Keiji Maruyama

Faculty of Pharmaceutical Sciences,Department of Pharmaceutical SciencesProfessor
Graduate School of Pharmaceutical Sciences,Doctoral Program in Pharmaceutical SciencesProfessor
Last Updated :2025/10/07

■Researcher basic information

Field Of Study

  • Life sciences, Clinical pharmacy

■Career

Career

  • Apr. 2024 - Present
    Teikyo Heisei University, Faculty of Pharmaceutical Sciences
  • Apr. 2007
    Teikyo University, Faculty of Pharma-Science
  • Nov. 2005
  • Apr. 1998
  • Aug. 1997
  • Apr. 1993

Educational Background

  • Mar. 2004, Showa University, Guraduate School, of Pharmaceutical Sciences
  • Mar. 1993, Showa University, Guraduate School, of Pharmaceutical Sciences

Member History

  • Apr. 2014 - Mar. 2016
  • Apr. 2012 - Mar. 2014

■Research activity information

Paper

  • メタボリックシンドロームモデルマウス(TSODマウス)における黒酢濃縮物および黒酢もろみ末の高血糖抑制作用　　　　　　　　　　　　　　　
    Dec. 2024, [Reviewed]
  • 薬科大学の新シミュレーション教育(第1回) 薬系大学で行われている新しいシミュレーション教育 問題解決能力育成のためのシミュレーション教育　　　　　　　　　　　　　　　
    Nov. 2023, [Invited]
  • 薬学部学生に対するドキュメンタリー映像を用いた倫理教育の実践
    Jul. 2023, [Reviewed]
    URL
  • A Complex of Type I Platelet-Activating Factor Acetylhydrolase (PAF-AH) Catalytic Subunits Switches from α1/α2 Heterodimer to α2/α2 Homodimer during Adipocyte Differentiation of 3T3-L1 Cells.
    Yasuhiro Nakamura; Fumiko Kihara-Negishi; Kazunari Tanigawa; Mitsuo Kiriya; Yuki Kadowaki; Haruka Imagawa; Hiroki Nakanishi; Shigekazu Watanabe; Keiji Maruyama; Ken Karasawa
    Biological & pharmaceutical bulletin, 2023, [Reviewed]
    Platelet-activating factor acetylhydrolase (PAF-AH) hydrolyzes an acetyl ester at the sn-2 position of platelet-activating factor (PAF), thereby mediating a variety of biological functions. PAF-AH is found in three isoforms: Type I PAF-AH (PAF-AH I) and Type II PAF-AH (PAF-AH II) are intracellular enzymes whereas plasma PAF-AH is characterized by association with lipoprotein in plasma. PAF-AH I forms a tetramer constituted by two catalytic subunits (α1 and α2) with β regulatory subunits. We recently showed that a deficiency of PAF-AH I catalytic subunits in male mice caused an increase of body weight, food intake, and white adipose tissue (WAT) weight. In this study, we examined whether the expression of this enzyme was altered in the differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of PAF-AH I α1 subunit protein was significantly reduced in 3T3-L1 differentiation, while the amount of the PAF-AH I α2 subunit was not changed. Immunoprecipitation analysis of 3T3-L1 differentiation showed that the complex of PAF-AH I catalytic subunits was changed from α1/α2 heterodimer to α2/α2 homodimer. Our findings suggest that changes in PAF-AH I catalytic subunits are involved in adipocyte differentiation of 3T3-L1 and obesity in mice.
    DOI URL
  • Genome-wide screening identified SEC61A1 as an essential factor for mycolactone-dependent apoptosis in human premonocytic THP-1 cells.
    Akira Kawashima; Mitsuo Kiriya; Junichiro En; Kazunari Tanigawa; Yasuhiro Nakamura; Yoko Fujiwara; Yuqian Luo; Keiji Maruyama; Shigekazu Watanabe; Masamichi Goto; Koichi Suzuki
    PLoS neglected tropical diseases, Aug. 2022, [Reviewed]
    Buruli ulcer is a chronic skin disease caused by a toxic lipid mycolactone produced by Mycobacterium ulcerans, which induces local skin tissue destruction and analgesia. However, the cytotoxicity pathway induced by mycolactone remains largely unknown. Here we investigated the mycolactone-induced cell death pathway by screening host factors using a genome-scale lenti-CRISPR mutagenesis assay in human premonocytic THP-1 cells. As a result, 884 genes were identified as candidates causing mycolactone-induced cell death, among which SEC61A1, the α-subunit of the Sec61 translocon complex, was the highest scoring. CRISPR/Cas9 genome editing of SEC61A1 in THP-1 cells suppressed mycolactone-induced endoplasmic reticulum stress, especially eIF2α phosphorylation, and caspase-dependent apoptosis. Although previous studies have reported that mycolactone targets SEC61A1 based on mutation screening and structural analysis in several cell lines, we have reconfirmed that SEC61A1 is a mycolactone target by genome-wide screening in THP-1 cells. These results shed light on the cytotoxicity of mycolactone and suggest that the inhibition of mycolactone activity or SEC61A1 downstream cascades will be a novel therapeutic modality to eliminate the harmful effects of mycolactone in addition to the 8-week antibiotic regimen of rifampicin and clarithromycin.
    DOI URL
  • Thyroid stimulating hormone suppresses the expression and activity of cytosolic sulfotransferase 1a1 in thyrocytes.
    Yasuhiro Nakamura; Aya Yoshihara; Mitsuo Kiriya; Akira Kawashima; Kazunari Tanigawa; Yuqian Luo; Yoko Fujiwara; Keiji Maruyama; Shigekazu Watanabe; Fumiko Kihara-Negishi; Ken Karasawa; Koichi Suzuki
    Endocrine journal, 09 Jun. 2022, [Reviewed]
    Sulfonation is an important step in the metabolism of dopamine, estrogens, dehydroepiandrosterone, as well as thyroid hormones. However, the regulation of cytosolic sulfotransferases in the thyroid is not well understood. In a DNA microarray analysis of rat thyroid FRTL-5 cells, we found that the mRNA expression of 10 of 48 sulfotransferases was significantly altered by thyroid stimulating hormone (TSH), with that of sulfotransferase family 1A member 1 (SULT1A1) being the most significantly affected. Real-time PCR and Western blot analyses revealed that TSH, forskolin and dibutyryl cyclic AMP significantly suppressed SULT1A1 mRNA and protein levels in a time- and concentration-dependent manner. Moreover, immunofluorescence staining of FRTL-5 cells showed that SULT1A1 is localized in the perinuclear area in the absence of TSH but is spread throughout the cytoplasm with reduced fluorescence intensity in the presence of TSH. Sulfotransferase activity in FRTL-5 cells, measured using 3'-phosphoadenosine-5'-phosphosulfate as a donner and p-nitrophenol as an acceptor substrate, was significantly reduced by TSH. These findings suggest that the expression and activity of SULT1A1 are modulated by TSH in thyrocytes.
    DOI URL
  • Nobiletin and 3'-Demethyl Nobiletin Activate Brown Adipocytes upon β-Adrenergic Stimulation.
    Fumiko Kihara-Negishi; Naoki Ohkura; Yuka Takahashi; Toshiyuki Fujita; Yasuhiro Nakamura; Keiji Maruyama; Tetsuta Oshitari; Shinji Yamaguchi
    Biological & pharmaceutical bulletin, 2022, [Reviewed]
    Brown adipose tissue (BAT) specifically regulates energy expenditure via heat production. Nobiletin (NOB), a natural polymethoxylated flavone present in citrus fruits, can activate thermogenesis in the BAT of high-fat diet-induced obese mice. The activity of BAT is directly regulated by β-adrenergic stimulation. In this study, we report the effects of NOB on BAT activation using β-adrenergic agonists. We observed that when HB2 brown adipocyte cell lines are stimulated with β-adrenergic agonists, NOB enhances the expression of uncoupling protein 1 (UCP1), which is associated with the mitochondrial energy metabolism in these cells. Moreover, NOB increases the mRNA expression of the brown adipokines neuregulin-4 (Nrg4) and fibroblast growth factor-21 (FGF-21) and the secretion of FGF-21. These results suggest that NOB can enhance the thermogenic functions of brown adipocytes and promote brown adipokine secretion due to enhanced β-adrenergic stimulation. In addition, 3'-demethyl nobiletin (3'-DMN), an NOB CYP-enzyme metabolite, can increase UCP1 mRNA expression. Both NOB and 3'-DMN significantly promoted mitochondrial membrane potential in HB2 adipocytes following β-adrenergic stimulation. Therefore, we believe that NOB could be a promising candidate for activating BAT under β-adrenergic stimulation and preventing the onset of obesity.
    DOI URL
  • 薬学生に対する服薬模擬体験学習の有用性の検討
    Aug. 2021, [Reviewed]
  • Deficiency of Type I Platelet-Activating Factor-Acetylhydrolase Catalytic Subunits Causes an Increase in Body Weight.
    Yasuhiro Nakamura; Momoko Yoshida; Kazunari Tanigawa; Ayako Harada; Fumiko Kihara-Negishi; Keiji Maruyama; Ken Karasawa
    Biological & pharmaceutical bulletin, 2021, [Reviewed]
    Type I platelet-activating factor-acetylhydrolase (PAF-AH) forms a complex consisting of two catalytic subunits (α1 and/or α2) with a regulatory subunit (β). Although this protein was discovered as an enzyme that degrades an acetyl ester linked at the sn-2 position of platelet-activating factor (PAF), its physiological function remains unknown. In this study, to examine whether knockout mice lacking the catalytic subunits of this enzyme showed a different phenotype from that of wild-type mice, we measured and compared the body weights of knockout mice and control mice. The body weights of knockout mice were significantly increased compared to those of the control mice during 6 to 20 weeks from birth. Food intake was also significantly increased in knockout mice compared with control mice during these periods. Since a decrease in testis weight was reported in the knockout mice, we expected a decrease in testosterone levels. We measured and compared the amounts of testosterone in the serum and testis of knockout and control mice using liquid chromatography-tandem mass spectrometry, and found that testosterone levels in both the serum and testis were significantly decreased in the knockout mice compared with the control mice. These results suggest that a deficiency of type I PAF-AH catalytic subunits causes an increase in body weight, in part, due to reduced testosterone levels in male mice.
    DOI URL
  • 大学生のアンチ・ドーピングの知識に関する調査 体育系大学と薬学系大学の比較
    Aug. 2020, [Reviewed]
  • フィードバック装置を用いた薬学部一年生における一次救命処置実習の学修効果
    Jun. 2020, [Reviewed]
    URL
  • リアルタイムフィードバック装置を用いた胸骨圧迫手技の男女別学修効果の比較
    Apr. 2020, [Reviewed]
    URL
  • Analysis of basic life support training provided to pharmacy students using feedback device
    Keiji Maruyama; Yasuo Takeuchi; Naoki Ohkura; Fumiko Kihara-Negishi; Nobu Akiyama; Ichiro Kaneko
    Yakugaku Zasshi, 2020
    DOI URL
  • Possible antithrombotic properties of propolis
    Naoki Ohkura; Keiji Maruyama; Fumiko Negishi
    Journal of Apitherapy, 2020, [Reviewed]
    URLDOI URL
  • Comparison of the longevity of the anesthetic effect between lidocaine/propitocaine cream and in–hospital formulations
    Hisayo Yamaoka; Yoshito Ishiki; Moemi Saito; Keiji Maruyama; Tamaki Watanabe; Kazuo Maruyama; Ryo Suzuki; Shigekaze Watanabe; Makoto Yasuda; Tomoya Mukouyama; Tomoyo Yamanobe; Keiko Yamaoka; Nobuhiro Yasuno; Yuzo Komuro
    Therapeutic Research, Oct. 2018, [Reviewed]
    Scopus URLScopus Citedby URL
  • Simultaneous Determination of Catechins and Caffeine in Green Tea-Based Beverages and Foods for Specified Health Uses
    Keiji Maruyama; Fumiko Kihara-Negishi; Naoki Ohkura; Yasuhiro Nakamura; Miwako Nasui; Moemi Saito
    Food and Nutrition Sciences, Mar. 2017, [Reviewed]
    URLDOI URL
  • Caffeine-containing beverages served to patients on psychiatric wards
    Jul. 2016, [Reviewed]
    URL
  • 4 種類のリドカイン製剤（院内製剤）における基剤の検討
    Feb. 2014, [Reviewed]
    DOI URL
  • 認知行動療法と動機付け面接法を用いた禁煙指導実習の構築
    Mar. 2012, [Reviewed]
    DOI URL
  • リマプロストアルファデクス錠の後発医薬品に関する品質評価
    Mar. 2010, [Reviewed]
    DOI URL
  • 帝京大学病院小児科外来における小児患者のサプリメント・健康食品の使用状況
    Feb. 2010, [Reviewed]
    DOI URL
  • 「患児保護者への薬に関するアンケート結果」に対する小児科医の評価　　　　　　　　　　　　　　　
    Dec. 2009, [Reviewed]

MISC

Books and other publications

  • 医薬品情報学
    Contributor
    Jan. 2025
    9784758322249
    CiNii Books URL
  • セルフメディケーションとOTC医薬品 : 代表的な疾患における処方とその背景
    Editor
    Sep. 2020
    9784909197757
    CiNii Books URL
  • 薬剤師業務の基本[知識・態度]第3版　　　　　　　　　　　　　　　
    Joint work
    Dec. 2017
  • 医薬品情報学　　　　　　　　　　　　　　　
    Joint work
    Mar. 2017
  • セルフケアとOTC医薬品　　　　　　　　　　　　　　　
    Joint work
    Mar. 2017
  • 早期臨床体験テキスト　　　　　　　　　　　　　　　
    Joint work
    Mar. 2017
  • 図解 医薬品情報学　　　　　　　　　　　　　　　
    Joint work
    Jan. 2014
  • 薬学生のための実務実習事前学習テキスト　　　　　　　　　　　　　　　
    Joint work
    Aug. 2013
  • 医薬品情報評価学　　　　　　　　　　　　　　　
    Joint work
    Mar. 2013
  • 薬学生のための臨床実習マニュアル　　　　　　　　　　　　　　　
    Joint work
    Mar. 2011
  • 改訂 わかりやすいセルフメディケーションとOTC医薬品の使い方　　　　　　　　　　　　　　　
    Joint work
    Mar. 2011

Affiliated academic society

  • JAPANESE SOCIETY OF PHARMACEUTICAL HEALTH CARE AND SCIENCES　　　　　　　　　　　　　　　
  • THE PHARMACEUTICAL SOCIETY OF JAPAN