Dec. 2013

Aug. 2007

Acrylamide in dog food
Kazutoshi Sugita; Junpei Yamamoto; Kimika Kaneshima; Chika Kitaoka-Saito; Masashi Sekimoto; Osamu Endo; Yukihiko Takagi; Yuko Kato-Yoshinaga
Fundamental Toxicological Sciences, May 2021, [Reviewed]

Age-dependent aggravation of oral malodor and periodontal disease in dogs
Naoki Iwashita; Kazutoshi Sugita; Sayaka Murata; Keiko Ichimura; Mitsuyuki Shirai; Masaharu Hisasue; Miyoko Saito; Takuma Aoki; Yukihiko Takagi; Fumitoshi Asai
Fundamental Toxicological Sciences, 04 Apr. 2019

Evaluation of the genotoxicity of PM2.5 collected by a high-volume air sampler with impactor　　　　　　　　　　　　　　　
K. Sugita; Y. Kin; M. Yagishita; F. Ikemori; K. Kumagai; T. Ohara; M. Kinoshita; K. Nishimura; Y.Takagi; D. Nakajima
Genes and Environ., Feb. 2019, [Reviewed]

Application of a portable gas chromatograph for quantitative measurement of canine oral malodor
N. Iwashita; K. Sugita; M. Shirai; S. Murata; S. Yanagisawa; S. Goto; Y. Takagi; F. Asai
Fundam. Toxicol. Sci., 2017, [Reviewed]

Establishing conditions for the storage and elution of rabies virus RNA using FTA (R) cards
T. Sakai; A. Ishii; T.. Segawa; Y. Takagi; Y. Kobayashi; T. Itou
J.Vet.Med.Sci., Apr. 2015, [Reviewed]

国産たばこ主流煙中特異的ニトロソアミン類の異なる捕集法を用いた測定　　　　　　　　　　　　　　　
May 2012, [Reviewed]

相模原市の表層土砂の変異原性
S. Hisamatsu; M. Hiyama; K. Kaneshima; D. Nakajima; S. Goto; Y. Takagi; S. Sonoki; O.Endo
J. Environ. Chem.,22(3) 83-88, 2012, [Reviewed]
As part of research on the method of collecting soil samples for mutagenicity measurement, a map of the former Sagamihara City was divided into a square mesh by drawing lines along every latitudinal minute and longitudinal minute. 40 surface soil samples were collected at the intersection points on the map, and the mutagenicity of the samples was examined by the Ames preincubation method using Salmonella typhimurium TA98 and TA100 strains. It was found that the average mutagenicity was 47 rev/g of soil when TA98 was used without S9mix, 87 rev/g of soil when TA98 was used with S9mix, 11 rev/g of soil when TA100 was used without S9mix, and 152 rev/g of soil when TA100 was used with S9mix, all of which were lower than the values reported in past documents, and were approximately the same as values obtained in the countryside. To provide an overview, the geographical distribution of specific mutagenic activity was examined. As a result, mutagenic substances easily detectable with TA98 strain (inducing frame shift type mutations) were found mostly in the center of the former Sagamihara City, whereas mutagenic substances easily detectable with TA100 strain (inducing base pair substitution type mutations) were found to have spread over the whole of Sagamihara City. In order to look for hot spots, and in order to understand the distribution of mutagenicity, a sampling plan using non-arbitrary selection of sites as in the present study was effective.

Mutagenicity of the Chlorination Reactant of Cow Dung and Horse Dung
Y. Takagi; S.Hisamatsu; Y.Maekawa; D.Nakajima; S.Kageyama; S.Goto
J. Environ. Chem., 2011, [Reviewed]
In the present study, the mutagenicity of chlorinated cow dung was measured by the Ames preincubation method using S. typhimurium TA100 and the TA98 strain. It was found that chlorination might induce mutagenicity in cow dung, and it could be detected without S9mix. Analysis revealed that the most reliable conditions for analyzing this effect, might be the use of extracts of dung chlorinated at 20°C for 2 hours with 6% chlorine solution. When the mutagenicity of five samples of chlorinated cow dung and three samples of chlorinated horse dung were measured under these conditions, there was no difference in mutagenicity between individual cows and individual horses. In general, mutagenicity sensitivity of the TA100 strain tended to be higher than that of the TA98 strain.

Application of Ultramicro Forward-Mutation Assay to the Monitoring of Indoor and Outdoor Air Mutagenicity-Ezamples of Chengdu City and Tokyo,　　　　　　　　　　　　　　　
Y. Takagi; D. Nakajima; S. Chengjun; S. GotoO
2011, [Reviewed]

Mutagenicity and PAH Contents of Soil in Forests or Planted Areas in Japan
S. Goto; D. Nagaosa; S. Kageyama; D. Nakajima; S. Mineki; T. Yamamura; O. Endo; K.Kohzaki; Y. Takagi
Bull. Environ. Contam. Toxcol., Nov. 2009, [Reviewed]

Mutagenicity of Surface Sand in Park Sandboxes
Y. TAKAGI; D.NAKAJIMA; Y. KATO; T.A. OKATANI; K. KOHZAKI; K. INABA; S. MINEKI; S. GOTO
J. Environ. Chem., Jun. 2008, [Reviewed]
Fine sand of less than 60 mesh in size collected from the sandboxes of 40 urban parks throughout the country was subjected to methanol extraction. The mutagenicity of the extract was then measured by the Ames (pre-incubation) method. Most of the samples exhibited mutagenicity, which varied in severity, by four orders of magnitude from the lowest to the highest. The study also indicated that mutagens such as those detected in the TA98 or YG1024 strain existed. High mutagenicity was observed under the condition where S9mix was not added, which suggested that contamination due to direct mutagens was worsening in the sandboxes in parks. By region, mutagenicity tended to be higher in highly populated regions with much industrial activity such as the Kanto area.

Recovery Rate in the Concentration of Semivolatile Polycycric Aromatic Hydrocarbon(PAH) Solutions.
S.Mineki,Y. Kawakami; D.Nakajima; T; Shiozaki; K. Sugita; F. Shiraishi; Y.Takagi; S. Goto
J. Environ. Chem.,, Apr. 2008, [Reviewed]
Dichloromethane, acetone, and methanol solutions containing semivolatile polycyclic aromatic hydrocarbons with 2 to 6 rings were concentrated using rotary and centrifugal evaporators, and spraying nitrogen, and their recovery rates were compared. With concentration using a rotary evaporator, the recovery rate of low-molecular-weight PAHs was found to be low, but by adjusting the pressure in accordance with the volatilization pressure of the solvent, an almost uniform recovery rate was achieved. When a centrifugal evaporator was used, the recovery rate was approximately 60 %. However, since high-molecular-weight PAHs tended to attach to the internal wall of the vessels used, washing was carried out by applying ultrasonic waves, which improved the recovery rate. With concentration based on nitrogen spraying, an almost uniform recovery rate was achieved.

MEASUREMENT OF CONCENTRATIONS OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN SANDBOXES IN 51 JAPANESE PARKS
Y Takagi; S Mineki; N Sera; D Nakajima; A T Okatani; Y Kato; K Kohzaki; S Hisamatsu; S Goto
POLYCYCLIC AROMATIC COMPOUNDS, 2008, [Reviewed]

Volatile Organic Compound (VOC) analysis and anti-VOC measures in water-based paints
H.Nakashima; D.Nakajima; Y. Takagi; S.Goto
J. Health Sci., Jun. 2007, [Reviewed]

Gas Chromatography-mass Spectrometric Determination of Ergosterol as a Chemical Marker for Fungal Biomass of Indoor and Outdoor Air by Large-volume Sample Injection
ONJI Yoshiki; TAKEDA Kouzou; MATSUURA Hirofumi; HAGA Satomi; NAKAJIMA Daisuke; TAKAGI Yukihiko; GOTO Sumio
JEC, 2007
Large-volume sample injection technique was utilized for gas chromatography-mass spectrometric determination of ergosterol to estimate fungal biomass of indoor and outdoor air. Sample was collected with a low volume air sampler onto a filer paper for 24 hours and saponified in methanolic KOH. Ergosterol was extracted with n-hexane and then derivatized to its TMS ether. The ergosterol-TMS ether was stable for a month in a refrigerator.
The measurements of ergosterol in the samples were carried out by sample injection of 50 μl using a large volume injector into GC/MS followed by SIM analysis using ions m/z 363, 337 and 468. The lowest detectable amounts of the TMS derivative of ergosterol was 5 pg (signal-to-noise ratio of 3, m/z 363).
Actual air samples had been collected monthly in Nara city from January to December throughout 2006. The samples of indoor and outdoor air contained ergosterol from 0.03 to 0.96 ng/m³ and from 0.04 to 0.49 ng/m³, respectively.

陰地義樹，武田耕三，松浦洋文，芳賀敏実，髙木敬彦,中島大介，後藤純雄　　　　　　　　　　　　　　　
2007, [Reviewed]

深度１ｍまでの土壌の抽出物の変異原性　　　　　　　　　　　　　　　
2007, [Reviewed]

Anti-mutagenic and anti-oxidative activities found in Chinese traditional soybean fermented products furu
HF Ren; HE Liu; H Endo; Y Takagi; T Hayashi
Food Chem., Mar. 2006, [Reviewed]

Investigation of Mutagenicity of Vegetable Soil Amendments
影山志保; 中島大介; 山本貴士; 峯木茂; 遠藤治; 高木敬彦; 光崎研一; 後藤純雄
環境化学, 2006, [Reviewed]

Detection mutagenicity by Ames Preincubation method of fine commercial particles.　　　　　　　　　　　　　　　
Mineki S; Kobayashi A; Kageyama S; Nakajima D; Shiraishi F; Takagi Y; Onodera S; Takeda K; Goto S
Dec. 2005, [Reviewed]

Measurement of concentrations of polycyclic aromatic hydrocarbons and dioxin compounds in canine lungs
Y Takagi; D Nakajima; S Goto; K Sugita; K Uchida; T Matsumura; Y Kato; K Kohzaki
POLYCYCLIC AROMATIC COMPOUNDS, Nov. 2005, [Reviewed]

Effects of organic solvents in luminescent umu test using S. typhimurium TL210.
D.Nakajima; R.Ishii; K.Nishimura; Y.Takagi; S.Mineki; S.Onodera; S.Goto
JEC, 2005, [Reviewed]
We evaluated the effects of 15 different organic solvents in the luminescentumutest, a simplified version of the conventionalumutest, by determining the amount of luminescence induced by each solvent and considering its toxicity and stability of the solvents with regard to the following 15 types of solvents: methanol, dimethyl sulfoxide, acetonitrile, 1, 4-dioxane, tetrahydrofuran, acetone, cyclohexane, ethyl alcohol, N, N-dimethylformamide, ethylene glycol monophenyl ether, ethylene glycol dimethyl ether, glycerol formal, 1-methyl-2-pyrolidone, tetrahydrofurfuryl alcohol, and formamide. We found that cyclohexane, methanol, acetonitrile, ethanol, and acetone could be used in the test. Methanol, in particular, was effective for determining the mutagenicity of environmental samples.

Detection of mutagenicities of chlorinated toluenes and related compounds using a gas-exposure apparatus　　　　　　　　　　　　　　　
S. Mineki; Y. Takagi; A. Takahashi; S. Goto; D. Nakajima; O. Endo. H.Matsushita
Mater. Technol., 2005, [Reviewed]

Effects of heating and the addition of seasonings on the anti-mutagenic and anti-oxidative activities of polyphenols
H Bao; HF Ren; H Endo; Y Takagi; T Hayashi
Food Chem., Aug. 2004, [Reviewed]

Measurement of polynuclear aromatic hydrocarbons in canine lung after alkaline decomposition
Y Takagi; K Sugita; M Muto; Y Kato; KI Kohzaki; O Endo; S Goto
J.Vet.Med.Sci., Jul. 2004, [Reviewed]

有機性廃棄物を主原料とする堆肥抽出物の変異原性　　　　　　　　　　　　　　　
2004, [Reviewed]

Mutagenicity of Extracts from Size Fractionated Soils
TAKAGI Yukihiko; KITANI Ryouhei; KATO Yukio; KOHZAKI Ken-ichi; ENDO Osamu; MINEKI Shigeru; NAKAJIMA Daisuke; KAGEYAMA Shiho; GOTO Sumio
JEC, 2004, [Reviewed]
The surface soils were collected from 10 roadside sites in Tokyo bay area, and were classified into 5 sizes. The mutagenicity of methanol extracts of those samples were measured by pre-incubation method usingSalmonella typhimuriumstrains. The soil extracts showed strong mutagenicity toward YG1024 strain especially with S9mix, suggesting that the soil extracts contain higher amounts of pro-mutagen than direct mutagen. Relatively higher mutagenicities were observed from 100-212μm and 53-100μm fraction than other fractions.

Mutagenic activities and PAH concentrations in indoor and outdoor air in chengdu, China　　　　　　　　　　　　　　　
Y.Takagi; K.Sugita; D.Nakazima; S.Chengjun; M.Koyano; O.Endo; S.Goto
2004

A modification of the luminescent umu test in an S9 activation system
D.Nakajima; K.Nishimura; R.Ishii; Y.Takagi; K.Tanada; S.Mineki; S.Goto
Proceedings of China-Japan Joint Symposium on Environmental Chemistry, October 21-23, Beijing, China, 2004, 2004

Mutagenicity of suspended particulate matter divided in three sizes indoors
Y Takagi; S Goto; D Nakajima; O Endo; M Koyano; K Kohzaki; H Matsushita
J. HEALTH SCI., Dec. 2002, [Reviewed]

Mutagenicity and PAH concentration of soil collected from urban area of Tokyo
S.Mineki; S.GotoO.Endo; C.Sun; Y.Takagi; K.Kohzaki; S.Onodera
Archives Complex Environ. Stud., 2001, [Reviewed]

Reseach for the recent increase of roof rat infection in residential areas in Tokyo
T.Yabe; R.Asai; Y.Takagi,K.Kohzaki
Med. Entomol. Zool., 2000, [Reviewed]
We surmised that the dispersal of roof rats, Rattus rattus, from commercial areas was the primary cause of the recent increase of rat infestation in residential areas in the center of Tokyo. Questionnaires received from 322 residences randomly chosen in Shinagawa Ward proved that roof rats had invaded 75 (23.3%) of them within the past two years. Detached houses with roof rat infestation were significantly closer to commercial areas than those without rodent invasion (P=0.016).

Evaluation of the Solid-phase Extraction Disks for Ames Mutagenicity Test in River Water
MORI Yasuaki; SETSUDA Setsuko; TAKAGI Yukihiko; KOHZAKI Ken-ichi; MURAKAMI Kazuo; GOTO Sumio; ENDOH Osamu; ONODERA Sukeo
JEC, 2000, [Reviewed]
A comparison of the solid-phase extraction disks and the solid-phase extraction cartridges for Ames mutagenicity test of river water was conducted. Acetone and ethyl acetate were suitable solvents for extracting mutagen from the disk and the cartridge after the passage of river water.
Mutagenicity, towards test strain S. typhimurium TA98, of the extracts obtained from disks was almost similar to that of the extracts obtained from cartridges. Thus, the use of solidphase extraction disk was useful as an extraction procedure for the Ames mutagenicity test of river water. This extraction procedure was applied to seven rivers in Kanagawa Prefecture and their Ames mutagenic activities were evaluated.

有機無農薬栽培による野菜類抗変異原性の変化
任 恵峰; 高木敬彦; 包航; 後藤純雄; 遠藤英明; 林 哲仁
日本食品科学工学会誌, 2000, [Reviewed]
Many reports on anti-mutagenicity have been made with vegetables cultivated by an ordinary manner (OV). This paper presents the results of anti-mutagenicity study of organically cultivated vegetables (OCV), using water soluble chitosan for soil improvement and leaf surface spray, in comparison with that of OV. Their anti-mutagenicity were evaluated by the forward mutation assay using Salmonella typhimurium TM677. A clear difference between the juices prepared from OCV and OV was observed in the ability to inhibit the mutagenicity of authentic mutagenic compounds, 4NQO, BaP, and Trp-P-2.

S.GOTO, K.SUGITA, Y.TAKAGI, K.KOHZAKI, O.ENDO, H.YAJIMA, T.ISHII, H.MATSUSHITA　　　　　　　　　　　　　　　
S.Goto; K.Sugita; Y.Takagi; K.Kohzaki; O.Endo; H.Yajima; T.Ishii; H.Matsushita
Indoor Air, 1999, [Reviewed]

Inorganic components in dokudami (Hulluynis cordala) tea at different growth stages
Kohzaki Ryuko; Sakaguti Kazuko; Kohzaki Kenichi; Takagi Yukihiko; Mori Mayumi; Suzuki Keiko
Journal of Cookery Science of Japan, 1999, [Reviewed]
The appropriate picking time for dokudami (Huttuynia cordata) tea is generally considered to be at the termination of the flower-like white involucre. Therefore, we classified the growth period of dokudami into the sprouting, seed setting, and true leaf stages, analyzed the contents of inorganic components at these stages, and analyzed contents of inorganic components in the leaves and flowers in the seed setting stage. In addition, the importance of the tea picking time was evaluated by a correlation analysis and principle component analysis.
Among the inorganic components of dokudam i, the content of potassium was the highest, and the contens of calcium, magnesium, iron, manganese and zinc increased with growth. The content of copper was high in the flowers after diplophase parthenogenesis.
Inorganic components increased at a potassium: calciu m ratio of about 6: 1 and at a manganese: magnesium ratio of about 80: 1. The calcium: potassium ratio changed inversely to the copper content that was observed in the flowers and in the true leaf stage.
A principle component analysis revealed calcium, magnesium, potassium, manganese, and copper as the principle components.
These appeared to be the principle inorganic components of dokudami and to increase due to diplophase parthenogenesis. A principle component analysis a ccording to the growth stage showed that the specificity of flowers could be estimated from two-dimensional scatter diagrams. The appropriate picking time for dokudami tea is empirically considered to be at the termination of the involucre, and the analysis according to the growth stage and the analysis of the flowers and leaves confirmed this. To improve the abnormal gustatory that is sensation associated with a modern diet or with insufficient intake of inorganic components by aged persons, dokudami tea brewed from a herbal plant provides a readily drunk remedy.

Detection of Salmonella and Campylobacter from Rats Trapped at Restaurants and a Fish Market
KATO Yukio; NAKAI Yasuhiro; MATSUSHITA Maki; TAKAGI Yukihiko; KOHZAKI Ken-ichi; KANEUCHI Choji
Journal of the Japan Veterinary Medical Association, 1999, [Reviewed]

Mutagenicity of dog urine determined by blue rayon extraction and the ultramicro forward-mutation method
Y Takagi; H Sasaki; O Endo; M Koyano; S Goto; Y Kato; C Kaneuchi; K Kohzaki
J.Vet.Med.Sci., Nov. 1997, [Reviewed]

Method of determining the inhalation deposition of mutagenic components in environmental tobacco smoke aerosol
S Goto; K Sugita; O Endo; Y Takagi; H Matsushita; J Lewtas
J. TOXICOL. ENVIRON. HEALTH, Apr. 1997, [Reviewed]

空気中の変異原性物質の個人曝露に及ぼす喫煙及び受動喫煙の影響　　　　　　　　　　　　　　　
1997, [Reviewed]

Using Kerosene or Gas Heaters and Concentration of Polycyclic Aromatic Hydrocarbons in Indoor Air Particles
TAKAGI Yukihiko; KAN Masakazu; KOYANO Michiko; GOTO Sumio; KATO Yukio; KANEUCHI Choji; KOHZAKI Ken-ichi
Journal of the Japan Veterinary Medical Association, 1997, [Reviewed]

Mutagenicity of the Particular, Gaseous and Vaporus Substances of Cigarette Side-Stream
TAKAGI Yukihiko; TAKAHASHI Kiyoshi; ENDOH Osamu; GOTO Sumio; KOHZAKI Ken-ichi; MATSUSHITA Hidetsuru
Journal of the Japan Veterinary Medical Association, 20 Sep. 1996
Particular, gaseous and vaporus substances of cigarette side-stream smoke were investigated for mutagenicity by the Ames preincubation and gas exposure systems. Particulates were mutagenic for in theEscherichiacoli WP 2uvrA/pKM 101 andSalmonella typhimuriumTA 100 and TA 98, while gaseous and vaporus substances were not forS. typhimuriumTA 98. There was no difference in mutagenicity forS. typhimuriumTA 100 between gaseous or vaporus substances and particulates, but the mutagenicity of gaseous and vaporous substances forE. coliWP 2uvrA/pKM 101 was 5 to 10 times higher than that of particulate.

Mutagenicity of Resin-Absorbed Materials from Tap Water and Effects of Home Water Cleaners on Its Elimination
TAKAGI Yukihiko; UENO Miki; GOTO Sumio; KATO Yukio; KANEUCHI Choji; KOHZAKI Ken-ichi
Journal of the Japan Veterinary Medical Association, 1996
Mutagenicity of resin-absorbed materials from tap water and its elimination by home water cleaners were evaluted by Ames assay with and without S9mix. CSP800-or CHPA25-absorbed materials from tap water two cities examined showed positive mutagenic activities that have coefficient variations from 30 to 50% daily. The home water cleaners tested were shown to be useful for reducing mutagens in tap water.

Effects of indoor air pollution on personal exposure to direct mutagens　　　　　　　　　　　　　　　
S.Goto; O.Endoh; T.Okubo; Y.Takagi; H.Matsushita; R.W.Williams; J.Lewtas
Indoor Air, 1996, [Reviewed]

タバコ副流煙の粒子状およびガス・蒸気状物質の変異原性　　　　　　　　　　　　　　　
1996, [Reviewed]

Characterization of Staphylococcus aureus from Rats in a Fish Market and a Slaughterhouse
KATO Yukio; MATSUMOTO Satoshi; USHIODA Hiroshi; TANIKAWA Tsutomu; TAKAGI Yukihiko; KOHZAKI Kenichi; KANEUCHI Choji
Journal of the Japan Veterinary Medical Association, 1996, [Reviewed]
Staphylococcus aureus was isolated from 44 (74.6%) of 59 rats (Rattus norvegicus) from a fish market in Chiba Prefecture and 9 (34.6%) of 26 rats from a slaughterhouse in Kanagawa Prefecture. Of 104 isolates from the fish market, the most frequent was 81 strains (77.9%) of biotype C, followed by 16 strains (15.4%) of biotype B, 6 strains (5.8%) of biotype A and untypable 1 strain (1.0%). Eight (88.9%) of 9 isolates from the slaughterhouse were of biotype G and 1 (11.1%) was of biotype B. The coagulase types of isolates from the fish market were of VIII (26.9%), I (22.1%), VII (13.5%), IV (8.7%), II (5.8%), III (2.9%), V (2.9%), VI (2.9%) and untypable (14.4%), while those from the slaughterhouse were of VIII (44.4%), V (22.2%), VII (22.2%) and untypable (11.1%). Twelve isolates from 9 rats in the fish market produced enterotoxins A (11 strains) and B (1 strain).

樹脂吸着による水道水の変異原性と家庭用浄水器による除去効果　　　　　　　　　　　　　　　
1996, [Reviewed]

ジアミノジフェニルメタンおよびその関連化合物の変異原性　　　　　　　　　　　　　　　
1995, [Reviewed]

揮発性ニトロソアミンの変異原性測定法について　　　　　　　　　　　　　　　
1995, [Reviewed]

The Study on Exposure Factors to Airborne Mutagens
TAKAGI Yukihiko; HATANO Akira; KOHZAKI Ken-ichi; GOTO Sumio; MATSUSHITA Hidetsuru
Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi, 1994
The mutagenicities of both samples obtained by the personal samplers and the sanplers of airborne particulates in outdoors were compared, and the exposure-factors to mutagens were investigated. The personally exposed particulates were collected by means of the portable mini-pumps at a flow rate of ca. 1.5l/min for 24 hr, and the airborne particulates in outdoors were collected by a high volume air sampler at the same time. Four volunteers, two nonsmokers and smokers, were equipped with personal samplers, and were given the inqurity cards, which asked the numbers of cigarettes smoked, passive smoking and heating, during a week at each month of Feb., May, Jul. and Oct. in 1991. Organic components in airborne particulates were extracted by ultrasonication with dichlorometane. Mutagenic activities of these extracts were bioassayed for Salmonella typhimurium TM677 strain with and without S9mix using the ultramicro forward-mutation assay.
A significant correlation between the direct mutagenic activity levels and the number of cigarettes smoked was obtained from the personal data. From the comparison between the personal data of nonsmokers and the outdoor data, a good correlation was not obtained. The various factors such as heatings and poor ventilations enhanced the personal exposure level to airborne mutagens. These results suggest that the sampling and the bioassay technique are important to investigate, and evaluate the personal exposure to airborne mutagens.

首都圏のタ－ミナル駅地下街空気中の多環芳香族炭化水素濃度について　　　　　　　　　　　　　　　
1994, [Reviewed]

空気浮遊粒子中の変異原物質への曝露要因に関する研究-個人曝露レベルと屋外空 気汚染レベルとの比較-　　　　　　　　　　　　　　　
1994, [Reviewed]

Mutagenicity of airborne particulates in Sagamihara city
Y.Takagi; K.Sakiya; O.Endoh; S.Goto; K.Kohzaki; M.Murata; H.Matsushita
J.Vet.Med.Sci., 1992, [Reviewed]
Airborne particulates in Sagamihara city were collected on quartz fibre filters using a high-volume air sampler for 14 days in each month from July 1984 to June 1985. Organic components in airborne particulates were extracted by the ultrasonic extraction method using benzene-ethanol(3:1 v/v) as an extracting solvent. The mutagenicities of airborne particulates extract were measured by the pre-incubation method using Salmonella typhirnurium strains TA100 and TA98 with and without S-9mix. The concentration of airborne particulates was nearly the same level throughout the survey period, but the content of extract in particulates of winter was higher than those of other seasons. All the airborne partiuclates extracts showed positive mutagenic response to both strains with and without S-9mix. The average of mutagenic activities (revertants/m^3 air) in winter was significantly higher than those in summer and spring. Furthermore,mutagenic activities fluctuated sharply in one month from several to ten times compared with the normal level, depending upon sampling days, and tended to be lower on Sundays and holidays in summer and new year holidays. In many days mutagenic activities without S-9mix were comparatively higher than those with S-9mix. The existence of nitroarene was surveyed using TA98NR strain. Nitroarene was found to be higher concentration in summer than in winter.

Biological and chemical methodologies for assessing human exposure to airborne mutagens indoors　　　　　　　　　　　　　　　
H.Matsushita; S.Goto; O.Endoh; K.Tanabe; M.Koyano; Y.Takagi; M.Murata; J.Lewtas
Indoor Air, 1990, [Reviewed]

Highly sensitive bioassays for evaluating airborne mutagens indoors　　　　　　　　　　　　　　　
S.GotoY.Takagi; O.Endoh; J.Lewtas; H.Matsushita
Indoor Air, 1990, [Reviewed]

HUMAN EXPOSURE TO AIRBORNE MUTAGENS INDOORS AND OUTDOORS USING MUTAGENESIS AND CHEMICAL-ANALYSIS METHODS
H Matsushita; S Goto; Y Takagi; O Endo; K Tanabe
GENETIC TOXICOLOGY OF COMPLEX MIXTURES, 1990

Ultramicro Forward-mutation Assay and It's Application to the Survey of Indoor Air Pollution
TAKAGI Yukihiko; GOTO Sumio; KUO Ching-Tang; SUGITA Sawako; MURATA Motohide; LEWTAS Joellen; MATSUSHITA Hidetsuru
Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi, 1989, [Reviewed]
A highly sensitive ultramicro forward-mutation assay using Salmonella typhimurium TM677 was achieved by the introduction of micro-vessel in the preincubation step. This assay was about 10 times higher in sensitivity than the micro forward-mutation assay and was able to measure mutagenicity of extracts from airborne particulates obtained by only 3m³ air sampling. Repeatability of this assay was nearly same with that of the micro forward-mutation assay, that is, the coefficient of variation of the mutation frequency for airborne particulate extracts was 12.3% in the test condition without S9 mix and 13.7% with S9 mix. This assay was applied to the measurement of hourly variation mutagenic activity of airborne particulates indoors and outdoors, and revealed that mutagenic activity of indoor air was generally higher than that of outdoor air and that the mutagenic activity indoors in the absence of S9 mix was well correlated with human activity.
It was also found by this assay and PAH analysis that indoor pollution by carcinogens and mutagens was largely affected by cigarette smoking and an air cleaner was useful for the reduction of indoor pollution by mutagens and PAHs.

Application of the Micro-forward Mutation Assay to Asses Mutagenicity of Airborne Particulates in Indoor
TAKAGI Yukihiko; GOTO Sumio; MURATA Motohide; MATSUSHITA Hidetsuru; LEWTAS Joellen
Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi, 1988
Validity test of the micro-forward mutation assay using Salmonella typhimurium strain TM677 was carried out using benzene-ethanol extracts from airborne particulates as test materials. Sensitivity of this assay in the presence and absence of S-9mix was five to ten times higher than the pre-incubation method, a modified method of Ames's original method, using Salmonella typhimurium strains TA98 and TA100. This means that the virtual sensitivity of the micro-forward mutation assay is ten times or more higher than the pre-incubation method, because two or more strains are necessary in the latter method. Repeatability of the micro-forward mutation assay was nearly same with that of the pre-incubation method. That is, the coefficient of variation of mutagenic frequency of the particulate extracts was about 10%.
This forward mutation assay was applied to airborne particulates indoors, and proved to be able to measure easily the mutagenic activity of the extracts from particulates collected by only 30m³-air sampling. Some indoor pollutants, especially ones collected in a room in which cigarette smoking was done, showed higher mutagenic activity than that of outdoor sample. Particle size dependency of the mutagenic activity could also measure by this method using air particulates collected by only 90m³-air sampling with an Andersen/Low Pressure Impactor. The highest mutagenic activity was found in the particulates of 0.52-0.33μm in size. These results suggest strongly that the micro-forward mutation assay is a useful tool for survey mutagenicity of indoor environment.

Mutagenicities of Engine Oils of Gasoline and Diesel Cars
TAKAGI Yukihiko; ENDOH Osamu; GOTO Sumio; KAWAI Akihiro; MURATA Motohide; MATSUSHITA Hidetsuru
Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi, 1986, [Reviewed]
Effective method for extracting mutagens in engine oils was investigated in order to determination emission level of mutagens from gasoline and diesel cars, mutagenic activity in engine oil was attended and a effective method for extracting chemicals was investigated. Engine oils were collected from 12 gasoline which were driving in Tokyo, and 7 diesel cars which were driving outside of Tokyo. Engine oils from mileage were also collected for every 1, 000 km after filling with new oils. The pre-incubation modified method of Ames original method was used for estimating mutagenicity using Saimonella typhimurium strains TA100 and TA98 with and without S9mix.
The combination of the reflux methanol extraction and liquid-liquid extraction using isopentane and acetonitrile was effective for detecting mutagens in both gasoline and diesel engine oils. Relationship between the mutagenicity and the mileage was observed towards both types of cars. Mutagenic activities of gasoline engine oils showed generally higher values than those of diesel engine oils. Mutagenicities of gasoline engine oils from longer distance driving cars more than 30, 000km were also higher values than shoter distance driving cars.

トリニトロクロルベンゼンおよびテトラニトロカルバゾ－ルの変異原性に及ぼすピ レンおよびBaPの影響　　　　　　　　　　　　　　　
1984, [Reviewed]

Beneficial Effect of Water-soluble Silicon on the Meat Quality and Fecal Odor of Broiler Chickens
Apr. 2017

Effect of Water-soluble Silicon on Liver Damage and Fecal Odor in High-fat Diet-fed Mice　　　　　　　　　　　　　　　
Jun. 2015

Test Method for Fungi(9)The Analysis of Mold Odors
Apr. 2015

国内数地点で採取したPM2.5の変異原性
2015

福島第一原発事故前後における相模原市内の表層土砂のセシウム濃度比較
坂野英知; 久松伸; 兼島公香; 高木敬彦; 伊藤彰英; 郭錦堂; 影山志保; 諸岡信久; 後藤純雄
麻布大学雑誌, 31 Mar. 2014

相模原市内の土砂のセシウム汚染レベル
2013

建材中石綿之χ光繞射儀分析方法動態　　　　　　　　　　　　　　　
Dec. 2012

牛糞および馬糞の塩素化反応物の変異原性
2009

数種のナノ製品の変異原性について
2008

Testicular toxicology of rats prenatally exposure to 3,3',4,4',5-pentachlorobiphenyl
Journal of Azabu University, 2008
Azabu University

P-127 Mutagenicity and PAH Content of Forest or Planting Soil(Poster Session)
Goto Sumio; Nagaosa Daisuke; Kageyama Shiho; Nakajima Daisuke; Shiraishi Fujio; Mineki Shigeru; Yamamura Takaki; Endo Osamu; Kohzaki Ken-ichi; Takagi Yukihiko
2008

土壌汚染の実態把握のための評価方法　　　　　　　　　　　　　　　
2008

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Mar. 2007

大気中の粒子状及びガス状変異原の測定
2007

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Mar. 2006

雑木林土壌試料の変異原性について
2006

小動物獣医看護学　　　　　　　　　　　　　　　
Nov. 2005

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Mar. 2005

ニトロフミン酸抽出物の変異原性について
2005

P-89 Mutagenicity of soils collected at children's parks in Kawasaki(Poster Presentations(2))
Endo O; Koyano M; Nakajima D; Goto S; Watanabe T; Takagi Y; Kohzaki K
2005

発がん性物質暴露に対する肝細胞の初期反応に関する毒性病理学的検討　　　　　　　　　　　　　　　
Mar. 2004

P2-068 Effect of organic solvents with the luminescent umu test using S.typhimurium TL210
Goto Sumio; Nakajima Daisuke; Ishii Ruri; Endo Osamu; Takagi Yukihiko; Onodear Sukeo
2004

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Jan. 2004

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Mar. 2003

土壌変異原性測定時におけるクロロフィル類の影響について
2003

粒径別に分けた土砂試料の変異原性
2003

生物評価試験による浮遊粒子状物質の長期曝露モニタリングに関する研究　　　　　　　　　　　　　　　
Mar. 2002

In-utero and lactational exposure of 3, 3"4, 4', 5-pentachlorobiphenyl modulate dimethlbenz [a] anthracene-induced rat mammary carcinogenesis.
Wakui Shin; Takagi Yukihiko
Journal of Azabu University, 2002
While polychorinated biphenyls (PCB) are fat-soluble environmental pollutants stored breast fatty tissue and secreted in milk;the precise evidence of breast carcinoma from exposure to PCBs remains unclear. Then we investigated the interrelation between the expression of mammary tumor cytochrome P450 1B1 (CYP1B1) and the dose-response relationship in-utero and lactational exposure of PCBs congener for dimethlbenz[a]anthracene (DMBA)-induced rat mammary carcinogenesis. Female SD rats were injected (i.g.) with 25 pg, 2.5 ng, 250 ng, 7.5 ug of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB126)/kg, or the vehicle, on days 13 to 19 post-conception. Fifty-day-old female offspring were injected (i.g.) with 20mg DMBA/kg. The expression of CYP1B1 in the mammary tumors was intensive in the 250 ng-and 2.5 ng-groups, and slight in the 7.5 ug-group, and not observed in the other groups. The present studies indicate that in-utero and lactational exposure of relatively low dose PCB reveal high level CYPIBI which metabolize the 17beta-estradiol into the 3, 4-catecholestradiol acts as a enhancing agent toward DMBA-induced rat mammary carcinogenesis., Azabu University

Mutagenicity monitoring of soils in parks.
GOTO Sumio; NAKAJIMA Daisuke; KAGEYAMA Shiho; EZOE Yuka; ENDO Osamu; TAKAGI Yukihiko; Morooka Nobuhisa; ONODERA Sukeo
2002

Evaluation of the Solid-phase Extraction Disks for Ames Mutagenicity Test in River Water
MORI Yasuaki; SETSUDA Setsuko; TAKAGI Yukihiko; KOHZAKI Ken-ichi; MURAKAMI Kazuo; GOTO Sumio; ENDOH Osamu; ONODERA Sukeo
JEC, 2000
A comparison of the solid-phase extraction disks and the solid-phase extraction cartridges for Ames mutagenicity test of river water was conducted. Acetone and ethyl acetate were suitable solvents for extracting mutagen from the disk and the cartridge after the passage of river water.
Mutagenicity, towards test strain S. typhimurium TA98, of the extracts obtained from disks was almost similar to that of the extracts obtained from cartridges. Thus, the use of solidphase extraction disk was useful as an extraction procedure for the Ames mutagenicity test of river water. This extraction procedure was applied to seven rivers in Kanagawa Prefecture and their Ames mutagenic activities were evaluated., Japan Society for Environmental Chemistry

Study on inhibition of intercellular communication by airborne particle extracts
UTSUNOMIYA Kimiko; MACHII Kenji; GOTO Sumio; ENDO Osamu; MATSUKI Sachiyo; FUKAI Fumio; TAKAGI Yukihiko; KOZAKI Kenichi; KATOH Motoe
1999

Study on detection method of mutagenicity of dog urine samples
TAKAGI Yukihiko; HIRAI Noriko; KOZAKI Kenichi; GOTO Sumio; ENDO Osamu; MORI Yasuaki
1999

Mutagenicity of dog urine determined by blue rayon extraction and the ultramicro forward-mutation method (vol 59, pg 1082, 1997)
Y Takagi; H Sasaki; O Endo; M Koyano; S Goto; Y Kato; C Kaneuchi; K Kohzaki
JOURNAL OF VETERINARY MEDICAL SCIENCE, Jan. 1998

P-16 マイクロサスペンジョン法におけるプレインキュベーション条件の検討
1998

Mutagenicity Detection of Volatile N-Nitrosamines
Kenji MACHII; Hiroaki MOCHIZUKI; Yukihiko TAKAGI; Akihiro KAWAI; Sumio GOTO; Osamu ENDO; Shigeru MINEKI; Hidetsuru MATSUSHITA
Journal of Environmental Chemistry, 1995
A new gas exposure system that measures the mutagenicity of volatile nitrosamines was evaluated. The examinations used to determine the test conditions in this system, using N-nitrosodiethylamine (NDEA) under S9mix conditions, showed the greatest mutagenicity in theE. coliWP2uvrA/pKM101 strain, next in theS. typhimuriumTA100 strain, and no mutagenicity in theS. typhimuriumTA98 strain. The variation coefficients obtained from this system were shown to be comparatively small (3.9-10.8%) .
The mutagenic activity of the four nitroso compounds were compared in three assays using the WP2uvrA/pKM101 strain with the S9mix. The order of mutagenic activity obtained was as follows: N-nitrosodi-n-butylamine>N-nitrosodi-n-propylamine > N-nitrosodiethylamine>N-nitrosodimethylamine. The mutagenic detectability with increasing molecular weight of the nitrosamines was as follows: gas exposure system>preincubation assay>plate incorporation assay., Japan Society for Environmental Chemistry

空気中の微量有害物質への経気道個人曝露調査手法の開発に関する研究　　　　　　　　　　　　　　　
1993

空気中の微量有害物質への経気道個人曝露調査手法の開発に関する研究　　　　　　　　　　　　　　　
1993

生存競争モデルによる伝染病等の流行に関する理論疫学的研究
1991

前進突然変異試験法による環境変異原物質の高感度検出　　　　　　　　　　　　　　　
1988

室内空気の質の制御に関する物理・化学的研究　　　　　　　　　　　　　　　
1987

室内空気の質の制御に関する物理・化学的研究 －室内空気質の生物学的評価手法の 開発に関する研究－　　　　　　　　　　　　　　　
1987

MUTAGENICITY AND CHEMICAL-STRUCTURE OF AROMATIC DIAMINO COMPOUNDS
H MATSUSHITA; O ENDO; S GOTO; Y TAKAGI; M MURATA
MUTATION RESEARCH, 1985

MUTAGENICITY AND CHEMICAL-STRUCTURE OF DIAMINO AROMATIC-COMPOUNDS
O ENDO; Y TAKAGI; M MURATA; S GOTO; H MATSUSHITA
MUTATION RESEARCH, 1984

Measurement of halitosis components of dogs　　　　　　　　　　　　　　　
09 Sep. 2017

Furan in Dog food　　　　　　　　　　　　　　　
09 Sep. 2017

国産たばこ11銘柄の主流煙に含まれるフェノ－ル類7成分の分析　　　　　　　　　　　　　　　
01 Sep. 2017

国内数地点で採取したPM2.5の変異原性　　　　　　　　　　　　　　　
27 Nov. 2015

犬・猫及び爬虫類用ペットフード中の重金属 濃度の比較　　　　　　　　　　　　　　　
24 Jun. 2015

消毒剤散布が及ぼす土壌環境への影響　　　　　　　　　　　　　　　
24 Jun. 2015

室内空気中のMVOCの汚染実態　　　　　　　　　　　　　　　
23 Jan. 2014

室内空気中のMVOCのGC/MS分析と判定事例　　　　　　　　　　　　　　　
05 Dec. 2013

相模原市内の土砂のセシウム汚染レベル　　　　　　　　　　　　　　　
31 Jul. 2013

室内空気中の微生物由来揮発性有機化合物の存在実態　　　　　　　　　　　　　　　
31 Jul. 2013

ガムたばこの変異原性　　　　　　　　　　　　　　　
28 Mar. 2012

尿中酸化ストレスマーカーの測定-家庭飼育犬の尿中濃度-　　　　　　　　　　　　　　　
16 Jul. 2011

尿中酸化ストレスマーカーを指標としたイヌの健康評価法の検討　　　　　　　　　　　　　　　
16 Sep. 2010

1980年から2000年における都内大気浮遊粉じん中のダイオキシン類及びPAHｓ濃度トレンド　　　　　　　　　　　　　　　
02 Sep. 2010

ピレン資化性細菌Burkholderia sp. No. 7 株のピレン生育対数期に　　　　　　　　　　　　　　　
30 Mar. 2010

日本産たばこ主流煙のTobacco-specific Nitrosamines 測定法の検討　　　　　　　　　　　　　　　
28 Mar. 2010

健康なイヌの尿中酸化ストレスマ－カ－に関する基礎的研究　　　　　　　　　　　　　　　
26 Mar. 2010

牛糞および馬糞の塩素化反応物の変異原性　　　　　　　　　　　　　　　
09 Jun. 2009

神奈川県内の表層土壌の変異原性分布　　　　　　　　　　　　　　　
11 Jun. 2008

フィリピンにおける狂犬病抗体検査から日本の狂犬病対策再検討の　　　　　　　　　　　　　　　
25 Aug. 2007

Measurement of Concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) in Sandboxes in 51 Japanese Parks, polycyclic aromatic compounds(　　　　　　　　　　　　　　　
05 Aug. 2007

公園の砂場における砂の変異原性およびPAHｓ濃度　　　　　　　　　　　　　　　
20 Jun. 2007

イヌにおけるコアグラーゼ陽性ブドウ球菌保有状況の経時的調査　　　　　　　　　　　　　　　
22 Sep. 2006

我が国における狂犬病危機管理対策(４)フィリピンにおける狂犬病の診断とその後の対策について　　　　　　　　　　　　　　　
28 Aug. 2006

雑木材土壌試料の変異原性　　　　　　　　　　　　　　　
20 Jun. 2006

土壌深度1ｍまでの変異原性分布　　　　　　　　　　　　　　　
20 Jun. 2006

ドハトから分離されたStaphylococcus schleiferi subsp.coagulansについて　　　　　　　　　　　　　　　
29 Sep. 2005

Measurement of Concentrations of polycyclic aromatic hydrocarbons and dioxin compounds in canine lungs, polycyclic aromatic compounds　　　　　　　　　　　　　　　
21 Aug. 2005

ニトロフミン酸抽出物の変異原性について　　　　　　　　　　　　　　　
15 Jun. 2005

WITHIN-DAY VARIATIONS IN RESPONSE OF THE MOUSE BIOASSAY FOR PARALYTIC SHELLFISH POISONING TOXINS　　　　　　　　　　　　　　　
11 Apr. 2005

マウス購入時体重とその日内変動が,麻痺性貝毒のマウス試験における致死時間に与える影響の基礎的研究　　　　　　　　　　　　　　　
Nov. 2004

Mutagenic activities and PAH concentrations in indoor and outdoor air in chengdu, China　　　　　　　　　　　　　　　
20 Oct. 2004

A modification of the luminescent umu test in an S9 activation system　　　　　　　　　　　　　　　
20 Oct. 2004

ペットショップにおける子犬糞便中の病原細菌の分類　　　　　　　　　　　　　　　
Aug. 2004

土壌変異原性測定時におけるクロロフィル類の影響について　　　　　　　　　　　　　　　
07 Jul. 2004

犬におけるStaphylococcus intermediusおよび S.schleiferi subsp. Coagulansの保有状況　　　　　　　　　　　　　　　
03 Apr. 2004

粒径別に分けた土砂試料の変異原性　　　　　　　　　　　　　　　
26 Nov. 2003

マイクロサスペンジョン法とプレインキュベ－ション法による陽性対照試料の変異原性の比較　　　　　　　　　　　　　　　
26 Nov. 2003

土壌変異原性測定時におけるクロロフィル類の影響について　　　　　　　　　　　　　　　
25 Jun. 2003

公園の土壌の変異原性について　　　　　　　　　　　　　　　
27 Nov. 2002

発光umu細胞を用いる変異原性試験法の高感度化における留意点　　　　　　　　　　　　　　　
03 Jun. 2002

わが国の細菌性食中毒発生構造の検討 1)原因施設(飲食店)について　　　　　　　　　　　　　　　
31 Oct. 2001

児童公園内砂場の表層砂の変異原性について　　　　　　　　　　　　　　　
23 May 2001