Yuichi Wada(Vice President)｜Teikyo Heisei University Researcher Information Researcher Search by Organization

Name

Author

Position

Affiliation

Research Areas

Yuichi Wada

Vice President	（Chiba Campus and Sports Affairs）
Faculty of Health Care and Medical Sports,Department of Medical Sports,Paramedic Course	Dean of Faculty/Chair of Department/Professor
Faculty of Health Care and Medical Sports,Department of Medical Sports,Trainer and Sports Course	Dean of Faculty/Chair of Department/Professor
Faculty of Health Care and Medical Sports,Department of Medical Sports,Athlete Course	Dean of Faculty/Chair of Department/Professor
Faculty of Health Care and Medical Sports,Department of Medical Sports,Animal Medical Course	Dean of Faculty/Chair of Department/Professor

Last Updated :2025/10/07

■Researcher basic information

Research Keyword

Mechanical stimuli

Cell membrane

Gene cloning

Differential display

ATDC5

Mechano-receptor

Field Of Study

Life sciences, Orthopedics

Life sciences, General surgery, pediatric surgery

■Career

Career

Jan. 2005 - Present
Teikyo University, Chiba Medical Center

2003

1996

Educational Background

Apr. 1977 - Mar. 1983, Chiba University, School of Medicine

Member History

1999 - Present

Present

Present

Present

2004 - 2008

■Research activity information

Award

2004

2004

2003

2003
Japan

2001

1997
Japan

1995

Paper

Quantitative evaluation of fatty degeneration of the supraspinatus and infraspinatus muscles using T2 mapping
Keisuke Matsuki; Atsuya Watanabe; Shunsuke Ochiai; Tomonori Kenmoku; Nobuyasu Ochiai; Takayuki Obata; Tomoaki Toyone; Yuichi Wada; Toshiyuki Okubo
Journal of Shoulder and Elbow Surgery, May 2014, [Reviewed]
DOI URL Scopus URL Scopus Citedby URL

In vivo 3D analysis of clavicular kinematics during scapular plane abduction: comparison of dominant and non-dominant shoulders.
Keisuke Matsuki; Kei O Matsuki; Shang Mu; Tomonori Kenmoku; Satoshi Yamaguchi; Nobuyasu Ochiai; Takahisa Sasho; Hiroyuki Sugaya; Tomoaki Toyone; Yuichi Wada; Kazuhisa Takahashi; Scott A Banks
Gait & posture, 2014, [Reviewed]
The purpose of this study was to evaluate side-to-side differences in three-dimensional clavicle kinematics in normal shoulders during dynamic scapular plane elevation using model-image registration techniques. Twelve healthy males with a mean age of 32 years (range, 27-36 years old) were enrolled in this study. Clavicle rotations were computed with bilateral fluoroscopic images and CT-derived bone models using model-image registration techniques and compared between dominant and nondominant shoulders. There was no difference in retraction between both shoulders. The clavicle in dominant shoulders was less elevated during abduction than in nondominant shoulders (P=0.03). Backward rotation angles of dominant shoulders were significantly smaller than those of nondominant shoulders throughout the activity (P=0.03). Clavicular kinematics during scapular plane abduction were different according to hand-dominance.
DOI URL

MRIで大腿骨顆部に認められる小児の軟骨下骨病変の頻度、好発部位の検討　　　　　　　　　　　　　　　
Jun. 2013

T1rhoマッピングとT2マッピングの軟骨変性評価能の検討　　　　　　　　　　　　　　　
Jun. 2013

T1ρマッピングとT2マッピングの軟骨変性評価能の検討
Mar. 2013

T1ρマッピングを用いた膝関節軟骨変性の経時的変化の検討
Mar. 2013

小児の膝関節MRIで大腿骨顆部に認められる軟骨下骨病変の頻度、好発部位の検討
Mar. 2013

Asymmetrical pedicle subtraction osteotomy for rigid degenerative lumbar kyphoscoliosis.
Tomoaki Toyone; Ryutaro Shiboi; Tomoyuki Ozawa; Kunimasa Inada; Toshiyuki Shirahata; Koya Kamikawa; Atsuya Watanabe; Keisuke Matsuki; Shunsuke Ochiai; Taku Kaiho; Yoshiki Morikawa; Kitahara Sota; Aoki Yasuchika; Inoue Gen; Orita Sumihisa; Seiji Ohtori; Kazuhisa Takahashi; Yuichi Wada
Spine, 01 Oct. 2012
STUDY DESIGN: A prospectively study. OBJECTIVE: Our objective was to clarify the safety and efficacy of asymmetrical pedicle subtraction osteotomy (PSO) in the treatment of severe adult lumbar deformities prospectively. SUMMARY OF BACKGROUND DATA: Vertebral wedge osteotomy provides good correction of kyphosis but has rarely been applied to degenerative lumbar kyphoscoliosis. METHODS: A total of 14 patients who had undergone corrective osteotomy were enrolled. The average age at PSO was 67 years (range, 45-76 yr). The minimum follow-up was 2 years. Patient questionnaires were administered prospectively. Radiographical parameters including sagittal and coronal balance were analyzed. RESULTS: Average operative time was 310 minutes (range, 254-375 min). Average blood loss was 1090 mL (range, 700-2900 mL).Mean preoperative lumbar lordosis improved from -3° to 42° at the final follow-up, and sagittal balance improved from 12 to 3 cm, respectively. Mean lumbar scoliosis improved from 40° to 12°, and coronal offset improved from 3 to 1 cm, respectively. There was also statistically significant improvement from preoperative to final evaluation in all clinical domains. There were 4 complications: 1 dural tear, 2 hook dislodgements at the cephalad side requiring revision instrumentation, and 1 rod breakage not requiring surgical intervention. Overall, all 14 patients were satisfied with their surgical management and would choose to repeat the procedure. CONCLUSION: Our data suggest that the surgical procedure of asymmetrical PSO is to correct the scoliosis, to restore the lumbar lordosis by way of convex-sided posterolateral wedge osteotomy, and may go a long way toward solving the problems of rigid lumbar degenerative kyphoscoliosis.

T1rho mappingおよび関節鏡による軟骨変性評価能の比較
Mar. 2012

体位による大動脈と脊椎との位置関係の変化
Mar. 2012

Dynamic in vivo glenohumeral kinematics during scapular plane abduction in healthy shoulders
Keisuke Matsuki; Kei O. Matsuki; Satoshi Yamaguchi; Nobuyasu Ochiai; Takahisa Sasho; Hiroyuki Sugaya; Tomoaki Toyone; Yuichi Wada; Kazuhisa Takahashi; Scott A. Banks
Journal of Orthopaedic and Sports Physical Therapy, 2012, [Reviewed]
DOI URL

MRIによる軟骨変性の定量的評価法とModified Outerbridge分類との比較
Aug. 2011

三次元培養軟骨組織に対するMRI評価の検討　　　　　　　　　　　　　　　
Aug. 2011

T2マッピングを用いた新鮮前十字靱帯損傷症例における軟骨損傷の評価　　　　　　　　　　　　　　　
Jun. 2011

新鮮前十字靱帯損傷症例における軟骨損傷の評価
Mar. 2011

T1rho mappingおよびT2 mappingの軟骨変性における評価能の比較
Mar. 2011

変形性膝関節症症例におけるCOX-2選択的阻害薬の軟骨変性予防効果に関する検討
Mar. 2011

MRI T2強調像による椎間板変性度評価の観察者内変動、観察者間変動、および日内変動に関する検討
Mar. 2011

軟骨変性評価におけるT1rho mapping及びT2mappingの比較　　　　　　　　　　　　　　　
Feb. 2011

新鮮前十字靱帯損傷症例における大腿骨外側顆の軟骨損傷の評価　　　　　　　　　　　　　　　
Feb. 2011

MRIにおける椎間板変性度分類法の有用性の検討　　　　　　　　　　　　　　　
Jan. 2011

Effects of autologous platelet-rich plasma on cell viability and collagen synthesis in injured human anterior cruciate ligament.
Louay Fallouh; Koichi Nakagawa; Takahisa Sasho; Momoko Arai; Sota Kitahara; Yuichi Wada; Hideshige Moriya; Kazuhisa Takahashi
The Journal of bone and joint surgery. American volume, 15 Dec. 2010
BACKGROUND: Platelet-rich plasma is a fraction of plasma in which platelets are concentrated. It is reported to represent a source of multiple growth factors that promote tissue repair. In anticipation of the eventual testing of platelet-rich plasma in anterior cruciate ligament (ACL)-deficient patients, we examined the effect of autologous platelet-rich plasma on human ACL cell function in vitro. METHODS: Fresh blood and ACL remnants were obtained from four patients who underwent ACL reconstruction surgery. Platelet-poor plasma and platelet-rich plasma were prepared from the blood samples. The concentrations of various growth factors in each preparation were tested with use of enzyme-linked immunosorbent assays. Isolated ACL cells were cultured in the presence of 5% fetal bovine serum, 5% platelet-poor clot releasate, 5% platelet-rich clot releasate, or 10% platelet-rich clot releasate. Platelet-rich plasma and platelet-poor plasma releasates were applied to the ACL cells from the same patient autologously. Cell viability and collagen synthesis in each group were analyzed, and semiquantitative gene-expression assays for type-I and III collagen were also performed. RESULTS: The concentrations of the main growth factors (transforming growth factor-beta, platelet-derived growth factor, epidermal growth factor, and vascular endothelial growth factor) were much higher in platelet-rich clot releasate than in platelet-poor clot releasate. In vitro treatment of ACL cells with platelet-rich clot releasate resulted in a significant increase in cell number compared with platelet-poor clot releasate. Total collagen production by the platelet-rich clot releasate-treated cells was significantly higher than that of the platelet-poor clot releasate-treated cells only because of enhanced cell proliferation. There was no significant effect of platelet-rich clot releasate treatment on gene expression for type-I collagen, but expression of type-III collagen was significantly enhanced by the treatment with platelet-rich clot releasate. CONCLUSIONS: These results suggest that autologous platelet-rich plasma can enhance ACL cell viability and function in vitro.
DOI URL

変形性膝関節症の病期診断における遅延相軟骨造影MRIの有用性の検討
Apr. 2010

Objective assessments of medial osteoarthritic knee severity by MRI: new computer software to evaluate femoral condyle contours
Nobuyasu Ochiai; Takahisa Sasho; Masamichi Tahara; Atsuya Watanabe; Kei Matsuki; Satoshi Yamaguchi; Yoichi Miyake; Toshiya Nakaguchi; Yuichi Wada; Hideshige Moriya
International Orthopaedics (SICOT), 2010, [Reviewed]
DOI URL

MRIを用いた関節軟骨変性度の定量的評価単純X線病期分類との比較　　　　　　　　　　　　　　　
Oct. 2009

自家培養軟骨細胞移植による軟骨修復術後の再生軟骨の質的評価 MRIによる長期経過観察　　　　　　　　　　　　　　　
Oct. 2009

MEDAL法を用いた骨格筋内脂肪含有率、水分含有率の評価
Aug. 2009

軟骨変性・再生の評価技法再生軟骨の質的評価における遅延相軟骨造影MRIおよびT2マッピングの有用性の検討
Aug. 2009

遅延相軟骨造影MRIを用いた関節軟骨変性度の定量的評価
Mar. 2009

軟骨中のglycosaminoglycan濃度評価が可能なMRI撮像法を用いた軟骨変性度の定量的評価　　　　　　　　　　　　　　　
Feb. 2009

T2マッピングによる椎間板線維輪断裂の評価椎間板造影との比較　　　　　　　　　　　　　　　
Feb. 2009

T2マッピングを用いた膝関節軟骨変性度の定量的評価　　　　　　　　　　　　　　　
Feb. 2009

細切軟骨片との共存移植が滑膜細胞の軟骨分化に与える影響
2009, [Reviewed]
URL

RGD peptide-induced cell death of chondrocytes and synovial cells.
Keisuke Matsuki; Takahisa Sasho; Koichi Nakagawa; Masamichi Tahara; Kaori Sugioka; Nobuyasu Ochiai; Shuhei Ogino; Yuichi Wada; Hideshige Moriya
Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, Nov. 2008
BACKGROUND: Small peptides including the Arg-Gly-Asp (RGD) motif have been used in studies on cell-extracellular matrix (ECM) attachment due to their ability to disturb integrin-mediated attachment on the cell surface. As another biological action of RGD peptides, several reports have shown that RGD peptides are incorporated into cytoplasm and induce apoptosis by direct activation of caspase-3. This study evaluated the effect of RGD peptides on chondrocytes and synovial cells and studied the involvement of caspases. METHODS: Chondrocytes and synovial cells were isolated and cultured from the knee joints of New Zealand White rabbits. Cells were incubated in serum-free medium with peptides (RGD, RGDS, GRGDSP, GRGDNP, RGES), and the survival rates were evaluated. The rate of apoptotic cells was measured by flow cytometry in cells treated with RGDS, GRGDSP, and RGES. Caspase-3, -8 and -9 activity was measured in cells treated with RGDS and GRGDSP. Osteochondral explants harvested from rabbits were also incubated with RGD peptides (RGDS, GRGDSP, and GRGDNP), and the survival rate of chondrocytes was evaluated. RESULTS: The survival rate of cultured chondrocytes was significantly decreased in the GRGDSP- and GRGDNP-treated groups. The survival rate of synovial cells was significantly decreased with four of the RGD peptides (RGD, RGDS, GRGDSP, and GRGDNP) at 5 mM, and in the RGDS- and GRGDSP-treated groups at 1 mM. Flow cytometric assay revealed increases of apoptotic chondrocytes with GRGDSP and increases of apoptotic synovial cells with RGDS and GRGDSP. Caspase-3 was activated in chondrocytes treated with GRGDSP and it was also activated in synovial cells treated with RGDS and GRGDSP. Caspases-8 and -9 were not activated in chondrocytes or in synovial cells. The survival rate of chondrocytes in explants decreased in the superficial layer with all three RGD peptides (RGDS, GRGDSP, and GRGDNP) and in the middle layer with GRGDSP. CONCLUSIONS: RGD peptides induced apoptosis in cultured chondrocytes as well as in cells in cartilage explants and synovial cells, presumably through direct activation of caspase-3.
DOI URL

In vivo maturation of scaffold-free engineered articular cartilage on hydroxyapatite.
Sota Kitahara; Koichi Nakagawa; Robert L Sah; Yuichi Wada; Tetsuro Ogawa; Hideshige Moriya; Koichi Masuda
Tissue engineering. Part A, Nov. 2008
Tissue engineering is a promising approach, not only for cartilage, but also for osteochondral repair. Recent studies have demonstrated that scaffold-free cartilaginous tissue can be engineered using the alginate-recovered-chondrocyte (ARC) method. This method has also been applied to form osteochondral tissue using bovine articular chondrocytes and coralline hydroxyapatite (HA). The purpose of this study was to test whether osteochondral tissue, fabricated in vitro using the ARC method combined with a block of HA, would undergo maturation in vivo using a subcutaneous model in immunodeficient mice. Articular chondrocytes were isolated from the cartilage of New Zealand white rabbits and cultured in alginate beads. The cells with their associated matrix were recovered by dissolving the alginate beads with a sodium citrate buffer, resuspended in media and seeded onto a porous HA block. After 4 weeks of culture, some samples were analyzed, and others were implanted into subcutaneous pockets in nude mice. The analysis involved removing the cartilage portion of the de novo-formed ARC-HA graft and performing biochemical and histological examinations. Some samples were subjected to nondecalcified histology. Histological and immunohistochemical analyses of cartilaginous tissue were performed at 0, 2, 4, and 8 weeks after implantation. Biochemical characteristics were examined at 0, 4, and 8 weeks. The size and shape of the implanted ARC osteochondral tissue changed with time. The histological and immunohistochemical examination of the tissue revealed that it contained a cartilage-like matrix that stained strongly with Toluidine blue and for collagen type II. The proteoglycan (PG) content had increased significantly at 4 weeks from baseline. However, by 8 weeks, the PG content had decreased from 4 weeks. The results presented here represent a possible approach to form a tissue-engineered osteochondral implant. Further studies are needed to improve biomechanical properties of the osteochondral implant to be suitable for surgical transplantation.
DOI URL

Spontaneous recurrent hemarthrosis of the knee in the elderly: arthroscopic treatment and etiology.
Takahisa Sasho; Shuhei Ogino; Hiroaki Tsuruoka; Koichi Nakagawa; Nobuyasu Ochiai; Ryota Nagashima; Hideshige Moriya; Atsuya Watanabe; Yuichi Wada; Kazuhisa Takahashi
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association, Sep. 2008
PURPOSE: To elucidate the etiology of and find a preferable surgical treatment for spontaneous recurrent hemarthrosis in osteoarthritic knee joints arthroscopically. METHODS: Nineteen patients referred to our institution from affiliate hospitals between April 1998 and October 2006 were involved in this study. Their demographics, preoperative radiographic findings, preoperative magnetic resonance imaging (MRI) findings, arthroscopic findings and procedures performed, the patient's medical history, and the postoperative clinical course were retrospectively reviewed. RESULTS: There were 9 male and 10 female patients with average age of 61.9 years (range, 41 to 83 yrs). The average number of joint aspirations before surgery was 5.4. The average time from onset to arthroscopy was 10 months. Radiographs showed 2 knees with isolated lateral compartment osteoarthritis (OA), one with isolated patellofemoral (PF) OA, 14 with medial and lateral compartment OA, and 2 with tricompartmental OA. Classifying them according to the dominant compartment, 6 knees were medial-dominant OA, 11 lateral-dominant OA, and 2 PF-dominant OA. The MRI scans revealed 18 grade III lateral menisci and 1 grade II lateral menisci. Even with 6 medial-dominant OAs, lateral meniscal involvement was more obvious than medial meniscal involvement on MRI. Subtotal lateral meniscectomy accompanied with coagulation of the bleeding points was performed on 17 cases. For 2 PF OA cases, synovectomy and a histologic examination of synovium were performed. Remission was obtained for 18 cases. The unsuccessful case had cirrhosis of the liver. CONCLUSIONS: A majority of the patients (17 of 19) had degenerative torn lateral menisci confirmed with MRI and at arthroscopy. Successful outcomes were achieved by meniscectomy and coagulation. Most so-called spontaneous recurrent hemarthroses in OA knee joints appear to be attributable to torn lateral menisci. LEVEL OF EVIDENCE: Level IV, therapeutic case series.
DOI URL

再生運動器評価法の開発と臨床応用軟骨組織遅延相軟骨造影MRIによる関節軟骨のグリコサミノグリカン評価
Aug. 2008

三次元培養軟骨のMRIによる多角的組織組成評価
Aug. 2008

変形性関節症の基礎と臨床変形性関節症研究の画像診断の最前線　　　　　　　　　　　　　　　
Apr. 2008

椎間板髄核のT2日内変動に関する検討椎間板変性度、高位、部位による差異　　　　　　　　　　　　　　　
Feb. 2008

急性期前十字靱帯断裂症例における大腿骨骨挫傷と軟骨損傷との関連 T2マッピングによる評価　　　　　　　　　　　　　　　
Feb. 2008

T2マッピングを用いた膝関節軟骨変性度の定量的評価　　　　　　　　　　　　　　　
Feb. 2008

T2マッピングを用いた関節軟骨変性度の定量的評価　　　　　　　　　　　　　　　
Oct. 2007

Extracorporeal shock wave therapy improves motor dysfunction and pain originating from knee osteoarthritis in rats.
N Ochiai; S Ohtori; T Sasho; K Nakagawa; K Takahashi; N Takahashi; R Murata; K Takahashi; H Moriya; Y Wada; T Saisu
Osteoarthritis and cartilage, Sep. 2007
OBJECTIVE: Although there have been several reports on the use of extracorporeal shock wave therapy (ESWT), the efficacy of ESWT for knee osteoarthritis (OA) has not been clarified. The aim of this study is to investigate the effect of ESWT on OA in a rat knee model. METHODS: The rats were divided into three groups: (1) control, (2) OA, and (3) ESWT (knee OA+shock wave therapy). Behavioral analysis consisted of measuring the duration of walking on a treadmill. The expression of calcitonin gene-related peptide (CGRP) in dorsal root ganglion (DRG) neurons innervating the knee using immunohistochemistry was examined in the three groups at their peak time point on the treadmill. RESULTS: Walking duration was significantly extended 4, 7 and 14 days after ESWT in rats with knee OA (peak time point: 4 days), again decreasing by days 21 and 28. Immunohistochemical studies revealed that the OA group had significantly higher percentages of CGRP positive neurons in the DRG than were found in the control group. In addition, ESWT reduced the ratio of CGRP positive DRG neurons in the OA model. CONCLUSION: The improvement in walking ability and the reduction of CGRP positive neurons in DRG indicates that ESWT is a useful treatment for knee OA.

明らかな皮膚病変が無い下腿皮下基底細胞癌の1例
Aug. 2006

【関節軟骨の再生と修復】臨床に向けた応用単層培養を用いた自家培養軟骨移植
Apr. 2006

骨頭下骨折を生じた大腿骨頭軟骨下脆弱性骨折の一例
Aug. 2005

Time course evaluation of reparative cartilage with MR imaging after autologous chondrocyte implantation
Atsuya Watanabe; Yuichi Wada; Takayuki Obata; Takahisa Sasho; Takuya Ueda; Mitsuru Tamura; Hiroo Ikehira; Hideshige Moriya
Cell Transplantation, 2005, [Reviewed]
DOI URL Scopus URL Scopus Citedby URL

Arthroscopic posteromedial release for osteoarthritic knees with flexion contracture
Hideshige Moriya; Takahisa Sasho; Sakae Sano; Yuichi Wada
Arthroscopy - Journal of Arthroscopic and Related Surgery, Dec. 2004
DOI URL

特発性大腿骨顆部骨壊死に対する鏡視下デブリードマン　　　　　　　　　　　　　　　
Oct. 2004

Deterioration of long-term clinical results after the Elmslie-Trillat procedure for dislocation of the patella.
K Nakagawa; Y Wada; M Minamide; A Tsuchiya; H Moriya
The Journal of bone and joint surgery. British volume, Aug. 2002
We examined 39 patients (45 knees) who had undergone an Elmslie-Trillat procedure for recurrent or habitual dislocation of the patella with a follow-up of more than ten years. The mean age at the time of surgery was 18.4 years; the mean follow-up was 161 months (120 to 238). Using Fulkerson's functional knee score, 41 knees (91%) had an excellent or good result at a mean follow-up of 45 months, and this was maintained in 29 (64%) at the final review. The main cause of deterioration in the clinical results was the onset or worsening of patellofemoral joint pain, not patellar instability.