Yuko Ogawa
| Faculty of Pharmaceutical Sciences,Department of Pharmaceutical Sciences | Associate Professor |
| Graduate School of Pharmaceutical Sciences,Doctoral Program in Pharmaceutical Sciences | Associate Professor |
Last Updated :2025/10/07
■Researcher basic information
■Career
Career
- Apr. 2014 - Present
Teikyo Heisei University, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Membrane biology, Associate professor - Apr. 2010 - Mar. 2014
Teikyo Heisei University, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Lecturer - Apr. 2009 - Mar. 2010
Hoshi University, Institute of Medicinal Chemistry, Bioorganic Chemistry, Research associate - Apr. 2007 - Mar. 2009
Hoshi University, Institute of Medicinal Chemistry, Laboratory of Biological resources, Research associate - Oct. 2002 - Mar. 2007
Hoshi University, Institute of Medicinal Chemistry, Laboratory of Biological resources, Research assistant - Oct. 2001 - Sep. 2002
National Institute of Health Sciences, Division of Food Additives - Apr. 1994 - Jul. 2001
Hoshi University, Department of pharmacy, Laboratory of microbiology, Research assistant
■Research activity information
Award
Paper
- Distinguishing two distinct types of salivary extracellular vesicles: a potential tool for understanding their pathophysiological roles.
Yuko Ogawa; Yuri Miura; Mamoru Ikemoto; Atsushi Ohnishi; Yoshikuni Goto; Kazuma Aoki; Yuki Motokurumada; Yoshihiro Akimoto; Tamao Endo; Masafumi Tsujimoto; Ryohei Yanoshita
Frontiers in molecular biosciences, 2024, [Reviewed]
Extracellular vesicles (EVs), which are found in almost all cells and human body fluids, are currently being studied as a source of pathophysiological information. Previously, we demonstrated that at least two types of EVs can be isolated from human whole saliva (WS) using enzymatic activity of dipeptidyl peptidase IV (DPP IV) as a marker for differentiating the EV subsets. In the present study, EV fractions, termed EV-I 20 k-ppt and EV-II 100 k-ppt, were prepared by a combination of size-exclusion chromatography of improved condition and sequential centrifugation. The EV-I 20 k-ppt fraction contained medium/large EVs with a diameter of 100-1,000 nm, including aminopeptidase N (APN), mucin 1, ezrin, and Annexin A1. EV-II 100 k-ppt contained small EVs with a diameter of 20-70 nm, with DPP IV and CD9, programmed cell death 6-interacting protein, and tumor susceptibility gene 101 as characteristic proteins. Proteomic analyses also revealed distinctive repertoires of constituent proteins. Immunoprecipitation of several membrane proteins of the EVs with respective antibodies suggested their differential local membrane environment between the two types of salivary vesicles. Thus, we identified two distinctive types of EVs, one is APN/MUC1- rich EVs (EV-I, large/medium EVs) and the other is DPP IV/CD9-rich EVs (EV-II, small EVs). Furthermore, analysis of the binding of the EVs to coronavirus spike proteins showed that EV-II 100 k-ppt, but not EV-I 20 k-ppt, significantly bound to the spike protein of Middle East respiratory syndrome coronavirus (MERS-CoV). Finally, we developed a simple method to prepare two distinctive EVs from only 1 mL of human WS using sequential immunoprecipitation. Elucidating the features and functions of these two types of salivary EVs may help us understand their pathophysiological roles in the oral cavity and gastrointestinal tract. - Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions
Yuko Ogawa; Yoshihiro Akimoto; Mamoru Ikemoto; Yoshikuni Goto; Anna Ishikawa; Sakura Ohta; Yumi Takase; Hayato Kawakami; Masafumi Tsujimoto; Ryohei Yanoshita
Biochemistry and Biophysics Reports, Sep. 2021, [Reviewed]
Background: Extracellular vesicles (EVs) have been isolated from various sources, including primary and cultured cell lines and body fluids. Previous studies, including those conducted in our laboratory, have reported the stability of EVs under various storage conditions. Methods: EVs from human whole saliva were separated via size-exclusion chromatography. To simulate the effects of gastric or intestinal fluids on the stability of EVs, pepsin or pancreatin was added to the samples. Additionally, to determine the effect of bile acids, sodium cholate was added. The samples were then subjected to western blotting, dynamic light scattering, and transmission electron microscopy analyses. In addition, the activity of dipeptidyl peptidase (DPP) IV retained in the samples was examined to monitor the stability of EVs. Results: Under acidic conditions, with pepsin mimicking the milieu of the stomach, the EVs remained stable. However, they partially lost their membrane integrity in the presence of pancreatin and sodium cholate, indicating that they may be destabilized after passing through the duodenum. Although several associated proteins, such as mucin 5B and CD9 were degraded, DPP IV was stable, and its activity was retained under the simulated gastrointestinal conditions. Conclusion: Our data indicate that although EVs can pass through the stomach without undergoing significant damage, they may be disrupted in the intestine to release their contents. The consistent delivery of active components such as DPP IV from EVs into the intestine might play a role in the efficient modulation of homeostasis of the signal transduction pathways occurring in the gastrointestinal tract. - Contribution of the exosome-associated form of secreted endoplasmic reticulum aminopeptidase 1 to exosome-mediated macrophage activation
Yoshikuni Goto; Yuko Ogawa; Hiroki Tsumoto; Yuri Miura; Takahiro J. Nakamura; Kenji Ogawa; Yoshihiro Akimoto; Hayato Kawakami; Tamao Endo; Ryohei Yanoshita; Masafumi Tsujimoto
Biochimica et Biophysica Acta - Molecular Cell Research, 01 Jun. 2018, [Reviewed] - Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes
Nahoko Kumeda; Yuko Ogawa; Yoshihiro Akimoto; Hayato Kawakami; Masafumi Tsujimoto; Ryohei Yanoshita
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Aug. 2017, [Reviewed] - Migration of nonylphenol and plasticizers from polyvinyl chloride stretch film into food simulants, rapeseed oil, and foods
Yoko Kawamura; Yuko Ogawa; Motoh Mutsuga
FOOD SCIENCE & NUTRITION, May 2017, [Reviewed] - Next-Generation Sequencing of Protein-Coding and Long Non-protein-Coding RNAs in Two Types of Exosomes Derived from Human Whole Saliva
Yuko Ogawa; Masafumi Tsujimoto; Ryohei Yanoshita
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Sep. 2016, [Reviewed] - Involvement of Phenylalanine 297 in the Construction of the Substrate Pocket of Human Aminopeptidase B
Atsushi Ohnishi; Jobu Watanabe; Yuko Ogawa; Yoshikuni Goto; Akira Hattori; Masafumi Tsujimoto
BIOCHEMISTRY, Oct. 2015, [Reviewed] - Role of glutamine-169 in the substrate recognition of human aminopeptidase B
Yuko Ogawa; Atsushi Ohnishi; Yoshikuni Goto; Yoshiki Sakuma; Jobu Watanabe; Akira Hattori; Masafumi Tsujimoto
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, Jun. 2014, [Reviewed] - Small RNA Transcriptomes of Two Types of Exosomes in Human Whole Saliva Determined by Next Generation Sequencing
Yuko Ogawa; Yoshitaka Taketomi; Makoto Murakami; Masafumi Tsujimoto; Ryohei Yanoshita
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jan. 2013, [Reviewed] - Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
Yuko Ogawa; Yuri Miura; Akira Harazono; Masami Kanai-Azuma; Yoshihiro Akimoto; Hayato Kawakami; Teruhide Yamaguchi; Tosifusa Toda; Tamao Endo; Masayoshi Tsubuki; Ryohei Yanoshita
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jan. 2011, [Reviewed] - Selective accumulation of a novel antimalarial rhodacyanine derivative, SSJ-127, in an organelle of Plasmodium berghei
Mayumi Ikegami-Kawai; Chika Arai; Yuko Ogawa; Ryohei Yanoshita; Masataka Ihara
BIOORGANIC & MEDICINAL CHEMISTRY, Nov. 2010, [Reviewed] - Molecular cloning and characterization of ecto-5 '-nucleotidase from the venoms of Gloydius blomhoffi
Yuko Ogawa; Nobuhiro Murayama; Ryohei Yanoshita
TOXICON, Sep. 2009, [Reviewed] - Exosome-like vesicles with dipeptidyl peptidase IV in human saliva
Yuko Ogawa; Masami Kanai-Azuma; Yoshihiro Akimoto; Hayato Kawakami; Ryohel Yanoshita
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jun. 2008, [Reviewed] - Exosome-like vesicles in Gloydius blomhoffii blomhoffii venom
Yuko Ogawa; Masami Kanai-Azuma; Yoshihiro Akimoto; Hayato Kawakami; Ryohei Yanoshita
TOXICON, May 2008, [Reviewed] - Characterization and cDNA cloning of aminopeptidase A from the venom of Gloydius blomhoffi brevicaudus
Yuko Ogawa; Nobuhiro Murayama; Yoshiaki Fujita; Ryohel Yanoshita
TOXICON, Jun. 2007, [Reviewed] - Characterization and cDNA cloning of dipeptidyl peptidase IV from the venom of Gloydius blomhoffi brevicaudus
Yuko Ogawa; Yoshie Mamura; Nobuhiro Murayama; Ryohei Yanoshita
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, Sep. 2006, [Reviewed] - Estrogenic activities of chemicals related to food contact plastics and rubbers tested by the yeast two-hybrid assay
Y Ogawa; Y Kawamura; C Wakui; M Mutsuga; T Nishimura; K Tanamoto
FOOD ADDITIVES AND CONTAMINANTS, Apr. 2006, [Reviewed] - Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus)
R Yanoshita; Y Ogawa; N Murayama; T Omori-Satoh; K Saguchi; S Higuchi; O Khow; L Chanhome; Y Samejima; Sitprija, V
TOXICON, Mar. 2006, [Reviewed] - On a salmon (Onchorhynchus keta) liver RNase, belonging to RNase T2 family: Primary structure and some properties
R Suzuki; S Kanno; Y Ogawa; M Iwania; T Tsuji; K Ohgi; M Irie
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Feb. 2005, [Reviewed] - Complete amino acid sequence and phylogenetic analysis of a long-chain neurotoxin from the venom of the African banded water cobra, Boulengerina annulata
Y Ogawa; R Yanoshita; U Kuch; Y Samejima; D Mebs
TOXICON, Jun. 2004, [Reviewed] - Isolation, toxicity and amino terminal sequences of three major neurotoxins in the venom of Malayan krait (Bungarus candidus) from Thailand
O Khow; L Chanhome; T Omori-Satoh; Y Ogawa; R Yanoshita; Y Samejima; U Kuch; D Mebs; Sitprija, V
JOURNAL OF BIOCHEMISTRY, Dec. 2003, [Reviewed] - Estrogenic activities of UV stabilizers used in food contact plastics and benzophenone derivatives tested by the yeast two-hybrid assay
Y Kawamura; Y Ogawa; T Nishimura; Y Kikuchi; J Nishikawa; T Nishihara; K Tanamoto
JOURNAL OF HEALTH SCIENCE, Jun. 2003, [Reviewed] - Effect of replacing the aspartic acid/glutamic acid residues of bullfrog sialic acid binding lectin with asparagine/glutamine and arginine on the inhibition of cell proliferation in murine leukemia P388 cells
Y Ogawa; M Iwama; K Ohgi; T Tsuji; M Irie; T Itagaki; H Kobayashi; N Inokuchi
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jun. 2002, [Reviewed] - Enzymatic properties of sialic acid binding lectin from Rana catesbeiana modified with a water-soluble carbodiimide in the presence of various nucleophiles
M Iwama; Y Ogawa; K Ohgi; T Tsuji; M Irie
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Dec. 2001, [Reviewed] - Effect of modification of the carboxyl groups of the sialic acid binding lectin from bullfrog (Rana catesbeiana) oocyte on anti-tumor activity
M Iwama; Y Ogawa; N Sasaki; K Nitta; Y Takayanagi; K Ohgi; T Tsuji; M Irie
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Sep. 2001, [Reviewed] - Amino acid sequence and characterization of a rice bran ribonuclease
M Iwama; Y Ogawa; M Yamagishi; T Itagaki; N Inokuchi; T Koyama; R Imai; K Ohgi; T Tsuji; M Irie
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jul. 2001, [Reviewed] - A protease sensitive region of plant and animal ribonucleases belonging to the RNase T2 family
M Iwama; A Kusano; Y Ogawa; K Ohgi; M Irie
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Jun. 1998, [Reviewed] - Bovine spleen acid ribonuclease is a member of the RNase T2 family.
1997, [Reviewed] - Enzymatic activities of several K108 mutants of ribonuclease (RNase) isolated from Rhizopus niveus
K Ohgi; M Iwama; Y Ogawa; C Hagiwara; E Ono; R Kawaguchi; C Kanazawa; M Irie
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Aug. 1996, [Reviewed]
MISC
- LPS-binding proteins in human salivary extracellular vesicles regulate the activation of macrophages.
小川裕子; 三浦ゆり; 後藤芳邦; 池本守; 秋元義弘; 遠藤玉夫; 矢ノ下良平
日本薬学会年会要旨集(Web), 2023 - アミノペプチダーゼに着目したヒト唾液由来細胞外小胞の性状解析
Mar. 2022 - Specification of extracellular vesicles characterized by their membrane proteins, APN/mucin1 or DPP IV/CD9
小川裕子; 三浦ゆり; 大西敦; 後藤芳邦; 青木一真; 池本守; 本車田悠希; 堤周平; 長島茉央; 廣谷莉花; 武井亮太朗; 秋元義弘; 遠藤玉夫; 矢ノ下良平
日本唾液腺学会誌, 2022 - Stability of human salivary extracellular vesicles under simulated gastric and intestinal conditions
小川裕子; 石川杏奈; 太田櫻; 池本守; 後藤芳邦; 秋元義弘; 川上速人; 辻本雅文; 矢ノ下良平
日本薬学会年会要旨集(Web), 2021 - ヘビ毒の膜結合性酵素の性状解析とcDNAクローニング
小川 裕子; 村山 信浩; 矢ノ下 良平
日本薬学会年会要旨集, Mar. 2008
(公社)日本薬学会 - タンビマムシ毒に含まれるアミノペプチダーゼの性状解析
小川 裕子; 矢ノ下 良平; 村山 信浩
日本薬学会年会要旨集, Mar. 2006
(公社)日本薬学会 - 2種のアマガサ蛇の毒に含まれるβ-ブンガロトキシンのcDNAクローニング
小川 裕子; 村山 信浩; 佐口 健一; 矢ノ下 良平; 佐藤 保; 樋口 成定; 鮫島 勇次
日本薬学会年会要旨集, Mar. 2004
(公社)日本薬学会 - 中国マムシ毒由来ジペプチジルアミノペプチダーゼIVの性状解析とcDNAクローニング
小川 裕子; 作田 朋子; 増井 由佳; 矢ノ下 良平; 村山 信浩; 樋口 成定; 鮫島 勇次
日本薬学会年会要旨集, Mar. 2004
(公社)日本薬学会
Lectures, oral presentations, etc.
Affiliated academic society
Research Themes
- Development of Next-Generation Artificial Saliva with Immunostimulating Ability Using Extracellular Vesicles Derived from Human Saliva
Grant-in-Aid for Scientific Research (C)
Teikyo Heisei University
01 Apr. 2023 - 31 Mar. 2026 - Development of autovaccine for periodontal disease using human salivary exosomes with immune activity
Grant-in-Aid for Scientific Research (C)
Teikyo Heisei University
Apr. 2020 - Mar. 2023 - エキソソームによる粘膜免疫賦活機構の解明
Apr. 2016 - Mar. 2019 - M1 アミノペプチダーゼ機能異常と自己免疫疾患病態の分子機構
Apr. 2013 - Mar. 2016 - アミノペプチダーゼ内包エキソソームによる免疫活性化カスケードの分子機構とその制御
Apr. 2013 - Mar. 2016 - 医薬品開発に応用できる生体成分由来ナノキャリアの開発
Oct. 2008 - Sep. 2009
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