Ryohei Yanoshita

Graduate School of Information Sciences,Major of Information SciencesDean of Graduate School/Chair of Program/Professor
Faculty of Pharmaceutical Sciences,Department of Pharmaceutical SciencesProfessor
Last Updated :2025/10/07

■Researcher basic information

Degree

  • (BLANK), The University of Tokyo
  • (BLANK), The University of Tokyo

Research Keyword

  • saliva
  • exosome

Field Of Study

  • Life sciences, Pharmaceuticals - health and biochemistry

■Career

Career

  • Teikyo Heisei University, Professor

Educational Background

  • Mar. 1987, The University of Tokyo
  • Mar. 1982, The University of Tokyo, Faculty of Pharmaceutical Sciences

■Research activity information

Paper

MISC

Research Themes

  • Mechanism of mucosal immunity activation by exosome
    Grant-in-Aid for Scientific Research (C)
    Teikyo Heisei University
    21 Oct. 2016 - 31 Mar. 2019
    Human whole saliva contains exosomes that have mucosal immunomodulatory potential. Immune-related proteins such as DPP IV and IgA, salivary proteins such as mucin 5B, and lipopolysaccharide (LPS) derived from oral bacteria were associated with surface of the exosomes.
    Mucin 5B was digested with gastrointestinal enzymes. Although IgA and LPS were dissociated by size-exclusion chromatography, a part of them was tightly associated with the exosomes. Salivary exosome without the surface molecules increased production of nitric oxide (NO) from murine macrophages. In addition, we found that DPP IV may contribute to the NO production by collaboration with LPS. Salivary exosome is presumed to suppress the excessive activation in oral cavity and cause the activation of mucosal immunity after exposure to the gastrointestinal condition.
  • Molecular mechanism and regulation of immune activation cascade by aminopeptidase contained exosome.
    Grant-in-Aid for Scientific Research (C)
    Teikyo Heisei University
    01 Apr. 2013 - 31 Mar. 2016
    Human whole saliva contains two types of extracellular vesicles. One type highly expresses dipeptidyl peptidase-4 (DPP4) and the other type rarely expresses them. We designate former as DPP IV-exosome (DPP4exo), and later as deficient DPP IV-exosome (dDPP4exo). DPP4exo contained lipopolysaccharide (LPS) abundantly, and dDPP4exo contained it slightly. Although large amount of LPS interacted weakly with DPP4exo or existed separately forming micelle, part of LPS bind DPP4exo tightly. DPP4exo alone did not activate murine macrophage, however, DPP4exo with interferon-gamma produce high level of nitric oxide (NO) from murine macrophage. dDPP4exo did not cause NO production, even when interferon-gamma was added. DPP4exo is presumed to induce NO production from macrophage, when inflammation caused by bacterial infection, and associated with increased levels of interferon-gamma in oral cavity.
  • exosome