
Kim Honsong
Faculty of Pharmaceutical Sciences,Department of Pharmaceutical Sciences | Lecturer |
Last Updated :2025/10/07
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Career
Educational Background
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Paper
- Ribosomal protein mutation suppresses gonadal leader cell migration defects in mig-17/ADAMTS mutants in Caenorhabditis elegans.
Hon-Song Kim; Kaito Mitsuzumi; Shohei Kondo; Rie Yamaoka; Shinji Ihara; Hiroshi Otsuka; Chizu Yoshikata; Yukihiko Kubota; Takumi Tomohiro; Toshinobu Fujiwara; Kenji Kimura; Fumio Motegi; Yukimasa Shibata; Mikiko Takahashi; Kiyoji Nishiwaki
Scientific reports, 21 Jul. 2025
The migration of gonadal distal tip cells (DTCs) in Caenorhabditis elegans serves as an excellent model for studying the migration of epithelial tubes during organogenesis. Mutations in the mig-17/ADAMTS gene cause misdirected DTC migration during gonad formation, resulting in deformed gonad arms. An amino acid substitution in RPL-20, the ortholog of mammalian RPL18a/eL20, a component of the 60 S ribosomal large subunit, exhibited a slow-growth phenotype and strongly suppressed the mig-17 gonadal defects. Slow-growing mutations clk-1 and clk-2 also suppressed mig-17. Intestine-specific overexpression of mutant RPL-20 protein resulted in a slow-growth phenotype and suppressed the mig-17 gonadal defects, but these effects were much weaker when wild-type RPL-20 was overexpressed, suggesting that the mutant RPL-20 protein acquired a novel function. Analysis of ribosome profiles revealed reduced biogenesis of the 60 S subunit, leading to a reduction of 80 S ribosomes in the rpl-20 mutant. These results suggest that DTC migration defects in mig-17/ADAMTS mutants can be partly suppressed by growth retardation caused by the rpl-20 mutation. While defective ribosome biogenesis may contribute to the observed growth retardation, further investigation is needed to clarify the molecular basis of this phenomenon. - Repulsive guidance molecule acts in axon branching in Caenorhabditis elegans
Kaname Tsutsui; Hon-Song Kim; Chizu Yoshikata; Kenji Kimura; Yukihiko Kubota; Yukimasa Shibata; Chenxi Tian; Jun Liu; Kiyoji Nishiwaki
Scientific Reports, 01 Dec. 2021 - Correction: Genetic interactions among ADAMTS metalloproteases and basement membrane molecules in cell migration in Caenorhabditis elegans.
Ayaka Imanishi; Yuma Aoki; Masaki Kakehi; Shunsuke Mori; Tomomi Takano; Yukihiko Kubota; Hon-Song Kim; Yukimasa Shibata; Kiyoji Nishiwaki
PloS one, 2021
[This corrects the article DOI: 10.1371/journal.pone.0240571.]. - Genetic interactions among ADAMTS metalloproteases and basement membrane molecules in cell migration in Caenorhabditis elegans.
Ayaka Imanishi; Yuma Aoki; Masaki Kakehi; Shunsuke Mori; Tomomi Takano; Yukihiko Kubota; Hon-Song Kim; Yukimasa Shibata; Kiyoji Nishiwaki
PloS one, 2020
During development of the Caenorhabditis elegans gonad, the gonadal leader cells, called distal tip cells (DTCs), migrate in a U-shaped pattern to form the U-shaped gonad arms. The ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs) family metalloproteases MIG-17 and GON-1 are required for correct DTC migration. Mutations in mig-17 result in misshapen gonads due to the misdirected DTC migration, and mutations in gon-1 result in shortened and swollen gonads due to the premature termination of DTC migration. Although the phenotypes shown by mig-17 and gon-1 mutants are very different from one another, mutations that result in amino acid substitutions in the same basement membrane protein genes, emb-9/collagen IV a1, let-2/collagen IV a2 and fbl-1/fibulin-1, were identified as genetic suppressors of mig-17 and gon-1 mutants. To understand the roles shared by these two proteases, we examined the effects of the mig-17 suppressors on gon-1 and the effects of the gon-1 suppressors and enhancers on mig-17 gonadal defects. Some of the emb-9, let-2 and fbl-1 mutations suppressed both mig-17 and gon-1, whereas others acted only on mig-17 or gon-1. These results suggest that mig-17 and gon-1 have their specific functions as well as functions commonly shared between them for gonad formation. The levels of collagen IV accumulation in the DTC basement membrane were significantly higher in the gon-1 mutants as compared with wild type and were reduced to the wild-type levels when combined with suppressor mutations, but not with enhancer mutations, suggesting that the ability to reduce collagen IV levels is important for gon-1 suppression. - Control of the basement membrane and cell migration by ADAMTS proteinases: Lessons from C. elegans genetics
Hon-Song Kim; Kiyoji Nishiwaki
MATRIX BIOLOGY, May 2015, [Reviewed] - The BED finger domain protein MIG-39 halts migration of distal tip cells in Caenorhabditis elegans
Tetsuhiro Kikuchi; Yukimasa Shibata; Hon-Song Kim; Yukihiko Kubota; Sawako Yoshina; Shohei Mitani; Kiyoji Nishiwaki
DEVELOPMENTAL BIOLOGY, Jan. 2015, [Reviewed] - The Novel Secreted Factor MIG-18 Acts with MIG-17/ADAMTS to Control Cell Migration in Caenorhabditis elegans
Hon-Song Kim; Yuko Kitano; Masataka Mori; Tomomi Takano; Thomas Edward Harbaugh; Kae Mizutani; Haruka Yanagimoto; Sayaka Miwa; Shinji Ihara; Yukihiko Kubota; Yukimasa Shibata; Kohji Ikenishi; Gian Garriga; Kiyoji Nishiwaki
GENETICS, Feb. 2014, [Reviewed] - The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell migration in Caenorhabditis elegans
Hon-Song Kim; Yuko Kitano; Masataka Mori; Tomomi Takano; Thomas Edward Harbaugh; Kae Mizutani; Haruka Yanagimoto; Sayaka Miwa; Shinji Ihara; Yukihiko Kubota; Yukimasa Shibata; Kohji Ikenishi; Gian Garriga; Kiyoji Nishiwaki
Genetics, 2014, [Reviewed] - Nuclear positioning in the gonadal distal tip cells of C. elegans.
Kim HS; Nishiwaki K
Worm, Apr. 2012, [Reviewed] - VAB-10 spectraplakin acts in cell and nuclear migration in Caenorhabditis elegans
Hon-Song Kim; Ryoko Murakami; Sophie Quintin; Masataka Mori; Kiyotaka Ohkura; Katsuyuki K. Tamai; Michel Labouesse; Hiroshi Sakamoto; Kiyoji Nishiwaki
DEVELOPMENT, Sep. 2011, [Reviewed] - Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex
Hon-Song Kim; Mikiko Takahashi; Kazuhiko Matsuo; Yoshitaka Ono
GENES TO CELLS, Mar. 2007, [Reviewed] - Centrosome-targeting region of CG-NAP causes centrosome amplification by recruiting cyclin E-cdk2 complex
T Nishimura; M Takahashi; HS Kim; H Mukai; Y Ono
GENES TO CELLS, Jan. 2005, [Reviewed] - Regulation of a mitogen-activated protein kinase kinase kinase, MLTK by PKN
M Takahashi; Y Gotoh; T Isagawa; T Nishimura; E Goyama; HS Kim; H Mukai; Y Ono
JOURNAL OF BIOCHEMISTRY, Feb. 2003, [Reviewed]
MISC
Research Themes
- Neuropeptide regulation of organogenesis through cell surface FoF1-ATP synthase
Grant-in-Aid for Scientific Research (C)
Kwansei Gakuin University
01 Apr. 2022 - 31 Mar. 2025 - CG-NAP function in ciliogenesis through regulation of centriolar satellite formation
Grant-in-Aid for Scientific Research (C)
Teikyo Heisei University
01 Apr. 2019 - 31 Mar. 2022 - Role of a ribosomal protein in the ADAMTS protease pathway regulating cell migration
Grant-in-Aid for Challenging Exploratory Research
Kwansei Gakuin University
01 Apr. 2014 - 31 Mar. 2017
MIG-17, an ADAMTS metalloprotease controls migration of gonadal distal tip cells (DTCs) through regulation of extracellular matrix in C. elegans. In the present study, we asked why the mutation in a ribosomal protein RPL-20(G82R) can suppress the DTC migration defect in the mig-17 mutants. Although we could not reach a clear understanding of the mechanism, we found that RPL-20(G82R) does not affect the regulatory mechanism of translational initiation. We also showed that RPL-20(G82R) is not secreted, but that it appears to act through unknown secreted proteins to suppress the mig-17 defects. We found that the RPL-20 activity is especially important in the intestine for the suppression to occur. - Analysis of flp-10 that controls organogenesis in c.elegans
Grant-in-Aid for Challenging Exploratory Research
Kwansei Gakuin University
01 Apr. 2013 - 31 Mar. 2015
An FMRFamide has Phe-Met-Arg-Phe-NH2 in its carboxyl terminus. These peptides are known to be involved in regulation of neuronal activities. We found that the C.elegans FLP-10C peptide, a part of the proteolytic fragment of FMRFamide like peptide (FLP), controls tip cell migration of U-shaped gonads in C.elegans. This suggests a novel function of this peptide in regulating gonadogenesis. We identified a protein in the worm lysate specifically co-precipitated with the FLP-10C peptide in a pull-down assay using the biotinylated peptide. LC/MS/MS analysis revealed that the specific protein corresponded to the product of H28O16.1. H28O16.1 encodes the alpha subunit of mitochondrial ATP synthase. Interestingly, there are some reports that the mitochondrial ATP synthase is also expressed in the plasma membrane. These results suggest that H28O16.1 could be the receptor for the FLP-10C peptide to regulate gonadogenesis.