Shigeru Nagakawa
| Faculty of Health and Medical Science,Department of Medical Course,Sports Science Course | Professor |
| Graduate School of Health Sciences,Doctoral Program in Health Sciences | Professor |
| Graduate School of Health Sciences,Master's and Doctoral Programs in Paramedic | Professor |
Last Updated :2025/10/07
■Researcher basic information
Field Of Study
■Career
Career
- Apr. 2023 - Present
Teikyo Heisei University, Faculty of Health and Medical Science, Department of Medical Course, Professor - Apr. 2016 - Mar. 2023
Teikyo University, School of Medicine - Apr. 2007 - Mar. 2016
Teikyo University, School of Medicine - Apr. 1992 - Mar. 2007
Teikyo University, School of Medicine
■Research activity information
Paper
- Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli
Nami Hatayama; Yoshinori Sato; Rika Tahira; Toki Hori; Shigeru Tansho-Nagakawa; Yasuo Ono; Yusuke Yoshino
BMC Microbiology, 02 Jul. 2025, [Reviewed] - Genetic relatedness of third-generation cephalosporin-resistant Escherichia coli among livestock, farmers, and patients in Japan
Ryuichi Nakano; Akiyo Nakano; Ryuji Nishisouzu; Kenji Hikosaka; Yuki Suzuki; Go Kamoshida; Shigeru Tansho-Nagakawa; Shiro Endo; Kei Kasahara; Yasuo Ono; Hisakazu Yano
One Health, Jun. 2023, [Reviewed]
OBJECTIVES: The third-generation cephalosporin (3GC)-resistant E. coli strains have been detected worldwide in humans and animals. Hence, in this study, we evaluated the prevalence and genetic characteristics of 3GC-resistant E. coli in livestock, farmers, and patients to further analyse if livestock serves as a potential reservoir of antimicrobial-resistant bacteria. METHODS: Faecal samples were collected from 330 healthy livestock (216 cattle and 114 swine), 61 healthy livestock farmers (52 cattle farmers and 9 swine farmers), and 68 non-duplicate 3GC-resistant E. coli isolates were also obtained from the clinical specimens of patients in Japan between 2013 and 2015. Genes associated with resistance in 3GC-resistant E. coli were identified using polymerase chain reaction (PCR) and DNA sequencing. Genotypic diversity was determined by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). RESULTS: We obtained 39 and 17 non-duplicated 3GC-resistant E. coli strains from healthy livestock (33 cattle and six swine) and livestock farmers, respectively. All isolates carried either CTX-M-type extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase genes, with CTX-M-14 being the most frequent. CTX-M producers from livestock and patients belonged to 22 and 19 different sequence types (STs), respectively, and only three STs were the same. Among the 3GC-resistant E. coli from livestock and farmers, three types of CTX-M producers have shown similar characteristics (CTX-M genotype, ST, PFGE patterns, and antimicrobial susceptibilities) and were identified as clonal isolates shared among their farms. CONCLUSIONS: Our study findings indicate that CTX-M-14 is predominant in Japan. No distinct relationship was observed between the 3GC-resistant E. coli isolated from livestock and patients; however, some clonal relatedness was observed between the isolates from livestock and farmers due to their close contact. - Tigecycline Suppresses the Virulence Factors of Multidrug-Resistant Acinetobacter baumannii Allowing Human Neutrophils to Act.
Yoshinori Sato; Nami Hatayama; Tsuneyuki Ubagai; Shigeru Tansho-Nagakawa; Yasuo Ono; Yusuke Yoshino
Infection and drug resistance, 2022, [Reviewed]
Purpose: To determine the ability of human neutrophils to kill multidrug-resistant Acinetobacter baumannii (MDRAB) in the presence of tigecycline (TGC). Methods: Clinical isolates of MDRAB were cultured with human neutrophils and H2O2 in the presence of TGC. The numbers of viable bacteria, catalase activity, gene expression at the K locus of the MDRAB, reactive oxygen species (ROS) production, and granule exocytosis in human neutrophils were determined. Results: There was a time-dependent increase in the numbers of MDRAB after co-culturing with human neutrophils, whereas there was a significant decrease in the MDRAB numbers when co-cultured with both, human neutrophils and TGC for 6 h. The presence or absence of TGC did not affect total ROS production or the expression of CD11b, CD15, and CD63 on human neutrophils occurred when co-cultured with MDRAB. TGC significantly suppressed catalase activity and gene expression at the K locus of MDRAB, and significantly reduced the thickness of the capsule. Additionally, the bacterial viability of TGC-treated MDRAB cultured with H2O2 was lower than that without H2O2 after 6 h of culture. Conclusion: TGC significantly suppressed the expression of catalase and the capsule in MDRAB without adverse effects on neutrophil function, allowing human neutrophils to kill MDRAB. TGC is an effective antibiotic for treating MDRAB infections. - Histopathological Analysis of Acinetobacter baumannii Lung Infection in a Mouse Model
SHIGERU TANSHO-NAGAKAWA; YOSHINORI SATO; TSUNEYUKI UBAGAI; TAKANE KIKUCHI-UEDA; GO KAMOSHIDA; SATOSHI NISHIDA; YASUO ONO
Polish Journal of Microbiology, 01 Dec. 2021, [Reviewed]
Abstract
Acinetobacter baumannii is the main causative pathogen of nosocomial infections that causes severe infections in the lungs. In this study, we analyzed the histopathological characteristics of lung infection with two strains of A. baumannii (ATCC 19606 and the clinical isolate TK1090) and Pseudomonas aeruginosa PAO-1 in C3H/HeN mice to evaluate the virulence of A. baumannii. Survival was evaluated over 14 days. At 1, 2, 5, or 14 days postinfection, mice of C3H/HeN were sacrificed, and histopathological analysis of lung specimens was also performed. Histopathological changes and accumulation of neutrophils and macrophages in the lungs after infection with A. baumannii and P. aeruginosa were analyzed. Following intratracheal inoculation, the lethality of ATCC 19606- and TK1090-infected mice was lower than that of PAO-1-infected mice. However, when mice were inoculated with a sub-lethal dose of A. baumannii, the lung bacterial burden remained in the mice until 14 days post-infection. Additionally, histopathological analysis revealed that macrophages infiltrated the lung foci of ATCC 19606-, TK1090-, and PAO-1-infected mice. Although neutrophils infiltrated the lung foci of ATCC 19606- and TK1090-infected mice, they poorly infiltrated the lung foci of PAO-1-infected mice. Accumulation of these cells in the lung foci of ATCC 19606- and TK1090-infected mice, but not PAO-1-infected mice, was observed for 14 days post-infection. These results suggest that A. baumannii is not completely eliminated despite the infiltration of immune cells in the lungs and that inflammation lasts for prolonged periods in the lungs. Further studies are required to understand the mechanism of A. baumannii infection, and novel drugs and vaccines should be developed to prevent A. baumannii infection. - Effects of colistin and tigecycline on multidrug-resistant Acinetobacter baumannii biofilms: advantages and disadvantages of their combination
Yoshinori Sato; Tsuneyuki Ubagai; Shigeru Tansho-Nagakawa; Yusuke Yoshino; Yasuo Ono
Scientific Reports, Dec. 2021, [Reviewed]Abstract We investigated the antimicrobial effects of colistin (CST) and tigecycline (TGC), either alone or in combination, on biofilm-dispersed and biofilm-embedded multidrug-resistantAcinetobacter baumannii (MDRAB) strains R1 and R2. The bacterial growth of biofilm-dispersed MDRAB was inhibited by CST or TGC. However, the inhibitory effects were attenuated by a combination of CST and low concentrations of TGC. The bactericidal effects of CST, but not TGC, were observed on biofilm-dispersed MDRAB. Notably, the bactericidal effects increased with a combination of CST and high concentrations of TGC, whereas they were attenuated with the combination of CST and low concentrations of TGC. Although biofilm formation by MDRAB decreased with increasing concentrations of CST or TGC, there was no complete disruption of the biofilms. Additionally, the biofilms increased with a combination of 1–2 μg/mL CST and TGC at 2 μg/mL and 2–4 μg/mL for strains R1 and R2, respectively. Biofilm-embedded MDRAB was eradicated with CST, but not TGC. Notably, the eradication effects increased with a combination of CST and high concentrations of TGC, whereas attenuation happened with the combination of CST and low concentrations of TGC. These results provide information on the combined effects of CST and TGC in the treatment of biofilm-associated MDRAB infection. - Analysis of Immune Responses in Acinetobacter baumannii-Infected Klotho Knockout Mice: A Mouse Model of Acinetobacter baumannii Infection in Aged Hosts.
Yoshinori Sato; Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Yasuo Ono
Frontiers in immunology, 2020, [Reviewed]
Acinetobacter baumannii is an important opportunistic pathogen that primarily afflicts elderly people. To clarify the pathogenicity of A. baumannii in the elderly, we investigated immune responses to A. baumannii ATCC 19606 infection in klotho knockout (KO) mice, the mouse model of aging. Following intravenous inoculation, the mice seldom displayed severe symptoms. However, the survival rate was 56% at 7 days post-infection. Bacteria were detected in the lungs of klotho KO mice but not klotho wildtype (WT) mice at 7 days post-infection. Neutrophils, eosinophils, interstitial macrophages, and monocyte/dendritic cell subset in the lungs of klotho KO mice were transiently induced after infection with A. baumannii. The number of alveolar macrophages in klotho KO mice was lower than that in klotho WT mice, except for 1 day post-infection. CD11b expression on neutrophils and alveolar macrophages in the lungs of klotho KO mice was seldom upregulated by the infection. These results suggested that immune functions eliminating bacteria in the lungs of klotho KO mice were insufficient. CD11blow conventional DC cells hardly increased in klotho KO mice infected with A. baumannii. Additionally, the production of interleukin (IL)-10 in the sera of klotho KO mice was significantly higher than that in klotho WT mice, whereas that production of interferon-gamma was not detected in the sera of klotho KO mice. These results suggested that acquired immune responses were hardly induced in klotho KO mice. IL-1β, CXCL1, CXCL2, and CCL2 expression was significantly higher in the lungs of klotho KO mice infected with A. baumannii than in those of klotho WT mice at 1 day post-infection. These results suggested that pulmonary inflammation was elicited in klotho KO mice during early infection. The expression levels of proinflammatory cytokines significantly correlated with TLR9 expression in the lungs of klotho KO mice. The collective results demonstrate an A. baumannii infection state in aged hosts and suggest that pulmonary inflammation and bacterial burden should be noted in aged hosts even in the absence of severe symptoms of A. baumannii infection. - Lipopolysaccharide-Deficient Acinetobacter baumannii Due to Colistin Resistance Is Killed by Neutrophil-Produced Lysozyme.
Go Kamoshida; Takuya Akaji; Norihiko Takemoto; Yusuke Suzuki; Yoshinori Sato; Daichi Kai; Taishi Hibino; Daiki Yamaguchi; Takane Kikuchi-Ueda; Satoshi Nishida; Yuka Unno; Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Tohru Miyoshi-Akiyama; Masataka Oda; Yasuo Ono
Frontiers in microbiology, 2020, [Reviewed]
Acinetobacter baumannii causes nosocomial infections due to its multidrug resistance and high environmental adaptability. Colistin is a polypeptide antibacterial agent that targets lipopolysaccharide (LPS) and is currently used to control serious multidrug-resistant Gram-negative bacterial infections, including those caused by A. baumannii. However, A. baumannii may acquire colistin resistance by losing their LPS. In mouse models, LPS-deficient A. baumannii have attenuated virulence. Nevertheless, the mechanism through which the pathogen is cleared by host immune cells is unknown. Here, we established colistin-resistant A. baumannii strains and analyzed possible mechanisms through which they are cleared by neutrophils. Colistin-resistant, LPS-deficient strains harbor mutations or insertion sequence (IS) in lpx genes, and introduction of intact lpx genes restored LPS deficiency. Analysis of interactions between these strains and neutrophils revealed that compared with wild type, LPS-deficient A. baumannii only weakly stimulated neutrophils, with consequent reduced levels of reactive oxygen species (ROS) and inflammatory cytokine production. Nonetheless, neutrophils preferentially killed LPS-deficient A. baumannii compared to wild-type strains. Moreover, LPS-deficient A. baumannii strains presented with increased sensitivities to antibacterial lysozyme and lactoferrin. We revealed that neutrophil-secreted lysozyme was the antimicrobial factor during clearance of LPS-deficient A. baumannii strains. These findings may inform the development of targeted therapeutics aimed to treat multidrug-resistant infections in immunocompromised patients who are unable to mount an appropriate cell-mediated immune response. - レゾルビンE1はfMLFが誘導するヒト好中球の活性酸素産生を増強する
Mar. 2019, [Reviewed] - Mycobacterium heckeshornense-induced deep abscess in the gluteus maximus: a case report and review of the literature
Hirotoshi Kikuchi; Kurumi Asako; Hajime Kono; Miwa Asahara; Takashi Tanaka; Go Kamoshida; Takane Ueda; Shigeru Nagakawa; Tsuneyuki Ubagai; Yuko Kazumi; Yasuo Ono
The Japanese Journal of Antibiotics, Mar. 2019, [Reviewed] - Resolvin E1, but not resolvins E2 and E3, promotes fMLF-induced ROS generation in human neutrophils.
Yuka Unno; Yoshinori Sato; Hayato Fukuda; Kohei Ishimura; Hiroyuki Ikeda; Mizuki Watanabe; Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Satoshi Shuto; Yasuo Ono
FEBS letters, Aug. 2018, [Reviewed] - Pathogenic bacterium Acinetobacter baumannii inhibits the formation of neutrophil extracellular traps by suppressing neutrophil adhesion.
Kamoshida G; Kikuchi-Ueda T; Nishida S; Tansho-Nagakawa S; Kikuchi H; Ubagai T; Ono Y
Frontiers in Immunology, Feb. 2018, [Reviewed]
Hospital-acquired infections caused by Acinetobacter baumannii have become problematic because of high rates of drug resistance. A. baumannii is usually harmless, but it may cause infectious diseases in an immunocompromised host. Although neutrophils are the key players of the initial immune response against bacterial infection, their interactions with A. baumannii remain largely unknown. A new biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. NETs play a critical role in bacterial killing by bacterial trapping and inactivation. Many pathogenic bacteria have been reported to induce NET formation, while an inhibitory effect on NET formation is rarely reported. In the present study, to assess the inhibition of NET formation by A. baumannii, bacteria and human neutrophils were cocultured in the presence of phorbol 12-myristate 13-acetate (PMA), and NET formation was evaluated. NETs were rarely observed during the coculture despite neutrophil PMA stimulation. Furthermore, A. baumannii prolonged the lifespan of neutrophils by inhibiting NET formation. The inhibition of NET formation by other bacteria was also investigated. The inhibitory effect was only apparent with live A. baumannii cells. Finally, to elucidate the mechanism of this inhibition, neutrophil adhesion was examined. A. baumannii suppressed the adhesion ability of neutrophils, thereby inhibiting PMA-induced NET formation. This suppression of cell adhesion was partly due to suppression of the surface expression of CD11a in neutrophils. The current study constitutes the first report on the inhibition of NET formation by a pathogenic bacterium, A. baumannii, and prolonging the neutrophil lifespan. This novel pathogenicity to inhibit NET formation, thereby escaping host immune responses might contribute to a development of new treatment strategies for A. baumannii infections. - Analysis of membrane antigens on neutrophils from patients with pneumonia.
Tansho-Nagakawa S; Ubagai T; Koshibu Y; Kikuchi-Ueda T; Nakano R; Kamoshida G; Kikuchi H; Ikeda H; Uchida Y; Sakamoto T; Ono Y
Int J Immunol Immunother, 2018, [Reviewed] - Spontaneous formation of neutrophil extracellular traps in serum-free culture conditions
Go Kamoshida; Takane Kikuchi-Ueda; Satoshi Nishida; Shigeru Tansho-Nagakawa; Hirotoshi Kikuchi; Tsuneyuki Ubagai; Yasuo Ono
FEBS OPEN BIO, Jun. 2017, [Reviewed] - The TNF-alpha of mast cells induces pro-inflammatory responses during infection with Acinetobacter baumannii. Immunobiology
Takane Kikuchi-Ueda
Immunobiology, May 2017, [Reviewed]
Mast cells serve important roles as sentinels against bacterial infection by secreting mediators stored in granules. Much of their effectiveness depends upon recruiting and/or modulating other immune cells. The location of mast cells implies that they recognize pathogens invading tissues or mucosal tissues. Acinetobacter baumannii is a gram-negative bacterium that is considered an emerging nosocomial pathogen and causes a wide range of infections associated with high morbidity and mortality. To date, the interaction of A. baumannii with mast cells remains unclear. In this study, we demonstrated an interaction between human LAD2 mast cells and A. baumannii in vitro. When LAD2 cells were co-cultured with live A. baumannii or Pseudomonas aeruginosa PAO1 in vitro for 4h, TNF-α and IL-8 were produced in the culture supernatant. These inflammatory cytokines were not detected in the supernatant after the cells were treated with live bacteria without serum. Gene expression analysis showed that TNF-α and IL-8 mRNA expression increased in A. baumannii- and P. aeruginosa-infected LAD2 cells. Scanning electron microscopy showed that A. baumannii was tightly attached to the surface of LAD2 cells and suggested that A. baumannii may bind to FcγRII (CD32) on LAD2 cells. TNF-α in the culture supernatant from A. baumannii-infected LAD2 cells, showed that PMN activation and migration increased in Boyden chamber assays. These results suggest that mast cells recognize and initiate immune responses toward A. baumannii by releasing the preformed mediator TNF-α to activate effector neutrophils. - Inhibition of Neutrophil Adhesion and Antimicrobial Activity by Diluted Hydrosol Prepared from Rosa damascena
Naho Maruyama; Shigeru Tansho-Nagakawa; Chizuru Miyazaki; Kazuyuki Shimomura; Yasuo Ono; Shigeru Abe
BIOLOGICAL & PHARMACEUTICAL BULLETIN, Feb. 2017, [Reviewed] - A novel bacterial transport mechanism of Acinetobacter baumannii via activated human neutrophils through interleukin-8.
Takane Kikuchi-Ueda
Journal of Leukocyte Biology, Dec. 2016, [Reviewed]
Hospital-acquired infections as a result of Acinetobacter baumannii have become problematic because of high rates of drug resistance. Although neutrophils play a critical role in early protection against bacterial infection, their interactions with A. baumannii remain largely unknown. To elucidate the interactions between A. baumannii and human neutrophils, we cocultured these cells and analyzed them by microscopy and flow cytometry. We found that A. baumannii adhered to neutrophils. We next examined neutrophil and A. baumannii infiltration into Matrigel basement membranes by an in vitro transmigration assay. Neutrophils were activated by A. baumannii, and invasion was enhanced. More interestingly, A. baumannii was transported together by infiltrating neutrophils. Furthermore, we observed by live cell imaging that A. baumannii and neutrophils moved together. In addition, A. baumannii-activated neutrophils showed increased IL-8 production. The transport of A. baumannii was suppressed by inhibiting neutrophil infiltration by blocking the effect of IL-8. A. baumannii appears to use neutrophils for transport by activating these cells via IL-8. In this study, we revealed a novel bacterial transport mechanism that A. baumannii exploits human neutrophils by adhering to and inducing IL-8 release for bacterial portage. This mechanism might be a new treatment target. - Loop-mediated isothermal amplification: Rapid and sensitive detection of the antibiotic resistance gene ISAba1-blaOXA-51-like in Acinetobacter baumannii.
Mu X; Nakano R; Nakano A; Ubagai T; Kikuchi-Ueda T; Tansho-Nagakawa S; Kikuchi H; Kamoshida G; Endo S; Yano H; Ono Y
Journal of microbiological methods, Feb. 2016, [Reviewed]
Carbapenem-resistant Acinetobacter baumannii, which are mainly induced by the production of OXA-type β-lactamases, are among the leading causes of nosocomial infections worldwide. Among the β-lactamase genes, the presence of the OXA-51-like gene carrying the upstream insertion sequence, ISAba1, was found to be one of the most prevalent carbapenem resistance mechanisms utilized by these bacteria. Consequently, it is necessary to develop a rapid detection method for ISAba1-blaOXA-51-like sequence for the timely and appropriate antibiotic treatment of A. baumannii infection. In this study, a loop-mediated isothermal amplification (LAMP) assay was optimized for ISAba1-blaOXA-51-like detection. The LAMP primer set was designed to recognize distinct sequences in the ISAba1-blaOXA-51-like gene and could amplify the gene within 25 min at an isothermal temperature of 60°C. This LAMP assay was able to detect the ISAba1-blaOXA-51-like gene with high specificity; in addition, no cross-reactivity was observed for other types of β-lactamase producers (OXA-23-like, OXA-40-like, OXA-58-like, and IMP-1), as indicated by the absence of false positive or false negative results. The detection limit for this assay was found to be 10(0)CFU per tube which was 100-fold more sensitive than a polymerase chain reaction assay for ISAba1-blaOXA-51-like detection. Furthermore, the LAMP assay provided swift detection of the ISAba1-blaOXA-51-like gene, even directly from clinical specimens. In summary, we have described a new, rapid assay for the detection of the ISAba1-blaOXA-51-like gene from A. baumannii that could be useful in a clinical setting. This method might facilitate epidemiological studies and allow monitoring of the emergence of drug resistant strains. - Rapid detection of the Klebsiella pneumoniae carbapenemase (KPC) gene by loop-mediated isothermal amplification (LAMP)
Ryuichi Nakano; Akiyo Nakano; Yoshikazu Ishii; Tsuneyuki Ubagai; Takane Kikuchi-Ueda; Hirotoshi Kikuchi; Shigeru Tansho-Nagakawa; Go Kamoshida; Xiaoqin Mu; Yasuo Ono
JOURNAL OF INFECTION AND CHEMOTHERAPY, Mar. 2015, [Reviewed] - Acinetobacter baumannii escape from neutrophil extracellular traps (NETs)
Go Kamoshida; Takane Kikuchi-Ueda; Shigeru Tansho-Nagakawa; Ryuichi Nakano; Akiyo Nakano; Hirotoshi Kikuchi; Tsuneyuki Ubagai; Yasuo Ono
JOURNAL OF INFECTION AND CHEMOTHERAPY, Jan. 2015, [Reviewed] - Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae
Ryuichi Nakano; Ryoichi Okamoto; Akiyo Nakano; Noriyuki Nagano; Michiko Abe; Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Takane Kikuchi-Ueda; Osamu Koshio; Hirotoshi Kikuchi; Yasuo Ono
Journal of Microbiological Methods, Sep. 2013, [Reviewed] - Effects of antibiotics in immunomodulatory gene expression of LPS-stimulated human polymorphonuclear leukocytes
T. Ubagai; S. Nagakawa; T. Ueda; R. Nakano; H. Kikuchi; Y. Ono
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, Jun. 2013 - Effects of Erythromycin and Rifampicin on Immunomodulatory Gene Expression and Cellular Function in Human Polymorphonuclear Leukocytes
Xiaoqin Mu; Tsuneyuki Ubagai; Takane Kikuchi-Ueda; Shigeru Tansho-Nagakawa; Ryuichi Nakano; Hirotoshi Kikuchi; Yasuo Ono
CHEMOTHERAPY, 2013, [Reviewed] - Analysis of membrane antigens on neutrophils from patients with sepsis
Shigeru Tansho-Nagakawa; Tsuneyuki Ubagai; Takane Kikuchi-Ueda; Osamu Koshio; Yoji Koshibu; Hirotoshi Kikuchi; Yasuo Ono
JOURNAL OF INFECTION AND CHEMOTHERAPY, Oct. 2012, [Reviewed] - Evaluation of TREM1 Gene Expression in Circulating Polymorphonuclear Leukocytes and Its Inverse Correlation with the Severity of Pathophysiological Conditions in Patients with Acute Bacterial Infections
Tsuneyuki Ubagai; Shigeru Tansho; Ryuji Ieki; Yasuo Ono
JAPANESE JOURNAL OF INFECTIOUS DISEASES, Sep. 2012, [Reviewed] - Enhancement of interleukin-8-induced chemotactic response and reactive oxygen species production in HL-60 cells expressing CXCR1
Takane Kikuchi-Ueda; Shigeru Tansho; Yasuo Ono
JOURNAL OF INFECTION AND CHEMOTHERAPY, Jun. 2012, [Reviewed] - Enhancement of interleukin-8-induced chemotactic response and reactive oxygen species production in HL-60 cells expressing CXCR1.
Takane Kikuchi-Ueda; Shigeru Tansho; Yasuo Ono
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, Jun. 2012
Neutrophils play a pivotal role in immunity against infection by ingesting and killing invading microbes. Neutrophils isolated from human peripheral blood have been used for a number of studies conducted for evaluation of immunomodulating drugs, cytokines, and microbe products. Human promyelocytic leukemia cells, HL-60, have been extensively studied because they can differentiate into neutrophil-like cells by addition of all-trans retinoic acid or dimethyl sulfoxide. For a system that would always allow experimental use of granulocytic cells in a uniformly activated state, we have established HL-60 cell lines with increased migratory activity by transducing the CXC chemokine receptor 1 (CXCR1) gene. When these cell lines were primed with CXC chemokine ligand 8 (IL-8), a slight increase in reactive oxygen species production induced by phorbol myristate acetate (PMA) or zymosan A stimuli was observed. A significance increase in migratory activity was noticed when the HL-60 cells transduced CXCR1 were stimulated with IL-8 in the Boyden chamber method. The gene-transduced HL-60 cell lines may be used as a substitute for neutrophils in screening the effects of various immunomodulating drugs on the migratory activity induced by IL-8. - A d-octapeptide drug efflux pump inhibitor acts synergistically with azoles in a murine oral candidiasis infection model
Kazumi Hayama; Hiroko Ishibashi; Sanae A. Ishijima; Kyoko Niimi; Shigeru Tansho; Yasuo Ono; Brian C. Monk; Ann R. Holmes; David R. K. Harding; Richard D. Cannon; Shigeru Abe
FEMS MICROBIOLOGY LETTERS, Mar. 2012, [Reviewed] - A D-octapeptide drug efflux pump inhibitor acts synergistically with azoles in a murine oral candidiasis infection model.
Kazumi Hayama; Hiroko Ishibashi; Sanae A Ishijima; Kyoko Niimi; Shigeru Tansho; Yasuo Ono; Brian C Monk; Ann R Holmes; David R K Harding; Richard D Cannon; Shigeru Abe
FEMS microbiology letters, Mar. 2012
Clinical management of patients undergoing treatment of oropharyngeal candidiasis with azole antifungals can be impaired by azole resistance. High-level azole resistance is often caused by the overexpression of Candida albicans efflux pump Cdr1p. Inhibition of this pump therefore represents a target for combination therapies that reverse azole resistance. We assessed the therapeutic potential of the D-octapeptide derivative RC21v3, a Cdr1p inhibitor, in the treatment of murine oral candidiasis caused by either the azole-resistant C. albicans clinical isolate MML611 or its azole-susceptible parental strain MML610. RC21v3, fluconazole (FLC), or a combination of both drugs were administered orally to immunosuppressed ICR mice at 3, 24, and 27 h after oral inoculation with C. albicans. FLC protected the mice inoculated with MML610 from oral candidiasis, but was only partially effective in MML611-infected mice. The co-application of RC21v3 (0.02 μmol per dose) potentiated the therapeutic performance of FLC for mice infected with either strain. It caused a statistically significant decrease in C. albicans cfu isolated from the oral cavity of the infected mice and reduced oral lesions. RC21v3 also enhanced the therapeutic activity of itraconazole against MML611 infection. These results indicate that RC21v3 in combination with azoles has potential as a therapy against azole-resistant oral candidiasis. - [Effect of oral administration of β-D-glucan from Aureobasidium pullulans ADK-34 on Candida and MRSA infections in immunosuppressed mice].
Tanioka A; Hayama K; Mitsuya M; Tansho S; Ono Y; Tsubaki K; Abe S
Medical mycology journal, 2012, [Reviewed]
We examined the effect of the oral administration of β-D-glucan derived from Aureobasidium pullulans ADK-34 (AP-FBG) on Candida albicans or methicillin-resistant Staphylococcus aureus (MRSA) infection in immunosuppressed mice. Mice pretreated with cyclophosphamide (CY) were intraperitoneally administered AP-FBG for 4 days and then infected with 6×104 C. albicans cells. In a preliminary experiment, the survival time of the Candida-infected mice treated with AP-FBG was clearly prolonged. Similarly, the effect of the oral administration of AP-FBG was examined. Mice were orally given 2.5% AP-FBG in feed for 42 days from 14 days prior to 2×104 C. albicans cells infection. The survival time of mice treated with AP-FBG was significantly prolonged and the viable cell count in the kidneys of the survivors was significantly decreased at 30 days after infection. The effects of the oral administration of AP-FBG on intestinal MRSA infection were also examined. Mice were given 2.5% AP-FBG orally in feed for 30 days before and after oral MRSA infection and treated with CY 12 days after the infection. The number of viable MRSA cells or the IgA production in feces did not significantly change, while AP-FBG administration seemed to relieve temporally the loss of body weight of mice. Conclusions: These results suggest that oral pre-administration of AP-FBG promoted resistance of CY-treated mice to C. albicans and lessened the weight reduction of CY-mice infected by MRSA. - Recurrent Helicobacter cinaedi Cellulitis and Bacteremia in a Patient with Systemic Lupus Erythematosus
Hirotoshi Kikuchi; Kurumi Asako; Shigeru Tansho; Takane Ueda; Osamu Koshio; Tuneyuki Ubagai; Miwa Asahara; Sayoko Kawakami; Yasuo Ono
INTERNAL MEDICINE, 2012, [Reviewed] - Evaluation of TREM1 gene expression in circulating polymorphonuclear leukocytes and its inverse correlation with the severity of pathophysiological conditions in patients with acute bacterial infections.
Tsuneyuki Ubagai; Shigeru Tansho; Ryuji Ieki; Yasuo Ono
Japanese journal of infectious diseases, 2012
During bacterial infection, activated polymorphonuclear leukocytes (PMNs) often cause inflammation and organ dysfunction in severely ill patients. Gene expression was analyzed in circulating PMNs isolated from these patients to determine the distinct expression profile. We focused on immunomodulatory genes, such as those for pattern recognition receptors, inflammatory cytokines, PMN surface antigens, and myeloid cell receptors in PMNs. Gene expression in 23 patients (12 with pneumonia and 11 with sepsis) were analyzed using quantitative real-time polymerase chain reaction. The mRNA levels of TLR2 (20/23 cases) and CD14 (18/23 cases) were upregulated in the PMNs of patients when compared with healthy subjects. The mRNA expression levels of TLR4 (16/23 cases) and IL6 (16/23 cases) were downregulated in patients' PMNs, and of TNFA (16/23 cases) were upregulated in these cells. Although mRNA levels of IL8RA (15/23 cases) were downregulated in PMNs, MAC-1 mRNA levels (14/23 cases) were upregulated in the same cells. Copies of the TREM1 transcript were 0.7- to 2.1-fold higher in patients with moderate pneumonia than in the healthy subjects; the average fold change was 1.1. The mRNA levels were 0.3-fold lower in the patients with severe pneumonia and sepsis than in the healthy subjects. In conclusion, the downregulation of TREM1 expression in PMNs is associated with the severity of the pathophysiological conditions and may be used as a surrogate marker of acute bacterial infections. - Recurrent Helicobacter cinaedi cellulitis and bacteremia in a patient with systemic lupus erythematosus.
Hirotoshi Kikuchi; Kurumi Asako; Shigeru Tansho; Takane Ueda; Osamu Koshio; Tuneyuki Ubagai; Miwa Asahara; Sayoko Kawakami; Yasuo Ono
Internal medicine (Tokyo, Japan), 2012
A 31-year-old woman who had developed systemic lupus erythematosus at 17 years of age was admitted to the hospital for suspected cellulitis in the lower extremities. A blood culture performed upon admission to the hospital detected Helicobacter cinaedi (H. cinaedi), which was also isolated in blood and fecal cultures obtained on the 42nd hospital day. Bacterial translocation of H. cinaedi present in the intestines may have led to the development of recurrent bacteremia and cellulitis. In cases such as this, appropriate antibiotics therapy might be needed for more than one month. Moreover, H. cinaedi, a cause of emerging infections, requires a long period of time to grow; therefore it is important to extend the culture duration when the presence of this bacterium is suspected. - Condition for effective inhibition of Candida albicans growth by lactoferricin B and its therapeutic activity with fluconazole against oral candidiasis in mice
Takashi Tanaka; Takafumi Okutomi; Hiroyuki Wakabayashi; Hiroko Ishibashi; Shigeru Tansho; Kentaro Ninomiya; Hideyo Yamaguchi; Yasuo Ono; Shigeru Abe
Medical mycology research, 2011 - Suppression of phosphorylation of extracellular-signal-regulated kinase and p38 mitogen-activated protein kinase in polymorphonuclear leukocytes by the proton pump inhibitor lansoprazole
Osamu Koshio; Shigeru Tansho; Tsuneyuki Ubagai; Toshio Nakaki; Yasuo Ono
JOURNAL OF INFECTION AND CHEMOTHERAPY, Apr. 2010, [Reviewed] - Suppression of phosphorylation of extracellular-signal-regulated kinase and p38 mitogen-activated protein kinase in polymorphonuclear leukocytes by the proton pump inhibitor lansoprazole.
Osamu Koshio; Shigeru Tansho; Tsuneyuki Ubagai; Toshio Nakaki; Yasuo Ono
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, Apr. 2010
Lansoprazole (LPZ) is a proton pump inhibitor that suppresses gastric secretion and exerts anti-inflammatory effects on immune cells. Recently, LPZ has been used for the treatment of peptic ulcer and gastritis, which can be caused by Helicobacter pylori, due to its potent acid-suppressive effects. We focused the aim to the anti-inflammatory effects on the over-activation of neutrophils, and investigated the effects of LPZ on the signal transduction of the mitogen-activated protein kinase (MAPK) family. LPZ slightly phosphorylated p38 MAPK of neutrophils at a concentration of 10 microg/ml , but did not phosphorylate extracellular-signal regulated kinase (ERK) 1/2. Pretreatment of neutrophils with (1-5 microg/ml ) LPZ strongly attenuated the phorbol-12-myristate-13-acetate-stimulated phosphorylation of ERK1/2, and LPZ slightly suppressed the lipopolysaccharide (LPS)- and N-formylmethionylleucylphenylalanine-stimulated phosphorylation of p38. ERK1/2 produces the mitochondrial anti-apoptotic proteins, and the signaling pathway from LPS and N-formylmethionylleucylphenylalanine to p38 is the main pathway for reactive oxygen species production. The mechanism of anti-inflammatory effect of LPZ on hyper-activated neutrophils is suggested to be the suppression of signal transduction of ERK1/2 and p38 MAPK. - Influences of aflatoxin B-1 on reactive oxygen species generation and chemotaxis of human polymorphonuclear leukocytes
Tsuneyuki Ubagai; Shigeru Tansho; Tadashi Ito; Yasuo Ono
TOXICOLOGY IN VITRO, Jun. 2008, [Reviewed] - Influences of aflatoxin B1 on reactive oxygen species generation and chemotaxis of human polymorphonuclear leukocytes.
Tsuneyuki Ubagai; Shigeru Tansho; Tadashi Ito; Yasuo Ono
Toxicology in vitro : an international journal published in association with BIBRA, Jun. 2008
The effects of aflatoxin (AF), a hepatotoxic agent and the strongest carcinogen in nature, on polymorphonuclear leukocyte (PMN) chemotaxis and chemiluminescence (CL) were studied. Luminol-dependent CL activity, which reflects the production of reactive oxygen species (ROS) from PMNs, was up-regulated to approximately 150% when PMNs were treated with 0.05 ng/ml of AFB1 upon stimulation with N-formyl-methionine-leucine-phenylalanine (fMLP) or zymosan. On the other hand, PMN CL activity was down-regulated to approximately 25% of the control when PMNs were treated with 50 ng/ml of AFB1 upon stimulation with zymosan. The effect of AFB1 on PMN chemotaxis was also investigated using the Boyden chamber method. The chemotactic ability of PMNs when interleukin (IL)-8 was used as a chemoattractant was inhibited in a dose-dependent manner at AFB1 concentrations of >10 ng/ml. In reverse transcriptase (RT)-PCR analysis, gene expression levels of CXC chemokine receptor (CXCR)1/2, whose ligands are IL-8, granulocyte chemotactic protein (GCP)-2, neutrophil attractant-activation protein (NAP)-2, and epithelial neutrophil-activating protein (ENA)-78, which regulate PMN chemotaxis, were also down-regulated in a dose-dependent manner by 50 ng/ml AFB1. mRNA expression levels of CXCR1/2 were down-regulated to approximately 85% of that in the controls when PMNs were treated with 100 ng/ml AFB1. These results suggest that a high concentration of AFB1 reduces the chemotactic ability of PMNs via the CXCR1/2 cascade indirectly. - [Host defense system against infection].
Ono Y; Tansho S; Koshio O; Ubagai T
Nihon rinsho. Japanese journal of clinical medicine, Feb. 2007, [Reviewed] - Efficacy of intravenous itraconazole against invasive pulmonary aspergillosis in neutropenic mice.
Shigeru Tansho; Shigeru Abe; Hiroko Ishibashi; Shinichi Torii; Hisaya Otani; Yasuo Ono; Hideyo Yamaguchi
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, Dec. 2006
The efficacy of itraconazole (ITZ) solubilized in hydroxypropyl-beta-cyclodextrin (ITZ-IV) was examined in a murine model of invasive pulmonary aspergillosis (IPA). Immunosuppressed mice were infected by the intratracheal inoculation of Aspergillus fumigatus conidia (2 x 10(6) conidia/mouse). Their body weight rapidly decreased and they died within 6 days after infection. Intravenous administration of various doses of ITZ-IV was started 24 h after infection and was continued once a day for 4 days. ITZ-IV at daily doses of 10, 20, or 40 mg/kg was as effective as the intraperitoneal administration of amphotericin B (AMPH) at a dosage of 1 mg/kg daily in improving survival. ITZ-IV (20 mg/kg per day), as well as AMPH (1 mg/kg per day) significantly lowered the fungal burden in the pulmonary tissues. Histological improvement was seen within 2 days after the beginning of administration of ITZ-IV (20 mg/kg per day). In mice intravenously given a single dose of ITZ-IV (20 mg/kg), the blood level and pulmonary tissue level of ITZ plus its active metabolites, mainly hydroxyitraconazole (OH-ITZ), decreased gradually after the injection, but after 4 h their concentration was still between 1.4 microg/ml (ITZ) and 1.9 microg/ml (OH-ITZ), concentrations that were approximately 10 to 20 times greater than the minimum inhibitory concentration (MIC) of ITZ for challenging the strain of A. fumigatus (0.16 microg/ml). These results support the clinical usefulness of ITZ-IV for the treatment of IPA in immunocompromised patients. - Efficacy of intravenous itraconazole against invasive pulmonary aspergillosis in neutropenic mice
Shigeru Tansho; Shigeru Abe; Hiroko Ishibashi; Shinichi Torii; Hisaya Otani; Yasuo Ono; Hideyo Yamaguchi
Journal of Infection and Chemotherapy, 2006, [Reviewed] - [Transferability of vanA gene from vancomycin-resistant Enterococcus faecalis in the digestive tract of specific pathogen-free mice].
Ikeda T; Watanabe T; Matsumoto K; Murayama SY; Koshio O; Tansho S; Ono Y
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Nov. 2004, [Reviewed]
We evaluated the transferability of vanA gene from vancomycin-resistant Enterococcus faecalis (VREF) to vancomycin-sensitive E. faecalis (VSEF) in vitro and in vivo. In vitro conjugal transfer experiment by filter mating, the vanA gene of VREF was transferable at the high frequency to VSEF and a mutant strain which cured vanA gene of VREF. In vivo studies in the digestive tract of specific pathogen-free mice pretreated with oral antibiotics, transconjugants were also detected from the feces of a mouse at the lower frequency. However, the colonization of transconjugants was transient. The vanA gene in the donor and the transconjugant strain was confirmed by using a polymerase chain reaction method.
These results suggest that VSEF colonizing in the human digestive tract might be developed to VREF by transferring of the vanA gene. - Opsonic activity assessment of human intravenous immunoglobulin preparations against drug-resistant bacteria.
Yasuo Ono; Tadashi Ito; Takeshi Watanabe; Osamu Koshio; Shigeru Tansho; Tatsuo Ikeda; Sayoko Kawakami; Yukihisa Miyazawa
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, Aug. 2004
We have used the ability of opsonized bacteria to stimulate luminol-enhanced chemiluminescence (CL) of human polymorphonuclear leukocytes (PMN) to examine the opsonic capabilities of commercially available human intravenous immunoglobulin (i.v.Ig) preparations. The method was tested against 14 strains of drug-resistant gram-positive bacteria (including methicillin-resistant Staphylococcus aureus, hetero-vancomycin-resistant S. aureus, vancomycin-resistant enterococci, penicillin-resistant Streptococcus pneumoniae), and 23 strains of gram-negative bacteria (including extended-spectrum beta-lactamase-producing bacteria, metallo-beta-lactamase-producing bacteria, beta-lactamase-negative ampicillin-resistant Haemophilus influenzae). An Fc-intact i.v.Ig preparation treated with polyethylene glycol (PEG) was evaluated for opsonization effectiveness against these bacteria in vitro. The opsonization of these organisms was enhanced by an Fc-intact i.v.Ig, and the opsonic activity was dose dependent. A pepsin-treated i.v.Ig preparation exhibited poor opsonic activity for all bacteria tested. These results suggest that Fc-intact i.v.Ig, which augments opsonic activity against various drug-resistant bacteria, will be a useful addition to the treatment of severe bacterial infections in immunocompromised patients with impaired serum opsonic capacity. - Production of anti-Candida antibodies in mice with gut colonization of Candida albicans
S Tansho; S Abe; H Ishibashi; M Mitsuya; K Wada; T Ikeda; N Suegara; O Koshio; Y Ono; H Yamaguchi
MEDIATORS OF INFLAMMATION, Jun. 2004, [Reviewed] - Production of anti-Candida antibodies in mice with gut colonization of Candida albicans.
Shigeru Tansho; Shigeru Abe; Hiroko Ishibashi; Masayasu Mitsuya; Kayoko Wada; Tatsuo Ikeda; Nobuo Suegara; Osamu Koshio; Yasuo Ono; Hideyo Yamaguchi
Mediators of inflammation, Jun. 2004
BACKGROUND: Production of antibodies that are specific for allergens is an important pathological process in inflammatory allergic diseases. These contain the antibodies against antigens of Candida albicans, one of the normal microbial flora in an intestinal tract. We studied the effects of the prednisolone administration on the production of anti-Candida antibodies in the gastrointestinally C. albicans-colonized mice. METHODS AND MATERIALS: BALB/c mice, treated with antibacterial antibiotics to decontaminate indigenous intestinal bacterial flora, were inoculated intragastrically with C. albicans. The mice, in which C. albicans grows intestinally, were administered prednisolone to induce temporary immunosuppression. The Candida growth in their intestinal tract and their antibody response to Candida were examined. RESULTS: Antibiotic treatment allowed establishment of C. albicans gastrointestinal colonization, but did not cause subsequent systemic dissemination of C. albicans in all the animals. When these animals received an additional treatment with prednisolone, they showed a significantly higher population of C. albicans in their feces than those of animals treated with antibiotics alone, and the organisms were recovered even from their kidney. This systemic dissemination by C. albicans appeared to be temporal, because all the mice survived without any symptoms for more than 2 months. Examination of the serum titers of total immunoglobulin (Ig)E antibodies and specific IgE and IgG antibodies against Candida antigens demonstrated that titers of total IgE increased, partially by day 14 and clearly at day 27, in prednisolone-treated Candida-colonized mice. Without prednisolone treatment, an increment of the serum titer was scarcely observed. By day 27, corresponding to the increase of total IgE, the anti-Candida IgE and IgG titer increased in mice of the prednisolone-treated group. CONCLUSION: Administration of prednisolone to Candida-colonized mice can induce production of the IgG, IgE antibodies against Candida antigens, perhaps through temporal systemic dissemination of Candida from the intestinal tract. - Opsonic activity assessment of human intravenous immunoglobulin preparations against drug-resistant bacteria
Yasuo Ono; Tadashi Ito; Takeshi Watanabe; Osamu Koshio; Shigeru Tansho; Tatsuo Ikeda; Sayoko Kawakami; Yukihisa Miyazawa
Journal of Infection and Chemotherapy, 2004, [Reviewed] - Protection of mice from lethal endogenous Candida albicans infection by immunization with Candida membrane antigen
S Tansho; S Abe; S Mizutani; Y Ono; K Takesako; H Yamaguchi
MICROBIOLOGY AND IMMUNOLOGY, 2002, [Reviewed] - Acidic polysaccharides from rhizomes of Atractylodes lancea as protective principle in Candida-infected mice
N Inagaki; Y Komatsu; H Sasaki; H Kiyohara; H Yamada; H Ishibashi; H Tansho; H Yamaguchi; S Abe
PLANTA MEDICA, Jul. 2001 - Effective inhibition of Candida albicans growth by the combination of murine peritoneal neutrophils and activated macrophages
Shigeru Tansho; Shigeru Abe; Tohru Tansho; Hideyo Yamaguchi
Microbiology and Immunology, 1999, [Reviewed] - Cooperative Anti-Candida Effects of Lactoferrin or Its Peptides in Combination with Azole Antifungal Agents
WAKABAYASHI Hiroyuki; ABE Shigeru; OKUTOMI Takafumi; TANSHO Shigeru; KAWASE Kouzou; YAMAGUCHI Hideyo
Microbiol.Immunol., 20 Nov. 1996 - A glucocorticoid antagonist, mifepristone affects anti-Candida activity of murine neutrophils in the presence of prednisolone in vitro and experimental candidiasis of prednisolone-treated mice in vivo
Shigeru Abe; Megumi Ohnishi; Takao Nohmi; Masahiro Katoh; Shigeru Tansho; Hideyo Yamaguchi
FEMS Immunology and Medical Microbiology, 1996 - Suppression of Anti-Candida Activity of Murine Neutrophils by Progesterone In Vitro: A Possible Mechanism in Pregnant Women’s Vulnerability to Vaginal Candidiasis
Takao Nohmi; Shigeru Abe; Shigeru Tansho; Hideyo Yamaguchi; Kazuyoshi Dobashi
Microbiology and Immunology, 1995, [Reviewed] - Suppression of Anti-Candida Activity of Murine and Human Neutrophils by Glucocorticoids
Nohmi Takao; Abe Shigeru; Tansho Shigeru; Yamaguchi Hideyo
Japanese Journal of Microbiology, 1994
Effects of glucocorticoid (GC) compounds on inhibitory activity of neutrophils to mycelial growth of Candida albicans were examined by in vitro crystal violet staining method with 14hr coculture. Both GC hormones (hydrocortisone _??_6×10-7M and corticosterone _??_10-6M) and anti-inflammatory GC agents (prednisolone _??_10-7M and dexamethasone _??_10-8M) significantly suppressed anti-Candida activity of murine casein-induced neutrophils. Anti-Candida activity of human neutrophils prepared from peripheral blood was also suppressed by hydrocortisone (_??_6× 10-7M). These GC compounds did not affect the Candida growth in the absence of neutrophils. Steroidal compounds without anti-inflammatory activity, cholesterol, cholic acid, aldosterone did not suppress neutrophil activity. These results suggest that GCs at their physiological or clinical concentration may suppress anti-Candida activity of neutrophils in vivo. - Inhibition of Candida albicans Growth by Murine Peritoneal Neutrophils and Augmentation of the Inhibitory Activity by Bacterial Lipopolysaccharide and Cytokines
Shigeru Tansho; Shigeru Abe; Hideyo Yamaguchi
Microbiology and Immunology, 1994, [Reviewed] - A Rapid Colorimetric Assay for Determination of Leukocyte-Mediated Inhibition of Mycelial Growth of Candida albicans
Shigeru Abe; Tamae Satoh; Yoshiko Tokuda; Shigeru Tansho; Hideyo Yamaguchi
Microbiology and Immunology, 1994, [Reviewed] - Prolongation of rat cardiac allograft survival by a monoclonal antibody: anti-rat intercellular adhesion molecule-1.
H Kobayashi; T Miyano; A Yamataka; T Yamataka; H Yagita; H Eto; Y Ligo; S Tansyo; Y Kojima; T Tamatani
Cardiovascular surgery (London, England), Oct. 1993
A mouse monoclonal antibody 1A29, which binds to the rat intercellular adhesion molecule-1 (ICAM-1), was studied for its effect on cardiac allograft survival. Expression of ICAM-1 was detectable only on vascular endothelium in normal heart, but was induced on myocardium associated with interstitial mononuclear cell infiltration during acute rejection. Treatment with monoclonal antibody 1A29 for 10 days after transplantation in 15 rats significantly prolonged allograft survival (mean(s.d.) 18(2) days; P < 0.001), as compared with 15 isotype-matched control monoclonal antibody (mean(s.d.) 10(1) days) recipients. Five-day treatment with monoclonal antibody 1A29, when started at 5 days after transplantation (the time for which acute rejection is ongoing), also significantly extended the survival (mean(s.d.) 12(1) days; P < 0.01) in seven rats. On histological examination, treatment with monoclonal antibody 1A29 reduced the degree of T-cell infiltration of both CD4+ and CD8+ subsets, and greatly reduced myocardial necrosis, vascular injury and intravenous thrombi. These results indicate that an anti-ICAM-1 monoclonal antibody can be used to prevent or treat acute rejection in the rat cardiac allograft model and suggest that human ICAM-1 is an important target for immunosuppression in clinical organ transplantation. - A novel homodimeric molecule involved in human T cell activation.
T Kobata; H Yagita; H Matsuda; S Tansyo; H Yakura; M Katagiri; K Okumura
Journal of immunology (Baltimore, Md. : 1950), 01 Feb. 1990
A mAb, 10D1, was obtained by fusing spleen cells from BALB/c mice immunized with a CD3/TCR- human T cell line, P12/ichikawa, to mouse myeloma cells, P3X63-Ag8-653. 10D1 mAb is specific for T cells in that it reacted with all the T cell lines tested, but not with B or myeloid cell lines. A small fraction of normal peripheral blood T cells, preferentially CD4+, was also reactive with 10D1 mAb. Biochemical studies revealed that 10D1 mAb recognizes a disulfide-linked homodimeric molecule composed of 90-kDa polypeptide. 10D1 mAb induced a substantial proliferation of peripheral blood T cells when cross-linked with goat anti-mouse Ig antibody. The elimination of CD4+ cells totally abrogated the proliferative response induced by 10D1 mAb, whereas the elimination of CD8+ cells rather enhanced it. The proliferative response of peripheral blood T cells induced by 10D1 mAb was almost completely inhibited after modulation of the CD3/TCR complex with anti-CD3 mAb. In addition, a prompt increase in intracellular [Ca2+] was observed in a CD3+ T cell line, Jurkat but not in its surface CD3- mutant when 10D1 mAb was added. These results indicate that the 10D1 molecule is involved in a novel pathway of human CD4+ T cell activation, which is associated with the CD3/TCR-mediated pathway. - Expression and function of CD2 during murine thymocyte ontogeny.
H Yagita; J Asakawa; S Tansyo; T Nakamura; S Habu; K Okumura
European journal of immunology, Dec. 1989
CD2, originally recognized as the sheep erythrocyte receptor of human T cells, has been implicated in early T cell development in the thymus. In this report, we examined the expression and functional role of CD2 during murine thymocyte ontogeny by using monoclonal antibodies to murine CD2. Surface expression of CD2 was first detected in Thy-1+ fetal thymocytes at day 14 of gestation and it progressively increased during CD4-CD8- phenotype. Surface IL 2 receptor (CD25) expression was readily detected in surface CD2- cells at day 13 of gestation and the majority of CD2+ cells appeared to be generated from CD25+ cells thereafter. In adult CD4-CD8- thymocytes, the expression of CD2 and CD25 was mutually exclusive. These results indicate that surface CD2 expression is not a prerequisite for CD25 induction during murine thymocyte ontogeny. This was further confirmed by fetal thymus organ culture in which anti-murine CD2 mAb was included. The antibody treatment led to a suppressed CD2 expression on thymocytes; however, there was no effect on the appearance of CD25. Moreover, no influence on the development of mature CD3+ thymocytes was observed after fetal thymus organ culture in the presence of anti-murine CD2 mAb, and a substantial number of CD3+CD2- cells was demonstrated in fetal and adult CD4-CD8- thymocytes. These findings argue against the functional relevance of CD2 expression during early T cell development as proposed in humans. - Purification and characterization of a cytolytic protein from purple fluid of the sea hare, Dolabella auricularia
Yamazaki M.; Tansho S.; Kisugi J.; Muramoto K.; Kamiya H.
Chem Pharm Bull, Aug. 1989, [Reviewed] - CD2 expression in murine B cell lineage.
H Yagita; T Nakamura; J Asakawa; H Matsuda; S Tansyo; Y Iigo; K Okumura
International immunology, 1989
CD2 expression in murine B cell lineage was examined by flow cytometric and immunoprecipitation studies with anti-murine CD2 mAb and by Northern blot analysis. Cell surface expression of CD2 was demonstrated on all peripheral B cells and cell lines of B lineage. The murine CD2 transcript of 1.3 kb was detected in these B cells. An identical glycoprotein of 55-67 KD was precipitated from the lysates of surface radioiodinated thymocytes, splenic T and B cells, T and B lymphomas, RL male 1 and BCL-1, with anti-murine CD2 mAb. The majority of bone marrow B cells and a half of pre-B cells were found to be positive for CD2 expression. These results indicate that murine CD2 is expressed on B cell lineage at certain differentiation stages.
MISC
- 学生実習の効率化と実習理解度の変化 コロナ禍を経験して
Jul. 2024
Lead - 臨床分離されたカルバペネム耐性菌におけるコリス チン耐性の解析
Apr. 2023, [Reviewed] - 臨床分離されたStaphylococcus haemolyticusのバイオフィルム形成に及ぼすテイコプラニンの効果
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌が形成するバイオフィルムに対するメロぺネム(MEPM)の有効性評価
Mar. 2023 - 臨床分離されたStaphylococcus haemolyticusのバイオフィルム形成に及ぼすテイコプラニンの効果
Mar. 2023 - 多剤耐性Acinetobacter baumanniiにおけるブレイクポイント・チェッカーボードプレートを用いた薬剤併用効果の検討
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌臨床分離株のバイオフィルム形成能の評価
Mar. 2023 - 分類・疫学・感染症 CRISPR-Cas12aを用いた多剤耐性アシネトバクターのカルバペネマーゼ遺伝子検査
Feb. 2023 - 健常な学生集団から回収されたメチシリン耐性ブドウ球菌の解析
Feb. 2023 - 健常な学生集団から回収されたメチシリン耐性ブドウ球菌の解析
Feb. 2023, [Reviewed] - 分類・疫学・感染症 CRISPR-Cas12aを用いた多剤耐性アシネトバクターのカルバペネマーゼ遺伝子検査
Feb. 2023, [Reviewed] - PCR-based ORF typing of Methicillin-resistant Staphylococcus isolates from medical school students
金子日向子; 西田智; 永川茂; 上田たかね; 佐藤義則; 斧康雄; 斧康雄; 吉野友祐
日本細菌学雑誌(Web), 2023 - 臨床分離されたカルバペネム耐性菌におけるコリスチン耐性の解析
Oct. 2022, [Reviewed] - メタロ-β-ラクタマーゼIMP-1産生Klebsiella michiganensisのプラスミド解析
May 2022 - チゲサイクリン存在下におけるヒト好中球の多剤耐性Acinetobacter baumanniiに対する殺菌効果の解析
Mar. 2022 - 多剤耐性Acinetobacter baumanniiのバイオフィルムに対するコリスチン/チゲサイクリンの併用効果
Sep. 2021 - 高齢者におけるAcinetobacter baumannii感染重症化機序の解明(ヒト早発性老化症候群モデルマウスによる感染免疫応答の解析)
Apr. 2021 - メタロ-β-ラクタマーゼIMP-1産生Klebsiella michiganensisのゲノム解析
Apr. 2021 - Galectin-8のヒト好中球機能に及ぼす影響
Mar. 2020, [Reviewed] - マクロファージの殺菌に対するAcinetobacter baumanniiの抵抗性評価
Mar. 2020, [Reviewed] - 経皮経肝胆道ドレナージ患者におけるOXA-48型カルバペネマーゼ産生E.coliの出現(Emergence of E. coli producing OXA-48-like carbapenemase in a patient with percutaneous transhepatic biliary drainage)
西田 智; 上田 たかね; 祖母井 庸之; 永川 茂; 佐藤 義則; 石垣 しのぶ; 古川 泰司; 斧 康雄; 浅原 美和
感染症学雑誌, Mar. 2020
(一社)日本感染症学会 - ヒト早発性老化症候群モデルマウスを用いたAcinetobacter baumannii感染に対する免疫応答の解析
Mar. 2020 - ヒト好中球機能に及ぼすGalectin-8の影響
Mar. 2020 - 電撃紫斑病患者から分離された肺炎球菌の全ゲノム解析
Mar. 2020 - Galectin-8のヒト好中球機能に及ぼす影響
Mar. 2020 - マクロファージの殺菌に対するAcinetobacter baumanniiの抵抗性評価
Mar. 2020 - 分類・疫学・感染症 汎薬剤耐性菌Klebsiella pneumoniaeシークンエンス11型のゲノムの分析(Taxonomy, Epidemiology, and Infectious Diseases Genomic analysis of a pandrug-resistant Klebsiella pneumoniae sequence type 11)
西田 智; 上田 たかね; 祖母井 庸之; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Jan. 2020
日本細菌学会 - Multidrug-resistant A.baumanniiは活性酸素種に抵抗してマクロファージ内で生存する(Multidrug-resistant A. baumannii resists reactive oxygen species and survives in macrophages)
佐藤 義則; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Jan. 2020
日本細菌学会 - AIPF患者から分離されたStreptococcus pneumoniaeの2株の暫定ゲノム配列(Draft genome sequences of two strains of Streptococcus pneumoniae from AIPF patients)
Jan. 2020 - 分類・疫学・感染症(Taxonomy, Epidemiology, and Infectious Diseases Genomic analysis of a pandrug-resistant Klebsiella pneumoniae sequence type 11)
西田 智; 上田 たかね; 祖母井 庸之; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Jan. 2020
日本細菌学会 - Draft genome sequences of two strains of Streptococcus pneumoniae from AIPF patients(和訳中)
Jan. 2020 - Multidrug-resistant A.baumanniiは活性酸素種に抵抗してマクロファージ内で生存する(Multidrug-resistant A. baumannii resists reactive oxygen species and survives in macrophages)
佐藤 義則; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Jan. 2020
日本細菌学会 - Acinetobacter baumannii臨床分離株の肺感染モデルを用いた病原性解析
Mar. 2019, [Reviewed] - Acinetobacter baumannii感染klothoマウスにおける免疫応答の解析(Analysis of immune responses in klotho mice infected with Acinetobacter baumannii)
佐藤 義則; 永川 茂; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Mar. 2019, [Reviewed]
日本細菌学会 - 高齢宿主を想定したAcinetobacter baumannii感染に対する免疫応答の解析
佐藤義則; 永川茂; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
感染症学雑誌, Mar. 2019 - コリスチン耐性Acinetobacter baumanniiと好中球の相互作用解析
鈴木雄介; 鴨志田剛; 赤路卓哉; 上田たかね; 西田智; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, Mar. 2019 - コリスチン耐性Acinetobacter baumannii菌株の樹立と性状解析
赤路卓哉; 鴨志田剛; 鈴木雄介; 西田智; 上田たかね; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, Mar. 2019 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性解析
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
感染症学雑誌, Mar. 2019 - レゾルビンE1によるヒト好中球の活性酸素産生活性化機構の解明(Resolvin E1 enhances fMLF-induced ROS generation via LTB4 receptors)
海野 雄加; 佐藤 義則; 宮崎 千鶴; 永川 茂; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Mar. 2019
日本細菌学会 - Acinetobacter baumannii臨床分離株の肺感染モデルを用いた病原性解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Mar. 2019
日本細菌学会 - Acinetobacter baumannii感染klothoマウスにおける免疫応答の解析(Analysis of immune responses in klotho mice infected with Acinetobacter baumannii)
佐藤 義則; 永川 茂; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Mar. 2019
日本細菌学会 - 入院時監視培養により一人の患者から分離された3種のカルバペネマーゼ産生菌及び1種のESBL産生菌の分子疫学解析
西田智; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 浅原美和; 石垣しのぶ; 古川泰司; 斧康雄
感染症学雑誌, 01 Mar. 2019 - 微生物感染とヒト好中球内の炎症増強因子TREM1遺伝子発現との関係
Jan. 2019 - 本邦初のpan‐resistant Klebsiella pneumoniaeの全ゲノム解析
西田智; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 石垣しのぶ; 浅原美和; 古川泰司; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Jan. 2019 - Klothoマウスを用いたAcinetobacter baumannii感染マウスモデルの構築
Jan. 2019 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
Jan. 2019 - レゾルビンによる好中球活性化のプライミング反応
Jan. 2019, [Reviewed] - The pathogenicity analysis of clinical isolate Acinetobacter baumannii with pulmonary infection model
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
日本細菌学雑誌(Web), 2019 - Acinetobacter baumanniiの好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成阻害
鴨志田剛; 上田たかね; 西田智; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, Sep. 2018 - 多剤耐性Acinetobacter baumannii バイオフィルム形成能‐ポリペプチド系抗菌薬のsub-MIC効果‐
Jun. 2018 - Acinetobacter baumannii由来リポ多糖による脂肪細胞の機能変化
May 2018 - ウイルス由来病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
May 2018 - コリスチン耐性Klebsiella pneumoniaeカルバペネマーゼ(KPC)産生肺炎桿菌が有する薬剤耐性遺伝子の解析
May 2018 - Acinetobacter baumanniiのバイオフィルム形成に及ぼす抗菌薬のsub-MIC効果
May 2018 - Acinetobacter baumanniiのマスト細胞上への結合標的分子の解析
上田たかね; 鴨志田剛; 祖母井庸之; 西田智; 永川茂; 中野竜一; 中野章代; 菊地弘敏; 斧康雄
日本化学療法学会雑誌, Apr. 2018 - コリスチン耐性KPC産生肺炎桿菌のロングリードシーケンサーを用いた分子疫学解析
西田智; 石垣しのぶ; 浅原美和; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 古川泰司; 斧康雄
日本化学療法学会雑誌, Apr. 2018 - Acinetobacter baumannii由来リポ多糖のアディポカイン発現に及ぼす影響
海野雄加; 佐藤義則; 永川茂; 中野章代; 中野章代; 中野竜一; 中野竜一; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, Apr. 2018 - Acinetobacter baumanniiの好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成阻害
Apr. 2018 - 高病原性多剤耐性アシネトバクターバウマニ(MDRA)の抗菌薬感受性と治療効果
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 病原体関連分子パターン(PAMPs)刺激によるヒト好中球内の炎症増強因子TREM1遺伝子発現変化
祖母井 庸之; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - ポリペプチド系抗菌薬のsub-MICsにおけるAcinetobacter baumannii臨床分離株のバイオフィルム形成量の変化
佐藤 義則; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 病原性Acinetobacter baumanniiは好中球細胞外トラップの形成を抑制する(Pathogenic Acinetobacter baumnnii inhibits the formation of neutrophil extracellular traps)
鴨志田 剛; 上田 たかね; 西田 智; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - Acinetobacter baumanniiはCD32への接着を介して肥満細胞の炎症応答を誘導する(Acinetobacter baumannii induced the inflammatory response of mast cells via adhesion to CD32)
上田 たかね; 祖母井 庸之; 鴨志田 剛; 中野 竜一; 中野 章代; 永川 茂; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 高病原性多剤耐性アシネトバクターバウマニ(MDRA)の抗菌薬感受性と治療効果
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 病原体関連分子パターン(PAMPs)刺激によるヒト好中球内の炎症増強因子TREM1遺伝子発現変化
祖母井 庸之; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - ポリペプチド系抗菌薬のsub-MICsにおけるAcinetobacter baumannii臨床分離株のバイオフィルム形成量の変化
佐藤 義則; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2018
日本細菌学会 - 無血清培養による自発的な好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成
鴨志田剛; 上田たかね; 西田智; 永川茂; 菊地弘敏; 祖母井庸之; 斧康雄
日本生化学会大会(Web), Dec. 2017 - 無血清培養による好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成
鴨志田剛; 上田たかね; 西田智; 永川茂; 菊地弘敏; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017
(一社)日本感染症学会 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - 重症細菌感染症患者由来好中球の核の左方移動と炎症増強因子TREM1遺伝子発現の関係
祖母井庸之; 永川茂; 上田たかね; 鴨志田剛; 西田智; 佐藤義則; 海野雄加; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - Immunonutritionとしての各種不飽和脂肪酸の好中球機能に及ぼす影響
海野雄加; 永川茂; 佐藤義則; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - アシネトバクター・バウマニ感染マスト細胞培養上清中の好中球遊走活性作用の検討
上田たかね; 祖母井庸之; 鴨志田剛; 西田智; 永川茂; 佐藤義則; 海野雄加; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - 病原因子ompsの発現に相関したAcinetobacter baumannii臨床分離株の病原性変化
佐藤義則; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 永川茂; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - 重症細菌感染症患者由来好中球の核の左方移動と炎症増強因子TREM1遺伝子発現の関係
Mar. 2017 - 無血清培養による好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成
Mar. 2017 - 病原因子ompsの発現に相関したAcinetobacter baumannii臨床分離株の病原性変化
Mar. 2017 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
Mar. 2017 - Immunonutritionとしての各種不飽和脂肪酸の好中球機能に及ぼす影響
Mar. 2017 - アシネトバクター・バウマニ感染マスト細胞培養上清中の好中球遊走活性作用の検討
Mar. 2017 - 海外から持ち込まれる耐性菌 One Healthを踏まえた対策 入院時監視培養により分離されたコリスチン耐性Klebsiella pneumoniaeカルバペネマーゼ(KPC)産生肺炎桿菌の解析
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 浅原 美和; 石垣 しのぶ; 古川 泰司; 斧 康雄
日本化学療法学会雑誌, Mar. 2017
(公社)日本化学療法学会 - 細菌は無血清培養条件で誘発される自然好中球細胞外トラップに取り込まれる(Bacteria are trapped in spontaneous neutrophil extracellular traps induced by serum-free culture condition)
鴨志田 剛; 上田 たかね; 西田 智; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - 多剤耐性アシネトバクターバウマニ(MDRA)の病原性評価
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - Acinetobacter baumannii感染における好中球の炎症反応に関わる遺伝子の応答
上田 たかね; 祖母井 庸之; 鴨志田 剛; 永川 茂; 西田 智; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - 細菌感染症の患者病態に関連する好中球の核左方移動と炎症増強因子TREM1遺伝子発現
祖母井 庸之; 永川 茂; 上田 たかね; 鴨志田 剛; 西田 智; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - LPSは脂肪細胞とマクロファージの共培養系でMIP2産生を促進する
海野 雄加; 佐藤 義則; 永川 茂; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2017
日本細菌学会 - 多剤耐性Acinetobacter baumannii(MDRA)の病原性及び抗菌薬による治療効果の昆虫代替モデル用いた評価
Jan. 2017 - 病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
Jan. 2017 - Helicobacter pylori由来LPSがヒト好中球内の免疫関連遺伝子発現に及ぼす影響
Jan. 2017 - アシネトバクター刺激に対する好中球のNOX2と炎症反応に関わる遺伝子の応答
Jan. 2017 - 多剤耐性Acinetobacter baumannii(MDRA)の病原性及び抗菌薬による治療効果の昆虫代替モデル用いた評価
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
感染症学雑誌, Jan. 2017
(一社)日本感染症学会 - 病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井 庸之; 小澁 陽司; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
感染症学雑誌, Jan. 2017
(一社)日本感染症学会 - Helicobacter pylori由来LPSがヒト好中球内の免疫関連遺伝子発現に及ぼす影響
小澁 陽司; 祖母井 庸之; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
感染症学雑誌, Jan. 2017
(一社)日本感染症学会 - アシネトバクター刺激に対する好中球のNOX2と炎症反応に関わる遺伝子の応答
上田 たかね; 祖母井 庸之; 鴨志田 剛; 永川 茂; 西田 智; 海野 雄加; 佐藤 義則; 斧 康雄
感染症学雑誌, Jan. 2017
(一社)日本感染症学会 - 好中球を利用した新規細菌移動メカニズムBacterial immunity taxiの解析
鴨志田 剛; 上田 たかね; 永川 茂; 西田 智; 祖母井 庸之; 斧 康雄
日本生化学会大会プログラム・講演要旨集, Sep. 2016
(公社)日本生化学会 - 臨床分離されたAcinetobacter baumanniiのバイオフィルム形成に及ぼす抗菌薬のsub‐MIC効果
佐藤義則; 西田智; 永川茂; 上田たかね; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 09 May 2016 - LPS活性化ヒト好中球内の遺伝子発現に及ぼすケトライド系抗菌薬の影響
May 2016 - 多剤耐性Acinetobacter baumanniiは成熟脂肪細胞に作用して炎症反応を惹起する
May 2016 - 臨床分離されたAcinetobacter baumanniiの宿主細胞に及ぼす病原性の解析
May 2016 - アディポカイン発現に対する多剤耐性Acintobacter baumannii由来リポ多糖の影響
海野雄加; 佐藤義則; 西田智; 中野竜一; 中野竜一; 永川茂; 上田たかね; 鴨志田剛; 祖母井庸之; 斧康雄
感染症学雑誌, 20 Mar. 2016 - Interleukin‐8を介し活性化した好中球を利用したAcinetobacter baumanniiの新規細菌移動メカニズム
鴨志田剛; 上田たかね; 永川茂; 西田智; 佐藤義則; 海野雄加; 祖母井庸之; 斧康雄
感染症学雑誌, 20 Mar. 2016 - 多剤耐性アシネトバクター由来LPSがヒト好中球内で炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井庸之; 永川茂; 上田たかね; 鴨志田剛; 西田智; 佐藤義則; 海野雄加; 斧康雄
感染症学雑誌, Mar. 2016 - Acinetobacter baumannii刺激に対するマスト細胞と好中球の炎症反応に関わる遺伝子の応答
上田たかね; 祖母井庸之; 鴨志田剛; 永川茂; 西田智; 斧康雄; 海野雄加; 佐藤義則
感染症学雑誌, Mar. 2016 - Acinetobacter baumanniiの外膜タンパクOmpAの肺上皮細胞に及ぼす影響
佐藤義則; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, Mar. 2016 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
感染症学雑誌, Mar. 2016 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2016
日本細菌学会 - 脂肪細胞のアディポカイン発現に対するグラム陰性桿菌由来リポ多糖の影響
海野 雄加; 佐藤 義則; 西田 智; 永川 茂; 鴨志田 剛; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2016
日本細菌学会 - ヒト由来マスト細胞株のAcinetobacter生菌及びLPSに対する応答
上田 たかね; 祖母井 庸之; 中野 竜一; 鴨志田 剛; 永川 茂; 彦坂 健児; 斧 康雄; 中野 章代
感染症学雑誌, Mar. 2015
(一社)日本感染症学会 - アシネトバクターバウマニ臨床分離株の肺感染マウスを用いた病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 中野 竜一; 彦坂 健児; 斧 康雄
感染症学雑誌, Mar. 2015
(一社)日本感染症学会 - 好中球を利用したアシネトバクターの新たな細菌移動メカニズムの解析
鴨志田 剛; 上田 たかね; 永川 茂; 彦坂 健児; 中野 竜一; 祖母井 庸之; 斧 康雄
感染症学雑誌, Mar. 2015
(一社)日本感染症学会 - 細菌感染症患者好中球内で変動するTREM1遺伝子発現の定量解析とTREM1短鎖ペプチドが好中球機能に及ぼす影響
祖母井庸之; 永川茂; 上田たかね; 中野竜一; 越尾修; 斧康雄
日本細菌学雑誌, 25 Feb. 2013 - Effect of Oral Administration of β-D-glucan from Aureobasidium pullulans ADK-34 on Candida and MRSA Infections in Immunosuppressed Mice
TANIOKA Asuka; HAYAMA Kazumi; MITSUYA Masayasu; TANSHO Shigeru; ONO Yasuo; TSUBAKI Kazufumi; ABE Shigeru
Medical Mycology Journal, 31 Jan. 2012
We examined the effect of the oral administration of β-D-glucan derived from Aureobasidium pullulans ADK-34 (AP-FBG) on Candida albicans or methicillin-resistant Staphylococcus aureus (MRSA) infection in immunosuppressed mice. Mice pretreated with cyclophosphamide (CY) were intraperitoneally administered AP-FBG for 4 days and then infected with 6×104 C. albicans cells. In a preliminary experiment, the survival time of the Candida-infected mice treated with AP-FBG was clearly prolonged. Similarly, the effect of the oral administration of AP-FBG was examined. Mice were orally given 2.5% AP-FBG in feed for 42 days from 14 days prior to 2×104 C. albicans cells infection. The survival time of mice treated with AP-FBG was significantly prolonged and the viable cell count in the kidneys of the survivors was significantly decreased at 30 days after infection. The effects of the oral administration of AP-FBG on intestinal MRSA infection were also examined. Mice were given 2.5% AP-FBG orally in feed for 30 days before and after oral MRSA infection and treated with CY 12 days after the infection. The number of viable MRSA cells or the IgA production in feces did not significantly change, while AP-FBG administration seemed to relieve temporally the loss of body weight of mice. Conclusions: These results suggest that oral pre-administration of AP-FBG promoted resistance of CY-treated mice to C. albicans and lessened the weight reduction of CY-mice infected by MRSA., The Japanese Society for Medical Mycology - 細菌感染症患者の好中球において発現変化している遺伝子の解析
25 Feb. 2007 - 細菌感染症患者由来の好中球における MAPK family の燐酸化活性の検討
25 Feb. 2007 - 細菌感染症に伴う好中球の細胞膜受容体の変化
25 Feb. 2007 - アフラトキシンB1処理によりヒト肝臓癌細胞株で発現変化する癌関連遺伝子の解析
Jan. 2007 - Transferability of VanA Gene from Vancomycin-Resistant Enterococcus faecalis in the Digestive Tract of Specific Pathogen-Free Mice
IKEDA Tatsuo; WATANABE Takeshi; MATSUMOTO Kaoru; MURAYAMA Somay Y; KOSHIO Osamu; TANSHO Shigeru; ONO Yasuo
Journal of the Japanese Association for Infectious Diseases, 20 Nov. 2004
We evaluated the transferability of vanA gene from vancomycin-resistant Enterococcus faecalis (VREF) to vancomycin-sensitive E. faecalis (VSEF) in vitro and in vivo. In vitro conjugal transfer experiment by filter mating, the vanA gene of VREF was transferable at the high frequency to VSEF and a mutant strain which cured vanA gene of VREF. In vivo studies in the digestive tract of specific pathogen-free mice pretreated with oral antibiotics, transconjugants were also detected from the feces of a mouse at the lower frequency. However, the colonization of transconjugants was transient. The vanA gene in the donor and the transconjugant strain was confirmed by using a polymerase chain reaction method.
These results suggest that VSEF colonizing in the human digestive tract might be developed to VREF by transferring of the vanA gene., The Japanese Association for Infectious Diseases - Prophylactic Efficacy of A Basidiomycetes Preparation AHCC against Lethal Candida albicans Infection in Experimental Granulocytopenic Mice
IKEDA Tatsuo; ISHIBASHI Hiroko; FUJISAKI Ryuichi; YAMAZAKI Masatoshi; WAKAME Kohji; KOSUNA Kenichi; YAMAGUCHI Hideyo; ONO Yasuo; ABE Shigeru
Apr. 2003 - Murine IgE Antibody to Recognize Human Major Allergen, Mal f4
MITSUYA Masayasu; SUEGARA Nobuo; WADA Kayoko; IKEDA Tatsuo; MURAYAMA Somey Y; TANSHO Shigeru; YAMAGUCHI Hideyo; ONO Yasuo
Japanese Journal of Medical Mycology, 30 Oct. 2001
To elucidate the specificity of murine IgE antibody against Malassezia furfur, the immunoblot patterns of IgE in sera obtained from mice inoculated repeatedly in the nasal cavity with M. f. cells, or injected intraperitoneally with M. f. cells mixed with Al(OH)3 gel were compared with those of IgE antibody detected in sera from patients with AD. Most of the murine IgE anti-Malassezia antibodies shared some antigenic bands with IgE in sera from patients with AD. The IgE antibody of murine also recognized Mal f4, one of the major allergens detected in patients with AD., The Japanese Society for Medical Mycology - In Vitro Assay Method for Augmention of Anti-Candida Activity of Murine Bone Marrow Cells by Cytokines
MOROFUJI Shinichiro; ABE Shigeru; TANSHO Shigeru; ONO Yasuo; YAMAGUCHI Hideyo; OKINAGA Kota
Apr. 2001 - Suppressive Effect of Lansoprazole on Anti-Candida Activity of Murine Macrophages
MOTEGI Hideto; ABE Shigeru; TANSHO Shigeru; SUZUKI Daisuke; YAMAGUCHI Hideyo; HOSHINO Etsuo
20 Feb. 2001 - Prophylactic efficacy of a basidiomycetes preparation AHCC against lethal opportunistic infections in mice
H Ishibashi; T Ikeda; S Tansho; Y Ono; M Yamazaki; A Sato; K Yamaoka; H Yamaguchi; S Abe
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, Aug. 2000 - PP-514 骨髄細胞の抗真菌活性に及ぼす各種サイトカインの影響
10 Mar. 2000 - Suppressive effects of lanoconazole on arthus phenomenon in vivo and on production and functions of TNF in vitro
M. Mitsuya; K. Wada; H. Ishibashi; S. Tansho; S. Abe; H. Yamaguchi
Japanese Journal of Medical Mycology, 2000
Japanese Society for Medical Mycology - Protective effect of oral administration of several traditional kampo- medicines on lethal Candida infection in immunosuppressed mice
S. Abe; H. Ishibashi; S. Tansho; R. Hanazawa; Y. Komatsu; H. Yamaguchi
Japanese Journal of Medical Mycology, 2000
Japanese Society for Medical Mycology - Anti-candida activities of azole antifungals in the presence of lysozyme in vitro
S. Abe; S. Tansho; P. D. Perera; T. Hiratani; Y. Nishiyama; K. Uchida; H. Yamaguchi
Japanese Journal of Medical Mycology, 1998
Japanese Society for Medical Mycology - Repression of the capacity to produce tumor necrosis factor alpha in candida albicans-infected mice and reversal of the repression by oral Juzen- taiho-to treatment
S. Abe; G. Akagawa; S. Tansho; H. Ochi; M. Osumi; Y. Komatsu; K. Uchida; H. Yamaguchi
Japanese Journal of Medical Mycology, 1997
Japanese Society for Medical Mycology - Roles of Activated Macrophages in Host Defense Mechanisms Against Candida Infection
Shigeru Abe; Shigeru Tansho; Hideyo Yamaguchi; Katsuhisa Uchida; Hideyo Yamaguchi
Japanese Journal of Medical Mycology, 1997 - Suppression of Anti-Candida Activity of Human Neutrophils by Glucose and Diminishment of the Glucose Effect by an Amino Acid Mixture
TANSHO Tohru; OKINAGA Kota; TANSHO Shigeru; ABE Shigeru; YAMAGUCHI Hideyo
Journal of the Japanese Association for Infectious Diseases, 20 May 1996
Effects of. glucose and amino acid mixture prescribed for parenteral alimentation on atni-Candida activity of neutrophils were examined. Neutrophils obtained from peripheral blood of healthy humans inhibited the growth of Candida albicans in vitro. More than 1.0% of glucose inhibited the anti-Candida activity of the neutrophils in. dose-dependent manner. This glucose effect was reduced by the addition of an amino acid mixture clinically prescribed with. carbohydrate solution (PN-twin) in Japan. The amino acid mixture neutralized the suppression of anti-Candida activity of neutrophils by dexamethasone. These results suggest that an amino acid mixture prescribed in an alimentation solution may play. role as. neutralizer of the suppressive action of glucose for anti-Candida activity of neutrophils in. limitted area near the top of. catheter in. blood vessel., The Japanese Association for Infectious Diseases - Augmentation of anti-Candida activity of lanoconazole by lysozyme
S. Tansho; T. Tansho; T. Ikeda; P. D. Perera; S. Abe; H. Yamaguchi
Japanese Journal of Medical Mycology, 1996
Japanese Society for Medical Mycology
Books and other publications
Lectures, oral presentations, etc.
- 学生実習の効率化と実習理解度の変化 コロナ禍を経験して
Jul. 2024 - ヒト早発性老化症候群モデルマウスを用いたAcinetobacter baumannii感染に対する肺の免疫応答の解析
Jan. 2024 - 臨床分離されたStaphylococcus haemolyticusのバイオフィルム形成に及ぼすテイコプラニンの効果
Mar. 2023 - 臨床分離されたカルバペネム耐性菌におけるコリスチン耐性の解析
Mar. 2023 - 多剤耐性Acinetobacter baumanniiにおけるブレイクポイント・チェッカーボードプレートを用いた薬剤併用効果の検討
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌臨床分離株のバイオフィルム形成能の評価
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌が形成するバイオフィルムに対するメロぺネム(MEPM)の有効性評価
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌が形成するバイオフィルムに対するメロぺネム(MEPM)の有効性評価
Mar. 2023 - 臨床分離されたStaphylococcus haemolyticusのバイオフィルム形成に及ぼすテイコプラニンの効果
Mar. 2023 - 臨床分離されたカルバペネム耐性菌におけるコリスチン耐性の解析
Mar. 2023 - 多剤耐性Acinetobacter baumanniiにおけるブレイクポイント・チェッカーボードプレートを用いた薬剤併用効果の検討
Mar. 2023 - 基質特異性拡張型βラクタマーゼ(ESBL)産生大腸菌臨床分離株のバイオフィルム形成能の評価
Mar. 2023 - 分類・疫学・感染症 CRISPR-Cas12aを用いた多剤耐性アシネトバクターのカルバペネマーゼ遺伝子検査
Feb. 2023 - 健常な学生集団から回収されたメチシリン耐性ブドウ球菌の解析
Feb. 2023 - 分類・疫学・感染症 CRISPR-Cas12aを用いた多剤耐性アシネトバクターのカルバペネマーゼ遺伝子検査
Feb. 2023 - 健常な学生集団から回収されたメチシリン耐性ブドウ球菌の解析
Feb. 2023 - CRISPR-Cas12a system for carbapenemase gene detection of multidrug-resistant Acinetobacter
古賀美沙希; 西田智; 永川茂; 上田たかね; 佐藤義則; 斧康雄; 斧康雄; 吉野友祐
日本細菌学雑誌(Web), 2023 - メタロ-β-ラクタマーゼIMP-1産生Klebsiella michiganensisのプラスミド解析
May 2022 - チゲサイクリン存在下におけるヒト好中球の多剤耐性Acinetobacter baumanniiに対する殺菌効果の解析
Mar. 2022 - チゲサイクリン存在下におけるヒト好中球の多剤耐性Acinetobacter baumanniiに対する殺菌効果の解析
Mar. 2022 - 多剤耐性Acinetobacter baumanniiにおける新規抗菌薬セフィデロコル有用性の検討
2022 - チゲサイクリンは多剤耐性Acinetobacter baumanniiの病原因子を抑制し,ヒト好中球による殺菌を可能にする
2022 - 多剤耐性Acinetobacter baumanniiのバイオフィルムに対するコリスチン/チゲサイクリンの併用効果
Sep. 2021 - メタロ-β-ラクタマーゼIMP-1産生Klebsiella michiganensisのゲノム解析
Apr. 2021 - 高齢者におけるAcinetobacter baumannii感染重症化機序の解明(ヒト早発性老化症候群モデルマウスによる感染免疫応答の解析)
Apr. 2021 - メタロ-β-ラクタマーゼIMP-1産生Klebsiella michiganensisのゲノム解析
Apr. 2021 - Galectin-8のヒト好中球機能に及ぼす影響
Mar. 2020 - マクロファージの殺菌に対するAcinetobacter baumanniiの抵抗性評価
Mar. 2020 - 経皮経肝胆道ドレナージ患者におけるOXA-48型カルバペネマーゼ産生E.coliの出現(Emergence of E. coli producing OXA-48-like carbapenemase in a patient with percutaneous transhepatic biliary drainage)
西田 智; 上田 たかね; 祖母井 庸之; 永川 茂; 佐藤 義則; 石垣 しのぶ; 古川 泰司; 斧 康雄; 浅原 美和
感染症学雑誌, Mar. 2020, (一社)日本感染症学会 - ヒト早発性老化症候群モデルマウスを用いたAcinetobacter baumannii感染に対する免疫応答の解析
Mar. 2020 - ヒト好中球機能に及ぼすGalectin-8の影響
Mar. 2020 - 電撃紫斑病患者から分離された肺炎球菌の全ゲノム解析
Mar. 2020 - Emergence of E. coli producing OXA-48-like carbapenemase in a patient with percutaneous transhepatic biliary drainage(和訳中)
西田 智; 上田 たかね; 祖母井 庸之; 永川 茂; 佐藤 義則; 石垣 しのぶ; 古川 泰司; 斧 康雄; 浅原 美和
感染症学雑誌, Mar. 2020, (一社)日本感染症学会 - ヒト早発性老化症候群モデルマウスを用いたAcinetobacter baumannii感染に対する免疫応答の解析
Mar. 2020 - ヒト好中球機能に及ぼすGalectin-8の影響
Mar. 2020 - 電撃紫斑病患者から分離された肺炎球菌の全ゲノム解析
Mar. 2020 - Galectin-8のヒト好中球機能に及ぼす影響
Mar. 2020 - マクロファージの殺菌に対するAcinetobacter baumanniiの抵抗性評価
Mar. 2020 - Draft genome sequences of two strains of Streptococcus pneumoniae from AIPF patients
上田たかね; 藤崎竜一; 祖母井庸之; 西田智; 永川茂; 佐藤義則; 斧康雄
日本細菌学雑誌(Web), 2020 - 分類・疫学・感染症 汎薬剤耐性菌Klebsiella pneumoniaeシークンエンス11型のゲノムの分析(Taxonomy, Epidemiology, and Infectious Diseases Genomic analysis of a pandrug-resistant Klebsiella pneumoniae sequence type 11)
西田 智; 上田 たかね; 祖母井 庸之; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Jan. 2020, 日本細菌学会 - Multidrug-resistant A.baumanniiは活性酸素種に抵抗してマクロファージ内で生存する(Multidrug-resistant A. baumannii resists reactive oxygen species and survives in macrophages)
佐藤 義則; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Jan. 2020, 日本細菌学会 - AIPF患者から分離されたStreptococcus pneumoniaeの2株の暫定ゲノム配列(Draft genome sequences of two strains of Streptococcus pneumoniae from AIPF patients)
Jan. 2020 - 分類・疫学・感染症(Taxonomy, Epidemiology, and Infectious Diseases Genomic analysis of a pandrug-resistant Klebsiella pneumoniae sequence type 11)
西田 智; 上田 たかね; 祖母井 庸之; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Jan. 2020, 日本細菌学会 - Draft genome sequences of two strains of Streptococcus pneumoniae from AIPF patients(和訳中)
Jan. 2020 - コリスチン耐性によりリポ多糖を欠損したAcinetobacter baumanniiは好中球が産生するリゾチームにより殺菌される
Sep. 2019 - レゾルビンE1によるヒト好中球の活性酸素産生活性化機構の解明(Resolvin E1 enhances fMLF-induced ROS generation via LTB4 receptors)
海野 雄加; 佐藤 義則; 宮崎 千鶴; 永川 茂; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Mar. 2019, 日本細菌学会 - Acinetobacter baumannii臨床分離株の肺感染モデルを用いた病原性解析
Mar. 2019 - Acinetobacter baumannii感染klothoマウスにおける免疫応答の解析(Analysis of immune responses in klotho mice infected with Acinetobacter baumannii)
佐藤 義則; 永川 茂; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Mar. 2019, 日本細菌学会 - 高齢宿主を想定したAcinetobacter baumannii感染に対する免疫応答の解析
佐藤義則; 永川茂; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
感染症学雑誌, 01 Mar. 2019 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性解析
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
感染症学雑誌, 01 Mar. 2019 - コリスチン耐性Acinetobacter baumanniiと好中球の相互作用解析
鈴木雄介; 鴨志田剛; 赤路卓哉; 上田たかね; 西田智; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, 01 Mar. 2019 - コリスチン耐性Acinetobacter baumannii菌株の樹立と性状解析
赤路卓哉; 鴨志田剛; 鈴木雄介; 西田智; 上田たかね; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, 01 Mar. 2019 - 微生物感染とヒト好中球内の炎症増強因子TREM1遺伝子発現との関係
Jan. 2019 - Klothoマウスを用いたAcinetobacter baumannii感染マウスモデルの構築
Jan. 2019 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
Jan. 2019 - レゾルビンによる好中球活性化のプライミング反応
Jan. 2019 - 微生物感染とヒト好中球内の炎症増強因子TREM1遺伝子発現との関係
祖母井庸之; 永川茂; 上田たかね; 西田智; 鴨志田剛; 佐藤義則; 海野雄加; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2018 - Klothoマウスを用いたAcinetobacter baumannii感染マウスモデルの構築
佐藤義則; 永川茂; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2018 - レゾルビンによる好中球活性化のプライミング反応
海野雄加; 佐藤義則; 永川茂; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2018 - 本邦初のpan‐resistant Klebsiella pneumoniaeの全ゲノム解析
西田智; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 石垣しのぶ; 浅原美和; 古川泰司; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2018 - 病原性細菌Acinetobacter baumnniiの好中球接着抑制によるneutrophil extracellular traps(NETs)形成阻害
Sep. 2018 - Acinetobacter baumannii由来リポ多糖による脂肪細胞の機能変化
May 2018 - ウイルス由来病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
May 2018 - コリスチン耐性Klebsiella pneumoniaeカルバペネマーゼ(KPC)産生肺炎桿菌が有する薬剤耐性遺伝子の解析
May 2018 - Acinetobacter baumanniiのバイオフィルム形成に及ぼす抗菌薬のsub-MIC効果
May 2018 - Acinetobacter baumanniiのマスト細胞上への結合標的分子の解析
上田たかね; 鴨志田剛; 祖母井庸之; 西田智; 永川茂; 中野竜一; 中野章代; 菊地弘敏; 斧康雄
日本化学療法学会雑誌, 25 Apr. 2018 - 多剤耐性Acinetobacter baumanniiのバイオフィルム形成能―ポリペプチド系抗菌薬のsub‐MIC効果―
佐藤義則; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 永川茂; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 25 Apr. 2018 - コリスチン耐性KPC産生肺炎桿菌のロングリードシーケンサーを用いた分子疫学解析
西田智; 石垣しのぶ; 浅原美和; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 古川泰司; 斧康雄
日本化学療法学会雑誌, 25 Apr. 2018 - Acinetobacter baumanniiの好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成阻害
鴨志田剛; 上田たかね; 西田智; 佐藤義則; 海野雄加; 永川茂; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 25 Apr. 2018 - Acinetobacter baumannii由来リポ多糖のアディポカイン発現に及ぼす影響
海野雄加; 佐藤義則; 永川茂; 中野章代; 中野章代; 中野竜一; 中野竜一; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本化学療法学会雑誌, 25 Apr. 2018 - 病原性Acinetobacter baumanniiは好中球細胞外トラップの形成を抑制する(Pathogenic Acinetobacter baumnnii inhibits the formation of neutrophil extracellular traps)
鴨志田 剛; 上田 たかね; 西田 智; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - 高病原性多剤耐性アシネトバクターバウマニ(MDRA)の抗菌薬感受性と治療効果
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - Acinetobacter baumanniiはCD32への接着を介して肥満細胞の炎症応答を誘導する(Acinetobacter baumannii induced the inflammatory response of mast cells via adhesion to CD32)
上田 たかね; 祖母井 庸之; 鴨志田 剛; 中野 竜一; 中野 章代; 永川 茂; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - 病原体関連分子パターン(PAMPs)刺激によるヒト好中球内の炎症増強因子TREM1遺伝子発現変化
祖母井 庸之; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - ポリペプチド系抗菌薬のsub-MICsにおけるAcinetobacter baumannii臨床分離株のバイオフィルム形成量の変化
佐藤 義則; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - 高病原性多剤耐性アシネトバクターバウマニ(MDRA)の抗菌薬感受性と治療効果
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - 病原体関連分子パターン(PAMPs)刺激によるヒト好中球内の炎症増強因子TREM1遺伝子発現変化
祖母井 庸之; 永川 茂; 上田 たかね; 西田 智; 鴨志田 剛; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - ポリペプチド系抗菌薬のsub-MICsにおけるAcinetobacter baumannii臨床分離株のバイオフィルム形成量の変化
佐藤 義則; 海野 雄加; 鴨志田 剛; 西田 智; 上田 たかね; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2018, 日本細菌学会 - ポリペプチド系抗菌薬のsub-MICsにおけるAcinetobacter baumannii臨床分離株のバイオフィルム形成量の変化
2018 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
2018 - コリスチン耐性Klebsiella pneumoniaeカルバペネマーゼ(KPC)産生肺炎桿菌が有する薬剤耐性遺伝子の解析
西田智; 石垣しのぶ; 浅原美和; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 古川泰司; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2017 - ウイルス由来病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井庸之; 永川茂; 上田たかね; 西田智; 鴨志田剛; 佐藤義則; 海野雄加; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2017 - Acinetobacter baumannii由来リポ多糖による脂肪細胞の機能変化
海野雄加; 佐藤義則; 永川茂; 鴨志田剛; 西田智; 上田たかね; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2017 - Acinetobacter baumanniiのバイオフィルム形成に及ぼす抗菌薬のsub‐MIC効果
佐藤義則; 西田智; 永川茂; 上田たかね; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2017 - Acinetobacter baumanniiの好中球を利用した新規細菌メカニズムBacterial immunity taxiについて
鴨志田剛; 永川茂; 上田たかね; 中野竜一; 中野竜一; 彦坂健児; 彦坂健児; 西田智; 祖母井庸之; 東昌市; 斧康雄
微生物シンポジウム講演要旨集, 29 Aug. 2017 - 無血清培養による好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成
Apr. 2017 - 入院時監視培養により分離されたコリスチン耐性Klebsiella pneumoniaeカルバペネマーゼ(KPC)産生肺炎桿菌の解析
西田智; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 浅原美和; 石垣しのぶ; 古川泰司; 斧康雄
日本化学療法学会雑誌, 03 Mar. 2017 - 重症細菌感染症患者由来好中球の核の左方移動と炎症増強因子TREM1遺伝子発現の関係
Mar. 2017 - 病原因子ompsの発現に相関したAcinetobacter baumannii臨床分離株の病原性変化
Mar. 2017 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
Mar. 2017 - Immunonutritionとしての各種不飽和脂肪酸の好中球機能に及ぼす影響
Mar. 2017 - アシネトバクター・バウマニ感染マスト細胞培養上清中の好中球遊走活性作用の検討
Mar. 2017 - LAMP法によるカルバペネマーゼ産生アシネトバクター迅速検出法の開発
中野 竜一; Mu Xiaoqin; 中野 章代; 鴨志田 剛; 祖母井 庸之; 永川 茂; 上田 たかね; 遠藤 史郎; 矢野 寿一; 斧 康雄
日本化学療法学会雑誌, Mar. 2017, (公社)日本化学療法学会 - 細菌は無血清培養条件で誘発される自然好中球細胞外トラップに取り込まれる(Bacteria are trapped in spontaneous neutrophil extracellular traps induced by serum-free culture condition)
鴨志田 剛; 上田 たかね; 西田 智; 永川 茂; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - Acinetobacter baumannii感染における好中球の炎症反応に関わる遺伝子の応答
上田 たかね; 祖母井 庸之; 鴨志田 剛; 永川 茂; 西田 智; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - 多剤耐性アシネトバクターバウマニ(MDRA)の病原性評価
西田 智; 上田 たかね; 祖母井 庸之; 海野 雄加; 鴨志田 剛; 佐藤 義則; 永川 茂; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - 細菌感染症の患者病態に関連する好中球の核左方移動と炎症増強因子TREM1遺伝子発現
祖母井 庸之; 永川 茂; 上田 たかね; 鴨志田 剛; 西田 智; 佐藤 義則; 海野 雄加; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - LPSは脂肪細胞とマクロファージの共培養系でMIP2産生を促進する
海野 雄加; 佐藤 義則; 永川 茂; 鴨志田 剛; 西田 智; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2017, 日本細菌学会 - LPSは脂肪細胞とマクロファージの共培養系でMIP2産生を促進する
2017 - 肺感染マウスを用いたAcinetobacter baumannii臨床分離株の病原性の解析
2017 - 多剤耐性アシネトバクターバウマニ(MDRA)の病原性評価
2017 - Acinetobacter baumannii感染における好中球の炎症反応に関わる遺伝子の応答
2017 - 多剤耐性Acinetobacter baumannii(MDRA)の病原性及び抗菌薬による治療効果の昆虫代替モデル用いた評価
Jan. 2017 - 病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
Jan. 2017 - Helicobacter pylori由来LPSがヒト好中球内の免疫関連遺伝子発現に及ぼす影響
Jan. 2017 - アシネトバクター刺激に対する好中球のNOX2と炎症反応に関わる遺伝子の応答
Jan. 2017 - 無血清培養による自発的な好中球細胞外トラップ(neutrophil extracellular traps:NETs)形成
鴨志田剛; 上田たかね; 西田智; 永川茂; 菊地弘敏; 祖母井庸之; 斧康雄
日本生化学会大会(Web), 2017 - 好中球を利用した細菌の新規移動メカニズム:Bacterial immunity taxi
鴨志田剛; 上田たかね; 西田智; 永川茂; 祖母井庸之; 斧康雄
Pharmaco-Hematologyシンポジウム講演要旨集, 03 Sep. 2016 - 好中球を利用した新規細菌移動メカニズムBacterial immunity taxiの解析
Sep. 2016 - 病原体関連分子パターン(PAMPs)がヒト好中球内の炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井庸之; 小澁陽司; 永川茂; 上田たかね; 西田智; 鴨志田剛; 佐藤義則; 海野雄加; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2016 - アシネトバクター刺激に対する好中球のNOX2と炎症反応に関わる遺伝子の応答
上田たかね; 祖母井庸之; 鴨志田剛; 永川茂; 西田智; 海野雄加; 佐藤義則; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2016 - 多剤耐性Acinetobacter baumannii(MDRA)の病原性及び抗菌薬による治療効果の昆虫代替モデル用いた評価
西田智; 上田たかね; 祖母井庸之; 海野雄加; 鴨志田剛; 佐藤義則; 永川茂; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2016 - Helicobacter pylori由来LPSがヒト好中球内の免疫関連遺伝子発現に及ぼす影響
小澁陽司; 祖母井庸之; 永川茂; 上田たかね; 西田智; 鴨志田剛; 佐藤義則; 海野雄加; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2016 - LPS活性化ヒト好中球内で炎症増強因子TREM1遺伝子発現に及ぼす抗菌薬の影響
祖母井庸之; 小澁陽司; 永川茂; 上田たかね; 西田智; 佐藤義則; 斧康雄
日本化学療法学会雑誌, 09 May 2016 - 昆虫を用いた多剤耐性アシネトバクターバウマニ(MDRA)の病原性評価及び抗菌薬による治療効果
西田智; 上田たかね; 祖母井庸之; 佐藤義則; 永川茂; 斧康雄
日本化学療法学会雑誌, 09 May 2016 - LPS活性化ヒト好中球内の遺伝子発現に及ぼすケトライド系抗菌薬の影響
May 2016 - 多剤耐性Acinetobacter baumanniiは成熟脂肪細胞に作用して炎症反応を惹起する
May 2016 - Acinetobacter baumanniiの好中球を利用した新規細菌移動メカニズムの解析
May 2016 - 臨床分離されたAcinetobacter baumanniiの宿主細胞に及ぼす病原性の解析
May 2016 - Acinetobacter baumannii刺激に対するマスト細胞と好中球の炎症反応に関わる遺伝子の応答
上田たかね; 祖母井庸之; 鴨志田剛; 永川茂; 西田智; 斧康雄; 海野雄加; 佐藤義則
感染症学雑誌, 20 Mar. 2016 - 多剤耐性アシネトバクター由来LPSがヒト好中球内で炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井庸之; 永川茂; 上田たかね; 鴨志田剛; 西田智; 佐藤義則; 海野雄加; 斧康雄
感染症学雑誌, 20 Mar. 2016 - Acinetobacter baumanniiの外膜タンパクOmpAの肺上皮細胞に及ぼす影響
佐藤義則; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 永川茂; 祖母井庸之; 斧康雄
感染症学雑誌, 20 Mar. 2016 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川茂; 祖母井庸之; 上田たかね; 鴨志田剛; 佐藤義則; 海野雄加; 西田智; 斧康雄
感染症学雑誌, 20 Mar. 2016 - 本邦におけるCTX-M型βラクタマーゼ産生大腸菌の性状解析
中野 竜一; 彦坂 健児; 中野 章代; 鴨志田 剛; 上田 たかね; 永川 茂; 祖母井 庸之; 浅原 美和; 古川 泰司; 笠原 敬; 矢野 寿一; 斧 康雄
感染症学雑誌, Mar. 2016, (一社)日本感染症学会 - LAMP法によるカルバペネマーゼ産生アシネトバクター迅速検出法の開発
中野 竜一; 中野 章代; 祖母井 庸之; 永川 茂; 上田 たかね; 遠藤 史郎; 矢野 寿一; 斧 康雄; Mu Xiaoqin; 鴨志田 剛
日本化学療法学会雑誌, Mar. 2016, (公社)日本化学療法学会 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 鴨志田 剛; 佐藤 義則; 海野 雄加; 西田 智; 斧 康雄
日本細菌学雑誌, Feb. 2016, 日本細菌学会 - 脂肪細胞のアディポカイン発現に対するグラム陰性桿菌由来リポ多糖の影響
海野 雄加; 佐藤 義則; 西田 智; 永川 茂; 鴨志田 剛; 上田 たかね; 祖母井 庸之; 斧 康雄
日本細菌学雑誌, Feb. 2016, 日本細菌学会 - LAMP法によるカルバペネマーゼ産生アシネトバクター迅速検出法の開発
中野竜一; 中野章代; 祖母井庸之; 永川茂; 上田たかね; 遠藤史郎; 矢野寿一; 斧康雄
Oct. 2015 - 病原因子リポ多糖がヒト好中球内で炎症増強因子TREM1遺伝子発現に及ぼす影響
祖母井 庸之; 永川 茂; 上田 たかね; 中野 竜一; 鴨志田 剛; 彦坂 健児; 斧 康雄
感染症学雑誌, Mar. 2015, (一社)日本感染症学会 - Acinetobacter baumannii臨床分離株の肺感染マウスを用いた病原性の解析
永川 茂; 祖母井 庸之; 上田 たかね; 中野 竜一; 鴨志田 剛; 彦坂 健児; 斧 康雄
感染症学雑誌, Mar. 2015, (一社)日本感染症学会 - アシネトバクターの新規病原性 好中球を利用した新たな細菌移動メカニズム
鴨志田 剛; 上田 たかね; 永川 茂; 彦坂 健児; 中野 竜一; 祖母井 庸之; 斧 康雄
感染症学雑誌, Mar. 2015, (一社)日本感染症学会 - CTX-M型βラクタマーゼ産生大腸菌におけるプラスミドの性状解析
彦坂 健児; 中野 竜一; 鴨志田 剛; 上田 たかね; 永川 茂; 祖母井 庸之; 浅原 美和; 古川 泰司; 斧 康雄; 中野 章代
感染症学雑誌, Mar. 2015, (一社)日本感染症学会 - カルバペネマーゼ産生アシネトバクターを検出する新規LAMP法の開発
中野 竜一; 浅原 美和; 古川 泰司; 彦坂 健児; 鴨志田 剛; 祖母井 庸之; 永川 茂; 上田 たかね; 斧 康雄; Mu Xiaoqin; 中野 章代
感染症学雑誌, Mar. 2015, (一社)日本感染症学会 - 臨床分離されたAcinetobacter baumanniiの宿主細胞に及ぼす病原性の解析
佐藤義則; 海野雄加; 鴨志田剛; 西田智; 上田たかね; 永川茂; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, 2015 - 多剤耐性Acinetobacter baumanniiは成熟脂肪細胞に作用して炎症反応を惹起する
海野雄加; 佐藤義則; 西田智; 中野竜一; 中野章代; 永川茂; 上田たかね; 鴨志田剛; 祖母井庸之; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, 2015 - LPS活性化ヒト好中球内の遺伝子発現に及ぼすケトライド系抗菌薬の影響
祖母井庸之; 小澁陽司; 永川茂; 上田たかね; 西田智; 佐藤義則; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, 2015 - 免疫能低下時に発症した粟粒結核3例のInterferon-γ Release Assaysと好中球CD64発現の検討
Mar. 2014 - 炎症性ミエロイド細胞受容体TREM‐1とヒト好中球の関係
祖母井庸之; 永川茂; 上田たかね; 中野竜一; 鴨志田剛; 菊地弘敏; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2013 - 免疫能低下時に発症した粟粒結核3例のInterferon‐γ Release Assaysと好中球CD64発現の検討
菊地弘敏; 永川茂; 鴨志田剛; 中野竜一; 上田たかね; 祖母井庸之; 西野仁樹; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2013 - 分化HL60でのTNF‐αprimingによるCD11b発現変化
上田たかね; 祖母井庸之; 永川茂; 菊地弘敏; 中野竜一; 鴨志田剛; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2013 - アシネトバクターと緑膿菌に対する好中球のNETs形成の差異
鴨志田剛; 上田たかね; 永川茂; 中野竜一; 祖母井庸之; 菊地弘敏; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Oct. 2013 - P-096 細菌感染症患者の末梢血好中球における抗原認識に関わる細胞膜抗原の解析(一般演題(ポスター発表),生態と進化から考える医真菌学)
10 Nov. 2012 - P-085 Candida albicans由来のβ-glucan 画分によるヒト好中球のERKおよびp38 MAPK燐酸化への影響(一般演題(ポスター発表),生態と進化から考える医真菌学)
10 Nov. 2012 - P-082 Aflatoxin (AF)B_1が自然免疫能と肝癌細胞株の細胞増殖能に及ぼす影響(一般演題(ポスター発表),生態と進化から考える医真菌学)
10 Nov. 2012 - 細菌感染症患者の好中球内TREM‐1遺伝子発現解析とsTREM‐1短鎖ペプチドが好中球に及ぼす影響
祖母井庸之; 永川茂; 上田たかね; 中野竜一; 菊地弘敏; 越尾修; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2012 - Mycobacterium xenopiによる大臀筋深部膿瘍の一例
菊地弘敏; 浅原美和; 田中孝志; 永川茂; 上田たかね; 祖母井庸之; 越尾修; 中野竜一; 川上小夜子; 斧康雄
日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集, Sep. 2012 - マウス好中球によるCandida発育阻止における一酸化窒素とニトロチロシンの役割
Feb. 2001 - Candida発育の好中球による阻止における一酸化窒素とニトロチロシンの役割
Oct. 2000 - 好中球によるCandida発育の阻止:一酸化窒素とニトロチロシンの役割
Apr. 2000
Affiliated academic society
Research Themes
- Analysis of the mechanisms of Acinetobacter infection in klotho knockout mice
Grant-in-Aid for Scientific Research (C)
Teikyo University
01 Apr. 2021 - 31 Mar. 2024 - 多剤耐性アシネトバクターの重症化に関与する病原因子解明と新規治療法/予防法の開発
01 Apr. 2020 - 31 Mar. 2023 - Study on the escape mechanism of multidrug-resistant bacteria from host defense mechanism and development of new therapeutic method
Grant-in-Aid for Scientific Research (C)
Teikyo University
01 Apr. 2017 - 31 Mar. 2020
Acinetobacter baumannii (A.b) suppresses NETs formation in neutrophils. MDRA clinical isolates have high catalase-producing ability and survive and proliferate in the phagosome even after being phagocytosed by macrophages. Co-culture of A.b and its lipopolysaccharide (LPS) with mast cells and adipocytes enhanced the production of inflammatory cytokines and chemokines. The sub-MICs of tigecycline suppressed the biofilm formation of MDRA, while colistin (CL) enhanced it. The virulence of the LPS-deficient A.b strain was reduced. We analyzed the resistance genes of multidrug-resistant strains such as KPC-producing Klebsiella pneumoniae isolated in our hospital. Pathological findings in A.b mouse pneumonia model were compared with Pseudomonas aeruginosa.
We established a drug efficacy evaluation system for antibacterial drugs using the MDRA insect infection model. - Analysis of the predisposing host factors to severe pathological conditions caused by Acinetobacter baumannii infections and the search the new virulence factors of multi-drug resistant strains
Grant-in-Aid for Scientific Research (C)
Teikyo University
01 Apr. 2012 - 31 Mar. 2015
1)Acinetobacter baumannii (A.baumannii) were resistant to phagocytosis and bactericidal activity by neutrophils as compared with Pseudomonas aeruginosa, neutrophil extracellular traps (NETs) formation was very little. 2) The lethality in a pulmonary infection mouse model caused by P. aeruginosa was higher than that of A.baumannii infection. 3) When neutrophils were stimulated with the lipopolysaccharides (LPS) derived from an A.baumannii standard strain or a multi-drug resistant strain (MDRA), gene expression levels of inflammatory cytokines in neutrophils were equally upregulated. Membrane vesicles derived from A.baumannii could not stimulate the production of reactive oxygen in neutrophils, but acted as a chemoattractant. 4) A. baumannii-derived LPS enhanced the TNF-α and IL-8 production in mast cells. 5) We have developed a LAMP method to detect MDRA. 6) Humoral immunodeficiency may be a predisposing factor to severe pathological conditions during A. baumannii infections. - Establishment of immuno-monitoring system for the infectious disease control and analysis of neutrophil dysfunction in various compromised hosts
Grant-in-Aid for Scientific Research (C)
Teikyo University
2009 - 2011
Neutrophil chemotaxis was reduced in septic patients and poorly controlled diabetic patients. Neutrophil bactericidal ability in the patients with liver cirrhosis, SLE, AIDS, myeloma, severe burn and elderly persons was significantly lower than that of healthy controls. Expression levels of neutrophil membrane antigens, CD14 and TLR-4 were higher in patients with sepsis ; however, the expression levels of CD11b and CD16 were lower. The gene expression of TREM-1 in neutrophils associated with the severity of sepsis. Analysis of neutrophil functions using these assays may be available as the sensitive and reliable immuno-monitoring tools for determining the capacity of host defense against bacterial infections in compromised hosts. - Effects of matification on immunity in hosts prohibited from oral alimentation
Grant-in-Aid for Scientific Research (C)
Teikyo University
1995 - 1996
Parenteral alimentation is clinically applied to patients with intestinal dysfunction who are prohibited from oral alimentation. Their immunological status is often greatly suppressed making them vulnerable to opportunistic fungal infection. In this study we focused on antifungal activity (growth inhibition activity against fungi) of neutrophils in hosts unable to take oral alimentation. Neutrophils obtained from peripheral blood of normal individuals clearly inhibited the growth of Candida albicans a in vitro.. In some patients with parenteral alimentation, the anti-Candida activity of neutrophils appeared to be suppressed, but variations in the activities among patients with different diseases made clear observation impossible. The effects of a glucose and amino acid mixture prescribed for parenteral alimentation on ant-Candida activity of neutrophils were then examined. More than 1.0% of glucose inhibited the anti-Candida activity of human neutrophils in a dose-dependent manner. This glucose effect was reduced by the addition of the amino acid mixture (especially cystein solution) which is clinically prescribed with a carbohydrate mixture solution in Japan. These results suggest that the amino acid mixture may act as a neutralizre of the suppressive action of glucose for anti-Candida activity of neutrophils.
The direct effect of mastification on the immunity of mice prohibited oral alimentation could not be determined, since parenteral alimentation of these animals is very difficult. We examined instead the effects of components of saliva, the secretion of which is a main physiological response induced by mastification. A saliva component.lactoferrin augmented anti-Candaida activity of murine neutrophils. We observed that not only lactoferrin but also lysozyme inhibited the Candida growth in vitro synergistically with antifungal azoles. Oral administration of lactoferrin protected mice from lethal systemic infection by C.albicans. These findings suggest that mastification may augment the host-defense mechanisms against Candida infection perhaps through enhancement of secretion of saliva.
