi
OXYGEN AND LIFE: OXYGENASES, OXIDASE AND LIPID MEDIATORS (International Congress Series), 2002, [Reviewed]

In vivo chemotaxis using CHO cells expressing human leukotriene B-4 receptor
T Yokomizo; E Noiri; T Izumi; T Shimizu
EICOSANOIDS AND OTHER BIOACTIVE LIPIDS IN CANCER, INFLAMMATION, AND RADIATION INJURY, 5, 2002, [Reviewed]

Immunohistochemical localization of guinea-pig leukotriene B-4 12-hydroxydehydrogenase/15-ketoprostagland in 13-reductase
T Yamamoto; T Yokomizo; A Nakao; T Izumi; T Shimizu
EUROPEAN JOURNAL OF BIOCHEMISTRY, Dec. 2001, [Reviewed]

Involvement of ERK and p38 MAP kinase in oxidative stress-induced phospholipase D activation in PC12 cells
Y Banno; S Wang; Y Ito; T Izumi; S Nakashima; T Shimizu; Y Nozawa
NEUROREPORT, Jul. 2001, [Reviewed]

Role of PAF in acute liver injury after extended hepatectomy - Overexpression of PAF receptor mRNA in Kupffer cells
S Mizuno; T Izumi; S Isaji
DIGESTIVE DISEASES AND SCIENCES, Jun. 2001, [Reviewed]

Co-expression of two LTB4 receptors in human mononuclear cells
Takehiko Yokomizo; Takashi Izumi; Takao Shimizu
Life Sciences, 06 Apr. 2001, [Reviewed]

Hydroxyeicosanoids bind to and activate the low affinity leukotriene B-4 receptor, BLT2
T Yokomizo; K Kato; H Hagiya; T Izumi; T Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 2001, [Reviewed]

【麻酔管理に関連する受容体の最新情報】 受容体の基礎知識と新たな展開　　　　　　　　　　　　　　　
Mar. 2001

LTB4受容体およびLTD4受容体 (プロスタグランジン研究の新展開) -- (第1部 受容体の構造と機能)
Feb. 2001

Localization and translocation of enzymes involved in leukotriene biosynthesis
Y Ide; H Hanaka; T Hirabayashi; T Shimizu; T Izumi
ADVANCES IN PROSTAGLANDIN AND LEUKOTRIENE RESEARCH: BASIC SCIENCE AND NEW CLINICAL APPLICATIONS, 2001, [Reviewed]

BLT2, a second leukotriene B-4 receptor
T Yokomizo; K Kato; K Terawaki; T Izumi; T Shimizu
ADVANCES IN PROSTAGLANDIN AND LEUKOTRIENE RESEARCH: BASIC SCIENCE AND NEW CLINICAL APPLICATIONS, 2001, [Reviewed]

Leukotriene B-4: Metabolism and signal transduction
T Yokomizo; T Izumi; T Shimizu
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Jan. 2001, [Reviewed]

Mammalian PSP24s (alpha and beta isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems
Y Kawasawa; K Kume; T Izumi; T Shimizu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2000, [Reviewed]

Brain-specific expression of novel G-protein-coupled receptors, with homologies to Xenopus PSP24 and human GPR45
Y Kawasawa; K Kume; S Nakade; H Haga; T Izumi; T Shimizu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Oct. 2000, [Reviewed]

Cell-specific transcriptional regulation of human leukotriene B-4 receptor gene
K Kato; T Yokomizo; T Izumi; T Shimizu
JOURNAL OF EXPERIMENTAL MEDICINE, Aug. 2000, [Reviewed]

A second leukotriene B-4 receptor, BLT2: A new therapeutic target in inflammation and immunological disorders
T Yokomizo; K Kato; K Terawaki; T Izumi; T Shimizu
JOURNAL OF EXPERIMENTAL MEDICINE, Aug. 2000, [Reviewed]

【脂質メディエーターの酵素・受容体】 ロイコトリエンの産生制御と受容体　　　　　　　　　　　　　　　
Aug. 2000

Acute lung injury by sepsis and acid aspiration: a key role for cytosolic phospholipase A(2)
T Nagase; N Uozumi; S Ishii; K Kume; T Izumi; Y Ouchi; T Shimizu
NATURE IMMUNOLOGY, Jul. 2000, [Reviewed]

Leukotriene B-4 receptor - Cloning and intracellular signaling
T Yokomizo; K Masuda; K Kato; A Toda; T Izumi; T Shimizu
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Feb. 2000, [Reviewed]

Leukotriene-B-4 receptor and signal transduction
T Shimizu; T Yokomizo; T Izumi
ADVANCES IN EICOSANOID RESEARCH, 2000, [Reviewed]

An in vivo approach showing the chemotactic activity of leukotriene B-4 in acute renal ischemic-reperfusion injury
E Noiri; T Yokomizo; A Nakao; T Izumi; T Fujita; S Kimura; T Shimizu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jan. 2000, [Reviewed]

呼吸器系の生物学 脂質メディエーターと細胞応答　　　　　　　　　　　　　　　
Jan. 2000

Platelet-activating factor mediates acid-induced lung injury in genetically engineered mice
T Nagase; S Ishii; K Kume; N Uozumi; T Izumi; Y Ouchi; T Shimizu
JOURNAL OF CLINICAL INVESTIGATION, Oct. 1999, [Reviewed]

Cloning and characterization of rat leukotriene B-4 receptor
A Toda; T Yokomizo; K Masuda; A Nakao; T Izumi; T Shimizu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Sep. 1999, [Reviewed]

cDNA cloning and characterization of guinea-pig leukotriene B-4 receptor
K Masuda; T Yokomizo; T Izumi; T Shimizu
BIOCHEMICAL JOURNAL, Aug. 1999, [Reviewed]

生理活性脂質 ロイコトリエン受容体
Jun. 1999

【喘息とロイコトリエン拮抗薬】 最近の知見 基礎から臨床へ
Jun. 1999

Aminopeptidase B is structurally related to leukotriene-A(4) hydrolase but is not a bifunctional enzyme with epoxide hydrolase activity
KM Fukasawa; K Fukasawa; M Harada; J Hirose; T Izumi; T Shimizu
BIOCHEMICAL JOURNAL, May 1999, [Reviewed]

ロイコトリエンD4受容体を介する細胞内情報伝達機構　　　　　　　　　　　　　　　
Apr. 1999

Characterization of the leukotriene B-4 receptor in porcine leukocytes - Separation and reconstitution with heterotrimeric GTP-binding proteins
T Igarashi; T Yokomizo; O Tsutsumi; Y Taketani; T Shimizu; T Izumi
EUROPEAN JOURNAL OF BIOCHEMISTRY, Jan. 1999, [Reviewed]

Molecular cloning and characterization of leukotriene B-4 receptor
T Izumi; T Yokomizo; T Igarashi; T Shimizu
EICOSANOIDS AND OTHER BIOACTIVE LIPIDS IN CANCER, IMFLAMMATION AND RADIATION INJURY, 4, 1999, [Reviewed]

【エイコサノイド】 エイコサノイド基礎研究の推移
Jan. 1999

【喘息の遺伝子】 アナフィラキシー反応と気道過敏性における細胞質型ホスホリパーゼA2の役割　　　　　　　　　　　　　　　
Oct. 1998

ロイコトリエン受容体
Oct. 1998

ロイコトリエンB4受容体のcDNAクローニング　　　　　　　　　　　　　　　
Aug. 1998

Leukotriene receptors and their signal transduction.
Takashi Izumi; Mitsunobu Hoshino; Takehiko Yokomizo
Ensho, Jul. 1998
Leukotrienes (LTs) are 5-lipoxygenase metabolites derived from arachidonic acid and play important roles in many inflammation and allergic disorders. They are classified to two groups, LTB₄ and peptide-LTs. Both groups are thought to show their biological activities through their cellsurface receptors, but no LT receptor had been purified to homogeneity or cDNA-cloned. Recently, we cloned a cDNA for a LTB₄ receptor from HL 60 cells. The open reading frame of the cDNA encodes a protein of 352 amino acids and is predicted to contain seven membrane-spanning domains. In CHO cells stably expressing the LTB₄ receptor, LTB₄ elicited many signal transductions such as increase in intracellular calcium, InsP₃ accumulation, and inhibition of adenylyl cyclase. The CHO cells revealed a marked chemotactic response toward nM order of LTB₄. Next, we analyzed the signal transduction mechanisms through LTD₄ receptors using human monocytic leukemia THP-1 cells. When these cells were stimulated with LTD₄, intracellular calcium concentration was increased and MAP kinase was activated severalfold. This activation was mediated a protein kinase Cα-Raf-1 dependent pathway. A chemotactic response of THP-1 cells toward LTD₄ was observed. LT receptors transduce these signals through both PTX-sensitive and-insensitive G proteins.

Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor - Role of cytoplasmic tail phosphorylation in each process
I Ishii; E Saito; T Izumi; M Ui; T Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Apr. 1998, [Reviewed]

Leukotriene D-4 activates mitogen-activated protein kinase through a protein kinase C alpha Raf-1-dependent pathway in human monocytic leukemia THP-1 cells
M Hoshino; T Izumi; T Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 1998, [Reviewed]

【脂質メディエーターと気管支喘息】 受容体の構造と機能 ロイコトリエン受容体,PAF受容体　　　　　　　　　　　　　　　
Nov. 1997

The molecular biology introduction series. 8. Protein small-amount purification and amino acid sequence determination.
Jul. 1997

A G-protein-coupled receptor for leukotriene B-4 that mediates chemotaxis
T Yokomizo; T Izumi; K Chang; Y Takuwa; T Shimizu
NATURE, Jun. 1997, [Reviewed]

Alanine exchanges of polar amino acids in the transmembrane domains of a platelet-activating factor receptor generate both constitutively active and inactive mutants
I Ishii; T Izumi; H Tsukamoto; H Umeyama; M Ui; T Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 1997, [Reviewed]

Comparison of platelet-activating factor receptor mRNA levels in peripheral blood eosinophils from normal subjects and atopic asthmatic patients
S Kishimoto; W Shimadzu; T Izumi; T Shimizu; H Sagara; T Fukuda; S Makino; T Sugiura; K Waku
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1997, [Reviewed]

cDNA cloning and mutagenesis study of leukotriene B-4 12-hydroxydehydrogenase
T Yokomizo; Y Ogawa; N Uozumi; K Kume; T Izumi; T Shimizu
EICOSANOIDS AND OTHER BIOACTIVE LIPIDS IN CANCER, INFLAMMATION, AND RADIATION INJURY 3, 1997, [Reviewed]

High and low affinity mutants of platelet-activating factor receptor
I Ishii; T Izumi; M Ui; T Shimizu
RECENT ADVANCES IN PROSTAGLANDIN, THROMBOXANE, AND LEUKOTRIENE RESEARCH, 1997, [Reviewed]

Cloning and characterization of a murine platelet-activating factor receptor gene
S Ishii; M Nakamura; Waga, I; K Kume; T Izumi; T Shimizu
EICOSANOIDS AND OTHER BIOACTIVE LIPIDS IN CANCER, INFLAMMATION, AND RADIATION INJURY 3, 1997, [Reviewed]

Regulation by IL-5 of expression of functional platelet-activating factor receptors on human eosinophils
S Kishimoto; W Shimadzu; T Izumi; T Shimizu; T Fukuda; S Makino; T Sugiura; K Waku
JOURNAL OF IMMUNOLOGY, Nov. 1996, [Reviewed]

PAFと炎症 PAFレセプターの情報伝達　　　　　　　　　　　　　　　
Oct. 1996

細胞内シグナル伝達 生理活性脂質のシグナル伝達系 血小板活性化因子(PAF)　　　　　　　　　　　　　　　
Aug. 1996

Exchanges of polar amino acids to alanine generate platelet-activating factor receptor mutants with altered affinities to their ligand.
I Ishii; T Izumi; M Ui; T Shimizu
FASEB JOURNAL, Apr. 1996

遺伝子の発現解析 遺伝子導入 リポフェクション　　　　　　　　　　　　　　　
Apr. 1996

A murine platelet-activating factor receptor gene: Cloning, chromosomal localization and up-regulation of expression by lipopolysaccharide in peritoneal resident macrophages
S Ishii; Y Matsuda; M Nakamura; Waga, I; K Kume; T Izumi; T Shimizu
BIOCHEMICAL JOURNAL, Mar. 1996, [Reviewed]

cDNA cloning, expression, and mutagenesis study of leukotriene B-4 12-hydroxydehydrogenase
T Yokomizo; Y Ogawa; N Uozumi; K Kume; T Izumi; T Shimizu
JOURNAL OF BIOLOGICAL CHEMISTRY, Feb. 1996, [Reviewed]

PAF受容体　　　　　　　　　　　　　　　
1996

Enhanced expression of platelet-activating factor receptor on human eosinophils by interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor
S Kishimoto; W Shimazu; T Izumi; T Shimizu; T Fukuda; S Makino; T Sugiura; K Waku
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1996, [Reviewed]

GTP結合蛋白質と連関する細胞膜受容体 PAF受容体
Jan. 1996

Platelet-activating factor receptor: Gene expression and signal transduction
T Izumi; T Shimizu
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, Dec. 1995, [Reviewed]

Leukotriene A4 hydrolase and leukotriene B4 metabolism
Takehiko Yokomizo; Naonori Uozumi; Toshie Takahashi; Kazuhiko Kume; Takashi Izumi; Takao Shimizu
Journal of Lipid Mediators and Cell Signalling, Oct. 1995, [Reviewed]

Platelet-activating factor receptor
Takashi Izumi; Tomoko Takano; Haruhiko Bito; Motonao Nakamura; Hiroyuki Mutoh; Zen-ichiro Honda; Takao Shinizu
Journal of Lipid Mediators and Cell Signalling, Oct. 1995, [Reviewed]

エイコサノイドとPAFの生理的作用　　　　　　　　　　　　　　　
Oct. 1995

New enzyme activity of leukotriene A4 hydrolase.
May 1995

Expression of human platelet-activating factor receptor gene in EoL-1 cells following butyrate-induced differentiation
T Izumi; S Kishimoto; T Takano; M Nakamura; Y Miyabe; M Nakata; C Sakanaka; T Shimizu
Biochemical Journal, 01 Feb. 1995, [Reviewed]

Regulation and signal-transduction of PAF receptor
T IZUMI; Z HONDA; H MUTOH; K KUME; T SHIMIZU
PROSTAGLANDINS AND RELATED COMPOUNDS, 1995, [Reviewed]

Platelet-Activating Factor (PAF) Positively Auto-Regulates the Expression of Human PAF Receptor Transcript 1 (Leukocyte-Type) Through NF-κB
H Mutoh; S Ishii; T Izumi; S Kato; T Shimizu
Biochemical and Biophysical Research Communications, Dec. 1994, [Reviewed]

Activation of Mitogen‐Activated Protein Kinase and Arachidonate Release via Two G Protein‐Coupled Receptors Expressed in the Rat Hippocampus^a
MIKIRO MORI; HARUHIKO BITO; CHIE SAKANAKA; ZEN‐ICHIRO HONDA; KAZUHIKO KUME; TAKASHI IZUMI; TAKAO SHIMIZU
Annals of the New York Academy of Sciences, Nov. 1994

Role of cytoplasmic tail phosphorylation sites of platelet-activating factor receptor in agonist-induced desensitization
T TAKANO; Z HONDA; C SAKANAKA; T IZUMI; K KAMEYAMA; K HAGA; T HAGA; K KUROKAWA; T SHIMIZU
JOURNAL OF BIOLOGICAL CHEMISTRY, Sep. 1994, [Reviewed]

Crystallization and Preliminary X-ray Crystallographic Studies of Recombinant Human Leukotriene A4 Hydrolase Complexed with Bestatin
Hideaki Tsuge; Hideo Ago; Michiko Aoki; Masahiro Furuno; Masana Noma; Masashi Miyano; Michiko Minami; Takashi Izumi; Takao Shimizu
Journal of Molecular Biology, May 1994, [Reviewed]

血小板活性化因子による生体制御 PAF受容体のクローニングと情報伝達　　　　　　　　　　　　　　　
Apr. 1994

Structure and function of platelet-activating-factor receptor
T SHIMIZU; H MUTOH; WAGA, I; M NAKAMURA; Z HONDA; T IZUMI
MOLECULAR BASIS OF INFLAMMATION, 1994, [Reviewed], [Invited]

Enzymatic inactivation of leukotriene B4 by a novel enzyme found in the porcine kidney. Purification and properties of leukotriene B4 12-hydroxydehydrogenase
T YOKOMIZO; T IZUMI; T TAKAHASHI; T KASAMA; Y KOBAYASHI; F SATO; Y TAKETANI; T SHIMIZU
JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 1993, [Reviewed]

Energy-dependent export of leukotriene B4 in Xenopus oocytes
Mikiro Mori; Takashi Izumi; Takao Shimizu
Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 20 May 1993, [Reviewed]

Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor
Hiroyuki Mutoh; Haruhiko Bito; Michiko Minami; Motonao Nakamura; Zenichiro Honda; Takashi Izumi; Ryo Nakata; Yoshihisa Kurachi; Akira Terano; Takao Shimizu
FEBS Letters, 10 May 1993

Site-directed mutagenesis of leukotriene-A(4) hydrolase - distinction of leukotriene-A(4) hydrolase and aminopeptidase activities
T IZUMI; M MINAMI; N OHISHI; H BITO; T SHIMIZU
JOURNAL OF LIPID MEDIATORS, Mar. 1993, [Reviewed]

アラキドン酸代謝情報伝達解析法 アラキドン酸代謝測定法(1)リポキシゲナーゼ系　　　　　　　　　　　　　　　
Feb. 1993

Three types of Giα protein of the guinea-pig lung: cDNA cloning and analysis of their tissue distribution
Chie Sakanaka; Takashi Izumi; Motonao Nakamura; Zen-ichiro Honda; Tsuyoshi Watanabe; Michiko Minami; Hiroyuki Mutoh; Haruhiko Bito; Yousuke Seyama; Michio Ui; Takao Shimizu
BBA - Molecular Cell Research, 15 Dec. 1992, [Reviewed]

Leukotriene A₄hydrolase, a bifunctional enzyme Distinction of leukotriene A₄hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297
Michiko Minami; Haruhiko Bito; Nobuya Ohishi; Hideaki Tsuge; Masashi Miyano; Mikiro Mori; Hiroo Wada; Hiroyuki Mutoh; Shinobu Shimada; Takashi Izumi; Keiko Abe; Takao Shimizu
FEBS Letters, 14 Sep. 1992, [Reviewed]

Platelet-activating factor receptor and signal transduction
Shimizu Takao; Honda Zenichiro; Nakamura Motonao; Bito Haruhiko; Izumi Takashi
Biochemical Pharmacology, Sep. 1992, [Reviewed]

Platelet-activating factor (PAF) receptor in rat brain: PAF mobilizes intracellular Ca2+ in hippocampal neurons
Haruhiko Bito; Motonao Nakamura; Zenichiro Honda; Takashi Izumi; Takeshi Iwatsubo; Yousuke Seyama; Akihiko Ogura; Yoshihisa Kudo; Takao Shimizu
Neuron, Aug. 1992, [Reviewed]

Biosynthesis of leukotriene C(4)
T IZUMI; T SHIMIZU
FRONTIERS AND NEW HORIZONS IN AMINO ACID RESEARCH, 1992, [Reviewed]

Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes
Takashi IZUMI; Olof RADMARK; Hans JORNVALL; Bengt SAMUELSSON
European Journal of Biochemistry, Dec. 1991, [Reviewed]

Molecular cloning and expression of platelet-activating factor receptor from human leukocytes.
M Nakamura; Z Honda; T Izumi; C Sakanaka; H Mutoh; M Minami; H Bito; Y Seyama; T Matsumoto; M Noma; T Shimizu
Journal of Biological Chemistry, Oct. 1991, [Reviewed]

情報伝達系基礎実験法 アラキドン酸代謝情報伝達解析法 アラキドン酸代謝測定法(2) リポキシゲナーゼ系　　　　　　　　　　　　　　　
May 1991

アラキドン酸リポキシゲナーゼファミリーについて
Mar. 1991

Purification of 15-lipoxygenase from human-leukocytes, evidence for the presence of isozymes
T IZUMI; O RADMARK; B SAMUELSSON
ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH, 1991, [Reviewed]

アラキドン酸5リ‐ポキシゲナーゼ活性化蛋白(FLAP)
Jan. 1991

Leukotriene A4 hydrolase is a zinc-containing aminopeptidase
Michiko Minami; Nobuya Ohishi; Hiroyuki Mutoh; Takashi Izumi; Haruhiko Bito; Hiroo Wada; Yousuke Seyama; Hiroyuki Toh; Takao Shimizu
Biochemical and Biophysical Research Communications, Dec. 1990, [Reviewed]

Cloning of the cDNA for human 12-lipoxygenase.
T Izumi; S Hoshiko; O Rådmark; B Samuelsson
Proceedings of the National Academy of Sciences, Oct. 1990, [Reviewed]

Biosynthesis and functions of leukotriene C4
T SHIMIZU; T IZUMI; Z HONDA; Y SEYAMA; Y KURACHI; T SUGIMOTO
ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH, 1990, [Reviewed]

Purification and characterization of human lung leukotriene A4 hydrolase
Nobuya Ohishi; Takashi Izumi; Yousuke Seyama; Takao Shimizu
Methods in Enzymology, 1990

Potato arachidonate 5-lipoxygenase: Purification, characterization, and preparation of 5(S)-hydroperoxyeicosatetraenoic acid
Takao Shimizu; Zen-Ichiro Honda; Ichiro Miki; Yousuke Seyama; Takashi Izumi; Olof Rådmark; Bengt Samuelsson
Methods in Enzymology, 1990

15-リポキシゲナーゼ(ω-6-リポキシゲナーゼ)cDNAのクローニング　　　　　　　　　　　　　　　
Nov. 1989

Native and mutant 5-lipoxygenase expression in a baculovirus/insect cell system.
C D Funk; H Gunne; H Steiner; T Izumi; B Samuelsson
Proceedings of the National Academy of Sciences, Apr. 1989, [Reviewed]

Partial-purification and characterization of leukotriene C4 synthase from guinea-pig lung
T IZUMI; Z HONDA; N OHISHI; S KITAMURA; Y SEYAMA; T SHIMIZU
ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH, 1989, [Reviewed]

Effects of Smoking on the Synthesis of Leukotriene B₄ by Rat Alveolar Macrophages
Yukihiko Sugiyama; Takashi Izumi; Nobuya Ohishi; Hideki Yotsumoto; Takao Shimizu; Satoshi Kitamura; Fumimaro Takaku
Respiration, 1989, [Reviewed]

Synthesis and structural identification of four dihydroxy acids and 11, 12-leukotriene C4 derived from 11, 12-leukotriene A4
Shigeto KITAMURA; Takao SHIMIZU; Ichiro MIKI; Takashi IZUMI; Takeshi KASAMA; Akira SATO; Hiroshi SANO; Yousuke SEYAMA
European Journal of Biochemistry, Oct. 1988, [Reviewed]

質量分析法の新展開 生理活性物質(プロスタグランジン,ロイコトリエン)
Sep. 1988

Solubilization and partial purification of leukotriene C4 synthase from guinea-pig lung: a microsomal enzyme with high specificity towards 5,6-epoxide leukotriene A4
T IZUMI; Z HONDA; N OHISHI; S KITAMURA; S TSUCHIDA; K SATO; T SHIMIZU; Y SEYAMA
BIOCHIMICA ET BIOPHYSICA ACTA, Apr. 1988, [Reviewed]

Purification of a new acidic glutathione S-transferase, GST-Yn1 Yn1, with a high leukotriene-C4 synthase activity from rat brain
Shigeki TSUCHIDA; Takashi IZUMI; Takao SHIMIZU; Takashi ISHIKAWA; Ichiro HATAYAMA; Kimihiko SATOH; Kiyomi SATO
European Journal of Biochemistry, Dec. 1987, [Reviewed]

Enzymic Synthesis of Leukotriene B₄in Guinea Pig Brain
Takao Shimizu; Yutaka Takusagawa; Takashi Izumi; Nobuya Ohishi; Yousuke Seyama
Journal of Neurochemistry, May 1987, [Reviewed]

Synthesis of 11,12-leukotriene A₄, 11S,12S-oxido-5Z,7E,9E,14Z-eicosatetraenoic acid, a novel leukotriene of the 12-lipoxy genase pathway
Shigeto Kitamura; Takao Shimizu; Takashi Izumi; Yousuke Seyama
FEBS Letters, 09 Mar. 1987, [Reviewed]

ガスクロマトグラフィー・マススペクトロメトリー(GC-MS)法によるロイコトリエンB4およびω-代謝産物の同時微量測定法　　　　　　　　　　　　　　　
Feb. 1987

ロイコトリエンB４の微量定量と生合成に関する研究　　　　　　　　　　　　　　　
1987

Biosynthesis and further transformations of leukotriene A4
T Shimizu; T Izumi; N Ohishi; Y Seyama; S Kitamura
Adv Prostaglandin Thromboxane Leukot Res., 1987, [Reviewed]

Leukotriene A4 hydrolase in the human lung. Inactivation of the enzyme with leukotriene A4 isomers
N Ohishi; T Izumi; M Minami; S Kitamura; Y Seyama; S Ohkawa; S Terao; H Yotsumoto; F Takaku; T Shimizu
Journal of Biological Chemistry, 1987, [Reviewed]

Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase.
T Shimizu; T Izumi; Y Seyama; K Tadokoro; O Rådmark; B Samuelsson
Proceedings of the National Academy of Sciences, Jun. 1986, [Reviewed]

アラキドン酸カスケードと肺
Jun. 1986

Tissue distribution of leukotriene A4 hydrolase activity in guinea pig
Takashi Izumi; Takao Shimizu; Yousuke Seyama; Nobuya Ohishi; Fumimaro Takaku
Biochemical and Biophysical Research Communications, Feb. 1986, [Reviewed]

プロスタグランジン,ロイコトリエン研究の最近の進歩 呼吸器系を中心に　　　　　　　　　　　　　　　
Jan. 1986

A simultaneous quantitation of leukotriene B4 and its ω-oxidized products by gas chromatography-mass spectrometry
Takashi Izumi; Takao Shimizu; Takeshi Kasama; Yousuke Seyama; Hideki Sumimoto; Koichiro Takeshige; Shigeki Minakami; Anders Wetterholm; Olof Rådmark
Biochemical and Biophysical Research Communications, Jan. 1986, [Reviewed]

Intravascular bronchiolo-alveolar tumor (IVBAT) - Case report with the immunological analysis.
Yukihiko SUGIYAMA; Kazuyoshi YAMAGUCHI; Teruaki OKA; Takashi IZUMI; Satoshi KITAMURA; Fumimaro TAKAKU
Japanese Journal of Medicine, 1986, [Reviewed]

下気道疾患症例に対するBerachinの使用経験
Sep. 1984

過換気症候群
Jun. 1984

びまん性汎細気管支炎およびその他の呼吸器疾患における寒冷凝集素価の検討　　　　　　　　　　　　　　　
May 1984

Three Autopsied Cases of Generalized Mucormycosis with Lung Involvement Complicating Lymphoma and Leukemia
Yukihiko Sugiyama; Takashi Izumi; Kenji Sasaki; Satoshi Kitamura; Fumimaro Takaku; Kazuyoshi Yamaguchi
THE JAPANESE JOURNAL OF THORACIC DISEASES, Mar. 1984
Pulmonary involvement with mucormycosis is quite uncommon, but the incidence has been increasingly recognized, especially in patients with leukemia or lymphoma. We report three autopsied cases of generalized mucormycosis (phycomycosis) in patients with non-Hodgkin's lymphoma, acute exacerbation of chronic myelogenous leukemia, and acute myelogenous leukemia. All patients had abnormal lung shadows and high fever unresponsive to broad-spectrum antibiotics and trimethoprim-sulfamethoxazole.
Their chest X-ray findings were as follows;
Cases 1: Diffuse ground glass appearance with nodular density at the right hulim suggesting hemorrhagic infarction.
Cases 2: Diffuse ground glass appearance.
Cases 3: Several patchy infiltrates in the right lung and a large nodular density with cavities in the left lung field.
In two of three cases, flucytosine was administered without any remarkable effect. Histological examination revealed multiple infarction and thrombosis in various parts of pulmonary arteries in which broad and nonseptate hyphae were found. Sputum cultures were performed in all cases white they were alive, but no pathognomonic organism was found. Premortal diagnosis of mucormycosis is extremely difficult, but one possible way of diagnosis might be by lung perfusion scanning using ¹³¹I-MAA.

A Case of Sino-Bronchiectasis Associated with Lack of HLA A, B, and C Antigens.
Yukihiko Sugiyama; Takashi Izumi; Satoshi Kitamura; Fumimaro Takaku; Hiroo Maeda
THE JAPANESE JOURNAL OF THORACIC DISEASES, 1984
We present a case of diffuse sino-bronchiectasis associated with the lack of expression of HLA A, B, and C antigens. The case was a 40-year-old male diagnosed in our outpatient clinic as sino-bronchiectasis at about ago 20. In these 20 years, bronchiectasis has progressed gradually. Twenty years ago, bronchiectasis was limited to bilateral middle lobe bronchi, but now it extends to all bronchi.
In this patients, we also found the lack of expression of HLA A, B, and C antigens on his lymphocytes. Concerning other immunological data, his serum IgA, α₁-antitrypsin, and β₂-microglobulin levels were increased, and serum IgG, IgM and complements levels were within normal limits. Lymphocyte stimulation tests using, PHA, PWM, and Con-A show slightly low but positive reactions. Also, in the analysis of lymphocyte subsets the Leu-3aILeu-2a ratio showed a slight increase. It has been suggested that HLA molecules play an important role in immunological cellular interactions, especially between T-lymphocytes and other cells.
Therefore, we suspect that disorders of surface antigens on the lymphocytes of this case could be closely related to the respiratory tract disorders.

胸腺原発が疑われた Squamous cell carcinoma の１例　　　　　　　　　　　　　　　
Dec. 1983

Effect of Pentobarbital Sodium on Tracheal Pressure Increase Induced by Vagus Nerve Stimulation in Anesthetized Dogs.
S Kitamura; Y Ishihara; Y Sugiuama; T Izumi; R Hayashi; E Kyo; F Takaku
THE JAPANESE JOURNAL OF THORACIC DISEASES, Dec. 1983, [Reviewed]
It has been demonstrated that many different bronchoactive agents can cause a vagally mediated bronchoconstriction in animals and in man. Although the effects of local anesthetic aerosols on reflex bronchoconstriction have been investigated in the dog, little is known about the effects of intravenous general anesthetic commonly used in animal experiments on the reflex component of vagus nerve-induced bronchoconstriction.
We anesthetized 32 young mongrel dogs, weighing 6 to 10kg, with chloralose (30mg/kg), paralyzed the respiratory muscle with succinylcholine chloride, and ventilated the lungs through a tracheal cannula at a constant tidal volume (150-250ml) and a frequency of 16/min. Catheters were inserted into the left femoral artery and vein, and measurement of systemic arterial blood pressure (Psyst) and transfusion or infusion of drugs were conducted, respectively. The pressure transducer catheter was connected to the orifice of the tracheal cannula to monitor tracheal pressure (Ptr). The right side vagosympathetic nerve (V.N.) was isolated at the level of thyroid cartilage. We stimulated the cervical V.N. electrically with 5 to 30C from a square-wave stimulator (ME-6052); pulse duration was 1ms with a frequency of 30shocks/s.
1) The percent change of Ptr showed a marked increase by increasing stimulation voltage.
2) The percent increase of Ptr induced by V.N. stimulation showed a dose-dependent decrease with intravenous administration of pentobarbital sodium and ketamine hydrochloride.
3) The percent increase of Ptr suppressed by an intravenous infusion of pentobarbital sodium and ketamine hydrochloride showed a dose-dependent recovery by intravenous administration of neostigmine bromide.
4) The percent increase of Ptr potentiated by neostigmine bromide was suppressed dose-dependently by intravenous administration of pentobarbital sodium.
5) The percent increase of Ptr induced by an intravenous administration of acetylcholine and prostaglandin F_2α did not show any change by administration of pentobarbital sodium.

皮膚病変,表在リンパ節腫脹,肝脾腫,高γ-グロブリン血症を伴ったLymphoid Interstitial Pneumoniaの一例
Nov. 1983

亜急性肺性心を呈した肺腫瘍塞栓症の１剖検例
Sep. 1983

びまん性汎細気管支炎の内視鏡像と中枢気道粘膜組織像
Aug. 1983

Local Administration of Thrombin Solution for the Treatment of Bleeding Induced by Transbronchial Biopsy
The Journal of the Japan Society for Respiratory Endoscopy, Jun. 1983
Transbronchial lung biopsy (TBLB) is used for the diagnosis of pulmonary nodules and diffuse lung disease. Reports of complications have been infrequent, but deaths from hemorrhage have been reported. In the present investigation patients who suffered from hemorrhage induced by TBLB, bronchial mucous membrane biopsy and from local injection of anticancer drugs were treated with thrombin solution. Hemorrhage (30-200ml) occurred in 12 of 122 tumor biopsy cases, 14 of 170 TBLB cases, 2 of 102 local anticancer drug injection procedures and 1 of 20 bronchial mucous membrane biopsy cases. For these 29 cases of hemorrhage, we instilled 5 ml of thrombin solution (100 U/ml). In 27 patients, hemorrhage stopped after instillation of one dose of thrombin, and in the 2 other patients stopped after a second dose within 2 minutes. The avove results suggest that thrombin solution instillation therapy might be a useful method to treat hemorrhage induced by the various fiberoptic procedures.

ホルモン療法などが奏効した肺癌性リンパ管炎(乳癌原発)の1症例
北村 諭; 杉山 幸比古; 和泉 孝志
日本胸部臨床, May 1983

喀痰よりPasteurella ureaeが検出された慢性気管支炎の1症例
Apr. 1983

肺病変を合併したSjoegren症候群の2例
Apr. 1983

縦隔リンパ節腫脹,胸水,腹水,皮疹など多彩な症状を呈した結核の1治験例
Mar. 1983

Effect of leukotriene C4 and D4 on rabbit aorta and pulmonary artery strips
Mar. 1983, [Reviewed]

Effect of Various Drugs on Bronchoconstriction Induced by Vagosympathetic Nerve Stimulation
Kitamura Satoshi; Ishihara Yoko; Izumi Takashi; Hsu Lung-Hung
The Japanese journal of thoracic diseases, Feb. 1983, [Reviewed]
Bronchial hyperreactivity to nonspecific bronchoconstrictor stimuli is one of the diagnostic features of asthma. In the present investigation we attempted to determine whether various bronchoactive agents act on bronchoconstriction induced by vagosympathetic nerve stimulation.
We anesthetized 32 young mongrel dogs, weighing 7 to 10kg, with sodium pentobarbital (25mg/kg), paralyzed the respiratory muscles with succinylcholine, and ventilated the lungs through a tracheal cannula at a constant tidal volume (150-250ml) and a frequency of 16/min. Catheters were inserted into the left femoral artery and vein, and measurement of systemic arterial blood pressure (Psyst) and transfusion or infusion of drugs were conducted, respectively. The pressure transducer catheter was connected to the orifice of the tracheal cannula to monitor tracheal pressure (Ptr). The right side vagosympathetic nerve was isolated at the level of thyroid cartilage. We stimulated the cervical vagosympathetic nerve electrically with 5-25V from a square-wave stimulator (ME-6052); pulse duration was lms and the frequencies were 30shocks/s.
1) Percent change of Ptr showed a maked increase by increasing stimulation voltage and reached a plateau at 15 V.
2) Percent change of Ptr induced by vagosympathetic nerve stimulation showed a marked increase 5min after intravenous infusion of neostigmine bromide, and thereafter it showed a gradual decrease and returned to the control level after 75min.
3) Percent change of Ptr induced by vagosympathetic nerve stimulation showed a significant increase with continuous infusion of PGF_2α, serotonin, histamine, Tx B₂, LT C₄ and LT D₄.
4) Percent change of Ptr induced by vagosympathetic nerve stimulation showed a significant decrease with continuous infusion of isoproterenol, PGE₂, 6-keto PGF_1α and aminophylline.
5) Percent change of Ptr induced by vagosympathetic nerve stimulation ahowed a marked increase after pretreatment with propranolol dose-dependently, and reached a plateau at the dose of 600μg/kg.
6) Percent change of Ptr induced by vagosympathetic nerve stimulation showed a significant increase after pretreatment with propranolol and showed a significant decrease after pretreatment with phenoxybenzamine.
The above results suggest that bronchoconstrictor responses induced by vagosympathetic nerve stimulation were modulated by various vasoactive substances.

術後１４年で肺転移をきた腎腫瘍 clear cell carcinoma の一剖検例
Feb. 1983

老年者の気管支喘息に対するHC20-511 (ketotifen) の臨床効果　　　　　　　　　　　　　　　
Jan. 1983

A case of lymphoid interstitial pneumonia accompanied with skin eruption, generalized lymph adenopathy, polyclonal hypergamma-globulinemia and hepatosplenomegaly
Y. Sugiyama; T. Izumi; S. Kitamura; F. Takaku; K. Yamaguchi
Japanese Journal of Thoracic Diseases, 1983, [Reviewed]

An autopsied case of pulmonary tumor embolization presenting a clinical picture of subacute cor pulmonale
L. H. Hsu; T. Izumi; Y. Sugiyama; S. Kitamura; F. Takaku; K. Yamaguchi
Japanese Journal of Thoracic Diseases, 1983, [Reviewed]

一卵性双生児の一方に下垂体性巨人症を,他方に先端巨大症を発症した兄弟例
Nov. 1982, [Reviewed]

A Case with Diffuse Hemangiomas in the Mediastinum and in the Lung
IZUMI Takashi; KITAMURA Satoshi; SUGIYAMA Yukihiko; HAYASHI Ryujiro; HSU Lung Hung
The Journal of the Japan Society for Respiratory Endoscopy, Oct. 1982
An 18 year-old man was admitted to our hospital complaining of a small amount of bloody sputum and abnormal chest roentgenograms. He had several congenital skin angiomas. The colours of these angiomas were reddish purple, and turned pale on compression. Laboratory examinations showed almost normal values, but blood gas analysis revealed mild hypoxemia. Chest roentgenograms showed mediastinal widening and diffuse reticulo-nodular shadows throughout both lungs. These findings on chest radiograms had been recognized since he was 6years old, but the etiology had not been elucidated. Bronchoscopy demonstrated telangiectasis in bronchial mucosa and soft masses like varices along the left main bronchus. Pulmonary angiograpy showed narrowing of the left main pulmonary artery. Bronchial arteriography revealed elongation and dilatation of both bronchial arteries and early filling of the contrast media into the mediastinal and pulmonary masses. The main draining vessels were pulmonary veins. These findings were compatible with diffuse hemangiomas in the mediastinum and in the lung.

胸部X線像の読み方(VIII)肺好酸球性肉芽腫症(肺histiocytosisX)
Aug. 1982

慢性気管支炎患者に対するIpratropium bromide(Sch1000)の使用経験
May 1982

肺における血管作動性物質の作用におよぼす気管支動脈結紮の効果について
May 1982, [Reviewed]

Direct β‐Adrenergic Receptor Assay of Guinea Pig Lung :Effects of α, β-Adrenergic Receptor Blockers
Izumi Takashi; Kitamura Satoshi; Kosaka Kinori
The Japanese journal of thoracic diseases, May 1982, [Reviewed]
The lung is one of the organs that possess abundant β-adrenergic receptors. Direct radiologic binding methods for studying β-adrenergic receptors have been developed recently. These methods can provide new insights into the molecular characteristics and regulatory properties of β-adrenergic receptors in the lung.
Recently, α-adrenergic receptor blockers have been used for the therapy of brochial asthma and some good theraeputic effects have been reported. However the mechanisms have not been elucidated yet.
β-adrenergic receptor blockers are widely used for patients with hypertension or ischemic heart diseases, and sometimes the use of β-adrenergic receptor blockers elicits asthmatic attacks in patients with bronchial asthma. But actually the incidence of such attacks is not so high as could be expected.
In the present investigation we determined the number of β-adrenergic receptors in the lung membrane of guinea-pigs, weighing 150-280g. by direct methods using [³H]-(-)-dihydroalprenolol ([³H]-DHA). Effects of phentolamine and propranolol on the β-adrenergic receptors in the lung were also investigated.
1) The mean values and standard deviation of the number of β-adrenergic receptors (the concentration of [³H]-DHA binding sites) and the dissociation constant (Kd) were 789±121f mol/mg protein and 1.14±0.22nM, respectively (n=10).
2) The treatment of guinea-pigs with phentolamine (1mg/kg, subcutaneously, for 6 days) induced a significant increase in the number of lung β-adrenergic receptors, 971±149fmol/mg protein (n=8, p<0.02), while Kd did not show any significant change. This increase in the number of lung β-adrenergic receptors might be one of the mechanisms of α- adrenergic receptor blockers in bronchial asthma.
3) The treatment of guinea-pigs with propranolol (1mg/kg, subcutaneously, for 6 days) induced a significant increase in the number of lung β-adrenergic receptors, 931±141fmol/mg protein (n=8, p<0.05), while Kd did not show any significant change. This increase in the number of lung β-adrenergic receptors might be one of the reasons why the incidence of asthmatic attacks is not so high in spite of the widespread use of β-adrenergic receptor blockers.

頸部・縦隔リンパ節腫脹, 胸・腹水貯留, 皮疹など多彩な症状を呈した結核の1症例　　　　　　　　　　　　　　　
Apr. 1982

Coin lesion の鑑別診断をめぐって　　　　　　　　　　　　　　　
Apr. 1982

後縦隔腫瘍状陰影を呈した肋骨原発軟骨肉腫の一手術例
Apr. 1982

強制的噴門拡張療法にて著効を認めたVigorous achalasiaの1症例
Mar. 1982

吸引性肺炎の病態に関する実験的研究
Mar. 1982, [Reviewed]

粟粒結核の診断における経気管支肺生検の有用性について
Feb. 1982

経過中に糖尿病および肝膿瘍を合併したバーター症候群の１例　　　　　　　　　　　　　　　
1982

Effect of Ipratropium Bromide on the action of bronchoactive agents
S KITAMURA; Y ISHIHARA; Y SUGIYAMA; T IZUMI; R HAYASHI; LH HSU; K KOSAKA
ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH, 1982, [Reviewed]

多発性肺軟骨腫の手術例
Oct. 1981

胆嚢原発のＡＤＨ産生腫瘍　　　　　　　　　　　　　　　
Sep. 1981

自然気胸における血清アンジオテンシン変換酵素活性の検討
Sep. 1981, [Reviewed]

気管支作動性物質の作用に及ぼすBD 40A (Formoterol) の効果について　　　　　　　　　　　　　　　
Aug. 1981, [Reviewed]

びまん性陰影を呈する疾患における経気管支肺生検(TBLB)の意義について　　　　　　　　　　　　　　　
Jul. 1981

癌性心膜炎の tetracycline 療法
Jul. 1981

慢性気管支炎患者に対するトラネキサム酸の効果 気管支ファイバースコープを用いた臨床的研究　　　　　　　　　　　　　　　
Jun. 1981

喀毛をきたした縦隔奇形腫の1例
Jun. 1981

クリプトコッカス症を合併したCushing症候群の１例　　　　　　　　　　　　　　　
Apr. 1981

組織培養に成功したアミラーゼ産生肺がんの1剖検例
Mar. 1981

Angiotensin converting enzyme activity in patients with spontaneous pneumothorax and in rabbits with artificial pneumothorax.　　　　　　　　　　　　　　　
Kitamura, S; Ishihara, Y; Sugiyama, Y; Izumi, T; Hayashi, R; Hsu, L.-H; Kosaka, K
Proceedings of 7th Asia pacific congress on disease of the chest. (Nandi, P. L., Lam, W. K., eds.), 1981

Transbronchial biopsy through the flexible fiberoptic bronchoscope: a review of 225 cases　　　　　　　　　　　　　　　
Izumi, T; Sugiyama, Y; Hsu, L.-H; Hayashi, R; Kitamura, S; Kosaka, K; Shiraki, R; Kinoshita, M; Wagai, F
Proceedings of 7th Asia pacific congress on disease of the chest. (Nandi, P. L., Lam, W. K., eds.), 1981

気管支筋作動薬の作用に及ぼすdisodium cromoglycate（DSCG）の効果について
Jan. 1981, [Reviewed]

Intrapleural doxycycline for control of malignant pleural effusion
S KITAMURA; Y SUGIYAMA; T IZUMI; R HAYASHI; K KOSAKA
CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL, 1981, [Reviewed]

Treatment of carcinomatous pericarditis with doxycycline - intra-pericardial doxycycline for control of malignant pericardial-effusion
S KITAMURA; F WAGAI; T IZUMI; Y SUGIYAMA; K KOSAKA
CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL, 1981, [Reviewed]

ウサギ肺におけるdirect β-adrenergicreceptorassay -isoproterenol,solbutamol,prednisolone投与の影響について　　　　　　　　　　　　　　　
Dec. 1980, [Reviewed]

家兎肺および気管筋における15-hydroxy prostaglandin dehydrogenase (15-OHPGDH)阻害剤の作用について　　　　　　　　　　　　　　　
Nov. 1980

Comparison of the Effect of PGD2 and PGF2a on Guinea Pig Tracheal Strips　　　　　　　　　　　　　　　
Ishihara Yoko; Sugiyama Yukihiko; Hsu Lung-Hung; Izumi Takashi; Hayashi Ryujiro; Kitamura Satoshi
Sep. 1980, [Reviewed]

Effect of Ipratropium Bromide (Sch 1000) on the Action of Various Bronchoactive Agents　　　　　　　　　　　　　　　
Yoko Ishihara; Takashi Izumi; Ryujiro Hayashi; Lung-Hung Hsu; Yukihiko Sugiyama; Satoshi Kitamura
Aug. 1980, [Reviewed]

Effect of 15-Hydroxy Prostaglandin Dehydrogenase Inhibitor on the Degradation of Prostaglandins through Guinea Pig Lung Lobes and in Tracheal Strips　　　　　　　　　　　　　　　
Yoko Ishihara; Ryujiro Hayashi; Lung-Hung Hsu; Takashi Izumi; Yukihiko Sugiyama; Satoshi Kitamura
The Japanese journal of thoracic diseases, Aug. 1980, [Reviewed]

内視鏡的に経過を観察しえた気管・気管支結核の一症例　　　　　　　　　　　　　　　
Jul. 1980

Effect of Prostaglandins on Anaphylactic Release of Histamine and Slow Reacting Substance of Anaphylaxis (SR-A) from Guinea pig Lung Tissues　　　　　　　　　　　　　　　
Satoshi Kitamura; Yoko Ishihara; Takashi Izumi; Yukihiko Sugiyama; Ryujiro Hayashi; Lung-Hung Hsu
Jul. 1980, [Reviewed]

気管支収縮剤の作用におよぼすβ受容体遮断剤の効果について　　　　　　　　　　　　　　　
Jul. 1980, [Reviewed]

プロスタグランディンの代謝と肺疾患　　　　　　　　　　　　　　　
Jun. 1980

気管支喘息の診断基準・病型分類・重症度　　　　　　　　　　　　　　　
Jun. 1980

Effect of Aspirin and Indomethacin on the Immunological Release of Histamine and Slow Reacting Substance of Anaphylaxis (SRS-A) from Guinea-pig Lung Tissues　　　　　　　　　　　　　　　
S. Kitamura; Y. Ishihara; T. Izumi; K. Kosaka
THE JAPANESE JOURNAL OF THORACIC DISEASES, May 1980, [Reviewed]

A case of Gilbert's syndrome- A study of bilirubin mono- and di-glucuronide in the liver and bile
IZUMI Takashi; INAGAKI Tooru; KATAMOTO Tetsuro; OKANO Kenichi; HIRANO Masanori; TERANO Akira; MATSUMOTO Kazunori; MOTOKI Tatsuya; KAMII Kazuo; MURAO Satoru; KAMISAKA Kazuaki
Kanzo, Apr. 1980, [Reviewed]

慢性気管支炎, 気管支拡張症患者におけるレスプレンの使用経験　　　　　　　　　　　　　　　
Mar. 1980

慢性閉塞性肺疾患とt-AMCHA　　　　　　　　　　　　　　　
1980

気管支喘息のα受容体遮断剤療法-その基礎と臨床-　　　　　　　　　　　　　　　
Dec. 1979

肺・気管支系炎症とt-AMCHA　　　　　　　　　　　　　　　
1979

多機能酵素によるロイコトリエンの代謝　　　　　　　　　　　　　　　
Jun. 2024, [Invited]
Lead

骨芽細胞様細胞株MC3T3-E1におけるPGE2分泌を介したメカノストレス伝播機構の解析　　　　　　　　　　　　　　　
May 2023

テロメア短縮系を用いた効率的な複製老化系の樹立および分岐鎖アミノ酸代謝の細胞老化における役割の解析
2023

Investigation of Thinning Effect on Sensitivity of Silicon Nanowire Biosensor for Ultra-Sensitive and Specific Biomolecule Detection　　　　　　　　　　　　　　　
Hui Zhang; Yawei Qiu; Fumiya Osawa; Meiko Itabashi; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
MNC 2022, 35th International Microprocesses and Nanotechnology Conference, Nov. 2022

マウス由来GDE7は環状ホスファチジン酸を産生する酵素活性を有する
Jun. 2022
Last, Corresponding

Murine GDE4 and GDE7 produce lysophosphatidic acid and/or cyclic phosphatidic acid　　　　　　　　　　　　　　　
Binderiya Tserendabga; 大嶋 紀安; 藤田 千明; 湯澤 厚二; 大嶋 麻理; 矢中 規之; 南嶋 洋司; 和泉 孝志
日本生化学会大会プログラム・講演要旨集, Nov. 2021
Last, Corresponding, (公社)日本生化学会

Modulation of Cellular Senescence by Branched-Chain Amino Acid Metabolism
2021

MC3T3-E1における,脂質オートクライン刺激とエストラジオール刺激のクロストーク機構の解析
2020

キャピラリー電気泳動を用いる唾液中の陰及び陽イオンの同時分析と生理活性物質との相関　　　　　　　　　　　　　　　
Aug. 2019

LipidBankデータベースの歴史と現状
Jun. 2019
Lead, 日本脂質生化学会

Fabrication of High-Sensitivity Silicon Nanowire Biosensor by Electron Beam Lithography and Detection of Antigen-Antibody Specific Binding at Attomolar Concentration　　　　　　　　　　　　　　　
Mar. 2019

シリコンナノワイヤ細線化によるバイオセンサ検出感度の評価
2019

骨芽細胞様細胞MC3T3-E1の細胞内外の脂質メディエーターがアクチン細胞骨格変化を介して骨形成を制御する
2019

Analysis of inflammatory lipid mediators induced matrix mineralization regulation mechanism of MC3T3-E1 osteoblast-like cells via actin cytoskeleton reorganization　　　　　　　　　　　　　　　
Hiraku Suzuki; Noriyasu Ohsima; Akimitsu Konishi; Kazuaki TateiSeiichi Sato; Takashi Izumi
第41回日本分子生物学会年会 講演要旨, Nov. 2018
Last, Corresponding

刺激依存性・海馬歯状回特異的新規ホスホリパーゼA2 (KIDSScPLA2)　　　　　　　　　　　　　　　
Jul. 2018

一般家庭における重金属曝露メカニズムの解明 調査プロトコルについて　　　　　　　　　　　　　　　
西浜 柚季子; 龍田 希; 岩井 美幸; 磯部 友彦; 藤原 幾磨; 有馬 隆博; 中山 祥嗣; 和泉 孝志; 仲井 邦彦
日本衛生学雑誌, Mar. 2018
(一社)日本衛生学会

A membrane receptor, G2A induces epithelial-to-mesenchymal transition-like phenomenon in human astrocytoma cell lines
Kishimoto Koji; Haraguchi Takashi; Shimizu Kenji; Izumi Takashi
CANCER SCIENCE, Jan. 2018, [Reviewed]
Last, Corresponding

脂質データベースLipidBankのWiki版構築とその特徴　　　　　　　　　　　　　　　
Dec. 2017
Last, 生命科学系学会合同年次大会運営事務局

膜貫通型リゾホスホリパーゼD、GDE4過剰発現細胞における脂質の網羅的解析　　　　　　　　　　　　　　　
Dec. 2017
Last, 生命科学系学会合同年次大会運営事務局

骨芽細胞様細胞MC3T3-E1における分化段階に応じた機械的刺激による骨形成制御応答の解析　　　　　　　　　　　　　　　
鈴木 啓; 大嶋 紀安; 小西 昭充; 立井 一明; 佐藤 精一; 和泉 孝志
生命科学系学会合同年次大会, Dec. 2017
Last, 生命科学系学会合同年次大会運営事務局

ゲノム編集技術を用いた蛍光蛋白ラベル法による内在性p53蛋白動態の1細胞解析　　　　　　　　　　　　　　　
Dec. 2017
Last, 生命科学系学会合同年次大会運営事務局

スフィンゴシン1リン酸の新規担体分子の探索　　　　　　　　　　　　　　　
Dec. 2017
Last, 生命科学系学会合同年次大会運営事務局

膜受容体G2Aはアストロサイトーマ細胞株にグリア間葉移行を誘導する　　　　　　　　　　　　　　　
Sep. 2017
Last, 日本癌学会

電子線リソグラフを用いた超高感度Siナノワイヤバイオセンサの創製　　　　　　　　　　　　　　　
2017
Last

群馬大学全体を統合した研究支援体制の強化 群馬大学の研究推進体制と未来先端研究機構について　　　　　　　　　　　　　　　
Aug. 2016
Lead, 北関東医学会

私はなぜ研究医を目指したか 文部科学省GPプログラムや他の取組みの検証 群馬大学「卒前・卒後一貫MD-PhDコース」を経験して
Jul. 2016
(一社)日本医学教育学会

可溶性2-アラキドノイルグリセロール産生酵素、DDHD2の酵素学的解析　　　　　　　　　　　　　　　
May 2016
Last

Cell cycle-dependent regulation of non-homologous end joining at unprotected telomeres　　　　　　　　　　　　　　　
Kadiombo Anastasie T.; Konishi Akimitsu; Izumi Takahi
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Dec. 2015
Last, (公社)日本生化学会

脂質データベースLipidBank Wiki版におけるスフィンゴ糖脂質の分類　　　　　　　　　　　　　　　
Dec. 2015
Last, (公社)日本生化学会

新規リゾホスファチジン酸産生酵素、GDE4・GDE7の酵素学的解析　　　　　　　　　　　　　　　
May 2015
Last

新規酵素GDE4およびGDE7は,LPCおよびlysoPAFを分解し,LPAおよびalkyl‐LPAの産生に関与する
24 Jan. 2015

Purification and characterization of a soluble diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
Mari Araki; Chizu Aso; Noriyasu Ohshima; Kazuaki Tatei; Tohko Hirano; Hiroyuki Sugimoto; Mikiko Kishi; Koji Kishimoto; Hideru Obinata; Takashi Izumi
14th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases, 2015
Last, Corresponding

GDE4 and GDE7 Produce Lysophosphatidic Acid by Lysophospholipase D Activity　　　　　　　　　　　　　　　
Mari Araki; Chizu Aso; Noriyasu Ohshima; Kazuaki Tatei; Tohko Hirano; Hiroyuki Sugimoto; Mikiko Kishi; Koji Kishimoto; Takashi Izumi
6th international conference on Phospholipase A2 and Lipid Mediators (PLM2015) Abstract, 2015
Last

Identification and functional analyses of a novel diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
Chiaki Isaji; Takahiro Kudo; Noriyasu Ohshima; Yosuke Yamashita; Mari Araki; Ayako Honda; Tomomi Nagano; Norihisa Kato; Akiyoshi Konishi; Koji Kishimoto; Kazuaki Tatei; Noriyuki Yanaka; Takashi Izumi
6th international conference on Phospholipase A2 and Lipid Mediators (PLM2015) Abstract, 2015

ラット脳ジアシルグリセロールリパーゼの精製と機能解析　　　　　　　　　　　　　　　
Aug. 2014
Last

群馬大学におけるAdvanced OSCEの試み 医療コミュニケーション能力の評価を目的とした新規課題の導入　　　　　　　　　　　　　　　
Jul. 2014
(一社)日本医学教育学会

LipidBankにおける糖脂質分類：植物編　　　　　　　　　　　　　　　
Jun. 2014
Last

Gタンパク質共役型受容体,G2Aが制御するがん幹細胞性と上皮間葉転換
2014

9-HODE-activated GPCR, G2A induces epithelial-to-mesenchymal transition of human astrocytoma cells prior to generation of stem cell-like properties.　　　　　　　　　　　　　　　
Kishimoto K; Haraguchi T; Shimizu K; Yamaguchi A; Yoshihara T; Ohshima N; Tatei K; Tobita S; Tsushima Y; Izumi T
Society of Neuroscience 2013 Abstract (WEB), Nov. 2013
Last

Gタンパク質共役型受容体、G2Aはヒトアストロサイトーマ細胞株のがん幹細胞性を制御する
Sep. 2013
Last, (公社)日本生化学会

新規ラット脳ジアシルグリセロールリパーゼの同定
Sep. 2013
Last, Corresponding, (公社)日本生化学会

糖尿病母ラットから産まれた仔の心筋細胞におけるインシュリンシグナル異常を改善するEPAの効果　　　　　　　　　　　　　　　
Sep. 2013
(公社)日本生化学会

LipidBankにおけるArchaea脂質のデータベース　　　　　　　　　　　　　　　
Sep. 2013
Last

基礎学力の向上を目指す教養教育科目「学びのリテラシー」の実践　　　　　　　　　　　　　　　
Jul. 2013
Last, (一社)日本医学教育学会

新規脳ジアシルグリセロールリパーゼの同定
28 May 2013
Last, Corresponding

新しいLipidBankにおける糖脂質分類
28 May 2013
Last

新規酵素GDE7はオートタキシン活性を有し，LPAおよびalkyl-LPAの産生に関与する　　　　　　　　　　　　　　　
Mar. 2013

卒業後を振り返って　　　　　　　　　　　　　　　
10 Jan. 2013

Subcellular distribution and binding specificity of phospholipid-binding proteins
Xi Xin; Kazuaki Tatei; Hideaki Ehara; Yumiko Kihara; Takashi Izumi
JCBL, 2013
Last, Corresponding

アーキア脂質のデータベース
2013
Last, Corresponding

血管平滑筋細胞に対するニコチンの分化・脱分化作用に関する解析
2013

ヒトアストロサイトーマU251細胞の幹細胞性と上皮間葉転換制御に関わるG2Aの役割　　　　　　　　　　　　　　　
Dec. 2012
Last, Corresponding, (公社)日本生化学会

抗ロイコトリエンC4モノクローナル抗体とその単鎖抗体によるロイコトリエン中和作用
Dec. 2012
(公社)日本生化学会

Oxidized Free Fatty Acids-Activated GPCR, G2A Regulates Stemness and Malignant Behavior of An Astrocytoma Cell Line.　　　　　　　　　　　　　　　
Koji Kishimoto; Takashi Haraguchi; Kenji Shimizu; Aiko Yamaguchi; Toshitada Yoshihara; Noriyasu Ohshima; Kazuaki Tatei; Seiji Tobita; Yoshito Tsushima; Takashi Izumi
Society for Neuroscience 2012 Abstract (WEB), Oct. 2012
Last, Corresponding

G2Aはアストロサイトーマ細胞の幹細胞性と悪性度を制御する(G2A Regulates Stemness and malignant behavior of Astrocytoma Cells)　　　　　　　　　　　　　　　
Aug. 2012
日本癌学会

アンケート調査からみた生涯教育への臨床医のニーズ 群馬大学医学教育センターリカレント教育部門の活動　　　　　　　　　　　　　　　
Jul. 2012
Corresponding, (一社)日本医学教育学会

酸化遊離脂肪酸をリガンドとするGタンパク質共役型受容体、G2Aはアストロサイトーマ細胞株のがん幹細胞性を制御し、悪性度を規定する　　　　　　　　　　　　　　　
Jun. 2012

表面プラズモン共鳴現象を利用した効率的なGPCRリガンドスクリーニング方法
Jun. 2012

ロイコトリエン作用に対する抗ロイコトリエンC4単鎖抗体の中和効果
28 May 2012
日本脂質生化学会

Gタンパク質共役型受容体、G2Aを介したがん幹細胞性制御
28 May 2012
Last

医学生の学力低下雑感　　　　　　　　　　　　　　　
2012
Lead

抗ロイコトリエンC4モノクローナル抗体とその単鎖抗体によるロイコトリエン中和作用
Sep. 2011
(公社)日本生化学会

G2Aは神経膠腫細胞株U251に栄養飢餓及び低酸素刺激に対する抵抗性を賦与する
Sep. 2011
Last, Corresponding, (公社)日本生化学会

Expression of a single-chain antibody that neutralizes the effect of cysteinyl leukotrienes
Yoshitaka Takahashi; Yuki Kawakami; Shiori Hirano; Mai Kinoshita; Akemi Otsuki; Toshiko Suzuki-Yamamoto; Yuko Kurahashi; Hiromi Daiyasu; Hiroyuki Toh; Koji Kishimoto; Takashi Izumi; Shozo Yamamoto
CHEMISTRY AND PHYSICS OF LIPIDS, Aug. 2011, [Reviewed]

哺乳動物のGDE群とマウスGDE5の非酵素的機構による骨格筋分化制御
28 Apr. 2011
Last

抗ロイコトリエンC₄モノクローナル抗体とその単鎖抗体によるロイコトリエン中和作用
2011

ロイコトリエンに対する単鎖抗体を利用した抗体治療薬開発に向けた基礎研究
2011

シスタチオニンγ-リアーゼ欠損マウスにおける食餌性急性致死性ミオパチー(Diet-induced acute lethal myopathy in mice lacking cystathionine gamma-lyase)
Dec. 2010
(公社)日本生化学会

マウス由来グリセロホスホジエステルホスホジエステラーゼ1(GDE1)の基質特異性と組織分布の解析　　　　　　　　　　　　　　　
Dec. 2010
Last, (公社)日本生化学会

pColdベクターを用いた5-リポキシゲナーゼ(5-LO)の大量発現と精製
Dec. 2010
Last, (公社)日本生化学会

酪酸ナトリウムでラット肝臓幹様細胞のAnnexin A3の発現が抑制される(Annexin A3 mRNA repression by sodium butyrate in rat hepatic stem-like cells)　　　　　　　　　　　　　　　
Dec. 2010
(公社)日本生化学会

細胞質ホスホリパーゼA2αはマウスにおける脳虚血後の早期のシクロオキシゲナーゼ2発現、酸化ストレスおよびMAPキナーゼリン酸化を増強する(Cytosolic phospholipase A2 alpha amplifies early cyclooxygenase-2 expression, oxidative stress and MAP kinase phosphorylation after cerebral ischemia in mice)　　　　　　　　　　　　　　　
Dec. 2010
Last, (公社)日本生化学会

酸化遊離脂肪酸をリガンドとするG2Aの機能とサブタイプの解析
25 May 2010
Last

アシルプロテインチオエステラーゼ1/リゾホスホリパーゼ1の活性と細胞内局在
25 May 2010
Last

Novel function of 9(S)-hydroxyoctadecadienoic acid on proliferation of T cells　　　　　　　　　　　　　　　
2010
Last, Corresponding

ほ乳類グリセロホスホジエステルホスホジエステラーゼの酵素学的解析　　　　　　　　　　　　　　　
Nov. 2009
Last

CSE欠損マウスにおける高メチオニン餌投与による急性肝炎
Sep. 2009
(公社)日本生化学会

酪酸ナトリウムに対して可逆的に反応するラット肝臓幹様細胞のプロテオーム解析　　　　　　　　　　　　　　　
Sep. 2009
(公社)日本生化学会

表面プラズモン共鳴現象による生細胞におけるRTKシグナルの検出　　　　　　　　　　　　　　　
陳 可欣; 大日方 英; 岸 美紀子; 立井 一明; 和泉 孝志
日本生化学会大会プログラム・講演要旨集, Sep. 2009
(公社)日本生化学会

表面プラズモン共鳴現象を用いた生細胞におけるGPCRシグナルの検出　　　　　　　　　　　　　　　
Jul. 2009
Last

ショウジョウバエ培養細胞Schneider株においてセラミドは筋分化とアポトーシスを誘導する
Jul. 2009
Last, Corresponding

新規因子GDE5の筋芽細胞分化における役割、および骨格筋特異的GDE5過剰発現マウスの形質の解析　　　　　　　　　　　　　　　
Mar. 2009

Characterization of mouse glycerophosphodiester phosphodiesterase 1 (GDE1)　　　　　　　　　　　　　　　
Ohshima N; Kaneko M; Takahashi N; Takio K; Shiro Y; Tatei T; Izumi T
4th International Conference on Phospholipase A2 and Lipid Mediators (PLM2009) Abstract, 2009
Last

Study of butyrate signal transduction pathways in rat hepatic stem-like cells　　　　　　　　　　　　　　　
Saheki T; Mukai Y; Saito K; Tajima E; Katakura K; Nishina A; Kishi M; Izumi T; Sugiyama T; Kojima I
Animal Cell Technol.: Basic & Applied Aspects, Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), 2009

PAF receptor
IZUMI Takashi
Folia pharmacologica Japonica, 01 Dec. 2008
Lead

M-CSF刺激された破骨細胞前駆細胞において転写因子Ets2はリン脂質合成酵素CTαの転写を促進する　　　　　　　　　　　　　　　
Aug. 2008
(公社)日本整形外科学会

脂質メディエーターの新規代謝経路と生理機能 酸化遊離脂肪酸をリガンドとするG2Aの生理機能
Jun. 2008
Lead, 日本脂質生化学会

リゾホスホリパーゼ1の脱パルミトイル化活性の解析　　　　　　　　　　　　　　　
May 2008
Last

Gタンパク質共役型受容体G2Aの新規スプライシングバリアントの同定と機能解析　　　　　　　　　　　　　　　
May 2008
Last

マウス由来グリセロホスホジエステルホスホジエステラーゼ１(GDE1)の酵素学的解析　　　　　　　　　　　　　　　
2008
Last

脱パルミトイル化酵素としてのリゾホスホリパーゼ1の解析　　　　　　　　　　　　　　　
平野 瞳子; バルリアナ・メリサ・インタン; 岸 美紀子; 杉本 博之; 村田 千恵; 田口 良; 立井 一明; 和泉 孝志
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, Nov. 2007
(公社)日本生化学会

ショウジョウバエ培養細胞における紫外線誘発アポトーシスの解析
河村 英将; 立井 一明; 野中 哲生; 大日方 英; 服部 友保; 小川 愛; 風間 秀子; 浜田 信行; 舟山 知夫; 坂下 哲哉; 小林 泰彦; 和泉 孝志; 中野 隆史
日本放射線影響学会大会講演要旨集, 01 Nov. 2007
Journal of Radiation Research 編集委員会

転写因子Ets2は核移行シグナル(NLS)と核外移行シグナル(NES)を有し、リン脂質合成酵素CTαの転写を促進する　　　　　　　　　　　　　　　
岡邨 興一; 高岸 憲二; 山下 智子; 和泉 孝志; 杉本 博之
日本整形外科学会雑誌, Aug. 2007
(公社)日本整形外科学会

表皮角化細胞におけるGタンパク質共役型受容体G2Aの生理作用
05 Jun. 2007
Last

Ets2の核内(NLS)および核外移行シグナル(NES)の同定およびRAW293細胞を用いたM-CSF刺激によるCTP: phosphocholine cytidylyltransferaseα(CTα)転写促進におけるEts2の重要性
2007

Analysis of radiation-induced apoptosis in early Drosophila embryos　　　　　　　　　　　　　　　
Tatei, K; Tamaki, T; Kawamura, H; Hamada, N; Sakashita, T; Funayama, T; Wada, S; Kakizaki, T; Nonaka, T; Obinata, H; Hattori, T; Ogawa, A; Kazama, H; Nakano, T; Kobayashi, Y; Izumi, T
JAEA-Review 2006-042 TAKASAKI Annual Report 2005, 2007
Last, Corresponding

ショウジョウバエの初期胚に対する重粒子線照射の影響
田巻倫明; 野中哲生; 河村英将; 風間秀子; 中野隆史; 立井一明; 大日方英; 服部友保; 和泉孝志; 浜田信行; 和田成一; 小林泰彦; 舟山知夫; 坂下哲哉; 柿崎竹彦
Kitakanto Med J, 01 Aug. 2006
北関東医学会

質量分析法を用いたリゾホスホリパーゼによるパルミトイル化ペプチドの検出
08 Jun. 2006
Last

Gタンパク質共役型受容体G2Aのリガンド同定とシグナル解析　　　　　　　　　　　　　　　
大日方 英; 服部 友保; 立井 一明; 和泉 孝志
脂質生化学研究, May 2006
Last, 日本脂質生化学会

酸化遊離脂肪酸の受容体G2Aのヒト表皮角化細胞における生理的役割　　　　　　　　　　　　　　　
Apr. 2006
Last, Corresponding, (一社)日本研究皮膚科学会

Bioluminescence Resonance Energy Transfer によるロイコトリエンB4 受容体二量体の検出
02 Jun. 2005
Last

多機能酵素としての低分子型リゾホスホリパーゼとその質量分析への応用　　　　　　　　　　　　　　　
May 2005
Lead

カンチレバー型フェムトグラムバイオセンサを用いた抗体抗原反応測定
29 Mar. 2005

カンチレバー共振型ピコグラムセンサー
01 Sep. 2004

CTP : phosphocholine cytidylyltransfease α 転写のEts-1およびSp1による協調的促進と Net による抑制
18 Jun. 2004
Last

Detection of the Leukotriene B-4 receptor dimerization/oligomerization in living cells by Bioluminescence Resonance Energy Transfer (BRET)
Mikiko Kishi; Takashi Izumi
CELL STRUCTURE AND FUNCTION, May 2004
Last, Corresponding

Intracellular localization and activity regulation of 5-lipoxygenase
T Izumi; H Hanaka; M Suzuki; T Shimizu
JOURNAL OF PHARMACOLOGICAL SCIENCES, 2004
Lead

LTA4水解酵素の細胞内局在とそのメカニズムについて　　　　　　　　　　　　　　　
May 2003

5-oxo-ETE受容体の活性化が引き起こすケモタキシスと細胞内セカンドメッセンジャー濃度変化の測定　　　　　　　　　　　　　　　
May 2003
日本脂質生化学会

刺激依存性・海馬歯状回特異的新規ホスホリバーゼA2 アイソフォーム　　　　　　　　　　　　　　　
Dec. 2002

「おさかな天国」頭と体によい魚の脂　　　　　　　　　　　　　　　
10 Oct. 2002, [Invited]
Lead

生理活性脂質の基礎と臨床　　　　　　　　　　　　　　　
The Kitakanto Medical Journal, Sep. 2002
Lead, 北関東医学会

医学と工学のクロストーク2002 生理活性脂質LPAの分析法に関する研究　　　　　　　　　　　　　　　
The Kitakanto Medical Journal, Sep. 2002
Last, Corresponding, 北関東医学会

臨床応用をめざした血中生理活性脂質の簡便な定量法の確立　　　　　　　　　　　　　　　
Sep. 2002
Lead, (公財)先進医薬研究振興財団

ロイコトリエンB4受容体-1のグルココルチコイドによる発現上昇　　　　　　　　　　　　　　　
Aug. 2002
Last, (公社)日本生化学会

キイロショウジョウバエのホスホリパーゼD遺伝子の単離と解析
14 Jun. 2002
Last

私の脂質研究と今後　　　　　　　　　　　　　　　
2002
Lead

Leukotriene B-4 and its pathological roles
T Izumi; T Yokomizo; T Shimizu
JAPANESE JOURNAL OF PHARMACOLOGY, 2002
Lead

プロスタグランジン及びロイコトリエン生合成酵素の分子構造と機能 5-リポキシゲナーゼの細胞内局在と移行　　　　　　　　　　　　　　　
Aug. 2001
Lead, (公社)日本生化学会

アラキドン酸5-リポキシゲナーゼの核外移行に関する解析　　　　　　　　　　　　　　　
Aug. 2001
Last, Corresponding, (公社)日本生化学会

CTP phosphocholine cytidylyltransferase α (CCTα)転写のTranscriptional enhancer factor-4(TEF-4)による促進とForskolinとDibutyryl cAMPによる抑制　　　　　　　　　　　　　　　
Aug. 2001
(公社)日本生化学会

刺激依存性・海馬歯状回特異的新規ホスホリパーゼA2(KIDScPLA2)
岸本 幸治; 白川 純; 魚住 尚紀; 和泉 孝志; 黒柳 秀人; 鈴木 陽一; 白澤 卓二; 中館 和彦; 渡辺 恭良; 清水 孝雄
生化学, Aug. 2001
(公社)日本生化学会

刺激依存性・海馬歯状回特異的新規ホスホリパーゼA2(KIDScPLA2)の酵素学的性質の検討　　　　　　　　　　　　　　　
Aug. 2001
(公社)日本生化学会

マウスCysLT1のクローニングと発現　　　　　　　　　　　　　　　
Aug. 2001
(公社)日本生化学会

ロイコトリエンD4によるミクログリアの活性制御　　　　　　　　　　　　　　　
Aug. 2001
(公社)日本生化学会

マウスシステイニルロイコトリエン2受容体(CysLT2)の解析　　　　　　　　　　　　　　　
Aug. 2001
(公社)日本生化学会

マクロファージにおけるPAF受容体依存的なPAF分解　　　　　　　　　　　　　　　
大嶋 紀安; 石井 聡; 和泉 孝志; 清水 孝雄
生化学, Aug. 2001
(公社)日本生化学会

アラキドン酸代謝研究の進歩　　　　　　　　　　　　　　　
Jul. 2001

5‐リポキシゲナーゼの細胞内局在と移行
Jul. 2001
Last, 日本炎症・再生医学会

マウス腹腔マクロファージにおける受容体依存的なPAFの分解
10 May 2001
日本脂質生化学会

ロイコトリエンB4受容体のグルココルチコイドによる発現制御
10 May 2001
日本脂質生化学会

PP117 肺癌組織におけるTEF-4mRNA及びCCTmRNA発現量の検討
10 Mar. 2001
Last

炎症・アレルギー性疾患の発症機構の解明と医療への応用 (ヒューマンサイエンス振興財団S)　　　　　　　　　　　　　　　
2001

Receptor-Dependent Metabolism of PAF by Macrophages　　　　　　　　　　　　　　　
Ohshima N; Ishii S; Izumi T; Shimizu T
Seventh International Congress on Platelet-Activating Factor and Lipid Mediators Abstract, 2001

Elucidation of the crisis mechanism of inflammation and allergosis and application to the medical treatment ( human science promotion foundation S ).
2001

ゲノムと日本語　　　　　　　　　　　　　　　
Sep. 2000
Lead

脂質メディエーター研究の新展開 ロイコトリエンの産生制御と受容体　　　　　　　　　　　　　　　
Aug. 2000
Lead, (一社)日本細胞生物学会

第二のロイコトリエンB4受容体BLT2のクローニングと解析　　　　　　　　　　　　　　　
Aug. 2000
(公社)日本生化学会

脂質メディエーター研究の最前線 ロイコトリエン受容体　　　　　　　　　　　　　　　
Aug. 2000
Lead, (公社)日本生化学会

脂質メディエーター研究の最前線 刺激依存性・海馬歯状回特異的新規ホスホリパーゼA2アイソフォーム　　　　　　　　　　　　　　　
Aug. 2000
(公社)日本生化学会

ロイコトリエンD4受容体を介したミクログリアの活性化　　　　　　　　　　　　　　　
Aug. 2000
(公社)日本生化学会

ヒトロイコトリエンB₄受容体遺伝子の転写制御
10 Jul. 2000

アラキドン酸カスケード最近の進歩　　　　　　　　　　　　　　　
Jul. 2000

脂質メディエーターと生体制御-ロイコトリエン受容体を中心に　　　　　　　　　　　　　　　
May 2000
Last, 日本脂質生化学会

マウスのロイコトリエンD4受容体のクローニングと発現　　　　　　　　　　　　　　　
May 2000
日本脂質生化学会

広範肝切除後肝障害 特に肝におけるサイトカイン産生・PAF receptor発現動態ならびにPGI2の影響を中心として
Mar. 2000
(一社)日本外科学会

広範肝切除後の外科的侵襲 PAF recepter発現動態及び炎症性サイトカイン・Prostaglandin E2の産生を中心として
Mar. 2000
Last, (一社)日本外科学会

細胞内情報伝達 アラキドン酸カスケードの新展開　　　　　　　　　　　　　　　
Dec. 1999
Lead, 日本医学会

【肝障害の成立と類洞壁細胞,サイトカインの関わり】 広範肝切除後肝障害におけるPAF receptor発現動態 発現局在とProstaglandin I2の影響について　　　　　　　　　　　　　　　
水野 修吾; 川原田 嘉文; 長尾 美昌; 高木 馨子; 野田 直哉; 近藤 昭信; 和泉 孝志; 清水 孝雄
May 1999
(一社)日本肝臓学会

EGFPとの融合タンパク質を用いた5-リポキシゲナーゼの細胞内局在と膜移行の解析　　　　　　　　　　　　　　　
May 1999
日本脂質生化学会

モルモットロイコトリエンB4受容体cDNAのクローニング及び機能解析　　　　　　　　　　　　　　　
May 1999
日本脂質生化学会

ブタ白血球ロイコトリエンB4受容体の3量体G蛋白質との分離と再構成　　　　　　　　　　　　　　　
Apr. 1999
(一社)日本内分泌学会

O-71 広範肝切除後肝障害 : 特にPAF recepter発現動態とProstaglandin I2の影響について
10 Feb. 1999

炎症・アレルギー性疾患の発症機構の解明と医療への応用 (ヒューマンサイエンス振興財団S)
1999

プロスタノイドとレセプターの新展開 ロイコトリエンB4受容体の構造と細胞内情報伝達　　　　　　　　　　　　　　　
Aug. 1998
(公社)日本生化学会

Aminopeptidase(AP)-Bのepoxide hydrolase活性について　　　　　　　　　　　　　　　
Aug. 1998
(公社)日本生化学会

EGFPとの融合タンパク質を用いた5-リポキシゲナーゼの膜移行の解析　　　　　　　　　　　　　　　
Aug. 1998
(公社)日本生化学会

Leukotriene A4 Hydrolaseの細胞内移行　　　　　　　　　　　　　　　
Aug. 1998
(公社)日本生化学会

モルモットLTB4受容体cDNAのクローニング及びその解析　　　　　　　　　　　　　　　
増田 一之; 横溝 岳彦; 和泉 孝志; 清水 孝雄
生化学, Aug. 1998
(公社)日本生化学会

モルモットのロイコトリエンB4 12水酸基脱水素酵素の性質　　　　　　　　　　　　　　　
山本 寿子; 横溝 岳彦; 和泉 孝志; 清水 孝雄
生化学, Aug. 1998
(公社)日本生化学会

ロイコトリエンとアレルギー疾患 ロイコトリエン受容体
Mar. 1998

1 ロイコトリエン受容体(シンポジウム5 ロイコトリエンとアレルギー疾患 : 気管支喘息とアレルギー性鼻炎を中心に)
1998
Lead

Elucidation of the pathogeny mechanism of the allergy inflammation control substance and development of the strategy of drug exploration. ( Human science promotion foundation S ).
1998

Primary structures and NADP(+) binding domain of leukotriene B-4 12-hydroxydehydrogenases.
T Yokomizo; T Yamamoto; T Izumi; T Shimizu
FASEB JOURNAL, Jul. 1997

気管支喘息の受容体発現とその調節を目的とする治療構成 PAF受容体 遺伝子から臨床まで　　　　　　　　　　　　　　　
Mar. 1997
Lead, (一社)日本呼吸器学会

Increased expression of PAF acceptor in eosinophil of atopic bronchial asthma patients and its regulation mechanism.
Mar. 1997

PAF と気管支喘息　　　　　　　　　　　　　　　
1997
Lead

気管支喘息の受容体発現とその調節を目的とする治療構成
1997

Elucidation of the crisis mechanism of allergy inflammation control substance, search for its drug, and development of its strategy. ( Human Science Promotion Foundation S ).
1997

Leukotriene B_4 12-hydroxydehydrogenaseの補酵素結合部位の同定
Aug. 1996

アレルギー性気管支喘息における好酸球PAF受容体の発現亢進
01 Aug. 1996

マクロファージに於けるロイコトリエンD4によるアラキドン酸の放出
01 Aug. 1996

発生工学によるPAF受容体の機能の解析
01 Aug. 1996

アラキドン酸の長い旅　　　　　　　　　　　　　　　
1996
Lead

Expression of platelet-activating factor receptor in EoL-1 cells during differentiation.
Aug. 1995
Lead

Interleukin‐5による好酸球血小板活性化因子(PAF)受容体の発現誘導
Mar. 1995

Research on the basic sciences to invent drugs. Research on the search for lead compounds based on the elucidation of biofunctions and outbreaking mechanism of diseases. Basic researches on the inflammation mediator synthesis inhibitors, receptor antago
1995

cDNA cloning and expression of LTB4 12-hydroxydehydrogenase.
1995

Research on basic pharmaceutical science. Research on the search of lead chemical compounds based on elucidation of biofunctions and disease crisis mechanism. Basic researches on inflammation mediator synthesis inhibitor and receptor antagonist. ( Huma
1995

Leukotriene B₄(LTB₄)の新しい不活化経路 (II) 代謝物の構造決定
Jun. 1994

Leukotriene B₄(LTB₄)の新しい不活化経路 (I) LTB₄ 12‐Hydroxydehydrogenaseの精製と性質
Jun. 1994

Eicosanoid, PAF receptor and signal transfer.
Apr. 1994
Lead

血小板活性化因子による生態制御 PAF受容体のクローニングと情報伝達　　　　　　　　　　　　　　　
Oct. 1993
Lead, (一社)日本血液学会-東京事務局

EoL-1細胞の分化に伴うPAF受容体の誘導とその性質　　　　　　　　　　　　　　　
1993

EOL-1細胞におけるPAF受容体　　　　　　　　　　　　　　　
Aug. 1992
Lead, (公社)日本生化学会

血小板活性化因子と情報伝達　　　　　　　　　　　　　　　
May 1992
Last

第８回国際プロスタグランジン会議見聞録　　　　　　　　　　　　　　　
1992
Lead

ヒト血小板のアラキドン酸12-リポキシゲナーゼのcDNAクローニング　　　　　　　　　　　　　　　
Aug. 1991
Lead, (公社)日本生化学会

ロイコトリエン(LT)A4の代謝 LTA4水解酵素とLTC4合成酵素の精製及び性質　　　　　　　　　　　　　　　
Jun. 1987
日本脂質生化学会

モルモット肺におけるロイコトリエンC4合成酵素　　　　　　　　　　　　　　　
Mar. 1987
Lead

ラット肺胞マクロファージのLeukotrieneB4産生能におよぼす喫煙の影響　　　　　　　　　　　　　　　
Mar. 1987

ロイコトリエン(LT)A4水解酵素 ー 人肺よりの精製　　　　　　　　　　　　　　　
Mar. 1987

生化学・細胞機能 2
1987

ロイコトリエン(LT)C4合成酵素 モルモットにおける臓器分布及び肺からの精製　　　　　　　　　　　　　　　
Aug. 1986
Lead, (公社)日本生化学会

マウスマスト細胞のカルシウム依存性5-リポキシゲナーゼの精製と性質　　　　　　　　　　　　　　　
Jun. 1986
日本脂質生化学会

モルモット全身臓器におけるLTA4水解(LTB4合成)酵素活性およびLTC4合成酵素活性の分布　　　　　　　　　　　　　　　
Jun. 1986
Lead, 日本脂質生化学会

ヒト顆粒球,マウス肥満細胞におけるロイコトリエンB4の代謝（ ω-酸化物との同時微量定量法の確立とその応用）　　　　　　　　　　　　　　　
Mar. 1986
Lead, (一社)日本呼吸器学会

モルモット肺及び主要臓器におけるロイコトリエンA4水解酵素（LTB4合成酵素）　　　　　　　　　　　　　　　
Mar. 1986

ロイコトリエンB4の微量定量系の確立とその応用　　　　　　　　　　　　　　　
Oct. 1985
Lead

マスト細胞,白血球におけるLTB4産生　　　　　　　　　　　　　　　
Aug. 1985
Lead

マスフラグメントメトリーによるロイコトリエンB4及びプロスタグランジンの微量定量　　　　　　　　　　　　　　　
Jul. 1985
Lead

珪肺に併発した肺門部早期肺癌の一切除例 : 関東支部 : 第78回日本肺癌学会関東支部会
30 Apr. 1984

モルモット肺におけるβ受容体数と気道のβ刺激剤に対する感受性(in vitro)について　　　　　　　　　　　　　　　
Mar. 1984
Lead, (一社)日本呼吸器学会

3 各種肺疾患患者の気管支肺胞洗浄液中ロイコトリエンの測定(生化学)
1984

気道
1984

びまん性細気管支炎
1984

喘息, 過敏性
1984

21.多発空洞形成を伴った原発性肺癌の一例(第77回日本肺癌学会関東支部会)
25 Dec. 1983

ラット肺α,β受容体数の加齢による変動について　　　　　　　　　　　　　　　
Mar. 1983
Lead, (一社)日本呼吸器学会

喘息発作誘発によるモルモット肺β受容体数の変動について　　　　　　　　　　　　　　　
Mar. 1983
Lead, (一社)日本呼吸器学会

ロイコトリエンと気道過敏性ーHPLCによる臨床的, 実験的研究ー　　　　　　　　　　　　　　　
1983

気管支喘息I
1983

呼吸調節V
1983

6.ホルモン療法が奏効した癌性リンパ管炎(乳癌原発)の1症例 : 第74回肺癌学会関東支部
25 Dec. 1982

気管支鏡による癌性リンパ管炎の診断
和泉 孝志
肺癌, 31 Jul. 1982
Lead, (NPO)日本肺癌学会

ラット肺におけるアドレナリン性β受容体数の測定加齢による変動について　　　　　　　　　　　　　　　
Mar. 1982
Lead

生化学的肺機能検査法にかんする基礎的検討(第3報)PGE1,PGF2α,Isoproterenolの全身血圧におよぼす影響について　　　　　　　　　　　　　　　
Mar. 1982
Lead

Direct β-adrenergic receptor assay of animal lung - Effects of various drugs. (Abstract)　　　　　　　　　　　　　　　
T IZUMI; LH HSU; Y SUGIYAMA; R HAYASHI; S KITAMURA
XIV World congress on the diseases of the chest and the 48th annual scientific assambly, 1982
Lead

各種肺疾患における経気管支肺生検の診断的意義について
1982
Lead, (NPO)日本呼吸器内視鏡学会

69 気管支拡張症の気管支造影像(造影)
1982

77 びまん性汎細気管支炎の内視鏡像と気道粘膜病理像(肉眼的所見と病理学的所見の一致性 (1))
1982

98 縦隔および肺のびまん性血管腫の 1 例(症例 (III))
1982

116 経気管支生検施行時の出血に対するトロンビン局所注入療法(気管支鏡検査による合併症)
1982

気道過敏性
1982

気道病態 感染症
1982

代謝・血管作動物質 I
1982

気管支喘息III
1982

気管支喘息VI
1982

多発性肺軟骨腫の1手術例
和泉孝志
肺癌, 30 Sep. 1981
Lead, (NPO)日本肺癌学会

経気管支肺生検により診断しえた好酸球性肉芽腫症の1例　　　　　　　　　　　　　　　
Aug. 1981
Lead

気管支収縮剤の作用におよぼすβ受容体遮断剤の効果について　　　　　　　　　　　　　　　
Apr. 1981
Lead

モルモット肺におけるDirect β-Adrenergic Receptor Assay(第2報) α受容体遮断剤,β受容体遮断剤,インフルエンザワクチン,百日咳ワクチン投与の影響について　　　　　　　　　　　　　　　
Apr. 1981
Lead, (一社)日本呼吸器学会

各種肺疾患における経気管支肺生検の診断的意義について　　　　　　　　　　　　　　　
Apr. 1981
Lead, (一社)日本呼吸器学会

吸引性肺炎の病態に関する実験的研究(第1報) イヌ気道系および肺循環系におよぼす影響を中心として　　　　　　　　　　　　　　　
Apr. 1981
Lead, (一社)日本呼吸器学会

癌性心膜炎のテトラサイクリン療法　　　　　　　　　　　　　　　
1981
(NPO)日本肺癌学会

Effect of prostaglandins on the anaphylactic release of histamine and slow reacting substance of anaphylaxis (SRS-A). (Abstract)　　　　　　　　　　　　　　　
S KITAMURA; Y ISHIHARA; T IZUMI; Y SUGIYAMA; R HAYASHI; LH HSU
Clin. Res., 1981

Effect of Ipratropium bromide (Sch 1000) on the action of various bronchoactive agents. (Abstract)　　　　　　　　　　　　　　　
S KITAMURA; Y ISHIHARA; T IZUMI; R HAYASHI; LH HSU; Y SUGIYAMA
Clin. Res., 1981

8 気管支造影後の副作用におよぼすステロイド剤および抗生物質の気管支腔内注入療法の効果について
1981

14 慢性気管支炎患者に対するトラネキサム酸の効果について : 気管支ファイバースコープを用いた臨床的研究
1981

16 気管支鏡による吸引性肺炎の診断と治療
1981
Lead

20 びまん性汎細気管支炎患者の気管支鏡所見と TBLB による組織診断について
1981

49 原発性肺癌患者に対する経気管支的多剤局所注入療法の検討
1981

ラ(1)-3 各種肺疾患における経気管支生検の診断的意義について
1981
Lead

気胸I
1981

腫瘍III
1981

慢性閉塞性肺疾患
1981

生検
1981

肺循環
1981

感染症I
1981

気道過敏性I
1981

代謝
1981

6.アミラーゼ産生肺癌の一剖検例 : 第68回肺癌学会関東支部
25 Dec. 1980
Lead

Gilbert症候群の1症例-肝,胆汁中のグルクロン酸抱合型ビリルビンについて　　　　　　　　　　　　　　　
Apr. 1980
Lead

ウサギ肺におけるDirectβ-AdrenergicReceptorAssay薬物投与の影響について　　　　　　　　　　　　　　　
Mar. 1980
Lead

気管支収縮剤の作用におよぼすβ受容体遮断剤の効果について
Mar. 1980
Lead, (一社)日本呼吸器学会

気管支喘息
1980

高カリウム血症,低カルシウム血症,高リン血症をきたして死亡した成人T-cell ALLの1例　　　　　　　　　　　　　　　
Dec. 1979
Lead

一卵性双生児の一方に下垂体性巨人症を,他方に先端巨大症を発症した兄弟例　　　　　　　　　　　　　　　
Mar. 1979

薬を選んで気管支喘息を直す　　　　　　　　　　　　　　　
1979

カラー生化学
Joint translation
Mar. 2015
9784890134502

脂質生物学がわかる―脂質メディエーターの機能からシグナル伝達まで (わかる実験医学シリーズ―基本&トピックス)
Contributor
01 Oct. 2004
4897069653

呼吸器疾患を探る 非腫瘍編 (シミュレイション内科)（河野修興編）
Contributor
Jun. 2004
4815916705

ストライヤー生化学
Joint translation
Jan. 2004
4807905813

ポストシークエンスタンパク質実験法４ 構造・機能解析の実際（大島泰郎・鈴木紘一・藤井義明・村松喬 編）　　　　　　　　　　　　　　　
Contributor
Mar. 2003
4807911376

麻酔管理に関連する受容体（編集 大下修造）　　　　　　　　　　　　　　　
Contributor
2003
4880037036

Oxygen and life : oxygenases, oxidases and lipid mediators : proceedings of the 3rd International Conference on Oxygen and Life which was held in Kyoto, between 26 and 29 November 2000./ editors, Yuzuru Ishimura ... [et al.]
Shimizu, T; Yokomizo, T; Izumi, T, Contributor, Characterizationof two distinct types of leukotriene B4 receptors. pp 415-420
Elsevier, 01 Dec. 2002
0444508724

これだけは知っておきたいアラキドン酸カスケードQ&A（室田 誠逸編）
Contributor
Jul. 2002
4753219755

血小板と生理活性脂質　　　　　　　　　　　　　　　
Contributor
2002

ロイコトリエンB4受容体の生体内・細胞内における役割の解明
Mar. 2001

肺への好中球集積機構の解析 : LTB[4]受容体発現CHO細胞を用いた研究
Mar. 2001

炎症性生理活性脂質受容体の機能制御
Mar. 2000

新・内科治療ガイド（矢崎義雄, 和田 攻, 大久保明行, 永田直一編）　　　　　　　　　　　　　　　
Contributor
Apr. 1999

ロイコトリエン受容体のクローニングと細胞内情報伝達機構
Mar. 1999

Eicosanoidsと気管支喘息（The 4th Symposium of Asthma in Tokyo、富岡玖夫, 伊藤幸治編）　　　　　　　　　　　　　　　
Contributor
Apr. 1997

生物薬科科学実験講座（市川厚, 岡孝巳編）　　　　　　　　　　　　　　　
Contributor
1996

生理活性脂質の生合成と作用機序
Mar. 1994

Molecular Basis of Inflammation Vol.3 (edited by J. Navarro)　　　　　　　　　　　　　　　
T. Shimizu; H. Mutoh; I. Waga; M. Nakamura; Z. Honda; T. Izumi, Contributor, Structure and function of platelet-activating factor receptor
Ares-Serono Symposia Publications, 1994

Advances in Polyunsaturated Fatty Acids Research (edited by T. Yasugi, H. Nakamura, M. Soma)　　　　　　　　　　　　　　　
T. Shimizu; M. Minami; Y. Sawada; T. Izumi, Contributor, Bifunctional Roles of Leukotriene A4 Hydrolase
Elsevier, 1993

ロイコトリエン受容体と情報伝達に関する研究
1993

新生化学実験講座 第９巻ホルモンII, 非ペプチドホルモン（日本生化学会編）
Contributor
Dec. 1992
4807910752

内科学（疾患篇）（小坂樹徳編）　　　　　　　　　　　　　　　
Contributor
Jan. 1988

血小板1987ｰ血小板と脂質 （血小板芦ノ湖カンファレンス）　　　　　　　　　　　　　　　
Contributor
1987

現代化学・増刊7、プロスタグランディン研究法（下）　　　　　　　　　　　　　　　
Contributor
1987

現代化学・増刊６、プロスタグランディン研究法（上）　　　　　　　　　　　　　　　
Contributor
1986

Design and Development of Silicon Nanowire Biosensor with Enhanced Sensitivity and Specificity for Biomolecule Detection　　　　　　　　　　　　　　　
Hui Zhang; Fumiya Osawa; Yawei Qiu; Haru Okamoto; Zhiheng Liu; Noriyasu Ohshima; Toshiya Sakata; Taira Kajisa; Takashi Izumi; Hayato Sone
5th Gunma International Symposium 2025 (GIS2025), 23 Jan. 2025

Enhancing Detection Sensitivity of Si Nanowire Biosensor Through Controlled Channel Impurity Concentrations　　　　　　　　　　　　　　　
Fumiya Osawa; Hui Zhang; Yawei Qiu; Noriyasu Ohshima; Toshiya Sakata; Taira Kajisa; Takashi Izumi; Hayato Sone
5th Gunma International Symposium 2025 (GIS2025), 23 Jan. 2025

Si ナノワイヤバイオセンサの不純物濃度制御と電気特性評価　　　　　　　　　　　　　　　
09 Dec. 2024

Si ナノワイヤバイオセンサ作製のための熱酸化によるSi 層膜厚制御　　　　　　　　　　　　　　　
09 Dec. 2024

Si ナノワイヤバイオセンサ高感度化を目指した幅及び半導体極性効果の検証　　　　　　　　　　　　　　　
09 Dec. 2024

Ultrasensitive Specific Detection of Anti-influenza A H1N1 Hemagglutinin Monoclonal Antibody Using Silicon Nanowire Field Effect Biosensors　　　　　　　　　　　　　　　
Hui Zhang; Fumiya Osawa; Haru Okamoto; Yawei Qiu; Zhiheng Liu; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
37th International Microprocesses and Nanotechnology Conference (MNC2024), 14 Nov. 2024

Challenges in Forming Sub-10 nm Nanowires via Directed Self-Assembly for Future Applications in Silicon Nanowire Biosensor　　　　　　　　　　　　　　　
13 Nov. 2024

分岐鎖アミノ酸代謝と細胞老化・個体老化の関係　　　　　　　　　　　　　　　
Nov. 2024

COMSOL有限要素解析法を用いた高感度Siナノワイヤバイオセンサの最適な構造の予測　　　　　　　　　　　　　　　
19 Sep. 2024

分岐鎖アミノ酸代謝の変調が細胞老化及び個体老化に与える影響　　　　　　　　　　　　　　　
15 Jun. 2024

The Possibility of Ultrasensitive Detection of Biomolecules using Silicon Nanowire Biosensor with Structural Optimization　　　　　　　　　　　　　　　
16 Nov. 2023

テロメア短縮系を用いた効率的な複製老化系の樹立および分岐鎖アミノ酸代謝の細胞老化における役割の解析　　　　　　　　　　　　　　　
31 Oct. 2023

非接触型導電率検出キャピラリー電気泳動を用いた寒冷ストレス下における唾液イオンの網羅的解析　　　　　　　　　　　　　　　
Nov. 2023

Siナノワイヤバイオセンサ電気特性と検出感度の幅及び極性依存性　　　　　　　　　　　　　　　
21 Sep. 2023

Siナノワイヤバイオセンサ空乏領域の推測による高感度化要因の解明　　　　　　　　　　　　　　　
21 Sep. 2023

骨芽細胞様細胞株MC3T3-E1におけるPGE2分泌を介したメカノストレス伝播機構の解析　　　　　　　　　　　　　　　
08 Jun. 2023

マウス由来GDE7は環状ホスファチジン酸を産生する酵素活性を有する　　　　　　　　　　　　　　　
Jun. 2022

高感度Siナノワイヤバイオセンサ作製のための最適なドーピング濃度の探求　　　　　　　　　　　　　　　
16 Dec. 2021

電子線描画法を用いた超高感度 Siナノワイヤバイオセンサの作製と特性評価　　　　　　　　　　　　　　　
16 Dec. 2021

Development of rapid and efficient induction system for replicative senescence by mimicking telomere shortening　　　　　　　　　　　　　　　
Yuma Aramaki; Kazuki Irie; Aiko Tsuchida; Takashi Izumi; Yoji A. Minamishima; Akimitsu Konishi
14 Dec. 2021

分岐鎖アミノ酸代謝の変調による細胞老化制御機構　　　　　　　　　　　　　　　
Dec. 2021

分岐鎖アミノ酸代謝の変調による細胞老化制御機構　　　　　　　　　　　　　　　
19 Jun. 2021

MC3T3-E1における、脂質オートクライン刺激とエストラジオール刺激のクロストーク機構の解析　　　　　　　　　　　　　　　
Dec. 2020

キャピラリー電気泳動を用いる唾液中の陰及び陽イオンの同時分析と生理活性物質との相関　　　　　　　　　　　　　　　
13 Dec. 2019

骨芽細胞様細胞MC3T3-E1の細胞内外の脂質メディエーターがアクチン細胞骨格変化を介して骨形成を制御する　　　　　　　　　　　　　　　
06 Dec. 2019

Fabrication of Ultrahigh-Sensitivity Silicon Nanowire Biosensor by CMOS-Compatible Process for Detecting Biomolecules at Low Concentrations　　　　　　　　　　　　　　　
Hui Zhang; Kei Okabe; Naoki Kikuchi; Noriyasu Ohshima; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
International Conference on Mechanical, Electrical and Medical Intelligent System 2019, 04 Dec. 2019

シリコンナノワイヤ細線化によるバイオセンサ検出感度の評価　　　　　　　　　　　　　　　
18 Sep. 2019

LipidBankデータベースの歴史と現状　　　　　　　　　　　　　　　
Jun. 2019

家庭環境内における重金属類曝露シナリオの解明－調査方法の検討　　　　　　　　　　　　　　　
Jun. 2019

電子線描画法による高感度シリコンナノワイヤバイオセンサの作製 およびアトモル濃度の抗原抗体特異結合の検出　　　　　　　　　　　　　　　
09 Mar. 2019

家庭環境内における重金属類暴露シナリオの解明ー調査方法の確立　　　　　　　　　　　　　　　
Feb. 2019

Fabrication of Highly Sensitive Silicon Nanowire Biosensor by Electron Beam Lithography for Detecting Antigen-Antibody Specific Binding at Attomolar Concentration　　　　　　　　　　　　　　　
Hui Zhang; Noriyasu Ohshima; Kakeru Oshima; Naoki Kikuchi; Taira Kajisa; Toshiya Sakata; Takashi Izumi; Hayato Sone
GUMI 2018 (5th International Symposium of Gunma University Medical Innovation), 06 Dec. 2018

Novel S1P chaperone activity in the plasma from ApoM/albumin-double knockout mice　　　　　　　　　　　　　　　
Nov. 2018

酸化脂質膜受容体であるヒトG2Aは脳腫瘍細胞の上皮間葉転換様プロセスを制御する　　　　　　　　　　　　　　　
27 May 2018

一般家庭における重金属曝露メカニズムの解明-調査プロトコルについて　　　　　　　　　　　　　　　
Mar. 2018

ゲノム編集技術を用いた蛍光蛋白ラベル法による内在性p53蛋白動態の1細胞解析　　　　　　　　　　　　　　　
21 Dec. 2017

骨芽細胞様細胞MC3T3-E1における分化段階に応じた機械的刺激による骨形成制御応答の解析　　　　　　　　　　　　　　　
07 Dec. 2017

膜貫通型リゾホスホリパーゼD、GDE4過剰発現細胞における脂質の網羅的解析　　　　　　　　　　　　　　　
07 Dec. 2017

ゲノム編集技術を用いた蛍光蛋白ラベル法による内在性p53蛋白動態の1細胞解析　　　　　　　　　　　　　　　
07 Dec. 2017

脂質データベースLipidBank のWiki版構築とその特徴　　　　　　　　　　　　　　　
Dec. 2017

スフィンゴシン１リン酸の新規担体分子の探索　　　　　　　　　　　　　　　
Dec. 2017

骨芽細胞様細胞MC3T3-E1における分化段階に応じた機械的刺激による骨形成制御応答　　　　　　　　　　　　　　　
Dec. 2017

Fabrication of Silicon Nanowire Biosensor Using Electron Beam Lithography and Low-Concentration Antibody Detection　　　　　　　　　　　　　　　
Yuya Sakurai; Tomoya Tashiro; Noriyasu Oshima; Kakeru Oshima; Sumio Hosaka; Takashi Izumi; Hayato Sone
4th International Symposium of Gunma University Medical Innovation (GUMI2017), Nov. 2017

電子線リソグラフィを用いたSiナノワイヤバイオセンサの作製と低濃度抗体検出　　　　　　　　　　　　　　　
Oct. 2017

An oxidazed lipids-membrane receptor, human G2A regulates epithelial-mesenchymal transition-like proces of glioma cells.　　　　　　　　　　　　　　　
30 Sep. 2017

質量分析によるメタボローム解析の現状と課題　　　　　　　　　　　　　　　
Jul. 2017, [Invited]

化学物質曝露評価における定量的メタボロミクスの現状と課題　　　　　　　　　　　　　　　
May 2017

電子線リソグラフを用いた超高感度Siナノワイヤバイオセンサの創製　　　　　　　　　　　　　　　
Mar. 2017

TRF2はコアヒストンとの直接結合によりテロメアのループ構造を維持する　　　　　　　　　　　　　　　
11 Jan. 2017

テロメア機能不全時に染色体末端に誘導されるDNA損傷応答の細胞周期依存的な制御　　　　　　　　　　　　　　　
01 Dec. 2016

Fabrication of N-type Silicon Nanowire Using Electron Beam Lithography for Antibody Detection　　　　　　　　　　　　　　　
Yuya Sakurai; Tomoya Tashiro; Noriyasu Oshima; Kakeru Oshima; Sumio Hosaka; Takashi Izumi; Hayato Sone
3rd International Symposium of Gunma University Medical Innovation and 8th International Conference on Advanced Micro‐Device Engineering (GUMI&AMDE 2016), Dec. 2016

可溶性2-アラキドノイルグリセロール産生酵素、DDHD2の酵素学的解析　　　　　　　　　　　　　　　
Jun. 2016

Fabrication of N-type Silicon Nanowire Using Electron Beam Lithography for Antibody Detection　　　　　　　　　　　　　　　
Mar. 2016

Cell cycle-dependent regulation of non-homologous end joining at unprotected telomeres　　　　　　　　　　　　　　　
第３３回染色体ワークショップ, Jan. 2016

Complete sequence of human telomere DNA by single-molecule real-time sequencing technique　　　　　　　　　　　　　　　
08 Dec. 2015

Cell cycle-dependent regulation of non-homologous end joining at unprotected telomeres　　　　　　　　　　　　　　　
02 Dec. 2015

Glycosphingolipids Database in LipidBank Wiki Version　　　　　　　　　　　　　　　
Dec. 2015

Fabrication of N-type Silicon Nanowire Using Electron Beam Lithography for Antibody Detection　　　　　　　　　　　　　　　
Yuya Sakurai; Tomoya Tashiro; Noriyasu Oshima; Kakeru Oshima; Sumio Hosaka; Takashi Izumi; Hayato Sone
2nd International Symposium of Gunma University Medical Innovation (GUMI2015), Dec. 2015

Purification and characterization of a soluble diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
Araki M.; Aso C.; Ohshima N.; Tatei K.; Hirano T.; Sugimoto S.; Kishi M.; Kishimoto K.; Obinata H.; Izumi T.
14th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases, Jul. 2015

Gタンパク質共役型受容体であるG2Aはがん細胞の幹細胞性を制御する　　　　　　　　　　　　　　　
29 May 2015

新規リゾホスファチジン酸産生酵素、GDE4・GDE7の酵素学的解析　　　　　　　　　　　　　　　
May 2015

GDE4 and GDE7 Produce Lysophosphatidic Acid by Lysophospholipase D Activity　　　　　　　　　　　　　　　
Mari Araki; Chizu Aso; Noriyasu Ohshima; Kazuaki Tatei; Tohko Hirano; Hiroyuki Sugimoto; Mikiko Kishi; Koji Kishimoto; Takashi Izumi
The 6th international conference on Phospholipase A2 and Lipid Mediators (PLM 2015), 09 Feb. 2015, [Invited]

Identification and functional analyses of a novel diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
Chiaki Isaji; Takahiro Kudo; Noriyasu Ohshima; Yosuke Yamashita; Mari Araki; Ayako Honda; Tomomi Nagano; Norihisa Kato; Akiyoshi Konishi; Koji Kishimoto; Kazuaki Tatei; Noriyuki Yanaka; Takashi Izumi
The 6th international conference on Phospholipase A2 and Lipid Mediators (PLM 2015), 09 Feb. 2015, [Invited]

新規酵素GDE4およびGDE7は，LPAおよびalkyl-LPAの産生に関与する　　　　　　　　　　　　　　　
Jan. 2015

Cell Cycle-Dependent Regulation of DNA Damage Response and DNA Repair at Dysfunctional Telomeres.　　　　　　　　　　　　　　　
The 4D Nucleome 2014, 18 Dec. 2014

Fabrication of N-type Silicon Nanowire Using FIB for Biomolecule Detection　　　　　　　　　　　　　　　
Hayato Sone; Yuya Sakurai; Takashi Izumi; Sumio Hosaka
1st International Symposium of Gunma University Medical Innovation and 6th International Conference on Advanced Micro-Device Engineering (GUMI＆AMDE2014), 05 Dec. 2014

テロメアにおける細胞周期依存的なDNA損傷反応・DNA修復の制御機構　　　　　　　　　　　　　　　
Dec. 2014

テロメアでの細胞周期依存的なDNA損傷反応・DNA修復反応の制御機構　　　　　　　　　　　　　　　
27 Nov. 2014

酸化脂質をリガンドとするGPCR, G2Aは幹細胞性制御に関わる　　　　　　　　　　　　　　　
Nov. 2014

ラット脳ジアシルグリセロールリパーゼの精製と機能解析　　　　　　　　　　　　　　　
07 Aug. 2014

群馬大学におけるAdvanced OSCE の試み；医療コミュニケーション能力の評価を目的とした新規課題の導入　　　　　　　　　　　　　　　
Jul. 2014

Gタンパク質共役型受容体、G2Aが制御するがん幹細胞性と上皮間葉転換　　　　　　　　　　　　　　　
Jul. 2014, [Invited]

LipidBankにおける糖脂質分類：植物編　　　　　　　　　　　　　　　
Jun. 2014

Identification and functional analyses of a novel diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
2014

Regulation of DNA damage checkpoint termination　　　　　　　　　　　　　　　
The 36th Annual Meeting of the Molecular Biology Society of Japan, 03 Dec. 2013

Wee1によるDNA損傷チェックポイントの回復制御　　　　　　　　　　　　　　　
25 Nov. 2013

9-HODE-activated GPCR, G2A Regulates Stemness of Cancer Stem Cells in Human Astrocytoma Cell Lines　　　　　　　　　　　　　　　
Kishimoto K; Haraguchi T; Shimizu K; Yamaguchi A; Yoshihara T; Ohshima N; Tatei T; Tobita S; ida Y; Tsushima Y; Izumi T
The Scripps Research Institute (San Diego), Nov. 2013, [Invited]

9-HODE-activated GPCR, G2A induces epithelial-to-mesenchymal transition of human astrocytoma cells prior to generation of stem cell-like properties.　　　　　　　　　　　　　　　
Kishimoto K.; Haraguchi T.; Shimizu K.; Yamaguchi A.; Yoshihara T.; Ohshima N.; Tatei K.; Tobita S.; Tsushima Y.; Izumi T.
Society of Neuroscience 2013, Nov. 2013

9-HODE-activated G2A induces epithelial-to-mesenchymal transition of human astrocytoma cells prior to generation of stem cell-like properties.　　　　　　　　　　　　　　　
Kishimoto K.; Haraguchi T.; Shimizu K.; Yamaguchi A.; Yoshihara T.; Ohshima N.; Tatei K.; Tobita S.; Tsushima Y.; Izumi T.
Society of Neuroscience 2013, Nov. 2013

Differentiation of malignant tumors from granulomas with dynamic [18F]-Fluoro-L-a-methyltyrosine PET imaging　　　　　　　　　　　　　　　
Yamaguchi A; Iida Y; Fujisawa Y; Inamoto S; Zhao S; Suzue K; Tominaga H; Kishimoto K; Higuchi T; Hisaeda H; Izumi T; Kuge Y; Tsushima Y
European Association of Nuclear Medicine (Lyon), Oct. 2013

Identification of a novel diacylglycerol lipase from rat brain　　　　　　　　　　　　　　　
Sep. 2013

血管平滑筋細胞に対するニコチンの分化・脱分化作用に関する解析　　　　　　　　　　　　　　　
Sep. 2013

Gタンパク質共役型受容体、G2Aはアストロサイトーマ細胞株のがん幹細胞性を制御する　　　　　　　　　　　　　　　
Sep. 2013

皮膚科学におけるG2Aの役割　　　　　　　　　　　　　　　
22 Jul. 2013

基礎学力の向上を目指す教養教育科目「学びのリテラシー」の実践　　　　　　　　　　　　　　　
Jul. 2013

LipidBankにおけるArchaea脂質のデータベース　　　　　　　　　　　　　　　
Jul. 2013

Gタンパク質共役型受容体，G2Aはヒトアストロサイトーマ細胞株のがん幹細胞性を制御する　　　　　　　　　　　　　　　
Jun. 2013, [Invited]

Subcellular distribution and binding specificity of phospholipid-binding proteins　　　　　　　　　　　　　　　
Xi Xin; Kazuaki Tatei; Hideaki Ehara; Yumiko Kihara; Takashi Izumi
JCBL (The Japanese Conference on the Biochemistry of Lipids) 55, Jun. 2013

新規脳ジアシルグリセロールリパーゼの同定　　　　　　　　　　　　　　　
Jun. 2013

発光測定に基づく低酸素細胞・組織イメージング技術の開発と医学応用　　　　　　　　　　　　　　　
Apr. 2013

新しいLipidBankにおける糖脂質分類
Jun. 2013

アストロサイトーマU251細胞の幹細胞性と上皮間葉転換制御に関わるGタンパク質共役型受容体G2Aの役割　　　　　　　　　　　　　　　
Dec. 2012

抗ロイコトリエンC4モノクローナル抗体とその単鎖抗体によるロイコトリエン中和作用　　　　　　　　　　　　　　　
Dec. 2012

ロイコトリエンに対する単鎖抗体を利用した抗体治療薬開発に向けた基礎研究　　　　　　　　　　　　　　　
Nov. 2012

Oxidized Free Fatty Acids-Activated GPCR, G2A Regulates Stemness and Malignant Behavior of An Astrocytoma Cell Line　　　　　　　　　　　　　　　
Neuroscience 2012, Oct. 2012

G2A Regulates Stemness and malignant behavior of Astrocytoma Cells.　　　　　　　　　　　　　　　
Sep. 2012

アンケート調査からみた生涯教育への臨床医のニーズ ー群馬大学医学教育センターリカレント教育部門の活動ー　　　　　　　　　　　　　　　
Jul. 2012

表面プラズモン共鳴現象を利用した効率的なGPCRリガンドスクリーニング方法
23 Jun. 2012

がん幹細胞性制御におけるG2Aの役割　　　　　　　　　　　　　　　
Jun. 2012, [Invited]

酸化遊離脂肪酸をリガンドとするGタンパク質共役型受容体、G2Aはアストロサイトーマ細胞株のがん幹細胞性を制御し、悪性度を規定する　　　　　　　　　　　　　　　
Jun. 2012

ロイコトリエン作用に対する抗ロイコトリエンC4単鎖抗体の中和効果　　　　　　　　　　　　　　　
May 2012

Gタンパク質共役型受容体、G2Aを介したがん幹細胞性制御　　　　　　　　　　　　　　　
May 2012

A stimulus-dependent, dentate gyrus-specific novel phospholipase A2　　　　　　　　　　　　　　　
Nov. 2011

グリオーマ細胞におけるG2Aを介した形質変化の制御　　　　　　　　　　　　　　　
Nov. 2011, [Invited]

G2Aは神経膠腫細胞株U251に栄養飢餓及び低酸素刺激に対する抵抗性を賦与する　　　　　　　　　　　　　　　
Sep. 2011

Expression of a single-chain antibody that neutralizes the effect of cysteinyl leukotrienes　　　　　　　　　　　　　　　
Takahashi Y; Kawakami Y; Hirano S; Kinoshita M; Otsuki A; Yamamoto TS; Kurahashi Y; Daiyasu H; Toh H; Kishimoto K; Izumi T; Yamamoto S
52nd International Conference on the Bioscience of Lipids, 30 Aug. 2011, [Invited]

哺乳動物のGDE群とマウスGDE5の非酵素的機構による骨格筋分化制御　　　　　　　　　　　　　　　
Aug. 2011

生理活性脂質研究の新展開　　　　　　　　　　　　　　　
Jun. 2011

哺乳動物のGDE群とマウスGDE5の非酵素的機構による骨格筋分化制御
May 2011

酪酸ナトリウムでラット肝臓幹様細胞のAnnexin A3 の発現が抑制される　　　　　　　　　　　　　　　
Dec. 2010

マウス由来グリセロホスホジエステルホスホジエステラーゼ１(GDE1)の基質特異性と組織分布の解析　　　　　　　　　　　　　　　
Dec. 2010

pColdベクターを用いた5-リポキシゲナーゼ(5-LO)の大量発現と精製　　　　　　　　　　　　　　　
Dec. 2010

Cytosolic phospholipase A2 alpha amplifies early cyclooxygenase-2 expression, oxidative stress and MAP kinase phosphorylation after cerebral ischemia in mice　　　　　　　　　　　　　　　
Dec. 2010

pColdベクターを用いた5-リポキシゲナーゼ (5-LO)の大量発現と精製　　　　　　　　　　　　　　　
Nov. 2010

酸化遊離脂肪酸をリガンドとするG2Aの機能とサブタイプの解析　　　　　　　　　　　　　　　
May 2010

アシルプロテインチオエステラーゼ1/リゾホスホリパーゼ1の活性と細胞内局在　　　　　　　　　　　　　　　
May 2010

Novel function of 9(S)-hydroxyoctadecadienoic acid on proliferation of T cells　　　　　　　　　　　　　　　
May 2010

Ceramide induces myogenic differentiation and apoptosis in Drosophila Schneider cells.　　　　　　　　　　　　　　　
Dec. 2009

Functional role of a novel glycerophosphodiester phosphodiesterase GDE5 during myoblast differentiation.　　　　　　　　　　　　　　　
Dec. 2009

ほ乳類グリセロホスホジエステルホスホジエステラーゼの酵素学的解析　　　　　　　　　　　　　　　
Nov. 2009

9-HODE induces cell growth arrest in Jurkat T lymphocytes.　　　　　　　　　　　　　　　
Oct. 2009

Proteomic analysis of rat hepatic stem-like cells respond to sodium butyrate reversibly.　　　　　　　　　　　　　　　
Sep. 2009

Detection of Receptor protein tyrosine kinase-mediated cytoskeletal rearrangement in living cells using surface plasmon resonance.　　　　　　　　　　　　　　　
Sep. 2009

High methionine diet-induced acute hepatitis in CSE-deficient mice.　　　　　　　　　　　　　　　
Sep. 2009

ショウジョウバエ培養細胞Schneider株においてセラミドは筋分化とアポトーシスを誘導する
Jul. 2009

表面プラズモン共鳴現象を用いた生細胞におけるGPCRシグナルの検出　　　　　　　　　　　　　　　
Jul. 2009

The transcriptional regulation of Ets2 and CTP:phosphocholine cytidylyltransferase alfa in M-CSF stimulated RAW264 osteoclast precursor cells.　　　　　　　　　　　　　　　
Okamura K.; Takagishi K.; Yamashita S.; Aoyama C.; Horibata Y.; Izumi T.; Vance D.; Sugimoto H.
55th Annual Meeting of the Orthopaedic Research Societies, May 2009

Characterization of mouse glycerophosphodiester phosphodiesterase 1 (GDE1)　　　　　　　　　　　　　　　
Noriyasu Ohshima; Mayumi Kaneko; Naoko Takahashi; Koji Takio; Yoshitsugu Shiro; Kazuaki, Tatei; Takashi Izumi
4th International Conference on Phospholipase A2 and Lipid Mediators, May 2009

Ceramide induces myogenic differentiation and apoptosis in Drosophila Schneider cells　　　　　　　　　　　　　　　
Kazuaki Tatei; Hidemasa Kawamura; Tetsuo Nonaka; Hideru Obinata; Tomoyasu Hattori; Ai Ogawa; Hideko Kazama; Nobuyuki Hamada; Tomoo Funayama; Tetsuya Sakashita; Yasuhiko Kobayashi; Takashi Nakano; Takashi Izumi
4th International Conference on Phospholipase A2 and Lipid Mediators, May 2009

Oxidized free fatty acids mediate alarm signals through a G-protein coupled receptor, G2A　　　　　　　　　　　　　　　
Takashi Izumi; Hideru Obinata; Ai Ogawa; Tomoyasu Hattori; Mikiko Kishi; Noriyasu Ohshima; Kexin Chen; Melisa Intan Barliana; Kazuaki Tatei
4th International Conference on Phospholipase A2 and Lipid Mediators, May 2009

新規因子GDE5の筋芽細胞分化における役割、および骨格筋特異的GDE5過剰発現マウスの形質の解析　　　　　　　　　　　　　　　
Mar. 2009

Characterization of a neutral sphingomyelinase homolog as a phosphodiesterase for phosphoethanolamine ceramide in Drosophila cells　　　　　　　　　　　　　　　
Kazuaki TATEI; Hidemasa KAWAMURA; Tetsuo NONAKA; Hideru OBINATA; Tomoyasu HATTORI; Ai OGAWA; Hideko KAZAMA; Nobuyuki HAMADA; Tomoo FUNAYAMA; Tetsuya SAKASHITA; Yasuhiko KOBAYASHI; Takashi NAKANO; Takashi IZUMI
4th COE International Symposium, Nov. 2008

STUDY OF BUTYRATE SIGNAL TRANSDUCTION PATHWAYS IN RATHEPATIC STEM-LIKE CELLS　　　　　　　　　　　　　　　
Nov. 2008

Gタンパク質共役型受容体G2Aのリガンド同定と機能解析　　　　　　　　　　　　　　　
Jul. 2008

マウス由来グリセロホスホジエステルホスホジエステラーゼ１(GDE1)の酵素学的解析　　　　　　　　　　　　　　　
Jun. 2008

リゾホスホリパーゼ1の脱パルミトイル化活性の解析　　　　　　　　　　　　　　　
Jun. 2008

酸化遊離脂肪酸をリガンドとするG2Aの生理機能　　　　　　　　　　　　　　　
Jun. 2008

Gタンパク質共役型受容体G2Aの新規スプライシングバリアントの同定と機能解析　　　　　　　　　　　　　　　
Jun. 2008

9-hydroxyoctadecadienoic acid, one of the oxidized metabolites of linoleic acid, induces cell cycle arrest in the G1 phase in primary human keratinocytes　　　　　　　　　　　　　　　
Hideru Obinata; Takashi Izumi
International Workshop on Cell Regulation in Division and Arrest Under Stress, Apr. 2008

脱パルミトイル化酵素としてのリゾホスホリパーゼ1の解析　　　　　　　　　　　　　　　
Dec. 2007

Ets2の核内(NLS)および核外移行シグナル(NES)の同定およびRAW293細胞を用いたM-CSF刺激によるCTP:phosphocholine cytidylyltransferaseα(CTα)転写促進におけるEts2の重要性　　　　　　　　　　　　　　　
Dec. 2007

生細胞におけるGPCR活性の表面プラズモン共鳴(SPR)による観察　　　　　　　　　　　　　　　
Dec. 2007

ラット肝臓幹様（HSL）細胞を酪酸処理したときのProhibitin の応答　　　　　　　　　　　　　　　
Dec. 2007

Gタンパク質共役型受容体G2Aのスプライシングバリアントの同定と機能解析　　　　　　　　　　　　　　　
Dec. 2007

表皮角化細胞におけるGタンパク質共役型受容体G2Aの生理作用　　　　　　　　　　　　　　　
Jun. 2007

G2A MEDIATES SIGNALS OF OXIDIZED FREE FATTY ACIDS IN HUMAN KERATINOCYTES　　　　　　　　　　　　　　　
Hideru Obinata; Tomoyasu Hattori; Ai Ogawa; Kazuaki Tatei; Takashi Izumi
3rd International Conference on Phospholipases A2 and Lipid Mediators, May 2007

OXIDIZED FREE FATTY ACID-G2A SIGNALING MEDIATES ALARM SIGNAL UNDER OXIDATIVE STRESS　　　　　　　　　　　　　　　
Takashi Izumi; Hideru Obinata; Tomoyasu Hattori; Ai Ogawa; Kexin Chen; Melisa Intan Barliana; Mikiko Kishi; Kazuaki Tatei
3rd International Conference on Phospholipases A2 and Lipid Mediators, May 2007

ロイコトリエン研究の最近の進歩　　　　　　　　　　　　　　　
Mar. 2007

Gタンパク質共役型受容体G2Aのリガンド同定と皮膚における生理機能の解析　　　　　　　　　　　　　　　
Oct. 2006

Gタンパク質共役型受容体G2Aのリガンド同定とシグナル解析　　　　　　　　　　　　　　　
Jun. 2006

質量分析法を用いたリゾホスホリパーゼによるパルミトイル化ペプチドの検出
Jun. 2006

Bioluminescence Resonance Energy Transfer によるロイコトリエンB4受容体二量体の検出　　　　　　　　　　　　　　　
Jun. 2006

ショウジョウバエ初期胚における放射線誘導性アポトーシスの解析　　　　　　　　　　　　　　　
Jun. 2006

Proteome analysis of hepatocyte stem-like (HSL) cell differentiation.　　　　　　　　　　　　　　　
Toshihiko Saheki; Hitomi Ito; Naoyuki Takayama; Kentaro Nakamura; Akihiro Sekiguchi; Atsuyoshi Nishina; Takashi Izumi; Toshihiro Sugiyama; Masaharu Seno; Itaru Kojima
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006

Cystathionine gamma-lyase-deficient mice as animal models for cystathioninaemia　　　　　　　　　　　　　　　
Noriyuki Akahoshi; Takashi Izumi; Yasuki Ishizaki; Isao Ishii
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006

Partial purification of natural ligands for orphan G-protein coupled receptors　　　　　　　　　　　　　　　
Takashi Tujii; Hideru Obinata; Takashi Izumi; Shigeki Takeda
20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress, Jun. 2006

Purification and characterization of lysophospholipase D from rat brain　　　　　　　　　　　　　　　
Sayaka Sugimoto; Hiroyuki Sugimoto; Chizu Aso; Takashi Izumi
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Jun. 2006

Human G2A is a G protein-coupled receptor activated by 9-hydroxyoctadecadienoic acid and other oxidized free fatty acids　　　　　　　　　　　　　　　
Hideru Obinata; Tomoyasu Hattori; Kazuaki Tatei; Takashi Izumi
20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress, Jun. 2006

Detection of GPCR activity by surface plasmon resonance (SPR) in living cells　　　　　　　　　　　　　　　
Chen Kexin; Hideru Obinata; Kohei Hosaka; Takashi Izumi
20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress, Jun. 2006

Identification of nuclear-export-signal(NES) and nuclear-localization-signal(NLS) of Ets-2,and their relationship to the transcriptional regulation of CTP:phosphocholine Cytidylyltransferase α　　　　　　　　　　　　　　　
Koichi Okamura; Takashi Izumi; Kenji Takagishi; Dennis E. Vance; Hiroyuki Sugimoto
20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress, Jun. 2006

Lysophospholipase is a deacylating enzyme useful for the detection of palmitoylated peptide by MALDI-TOF mass spectrometry　　　　　　　　　　　　　　　
Tohko HIRANO; Merisa I BARLIANA; KISHI MIKIKO; Hiroyuki SUGIMOTO; Chie MURATA; Ryo TAGUCHI; IZUMI TAKASHI
20th IUBMB International Congress of Biochemistry and Molecular Bioligy and 11th FAOBMB Congress, Jun. 2006

The roles of G2A in human keratinocytes as a receptor for 9-hydroxyoctadecadienoic acid　　　　　　　　　　　　　　　
Tomoyasu Hattori; Hideru Obinata; Ai Ogawa; Kazuaki Tatei; Osamu Ishikawa; Takashi Izumi
20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress, Jun. 2006

酸化遊離脂肪酸の受容体G2Aのヒト表皮角化細胞における生理的役割　　　　　　　　　　　　　　　
May 2006

Detection of GPCR activity by surface plasmon resonance (SPR) in living cells　　　　　　　　　　　　　　　
Chen K.; Obinata H.; Izumi T.
Keystone Symposia, G Protein-Coupled Receptors: Evolving Concepts and New Tchniques, Feb. 2006

Analysis of the dimerization/ oligomerization of Leukotriene B4 receptors by BRET　　　　　　　　　　　　　　　
Kishi M; Obinata H; Barliana M I; Izumi T
Keystone Symposia, G Protein-Coupled Receptors: Evolving Concepts and New Tchniques, Feb. 2006

ショウジョウバエの初期胚に対する重粒子線照射の影響　　　　　　　　　　　　　　　
Jan. 2006

A biological role of G2A in human keratinocytes as a receptor for oxidized free fatty acids　　　　　　　　　　　　　　　
Hattori, T.; Obinata, H.; Ogawa, A.; Izumi, T.
Keystone Symposia, Eicosanoid in Inflammation and Chronic Disease, Jan. 2006

Identification of 9-hydroxyoctadecadienoic acid and other oxidized free fatty acids as ligands of the G-protein coupled receptor G2A　　　　　　　　　　　　　　　
Obinata, H.; Hattori, T.; Tatei, K.; Izumi, T.
Keystone Symposia, Eicosanoid in Inflammation and Chronic Diseases, Jan. 2006

ヒトGタンパク質共役受容体MrgX2のリガンド探索　　　　　　　　　　　　　　　
Dec. 2005

ヒト表皮角化細胞においてリノール酸酸化物が及ぼす影響　　　　　　　　　　　　　　　
Dec. 2005

Analysis of the mechanism of dimerization/ oligomerization of leukotriene B4 Receptors　　　　　　　　　　　　　　　
Dec. 2005

ショウジョウバエ・ホスホリパーゼD遺伝子の強制発現による解析　　　　　　　　　　　　　　　
Dec. 2005

An UV-inducible G protein-coupled receptor, G2A, functions as a receptor for oxidized free fatty acid such as 9-hydroxyoctadecadienoic acid.　　　　　　　　　　　　　　　
H. Obinata; T. Hattori; A. Ogawa; K. Tatei; T. Izumi
21st century COE Program, The second international symposium on biomedical research using accelerator technology., Nov. 2005

ケラチノサイトにおけるリノール酸酸化物の影響　　　　　　　　　　　　　　　
Nov. 2005

Effects of heavy-ion irradiation on the early embryo of Drosophila melanogaster　　　　　　　　　　　　　　　
TATEI KAZUAKI; OBINATA HIDERU; NONAKA TETUO; NAKANO TAKASHI; IZUMI TAKASHI
21st century COE Program, The second international symposium on biomedical research using accelerator technology., Nov. 2005

Femtogram Mass Biosensor Using a Self-sensing Cantilever for Allergy Check　　　　　　　　　　　　　　　
Hayato Sone; Ayumi Ikeuchi; Takashi Izumi; Haruki Okano; Sumio Hosaka
The 13th International Conference on Scanning Tunneling Microscopy/Spectroscopy and Related Techniques, Jul. 2005

表面プラズモン共鳴法（ＳＰＲ）を用いたＧタンパク質共役型受容体のシグナル解析　　　　　　　　　　　　　　　
16 Jun. 2005

Bioluminescence Resonance Energy Transfer（BRET)を用いたロイコトリエンB4受容体二量体化の解析　　　　　　　　　　　　　　　
16 Jun. 2005

多機能酵素としての低分子型リゾホスホリパーゼとその質量分析への応用　　　　　　　　　　　　　　　
Jun. 2005

Detection of the dimerization of the leukotriene B4 receotor using BRET　　　　　　　　　　　　　　　
Jun. 2005

カンチレバー型フェムトグラムバイオセンサを用いた抗体抗原反応測定　　　　　　　　　　　　　　　
Apr. 2005

Femtogram mass biosensor using a self-sensing cantilever for an allergy checker　　　　　　　　　　　　　　　
Sumio Hosaka; Takafumi Chiyoma; Ayumi Ikeuchi; Haruki Okano; Hayato Sone; Takashi Izumi
2nd International Conference on Advanced Materials and Nanotechnology, Feb. 2005

Femtogram mass biosensor using a piezoresistive cantilever in water　　　　　　　　　　　　　　　
Hayato Sone; Ayumi Ikeuchi; Takashi Izumi; Haruki Okano; Sumio Hosaka
The 12th International Colloquium on Scanning Probe Microscopy, Dec. 2004

Bioluminescence Resonance Energy Transfer（BRET)によるロイコトリエンB4受容体二量体化の検出　　　　　　　　　　　　　　　
Dec. 2004

ショウジョウバエ・ホスホリパーゼD遺伝子の同定と強制発現による解析　　　　　　　　　　　　　　　
Dec. 2004

Actin assembly is a crucial factor for PAF-induced superoxide anion generation from adherent human eosinophils　　　　　　　　　　　　　　　
Suzuki, M.; Kato, M.; Hanaka, H.; Izumi, T.; Morikawa, A.
2nd International Conference on Phospholopase A2 and 8th International Congress on Platelet-Activating Factor and Lipid Mediators, Oct. 2004

Transcriptional regulation for CTP:phosphocholine cytidylyltransferase alfa　　　　　　　　　　　　　　　
Sugimoto, H.; Okamura, K.; Sugimoto, S.; Vance, D. E.; Izumi, T.
2nd International conference on phospholipase A2 and 8th international congress on Platelet-activating factor and related lipid mediators, Oct. 2004

Functional analysis of Phospholipase D gene in Drosophila　　　　　　　　　　　　　　　
Izumi, T.; Tatei, K.
2nd International conference on phospholipase A2 and 8th international congress on Platelet-activating factor and related lipid mediators, Oct. 2004

Overexpression of the Phospholipase D gene affects the external sensory organ cell fate in Drosophila　　　　　　　　　　　　　　　
Tatei, K.; Izumi, T.
2nd International Conference on Phospholopase A2 and 8th International Congress on Platelet-Activating Factor and Lipid Mediators, Oct. 2004

カンチレバー共振型ピコグラムセンサー　　　　　　　　　　　　　　　
Sep. 2004

Femtogram mass sensor using a piezo resistive cantilever for bioscience　　　　　　　　　　　　　　　
Hayato Sone; Yoshinori Fujinuma; Takashi Hieida; Takafumi Chiyoma; Haruki Okano; Sumio Hosaka
Intern. Conf. Micro and Nano Engineering 2004, Aug. 2004

CTP : phosphocholine cytidylyltransfease α 転写のEts-1およびSp1による協調的促進と Net による抑制
18 Jun. 2004

Detection of the Leukotriene B4 Receptor Dimerizarion/ Oligomerization in living cells by Bioluminescence Resonance Energy Transfer (BRET).　　　　　　　　　　　　　　　
27 May 2004

5-リポシキゲナーゼの細胞内局在と活性制御　　　　　　　　　　　　　　　
Mar. 2004

Regulation of the transcription for CTP:phosphocholione cytidylyltransferase a: roles of Sp1 as a co-activator with Ets-1, and Net as an important repressor　　　　　　　　　　　　　　　
Dec. 2003

Characterization of kainite-inducible dentate gyrus-specific cytosolic phospholipase A2 (KIDSCPLA2)　　　　　　　　　　　　　　　
Kishimoto, K.; Nakadate, K.; Shirasawa, T.; Izumi, T.; Uozumi, N.; Watanabe, Y.; Shimizu, T.
33rd Society for Neuroscience annual meeting, Nov. 2003

Overexpression of the Phospholipase D gene in Drosophila.　　　　　　　　　　　　　　　
Tatei, K.; Izumi, T.
First COE International Symposium, Gunma University, "Molecular Mechanisms of Vesicle Trafficking and Membrane Fusion", Nov. 2003

Identification of Ets-1 as an Important Transcriptional Activator of CTP:Phosphocholine Cytidylyltransferase a in COS-7 Cells and Co-Activation with Transcriptional Enhancer Factor-4　　　　　　　　　　　　　　　
Oct. 2003

The mechanism of intracellular localization of LTA4H　　　　　　　　　　　　　　　
Oct. 2003

LTA4水解酵素の細胞内局在とそのメカニズムについて　　　　　　　　　　　　　　　
Jun. 2003

5-oxo-ETE受容体の活性化が引き起こすケモタキシスと細胞内セカンドメッセンジャー濃度変化の測定　　　　　　　　　　　　　　　
Jun. 2003

Glucocorticoids Up-regulate Leukotriene B4 Receptor-1 Expression in HL-60 Cells and Human Neutrophils　　　　　　　　　　　　　　　
Obinata, H.; Yokomizo, T.; Shimizu, T.; Izumi, T.
Keystone Symposia, Eicosanoid Lipid Mediators: From Molecular Discovery to Clinical Application, Mar. 2003

Molecular Cloning and Regulation of Two Leukotriene B4 Receptor　　　　　　　　　　　　　　　
Izumi, T.; Obinata, H.; Yokomizo, T.; Shimizu, T.
Keystone Symposia, Eicosanoid Lipid Mediators: From Molecular Discovery to Clinical Application, Mar. 2003

ショウジョウバエホスホリパーゼD(dPld)遺伝子の強制発現による機能解析　　　　　　　　　　　　　　　
Nov. 2002

脳におけるアラキドン酸含有LPAの2-アラキドノイルグリセロールへの酵素的変換　　　　　　　　　　　　　　　
Nov. 2002

ロイコトリエンB4受容体-1のグルココルチコイドによる発現上昇　　　　　　　　　　　　　　　
Oct. 2002

ショウジョウバエホスホリパーゼD遺伝子の強制発現による機能解析　　　　　　　　　　　　　　　
Oct. 2002

生理活性脂質LPAの分析法に関する研究　　　　　　　　　　　　　　　
Sep. 2002

生理活性脂質の基礎と臨床　　　　　　　　　　　　　　　
Sep. 2002, [Invited]

5-リポキシゲナーゼの細胞内局在と活性制御　　　　　　　　　　　　　　　
30 Aug. 2002

キイロショウジョウバエのホスホリパーゼＤ遺伝子の単離と解析　　　　　　　　　　　　　　　
Jun. 2002

ロイコトリエンB4受容体-1のグルココルチコイドによる発現および機能の亢進　　　　　　　　　　　　　　　
Jun. 2002

Current topics on leukotriene research　　　　　　　　　　　　　　　
Izumi T.
The 12th Congress of Interasma Japan/ North Asia, 06 Mar. 2002

脳の脂質とオーファン受容体　　　　　　　　　　　　　　　
Mar. 2002

ロイコトリエンB4 と病態　　　　　　　　　　　　　　　
Mar. 2002

Receptor-dependent Metabolism of PAF by Macrophage　　　　　　　　　　　　　　　
2002

脳神経系特異的な新規 G タンパク質共役型受容体の遺伝子クローニング　　　　　　　　　　　　　　　
Dec. 2001

5-リポキシゲナーゼの細胞内局在と移行　　　　　　　　　　　　　　　
25 Oct. 2001

5-リポキシゲナーゼの細胞内局在と移行　　　　　　　　　　　　　　　
Oct. 2001

マウスマクロファージにおける受容体依存的なPAFの分解　　　　　　　　　　　　　　　
Oct. 2001

Characterization of lysophospholipase D activity in rat brain　　　　　　　　　　　　　　　
Sugimoto, H.; Iijima, T.; Nakane, S.; Izumi, T.
Seventh International Congress on Platelet-Activating Factor and Lipid Mediators, Sep. 2001

Characterization of two cysteinyl leukotriene receptors (CysLT1 and CysLT2) from mouse　　　　　　　　　　　　　　　
Ogasawara, H.; Ishii, S.; Yokomizo, T.; Shimizu, T.; Izumi, T.
Seventh International Congress on Platelet-Activating Factor and Lipid Mediators, Sep. 2001

Glucocorticoid up-regulates leukotriene B4 receptor expression in HL-60 cells differntiation into neutrophils　　　　　　　　　　　　　　　
Obinata, H.; Yokomizo, T.; Shimizu, T.; Izumi, T.
Seventh International Congress on Platelet-Activating Factor and Lipid Mediators, Sep. 2001

Translocation and localization of 5-lipoxygenase　　　　　　　　　　　　　　　
Hanaka, H.; Shimizu, T.; Izumi, T.
Seventh International Congress on Platelet-Activating Factor and Lipid Mediators, Sep. 2001

Receptor-Dependent Metabolism of PAF by Macrophage　　　　　　　　　　　　　　　
Sep. 2001

5-リポキシゲナーゼの細胞内局在と移行　　　　　　　　　　　　　　　
03 Jul. 2001

ロイコトリエンB4受容体のグルココルチコイドによる発現制御
Jun. 2001

マウス腹腔マクロファージにおける受容体依存的なPAFの分解
Jun. 2001

TRANSLOCATION AND LOCALIZATION OF 5-LIPOXYGENASE　　　　　　　　　　　　　　　
Apr. 2001

生理活性脂質と気管支喘息　　　　　　　　　　　　　　　
2001, [Invited]

ロイコトリエン B4 受容体発現に及ぼすグルココルチコイドの影響　　　　　　　　　　　　　　　
Dec. 2000

ロイコトリエン B4 第二受容体 BLT2 のクローニングと解析　　　　　　　　　　　　　　　
Dec. 2000

EGFP との融合タンパク質を用いた 5 －リポキシゲナーゼの細胞内局在と膜移行の解析　　　　　　　　　　　　　　　
Dec. 2000

Translocaion and localization of 5-lipoxygenase.　　　　　　　　　　　　　　　
H. Hanaka; T. Shimizu; T. Izumi
The 3rd International Conference on Oxygenases, 26 Nov. 2000

Characterization of two forms of leukotriene B4 receptors.　　　　　　　　　　　　　　　
T. Shimizu; T. Yokomizo; T. Izumi
The 3rd International Conference on Oxygenases, 26 Nov. 2000

第二のロイコトリエンB4受容体BLT2のクローニングと解析　　　　　　　　　　　　　　　
Oct. 2000

ロイコトリエン受容体　　　　　　　　　　　　　　　
Oct. 2000

ロイコトリエンの産生制御と受容体　　　　　　　　　　　　　　　
Oct. 2000

NLS- and NES-dependent mechanisms determine the localization of 5-lipoxygenase　　　　　　　　　　　　　　　
Hanaka, H.; Shimizu, T.; Izumi, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Structures and Functions of Leukotriene B4 Receptors　　　　　　　　　　　　　　　
T. Yokomizo; E. Noiri; K. Kato; K. Masuda; A. Toda; N. Ito; T. Izumi; T. Shimizu
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Leukotriene B4 Receptors: from clones to clinics　　　　　　　　　　　　　　　
T. Shimizu; T. Izumi; T. Yokomizo
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Cloning and characterization of rat leukotriene B4 receptor　　　　　　　　　　　　　　　
Toda, A.; Yokomizo, T.; Masuda, K.; Nakao, A.; Izumi, T.; Shimizu, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Cloning and expression of the mouse leukotriene D4 receptor　　　　　　　　　　　　　　　
Ogasawara, H.; Izumi, T.; Shimizu, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Transcriptional mechanism of human leukotriene B4 receptor　　　　　　　　　　　　　　　
Kato, K.; Yokomizo, T.; Izumi, T.; Shimizu, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Critical roles of PAF receptor and cPLA2 in acute lung injury　　　　　　　　　　　　　　　
Ishii, S.; Uozumi, N.; Kume; K; Izumi, T.; Shimizu, T.; Nagase, T.; Ouchi, Y.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Intracellular localization of LTA4 Hydrolase　　　　　　　　　　　　　　　
Ide, Y.; Izumi, T.; Shimizu, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Leukotriene D4 activates microglia through the CysLT1 receptor　　　　　　　　　　　　　　　
Hirabayashi, T.; Ogasawara, H.; Izumi, T.; Shimizu, T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

Localization and translocation of enzymes involved in leukotriene biosynthesis　　　　　　　　　　　　　　　
Izumi T.; Hanaka H.; Ide Y.; Hirabayashi T.; Shimizu T.
11th International Conference on Advances in Prostaglandin and Leukotriene Research, Jun. 2000

マウスのロイコトリエンD4受容体のクローニングと発現　　　　　　　　　　　　　　　
Jun. 2000

脂質メディエーターと生体制御-ロイコトリエン受容体を中心に　　　　　　　　　　　　　　　
Jun. 2000

ヒトロイコトリエンB4受容体遺伝子の転写機構の解析　　　　　　　　　　　　　　　
Dec. 1999

ロイコトリエンの産生制御と受容体　　　　　　　　　　　　　　　
Nov. 1999

アラキドン酸カスケード　　　　　　　　　　　　　　　
Oct. 1999

Localization and translocation of enzymes involved in the synthesis of leukotrienes.　　　　　　　　　　　　　　　
Izumi, T.; Hanaka, H.; Ide, Y.; Hirabayashi, T.; Shimizu, T.
6th International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation and Related Diseases, Sep. 1999

Leukotriene B4 receptor: Structure, transcriptional regulation, and intracellular signaling.　　　　　　　　　　　　　　　
Yokomizo, T.; Kato, K.; Masuda, K.; Toda, A.; Ito, N.; Izumi, T.; Shimizu, T.
6th International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation and Related Diseases, Sep. 1999

Rapid activation of HepG2 cells by 17beta-estradiol　　　　　　　　　　　　　　　
Obinata, H.; Izumi, T.; Shimizu, T.
FASEB Summer Reseach Conference, 08 Aug. 1999

ロイコトリエンB4受容体：細胞内情報伝達、転写調節機構　　　　　　　　　　　　　　　
Jul. 1999

モルモットロイコトリエンB4受容体cDNAのクローニング及び機能解析　　　　　　　　　　　　　　　
Jun. 1999

EGFPとの融合タンパク質を用いた5-リポキシゲナーゼの細胞内局在と膜移行の解析　　　　　　　　　　　　　　　
花香 博美; 和泉 孝志; 清水 孝雄
Jun. 1999

脂質受容体　　　　　　　　　　　　　　　
Apr. 1999

アラキドン酸カスケードの新展開　　　　　　　　　　　　　　　
Apr. 1999

cDNA cloning and characterization of guinea-pig leukotriene B4 receptor　　　　　　　　　　　　　　　
Masuda, K.; Yokomizo, T.; Izumi, T.; Shimizu, T.
Keystone Symposium, Mar. 1999

Cellular localization and teranslocation of 5-lipoxygenase　　　　　　　　　　　　　　　
Hanaka, H.; Izumi, T.; Shimizu, T.
Keystone Symposium, Mar. 1999

メディエーター最前線 ロイコトリエン　　　　　　　　　　　　　　　
Mar. 1999

ロイコトリエンB4受容体の構造と細胞内情報伝達　　　　　　　　　　　　　　　
Oct. 1998

Regulation of Expression of PAF Receptor on Eosinophils in Allergic Inflammation　　　　　　　　　　　　　　　
S. Kishimoto; T. Sugiura; T. Izumi; T. Shimizu; H. Sagara; T. Fukuda; S. Makino; K. Waku
6th International Congress on Platelet-activating Factor and Related Lipid Mediators, Oct. 1998

Agonist-Induced Sequestration and Recycling of PAF Receptor　　　　　　　　　　　　　　　
E. Saito; I. Ishii; T. Izumi; T. Shimizu
6th International Congress on Platelet-activating Factor and Related Lipid Mediators, Oct. 1998

Properties of leukotriene B4 receptor　　　　　　　　　　　　　　　
Izumi T.; Yokomizo T.; Igarashi T.; Masuda K.; Kato K.; Toda A.; Shimizu T.
6th International Congress on Platelet-activating Factor and Related Lipid Mediators, Oct. 1998

ロイコトリエンとアレルギー疾患　　　　　　　　　　　　　　　
Apr. 1998

Molecular cloning of leukotriene B4 receptor　　　　　　　　　　　　　　　
Yokomizo, T.; Izumi, T.; Shimizu, T.
Keystone Symposia on Molecular and Cellular Biology, 23 Mar. 1998

ロイコトリエンB4受容体のクローニングと性質　　　　　　　　　　　　　　　
Nov. 1997

cDNA cloning of LTB4 receptor.　　　　　　　　　　　　　　　
Izumi, T.; Yokomizo, T.; Shimizu, T.
5th International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation and Related Diseases, Sep. 1997

cDNA cloning and functional expression of a human leukotriene B4 receptor.　　　　　　　　　　　　　　　
Yokomizo, T.; Izumi, T.; Chan, K.; Takuwa, Y.; Shimizu, T.
Sep. 1997

Molecular cloning of leukotriene B4 receptor.　　　　　　　　　　　　　　　
Yokomizo, T.; Izumi, T.; Shimizu, T.
17th International Congress of Biochemistry and Molecular Biology, Aug. 1997

PAF受容体 -遺伝子から臨床まで-　　　　　　　　　　　　　　　
Apr. 1997

Pathophysiological roles of PAF in vivo.　　　　　　　　　　　　　　　
Ishii, S.; Kume, K.; Izumi, T.; Shimizu, T.; Nagase, T.; Fukuchi, Y.; Kuwaki, T.; Kumada, M.; Ikuta, K.; Tashiro, H.; Miyazaki, J.-I.; Noma, M.
10th International Conference on Prostaglandins and Related Compounds, Oct. 1996

Exchanges of polar amino acids to alanines of platelet-activating factor receptor generate constitutive active and inactive mutants with altered affinities and altered cellular responses to their ligands.　　　　　　　　　　　　　　　
Ishii, I.; Izumi, T.; Ui, M.; Shimizu, T.
10th International Conference on Prostaglandins and Related Compounds, Sep. 1996

Platelet-activating factor receptor - Signal transduction and gene regulation.　　　　　　　　　　　　　　　
Shimizu, T.; Izumi, T.
10th International Conference on Prostaglandins and Related Compounds, Sep. 1996

cDNA Cloning of Leukotriene B4 12-Hydroxydehydrogenase　　　　　　　　　　　　　　　
Izumi T.; Yokomizo T.; Shimizu T.
10th International Conference on Prostaglandins and Related Compounds, Sep. 1996

Exchanges of polar amino acids to alanine generate platelet-activating factor receptor mutants with altered affinities to their ligand.　　　　　　　　　　　　　　　
Ishii, I.; Izumi, T.; Ui, M.; Shimizu, T.
American Society of Investigative Pathology, American Association of Immunologists, Jun. 1996

アレルギー性気管支喘息における好酸球ＰＡＦ受容体の発現　　　　　　　　　　　　　　　
Feb. 1996

ＰＡＦトランスジェニックマウスの解析　　　　　　　　　　　　　　　
Feb. 1996

血小板活性化因子受容体のリガンド結合部位の解析　　　　　　　　　　　　　　　
Feb. 1996

PAFと気管支喘息　　　　　　　　　　　　　　　
1996

血小板活性化因子受容体およびロイコトリエン受容体について　　　　　　　　　　　　　　　
1996, [Invited]

Cloning and characterization of a platelet-activating factor receptor gene.　　　　　　　　　　　　　　　
Ishii, S.; Nakamura, M.; Waga, I.; Kume, K.; Izumi, T.; Shimizu, T.
4th International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, Oct. 1995

Expression and mutagenetic study of leikotriene B4 12-hydroxydehydrogenase.　　　　　　　　　　　　　　　
Yokomizo, T.; Kume, K.; Izumi, T.; Shimizu, T.
4th International Conference on Eicosanoids & Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, Oct. 1995

ブタ白血球ロイコトリエンB4受容体の部分精製と再構成　　　　　　　　　　　　　　　
Apr. 1995

PAF受容体を介する細胞内応答　　　　　　　　　　　　　　　
Mar. 1995

Regulation and signal transduction of PAF receptor.　　　　　　　　　　　　　　　
Izumi, T.
FASEB Summer Research Conferences on Cytokine and Lipid Mediator Networks, 1995, [Invited]

Leukotriene B₄(LTB₄)の新しい不活化経路 (I) LTB₄ 12‐Hydroxydehydrogenaseの精製と性質
Jun. 1994

Leukotriene B₄(LTB₄)の新しい不活化経路 (II) 代謝物の構造決定
Jun. 1994

Regulation and signal transduction of PAF receptor.　　　　　　　　　　　　　　　
Izumi, T.; Honda, Z.; Mutoh, H.; Kume, K.; Shimizu, T.
9th International Conference on Prostaglandins and Related Compounds, Jun. 1994, [Invited]

Purification and characterization of leukotriene B4 (LTB4) 12-hydroxydehydrogenase.　　　　　　　　　　　　　　　
Yokomizo, T.; Uozumi, N.; Izumi, T.; Shimizu, T.
9th International Conference on Prostaglandins and Related Compounds, Jun. 1994

エイコサノイド・PAF受容体と情報伝達　　　　　　　　　　　　　　　
Apr. 1994

LTB4とPAFについての最近の研究　　　　　　　　　　　　　　　
1994

Biosynthesis and Metabolism of Leukotriene (LT)B4.　　　　　　　　　　　　　　　
Yokomizo, T.; Minami, M.; Izumi, T.; Shimizu, T.
International Symposium on Molecular Biology of the Arachidonate Cascade, Dec. 1993

Characterization of the PAF receptor.　　　　　　　　　　　　　　　
Izumi, T.; Honda, Z.; Mutoh, H.; Shimizu, T.
International Symposium on Molecular Biology of the Arachidonate Cascade, Dec. 1993

ＰＡＦ受容体を介する情報伝達の解析　　　　　　　　　　　　　　　
Dec. 1993

血小板活性化因子(PAF)による生体制御 -PAF受容体のクローニングと情報伝達-　　　　　　　　　　　　　　　
Nov. 1993

ＰＡＦ受容体を介する情報伝達の解析　　　　　　　　　　　　　　　
Oct. 1993

Leukotriene A4 hydrolase, a bifunctional enzyme　　　　　　　　　　　　　　　
Izumi T.; Minami M.; Ohishi N.; Bito H.; Shimizu T.
The 8th International Conference on Prostaglandins and Related Compounds, Jul. 1992

血小板活性化因子と情報伝達　　　　　　　　　　　　　　　
Jun. 1992

Leukotriene A4 hydrolase, a bifunctional enzyme.　　　　　　　　　　　　　　　
Izumi, T.; Minami, M.; Bito, H.; Shimizu, T.
First IUBMB Conference, Biochemistry of Dieseases, Jun. 1992

ＰＡＦ受容体と情報伝達　　　　　　　　　　　　　　　
1992, [Invited]

脂質性メディエーターによる細胞制御　　　　　　　　　　　　　　　
1992

Biosynthesis of leukotriene C4.　　　　　　　　　　　　　　　
Izumi, T.; Shimizu, T.; Tsuchida, S.; Sato, K.
First Biennial International Conference on Amino Acid Research, Aug. 1991

ＰＡＦ受容体について　　　　　　　　　　　　　　　
1991

アラキドン酸のリポキシゲナーゼファミリーについて　　　　　　　　　　　　　　　
1991

ロイコトリエン(LT)A4の代謝 LTA4水解酵素とLTC4合成酵素の精製及び性質　　　　　　　　　　　　　　　
Jul. 1987
ヒト肺よりLTA4水解酵素を,モルモット肺よりLTC4合成酵素の精製を行い,その性質につき検討した.ヒト肺のLTA4水解酵素は,均一な蛋白に精製され,分子学的性質において,ヒト白血球の酵素と類似し,基質であるLTA4の他,基質とならないLTA4 isomerによっても不活性化された.モルモット肺のLTC4合成酵素は,ミクロソーム画分に回収され,塩及びdetergentにより可溶化された.本酵素活性はゲル濾過カラム等にてグルタチオン-S-トランスフェラーゼ活性と分離され,さらにイオン交換,ハイドロキシアパタイトカラムにて部分精製された

モルモット全身臓器におけるLTA4水解(LTB4合成)酵素活性およびLTC4合成酵素活性の分布　　　　　　　　　　　　　　　
Jun. 1986
LTA4を基質としたときのLTA4水解(LTB4合成)酵素活性およびLTC4合成酵素活性のモルモット主要臓器における分布を測定し,血球細胞に比べて種々の臓器に高いLT合成活性のあることを見出した.この知見は炎症反応における各臓器の果たす役割や,臓器の機能に関係したLTの作用を知る上で重要である

マウスマスト細胞のカルシウム依存性5-リポキシゲナーゼの精製と性質　　　　　　　　　　　　　　　
Jun. 1986

マスフラグメントメトリーによるロイコトリエンB4及びプロスタグランジンの微量定量　　　　　　　　　　　　　　　
Jul. 1985

Feb. 2024 - Present
Sigma Xi　　　　　　　　　　　　　　　

Stress-relieving effects of acupuncture using salivary components as an indicator
Grant-in-Aid for Challenging Research (Exploratory)
Kochi University
Jun. 2022 - Mar. 2025

Development of ultrahigh-sensitive silicon nanowire biosensor for simultaneous detection of various biomolecules in a single drop of body fluid
Grant-in-Aid for Challenging Research (Exploratory)
Gunma University
Jul. 2020 - Mar. 2023

環境暴露とアレルギー及び呼吸器疾患発症との関連を解明するためのエクスポソーム解析
Apr. 2018 - Mar. 2021

小児の化学物質複合曝露メカニズムの解明と質量分析を用いた革新的基盤技術の開発
Apr. 2018 - Mar. 2020

小児特有の化学物質複合曝露メカニズム解明とリスク管理モデルの提案　　　　　　　　　　　　　　　
Apr. 2017 - Mar. 2020

Studies on G protein-coupled receptor controlling cell cycle and stemness
Grant-in-Aid for Scientific Research (B)
Gunma University
Apr. 2012 - Mar. 2017
G2A is a G protein-coupled receptor that uses oxidized free fatty acids as ligands. The addition of oxidized free fatty acids such as 9-HODE to the cultured cells resulted in cell cycle arrest. G2A induction was also observed by stress stimulation such as glucose starvation and hypoxia. It became clear that G2A induction is deeply involved in changes in intracellular pH and glutathione concentration. When glioma cells expressing G2A were transplanted into immunodeficient mice, tumor-formation and metastasis to the organs was observed. Immunohistochemical analysis of metastatic lesion revealed that epithelial mesenchymal transition occurred.
Thus, G2A, a stress-inducible GPCR, stops the cell cycle in response to oxidized free fatty acids. On the other hand, G2A increases stemness in tumor cells resulting in epithelial mesenchymal transition and forms metastatic foci.

Analysis of receptor signaling in living cells by surface plasmon resonance
Grant-in-Aid for Challenging Exploratory Research
Gunma University
Apr. 2011 - Mar. 2014
Various methods have been used for the analysis of intracellular signaling from cell membrane receptors. However, there are problems in the reproducibility and operability. Previously, we have succeeded in measuring the SPR (surface plasmon resonance) through GPCR (G-protein coupled receptor) in living cells. In this study, we elucidated the part of the intracellular signaling pathways of GPCR signal using various inhibitors. Furthermore, we succeeded in measuring the SPR though RTKs (tyrosine kinase receptors), another group of membrane receptors. We also observed SPR signal through GPCR using a small portable SPR device.

生体調節シグナルの統合的研究　　　　　　　　　　　　　　　
Apr. 2007 - Mar. 2012

Functional analysis and its application of a G-protein coupled receptor, whose ligands are oxidized free fatty acids
Grant-in-Aid for Scientific Research (B)
Gunma University
Apr. 2006 - Mar. 2008
A purpose of this study was to clarify the physiological and pathological functions of a G-protein coupled receptor called G2A, and was to investigate the basic aspects of G2A signaling to establish new diagnosis / therapy. G2A is a receptor which ligands are oxidative flee fatty acids such as 9-HODE and 11-HETE derived from linoleic acid or arachidonic acid, respectively. It was reported that G2Awas induced by various DNA damage stimulation and its stimulation causes arrest of cell cycle. However, the physiological function of G2Awas unidentified.
As skin is routinely and pathologically exposed to many oxidative stresses such as UV radiation, chemical agents, and inflammation that might induce both G2A expression and production of G2A ligands, we examined G2A fraction in human keratinocytes. G2A was expressed in human epidermis and normal human epidermal keratinocytes (NHEK). 9-HODE evoked intracellular calcium mobilization, and secretion of cytokines including IL-6, IL-8, and GM-CSF in NHEK cells. These responses became prominent in HaCaT cells, an immortalized human keratinocyte cell line, by overexpression of G2A. 9-HODE further inhibited proliferation of NHEK cells by suppressing DNA synthesis and arresting the cell cycle in the G0/1-phase, and down-regulation of G2A expression by small interfering RNA caused suppression of 9-HODE-induced effects on proliferation of NHEK cells 13-HODE, much weaker ligand of G2A, did not show any effects on either cytokine secretion and proliferation in NHEK cells. On the other hand, UVB and hydrogen peroxide induced G2A expression and caused oxidation of linoleate to produce 9-HODE in HaCaT cells.
These results suggest that 9-HODE-G2A signaling plays proinflammatory roles in skin under oxidative conditions.

生体情報の受容伝達と機能発現　　　　　　　　　　　　　　　
Apr. 2002 - Mar. 2007

新規生理活性脂質の生体機能
Apr. 2001 - Mar. 2006

Roles and regulation of lipid mediators
Grant-in-Aid for Scientific Research (A)
The University of Tokyo
Apr. 2000 - Mar. 2003
Upon cell stimulation, various types of phospholipases are activated, and a class of lipid mediators are produced. They play important role in self-defense and maintenance of homeostasis. In the last three years, we have carried out biochemical and genetic experiments, and obtained results as follows. The results have been published in almost 50 original articles and 6 reviews or book chapters.
1. Studies on cytosolic phospholipase A2 and related molecules.
cPLA2α-null mice showed impairment in synaptic plasticity and reproduction. The enzyme also plays important roles in inflammatory bone resorption, autoimmune arthritis, acute respiratory distress syndrome (ARDS) and bleomycin-induce pulmonary fibrosis. Thus, a selective cPLA2a inhibitor would be beneficial for these disorders. In addition to a subtype of cPLA2, we also found β, and γ subtypes of the enzyme. While β-subtype has Ca-sensitive C2 domain, g-subtype is activated by reactive oxygen species. We also found a novel truncated isoforms of cPLA2, specifically expressed in dentate gyrus by stimulation.
2. Characterization of leukotriene receptors.
We identified 4 different types of leukotriene receptors (BLT1, BLT2, CysLT1, and CysLT2). They are differently expressed in cells and tissues, by cognate promoter activations. We elucidated detailed signal transduction of these receptors, and established cell, either overexpressing or lacking these receptors. More systematic phenotype analyzes will be done by gene targeting of the mice.
3. Characterization of other types of receptors.
We analyzed PAF (platelet-activating factor) receptor, and further found that PAF is deeply involved in inflammatory responses, capacitation of sperm, and neuronal migration in cerebellar granular cells. We also identified the fate of PAF (its breakdown to lysophospholipids, and reacylation to 1-alky1-phospholipids). By SNP analyzes, we found a novel mutation in the third intracellular domain in asthmatic family.

ノックアウトマウスを使ったcPLA2の機能解析と新規PLA2の検索
Apr. 1998 - Mar. 2002

Analysis of mechanisms of neutrophil accumulation in the lung ; research utilizing LTB_4 receptor-expressed CHO cells.
Grant-in-Aid for Scientific Research (C)
University of Tokyo
Apr. 1999 - Mar. 2001
1. Determination of optimal condition of labeling LTB_4 receptor-expressed CHO cells with fluorescent probes.
CHO cells could be adequately labeled for visualization with fluorescent microscopy with 4 micro molar CMFDA or 2 micro molar CMTMR.LTB_4 receptor-expressed CHO cells labeled with this conditions showed the same chemotaxis against LTB_4 as unlabeled cells in chemotaxis assay using chemotaxis chamber.
2. Evaluation of neutrophil accumulation in mice lung with intratracheal administration of various agents and its reproducibility.
As models of neutrophil accumulation in mice lung, we administered LTB_4, bleomycin, and silica intratracheally. After 4 hours of LTB_4 administration, neutrophilia over 10% in bronchoalveolar lavage fluid (BALF) cells were observed in only about 20% of examined mice, and after 24 hours of bleomycin administration, neutrophilia over 50% were observed in only about 50% of examined mice, suggesting that the reproducibility of neutrophil accumulation in mice lung by these methods was not adequate. In case of administration of silica, when silica was recovered in BALF, there were consistent marked neutrophilia (40-80%) in BALF cells 24 hours after silica administration, but, when silica was not recovered in BALF, neutrophilia were not observed. This findings indicated that expectoration and inaccessibility to lung parenchyme of intratracheally administered agents were the main cause of inadequate reproducibility of the procedures. Silica was a suitable agent for intratracheal administration in that its deposition into lung parenchyme could be verified by BALF analysis.
3. in vivo Chemotaxis assay in mice model of intratracheal administration of silica using CMFDA-labeled LTB_4 receptor-expressed CHO cells.
CMFDA-labeled LTB_4 receptor-expressed CHO cells and CMTMR-labeled control CHO cells were injected to mice intravenously via tail veins after 20 hours of intratracheal administration of silica. However, in BALF obtained 4 hours later, neither CMTMR nor CMFDA-labeled cells could not be recovered. Because there was a possibility that LTB_4 receptor-expressed CHO cells was not present freely in alveolar space but attached to pulmonary capillary endothelium or alveolar epithelium, we made histological specimens of lung tissues and examined fluorescent probe-labeled cells, but, at present, we could not obtain any definite results.

INVOLVEMENT OF LIPID MEDIATORS IN INFLAMMATORY DISORDERS
Grant-in-Aid for Scientific Research (C)
Apr. 1999 - Mar. 2001
Lipid mediators such as leukotrienes (LTs) and platelet-activating factor (PAF) are major chemical mediators involved in inflammatory and allergic disorders. To clarify the pathological roles of these mediators in inflammatory disorders, this research project was carried out, and following results were obtained.
1. Establishment and analysis of inflammatory animal model
In PAF receptor transgenic mice, PAF receptor deficiency mice, cPLA2 deficiency mice, and wild-type mice, ARDS (adult respiratory distress syndrome) models were made by intratracheal administration of acid or intravenous administration of LPS, and analyzed. The obtained results indicate that PAP and cPLA2-dependent mediators play important roles in these conditions.
PAF receptors are overexpressed in Kupffer cells after experimental hepatectomy in rat.
2. Study on LTB4 receptor
The mechanism of leukocyte-specific expression of LTB4 receptor was revealed by its promoter analysis. Using in vivo chemotaxis of CHO cells expressing LTB4 receptor, the possible roles of LTB4 in pathogenesis of acute renal inflammation were indicated. The second receptor for LTB4 was discovered. The project on establishment of LTB4 deficiency mice is now in progress.
3. Signal transduction through LTD4 receptor
Intracellular signal transduction through LTD4 receptor was analyzed in monocytes and macrophages.
4. Intracellular localization of enzymes involved in production of lipid mediators
The mechanisms of intracellular localization and translocation of enzymes such as cPLA2, 5-lipoxygenase and LTA4 hydrolase were studied.
These results revealed that mechanisms of lipid mediator production and pathological roles of lipid mediators in inflammatory disorders, and may help us develop new therapies and drugs.

FUNCTIONAL REGULATION OF RECEPTORS FOR LIPID MEDIATORS
Grant-in-Aid for Scientific Research (B)
The University of Tokyo
Apr. 1998 - Mar. 2000
Lipid mediators such as leukotrienes (LTs) and platelet-activating factor (PAF) are major chemical mediators involved in inflammatory and allergic disorders. Despite their potent biological activities, little is known about the function and translational regulation of their receptors. To clarify these questions, we carried out following experiments and obtained some results that may contribute better understanding of patho-physiological roles of lipid mediators and development of new therapies and drugs for inflammatory and at11ergic disorders.
1. We analyzed the mechanisms of ligand-induced internalization of PAP receptors.
2. Using PAP receptor knockout mice and cPLA2 knockout mice, the roles of lipid mediators in acute lung injuries occurred by LPS and acid were investigated.
3. Functional coupling of LTB4 receptor with G-proteins in porcine WBC was identified.
4. Analysis of human genome for LTB4 receptor was carried out. The mechanisms for leukocyte specific expression of LTB4 receptor was clarified.
5. cDNA clones for LTB4 receptors of mouse, rat and Guinea pig were identified. And their functions were analyzed.
6. We analyzed the signal transduction mechanisms through LTD4 receptors using human monocytic leukemia THP-1 cells.

MECHANISM OF NEURONAL DEATH AND REGENERATION
Grant-in-Aid for Scientific Research (B)
THE UNIVERSITY OF TOKYO
Apr. 1998 - Mar. 2000
Neuronal survival and death are determined by multiple factors including neuronal activity as well as an influence from surrounding glia. In this project, we focus on various lipid mediators in terms of its ability of interaction between neuron and glia. We found that various lipid mediators are produced by stimuli, and play roles in the network as follows. LPA, produced by various cells, stimulates growth and cell maturation of neuron as well as proliferation of astrocytes. LPA also releases various cytokines and neurotrophis (NGF, BDNF) from astroglia, which activates neuronal cells. PAP is released from neuron by the NMDA stimulation, and acts on microglia. Microglia produces NO, and causes chemotaxis towards neuron, suggesting the role of microglia for repairing neuronal cells. PAF receptor-deficient mice and phospholipase A2 (cPLA2)-deficient mice are established, and their congenic mice are now available by backcrossing. The exact roles of these lipid mediators will be clarified by extensive analyses of their phenotypes.

IN VIVO AND INTRACELLULAR ROLES OF LEUKOTRIENE B4 RECEPTOR.
Grant-in-Aid for Scientific Research (C)
UNIVERSITY OF TOKYO
1999 - 2000
To investigate the biological functions of leukotriene B4 receptor in vivo, we established a line of mice deficient in high-affinity leukotriene B4 receptor (BLT1). Embryonic stem (ES) cells were transfected with a targeting vector for BLT1, and several clones were isolated and confirmed for their homologous recombination by Southern blotting. These ES cells were microinjected to blastcysts, transferred to pseudopregnant female mice, and subsequently chimeric mice were obtained. These chimeric mice are crossed among them, and finally BLT1-deficient mice were born. Now these mice are under backcrossing to examine their phenotype.
CHO cells stably expressing BLT1 showed robust chemotaxis toward LTB4 in vitro, so we tried to examine the chemotactic activities of these cells in vivo. Rat acute ischemic-reperfusion renal injury was initiated by 45 minute-clamping of the renal artery. After 24 hrs, cells were injected into the injured kidney, and were allowed to chemotax for 4 hrs. Only in case of ischemic kidney, not in the sham-operated kdney, CHO cells expressing BLT1 migrated into the peritubal portion of the kidney. Control cells transfected with empty vector did not migrate at all. Prefeeding of the rats with BLT antagonist (Ono 4057) not only inhibited the cells migration, but also reduced the severity of the renal failure, suggesting that LTB4-BLT1 interaction plays important roles in the initiation and the progression of ischemic renal failure.(Noiri, E., et al Proc.Natl.Acad.Sci.U.S.A., 2000)
To clarify the molecular mechanisms of the cell-specific expression of BLT1, we examined the transcriptional regulation of BLT1. BLT1 gene is a small gene within 4 kbps, and its promoter contains no TATA-box, but an Initiator sequence. Serial deletion analyses revealed that Sp-1 at-50 from the initiation site plays a pivotal role in BLT1 transcription. Moreover, the promoter region was totally methylated in BLT1-nonexpressing cells, but not in the expressing cells. In vitro methylation of BLT1 promoter almost completely inhibited its promoter activity.(Kato, K., et al.J.Exp.Med.2000)
On the course of analyzing BLT1 promoter, we found a novel gene with structural similarities with BLT1. The encoded protein was a novel G-protein-coupled receptor, and exhibited a low-affinity, but significant binding to LTB4. This receptor efficiently transduces intracellular signaling toward calcium increase, inhibition of ademylyl cyclase, and chemotaxis by application of LTB4, thus we named it a second LTB4 receptor, BLT2.(Yokomizo, et al.J.Exp.Med.2000, Yokomizo, T.et al.J.Biol.Chem.2001)

血小板活性化因子(PAF)受容体の機能解析と制御
Apr. 1998 - Mar. 1999

Molecular Cloning and Signal Transduction of Leukotriene Receptors
Grant-in-Aid for Scientific Research (C)
The University of Tokyo
Apr. 1997 - Mar. 1999
Leukotrienes are major lipid mediators involved in inflammatory and allergic disorders. Despite their potent biological activities, little is known about the receptors and intracellular signaling pathways.
1.We cloned a cDNA for a LTB_4 receptor (BLT) from HL-60 cells differentiated with retinoic acid using a subtraction strategy. The open reading frame (ORF) of the cDNA encodes a protein of 352 amino acids and is predicted to contain seven membrane-spanning domains. In CHO cells stably expressing BLT, LTB_4 elicited many signal transductions such as increase in intracellular calcium, InsP_3 accumulation, and inhibition of adenylyl cyclase. The CHO cells revealed a marked chemotactic response toward nM order of LTB_4. Calcium increase and InsP_3 accumulation induced by LTB_4 were partially sensitive to pertussis toxin (PTX), while chemotaxis and inhibition of adenylate cyclase were completely sensitive to PTX, indicating that BLT transduces LTB_4 signals through both PTX-sensitive and -insensitive G proteins.
2.We analyzed the signal transduction mechanisms through LTD_4 receptors using human monocytic leukemia THP-1 cells. When these cells were stimulated with LTD_4, intracellular calcium concentration was increased and mitogen-activated protein kinase (MAP kinase) was activated. A chemotactic response of THP-1 cells toward LTD_4 was also observed. We found that LTD_4 has at least two distinct signaling pathways in THP-1 cells, a PTX-insensitive mitogen-activated protein kinase activation through protein kinase Calpha and Raf-1 and a PTX-sensitive chemotactic response. This cellular signaling can explain in part the versatile activities of LTD_4 in macrophages under inflammatory and allergic conditions.

血小板活性化因子(PAF)受容体の機能解析と制御
Apr. 1997 - Mar. 1998

Elucidation of roles of platelet-activating factor receptor by gene targeting.
Grant-in-Aid for Scientific Research (B)
University of Tokyo
Apr. 1996 - Mar. 1998
Platelet-activating factor (PAF) is a potent phospholipid mediator involved in inflammatory and allergic responses. It is also reported that PAF plays major roles in endotoxin-induced lethality, reproduction and brain development. To determine the role of PAF and its receptor in vivo, we generated a PAF receptor-deficient mice, by honologous recombination. Offspring were obtained with a Menderian distribution. They grow up normally, and no developmental changes are observed in any organs examined. The level of blood immunoglobulins and differential cell counts show no abnormality.
When the mice were sensitized and challenged with ovalbumin, the wild type mice showed a marked bronchial constriction, pulmonary edema, hypotension and death. On the other hand, the PAF receptor-deficient mice showed only moderate response to these treatments. Upon challenging with endotoxin, no significant difference was observed in both groups. Thus, we concluded that PAF is deeply involved in anaphylactic responses, while it shows minor role in the endotoxin-induced pathologies and lethality. Considering PAF antagonists show some protective effects on endotoxin-shock, even in PAF receptor knock-out mice, there may exist other targets of PAF receptor antagonists to protect the living system from endotoxin-induced disorders.

PAF受容体トランスジェニックマウスを用いた気管支喘息発症メカニズムの解明
Apr. 1996 - Mar. 1997

血小板活性化因子受容体の構造と機能
Apr. 1996 - Mar. 1997

心筋細胞におけるPAF受容体機能の解析
Apr. 1996 - Mar. 1997

Pathophysiology and Treatment of Endotoxin Shock
Grant-in-Aid for Scientific Research (A)
University of Tokyo
Apr. 1995 - Mar. 1997
Endotoxin causes systemic collapse, disseminated intravascular coagulation and lethality, by infection of gram-negative bacteria. Although many reports the production of cytokines, the precise mechanism of pathophysiological events remained obscure. By cell biology and molecular biology techniques, we obtained following results.
(1) Elucidation of relation between endotoxin and PAF receptor
We established transgenic mice overxpressing PAF receptor. These mice showed severe symptoms of endotoxin shock and death by intravenous administration of small doses of LPS.The lethality was efficiently blocked by the use of PAF receptor antagonists, suggesting that PAF-PAFR is involved in the pathogenesis of endotoxin-induced lethality.
(2) Elucidation of LPS action on macrophages
By incubating of macrophages with bacterial endotoxin, PAFR mRNA was increased several fold after 4h. MAP kinase was also activated, and the activation related to the tyrosine phosphorylation of Raf-1 kinase.

トランスジェニックマウスを用いた心筋細胞におけるPAF受容体の解析
Apr. 1995 - Mar. 1996

血小板活性化因子(PAF)受容体の発現調節
Apr. 1995 - Mar. 1996

炎症細胞における血小板活性化因子(PAF)受容体の発現と作用
Apr. 1994 - Mar. 1995

血小板活性化因子による増殖活性化機構の解析
Apr. 1994 - Mar. 1995

トランスジェニックマウスを用いた心筋細胞におけるPAF受容体の解析
Apr. 1994 - Mar. 1995

STUDIES ON PHYSIOLOGICAL FUNCTION OF PLATELET-ACTIVATING FACTOR
Grant-in-Aid for General Scientific Research (B)
THE UNIVERSITY OF TOKYO
Apr. 1993 - Mar. 1995
Following results were obtained on the physiological role and cellular signaling of platelet-activating factor (PAF).
(1)Cellular signaling via PAF receptor
By using transfected receptor in CHO cells, cellular signaling after PAF application was determined. PAF stimulates phosphoinositide turnover, mitogen-activated protein (MAP) kinase, phospholipase A2, and inhibits adenylate cyclase. From the results of PTX-treatment, PAF receptor appears to couple with two types of G-proteins. Ca-independent activation of MAP kinase was inhibited by wortmannin, suggesting a wortmannin-sensitive molecule such as phosphatidylinositol 3-kinase is present between Gi and MAP kinase.
To determine the molecular mechanisms of signal desensitization by PAF,we prepared CHO cells carrying wild-type, C-terminal deletion mutant, and substitution mutant (Ser, Thr to Ala) receptors. We found that C-terminal cytoplasmic tail is not required for forward signal transduction, but it is essential for signal-shut down. A family of GRK (G-protein receptor kinase) may be involved in the phosphorylation of Ser/Thr residues leading to signal shut down.
(2)Genomic structure of PAF receptor.
Human PAF receptor gene is present as a single copy in haploid on the chromosome 1q. The gene consists of three exons, separated each other > 20kb intron. Two5'-noncoding exons has distinct transcriptional start site, and promoters. These exons are alternatively spliced to a common splice acceptor to yield two different mRNAs. Transcript 1 is present in almost all tissues, and up-regulated by PAF or endotoxin through its NF-kB site. Transcript 2 is expressed in more tissue-specific way, and up-regulated by estrogen, and down-regulated by TGF-b. The physiological and pathological significance of these promoters are currently under investigation.

THE MECHANISM AND BIOSYNTHESIS OF BIOACTIVE LIPIDS
Grant-in-Aid for General Scientific Research (C)
The University of Tokyo
Apr. 1992 - Mar. 1994
1. STUDY FOR LEUKOTRIENE BIOSYNTHESIS AND METABOLISM
A monoclonal antibody to LTC_4 synthase was developed, and using this antibody enzyme purification is now going on.
A mutagenesis study of LTA_4 hydrolase revealed that the active center for LTB_4 producing activity is different from that for aminopeptidase activity.
We showed that LTB_4 is inactivated by LTB_4-12-hydroxydehydrogenase in porcine kidney, and this enzyme was purified to apparent homogeneity.
2. STUDY FOR FUNCTION OF LEUKOTRIENE
Studies for cDNA cloning of leukotriene receptors are going on using frog oocyte expression system or mammalian cell expression system.
3. STUDY FOR PAR RECEPTOR
The presence of functionally active PAF receptor in rat brain have been indicated.
In a human eosinophilic leukemia cell line (EoL-1 cells), PAF receptor were induced during differentiation by sodium butyrate.
Studies on human PAF receptor gene revealed that two different promoter exist to produce two types of mRNA, namely leukocyte type and heart type.
In CHO cells transfected with PAF receptor, PAF activated MAP kinase and MAP kinase kinase.

Screening and Development of New Immnomodulatory Drugs using PAF Receptor Gene as a Probe
Grant-in-Aid for Developmental Scientific Research (B)
The University of Tokyo
Apr. 1991 - Mar. 1993
Platelet-activating factor (PAF) is a potent mediator of inflammation, immunological disorders, and endotoxin shock. We have recently isolated cDNA clones of human and guinea pig PAF receptors. Using these clones as probe, we have obtained following results.
1. Isolation and structure of PAF receptor gene.
PAF receptor gene is consisted of three exons which are separated by >20kb introns. All exons are localized on chromosome 1. By alternative splicing, the leukocyte- and heart-type transcripts are produced. They have differentially regulated by distinct promoters.
2. Elucidation of signal transduction through a cloned PAF receptor.
A stable transfectant cell (CHO) was established carrying a cloned PAF receptor. By the application of PAF, phospholipase C and A_2, and MAP (mitogen-associated protein) are all activated, while adenylate cyclase was inhibited. These cellular events appear to be regulated by different types of G-proteins. Furthermore, we found that endotoxin. LPS, stimulated PAF receptor and yielded a Ca increase in human platelets as well as CHO transfectants. Thus, a part of LPS signaling, especially that related to platelet-aggregation might be due to the direct activation of PAF receptor.
3. Development of Screening System of PAF Receptor Agonists and Antagonists.
PAF receptor was expressed in Xenopus oocytes and a sensitive assay system of PAF receptor agonists/antagonists was made. Furthermore, COS cells/CHO cells carrying an excess amount of PAF receptors were constructed. By using membrane fractions of these cells, the highly specific screening system of PAF receptor modulator (either receptor antagonists or uncoupler of receptor-G-protein) was established. Through screening of naturally-occurring and synthetic compounds, several candidate chemicals have been obtained, which have potentiality for immunomodulatory drugs.

Studies on Leukotriene Biosynthesis and Signal Transduction.
Grant-in-Aid for General Scientific Research (B)
The University of Tokyo
1990 - 1992
PAF and leukotrienes are lipid mediators involved in inflammatory/allergic response as well as various cardiovascular and immunological disorders. We have carried out experiments on biosyntheses and signal transduction of these lipid mediators.
1. Isolation of PAF and leukotriene receptors.
By expression cloning using Xenopus oocyte system, we isolated a cDNA of PAF receptor as the first successful example of receptor cloning of lipid mediators. A human homologue was also obtained. They are composed of 342 amino acid residues with putative seven transmembrane spanning domains. These receptors were expressed in COS cells or CHO cells, and cellular signalings are extensively studied. It was shown that PAF receptor couples to phospholipases, adenylate cyclase, and MAP kinase via different types of G- proteins. Leukotriene receptors (B4, and D4) were solubilized from guinea pig leukocyte and lung membrane, respectively in an active form. They activate phospholipase A_2 and C via Gi proteins. Molecular cloning of these receptors by two strategies (protein purification and expression cloning) is currently ongoing.
2. Leukotriene A_4 hydrolase. a bifunctional enzyme.
Leukotriene B_4, a potent chemotactic factor, is produced by an enzyme termed leukotriene A4 hydrolase. By cDNA cloning and elucidation of the primary structure of the enzyme have shown that the enzyme is highly homologous to various types of metalloproteinases. In fact, it was shown that the enzyme contains a zinc ion, which is essential for both intrinsic aminopeptidase activity and leukotriene A_4 hydrolase. To determine the active site of the enzyme, site-directed mutagenesis experiments were performed. The mutation of His-296, or Glu-315 yielded loss of zinc ion with concomitant disappearance of both enzyme activities. However, by changing Glu-297 to other amino acids. two enzyme activities were clearly separated. The mechanism of incorporation of water molecule as well as the physiological significance of bifunctional role of enzyme remain unclear.

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